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Synergistic precipitant powered self-assembly of papain for cross-linked enzyme crystals preparation

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Particuology 2024年第2期85卷 102-112页

作者： Qi Hao Pan Guo Honghai Wang Min su Chunli Li Yuqi Hu Weiyi su School of Chemical Engineering and Technology Hebei University of TechnologyTianjin300130China National-Local Joint Engineering Laboratory for Energy Conservation of Chemical Process Integration and Resources Utilization Tianjin300130China

In this study,we prepared cross-linked enzyme crystals(CLECs)of papain to further broaden the application of the enzyme with high activity in extreme ***,papain crystals were successfully obtained based on the micro-batch,batch,and expanded batch crystallization ***,ammonium sulfate and polyethylene glycol 6000(PEG6000)were synergistically used as the precipitants,while L-cysteine was applied to enhance the activity of ***,the interaction between L-cysteine and papain was modeled by molecular docking *** was found that L-cysteine could form a hydrogen bond with aspartic acid residue(Asp)at site 158,and the electrostatic attraction with lysine residue(Lys)at site 156 was also quite *** the enzyme crystals were cross-linked by glutaraldehyde at optimized *** papain CLECs were identified by various methods,and it was found that the thermal stability and enzymatic activity both increased compared to the raw *** importantly,it could be applied at more rigorous conditions,for example,pH of 4.

关键词： Papain Cross-linked enzyme crystals L-cysteine Molecular docking Crystallization

Recurrent malignant hyperthermia after scoliosis correction surgery

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World Journal of Emergency Medicine 2024年第1期15卷 70-72页

作者： Junfeng su Man Huang Department of General Intensive Care Unit the Second Affi liated Hospital of Zhejiang University School of MedicineHangzhou 310052China Key Laboratory of Early Warning and Intervention of Multiple Organ Failure Ministry of Education of the People's Republic of ChinaHangzhou 310052China

Malignant hyperthermia(MH)is a genetic disorder of skeletal muscle cells that affects muscle cytoplasmic calcium homeostasis,with high mortality and low ***,it presents with non-specific signs of a hypermetabolic response,including high fever,tachycardia,and elevated end-tidal carbon dioxide(ETCO_(2)).The successful treatment lies in the timely recognition and early use of dantrolene.[1]As an inhibitor of Ca2+release through ryanodine receptor(RYR)channels,the skeletal muscle relaxant dantrolene has proven to be both a valuable experimental probe of intracellular Ca2+signaling and a lifesaving treatment for MH.[2]Dominant mutations in the skeletal muscle RYR1 gene are well-recognized causes of both malignant hyperthermia susceptibility(MHS)and central core disease(CCD).

关键词： hyperthermia malignant elevated

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Mass-produced and uniformly luminescent photochromic fibers toward future interactive wearable displays

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Light(Science & Applications) 2024年第4期13卷 593-595页

作者： Yan Li Yikai su Department of Electronic Engineering Shanghai Jiao Tong University200240 ShanghaiChina

Enabling flexible fibers with light-emitting capabilities has the potential to revolutionize the design of smart wearable interactive devices.A recent publication in Light Science&Application,an interdisciplinary team of scientists led by ***-Qing Lu and *** Tao has realized a highly flexible,uniformly luminescent photochromic fiber based on a mass-produced thermal drawing *** overcomes the shortcomings of existing commercial lightdiffusing fibers,exhibiting outstanding one-dimensional linear illumination *** research team integrated controllable photochromic fibers into various wearable interaction interfaces,providing a novel approach and insights to enable human-computer interaction.

关键词： interaction fibers uniformly

Impairment of Autophagic Flux After Hypobaric Hypoxia Potentiates Oxidative Stress and Cognitive Function Disturbances in Mice

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Neuroscience Bulletin 2024年第1期40卷 35-49页

作者： Shuhui Dai Yuan Feng Chuanhao Lu hongchen Zhang Wenke Ma Wenyu Xie Xiuquan Wu Peng Luo Lei Zhang Fei Fei Zhou Fei Xia Li Department of Neurosurgery Xijing HospitalFourth Military Medical UniversityXi'an 710000China National Translational Science Center for Molecular Medicine and Department of Cell Biology Fourth Military Medical UniversityXi'an 710000China Department of Neurosurgery The Central Hospital of BaojiBaoji 721000China Department of Ophthalmology Xijing HospitalFourth Military Medical UniversityXi'an 710000China

Acute hypobaric hypoxic brain damage is a potentially fatal high-altitude *** plays a critical role in ischemic brain injury,but its role in hypo-baric hypoxia(HH)remains *** we used an HH chamber to demonstrate that acute HH exposure impairs autophagic activity in both the early and late stages of the mouse brain,and is partially responsible for HH-induced oxidative stress,neuronal loss,and brain *** autophagic agonist rapamycin only promotes the initiation of *** proteome analysis,a screen showed that protein dynamin2(DNM2)potentially regulates autophagic *** of DNM2 significantly increased the formation of autolysosomes,thus maintaining autophagic flux in combination with ***,the enhancement of autophagic activity attenuated oxidative stress and neurological deficits after HH *** results contribute to evidence supporting the conclusion that DNM2-mediated autophagic flux represents a new therapeutic target in HH-induced brain damage.

关键词： Autophagy-Proteomics Oxidative stress Hypobaric hypoxia-Brain injury

Divergent responses of runoff to climate change in the upper basins of the Third Pole dominated by westerlies and monsoon

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Science China Earth Sciences 2024年第8期67卷 2592-2614页

作者： Qikai suN Fengge su He suN State Key Laboratory of Tibetan Plateau Earth System Environment and Resources(TPESER)Institute of Tibetan Plateau ResearchChinese Academy of SciencesBeijing 100101China University of Chinese Academy of Sciences Beijing 100101China

The diverse climates,distribution of snow and glaciers,and geographic locations directly affect the runoff response to climate change in the upper basins of the Third *** present,a comprehensive analysis of runoff variations and their distinct responses to climate change in the westerlies-and monsoon-dominated upper basins is still *** study comprehensively analyzed annual runoff variations in westerlies-dominated basins(the upper basins of the Aksu(UAKS),Syr Darya(USRD),Yarkant(UYK),Hotan(UHT),Amu Darya(UAMD),and Indus(UI))and monsoon-dominated basins(the upper basins of the Yangtze(UYA),Yellow(UYE),Lancang(ULC),Nujiang(UNJ),and Yarlung Zangbo(UYZ))of the Third Pole from 1961 *** multi-source meteorological data and large-scale circulation factors,this study investigated the divergent responses of runoff in the upper basins to climate change,and explored the large-scale circulation mechanisms underlying runoff variations in these upper *** results showed that:(1)The annual runoff in the majority of upper basins(except for the UYE and UYZ)exhibited an increasing trend,and the annual runoff in the UAKS,UYK,and UI showed a significant increasing trend from1961 to *** annual runoff in the upper basins of the Third Pole changed abruptly from decreasing to increasing between the 1980s and 2000s,with the exception of the UYE.(2)The runoff in the monsoon-dominated upper basins has been controlled primarily by changes in precipitation over the past 55 *** contrast,the runoff in the westerlies-dominated upper basins exhibited three distinct long-term responses to climate change:temperature-dominated(UYK and UHT),precipitation-dominated(USRD and UAMD),and the combined influence of precipitation and temperature(UAKS and UI).Since the 1960s,the sensitivity of runoff to warm season temperature changes in the most westerlies-dominated upper basins has decreased,while the response of runoff to precipitation changes has intensified.(3)The stud

关键词： Upper basins of the Third Pole Climate change Westerlies and monsoon Runoff variations Large-scale circulation factors

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An improved bound on accelerated light dark matter

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Science China(Physics,Mechanics & Astronomy) 2024年第2期67卷 105-118页

作者： Liangliang su Lei Wu Bin Zhu Department of Physics and Institute of Theoretical Physics Nanjing Normal UniversityNanjing 210023China School of Physics Yantai UniversityYantai 264005China

Light(sub-GeV)dark matter has gained increasing interest in terms of direct *** dark matter is a promising candidate that can generate detectable nuclear recoil energy within the sub-GeV *** of the large kinetic energy,its interactions with the nucleus are predominantly governed by inelastic scattering,including quasi-elastic and deep inelastic *** this work,we calculated the inelastic effects in dark matter-Earth scattering mediated by a vector *** analysis revealed that the impact of inelastic scattering relies on the mediator mass and the kinetic energy spectrum of dark *** results exhibited considerable disparity:the upper bounds of the exclusion limit for the spin-independent cross-section between accelerated dark matter and nuclei via a heavy mediator differ by several tens of times when inelastic scattering is considered.

关键词： accelerated dark matter inelastic scattering direct detection experiment

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Multiscale observation in wide-spatial radar surveillance based on coherent FDA design

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Science China(Information Sciences) 2024年第2期67卷 232-252页

作者： Lei YU Feng HE Yi su College of Electronics Science National University of Defense Technology

Wide-spatial radar surveillance missions are challenging tasks, requiring an increased power budget and agility in transmission aimed at extracting information from multiple targets in different environments. These requirements necessitate high transmitting degrees of freedom(DOF) to achieve the objective of multiscale observation for specific tasks in specialized regions of interest. Herein, we exploit the multiscale observation ability in wide-spatial radar surveillance based on frequency diverse array(FDA)radar. The proposed method facilitates spatial anisotropic control of multiple radar resources, including the transmitting waveforms, beampattern, and bandwidth. By utilizing a coherent FDA radar, we offer principles for the selection of baseband waveforms, in addition to the quantitative design of the beampattern gain and optimal bandwidth from the perspective of detection. The feasibility of the proposed method is validated through numerical experiments, thus indicating the potential in wide-spatial radar surveillance. Moreover,this work can be regarded as a preliminary attempt to gauge the efficacy of the computational array, a novel academic concept.

关键词： multi-scale observation wide-spatial surveillance frequency diverse array (FDA) beampattern synthesis frequency diversity gain computational array

GeoChip-based microbial functions in biogeochemical cycles and their responses to environmental factors in Tengchong hot springs

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Journal of Earth Science 2024年

作者： Muhammad Inayat Ullah Khan Liuqin Huang Geng Wu Jian Yang Xiangyu Guan hongchen Jiang School of Environmental Studies China University of Geosciences School of Ocean Sciences China University of Geosciences Beijing State Key Laboratory of Biogeology and Environmental Geology China University of Geosciences

Microorganisms actively participate in biogeochemical cycling processes and play a crucial role in maintaining the dynamic balance of hot spring ecosystems. However， the distribution of microbial functional genes and their influencing factors in hot springs remain largely unclear. Therefore， this study investigated the microbial functional genes and their potential for controlling biogeochemical cycles (C， N， S， and P) in the hot Springs of Tengchong， China， using the Geochip method， a functional gene microarray technology. The examined hot springs have very different microbial functional genes. A total of 22736 gene probe signals were identified， belonging to 567 functional genes and associated with 15 ecological functions， mainly involving stress response， carbon cycle， nitrogen cycle， sulfur cycle， phosphorus cycle and energy processes. The amyA， narG， dsrA and ppx genes were most abundant in carbon， nitrogen， sulfur and phosphorus cycles， respectively， and were significantly correlated with pH， temperature and SO42-. The diversity and abundance of detected gene probes were negatively correlated with temperature. The α-diversity (i.e.， Shannon index) was high at low temperature and low pH. Molecular functional interactions revealed by the gene connectivity levels were negatively correlated with temperature， pH and SO42-. These results suggested that the abundance， diversity and interactions of microbial functional genes were significantly influenced by geochemical parameters?. In addition， some genera possessed functional genes related to carbon， nitrogen， sulfur， and phosphorus cycles and can synergistically control the biogeochemical cycles of carbon， nitrogen， sulfur and phosphorus. These findings provide new insights into the functional potentials of microorganisms to participate in biogeochemical cycles and their responses to environmental factors in hot springs.

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A real-world study of the differences in Traditional Chinese Medicine diagnosis and treatment rules for coronavirus disease 2019 between Northern and Southern China

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Journal of Traditional Chinese Medicine 2024年第4期44卷 822-829页

作者： su Rui su Youzhu WANG Shuo FAN Jie LIU Qingquan LIU Mifeng Department of infectious diseases Capital Medical University Beijing Hospital of Traditional Chinese Medicine

OBJECTIVE: To explore the differences in Traditional Chinese Medicine(TCM) diagnosis and treatment rules for coronavirus disease 2019(COVID-19) between Northern and Southern China based on the real-world data from 982 COVID-19 patients. METHODS: All consecutive cases of COVID-19 admitted to the TCM department of designated COVID-19 hospitals in eight provinces and cities were retrospectively analyzed. Patients were divided into a Northern and a Southern group according to the location of the admitting hospital. The symptoms, syndrome elements, syndrome distribution and herbal treatments were analyzed. The core prescriptions were extracted using the multiscale backbone-based network comparison algorithm(msbNC). REsuLTS: The distribution of syndrome elements showed that dampness was common in Northern and Southern China, wind and heat were more often present in the South, while fire toxin and spleen deficiency were more often encountered in the North. The distribution of syndromes showed that the South was dominated by heat dampness accumulating in the lung(55.69%), while the North was dominated by dampness-toxin stagnating in the lung(44.90%).The results of core prescription mining showed that dispelling dampness, dispersing wind, clearing heat and strengthening spleen were the common treatment methods in Northern and Southern China. For mild cases, Jinyinhua(Flos Lonicerae) and Lianqiao(Fructus Forsythiae suspensae) were often used in the South to clear heat and relieve exterior symptoms, while Chaihu(Radix Bupleuri Chinensis) and Huangqin(Radix Scutellariae Baicalensis) were often used in the North to relieve muscles by expelling heat. For moderate cases, Chaihu(Radix Bupleuri Chinensis), Qinghao(Herba Artemisiae Annuae), and Shigao(Gypsum Fibrosum) were often used to clear heat of Tri-jiao Channel and stomach in the South, while Fuling(Poria), Chenpi(Pericarpium Citri Reticulatae), and Dangshen(Radix Codonopsis) were often used to invigorate spleen and remove damp

关键词： COVID-19 North-South difference core prescription medicine, Chinese traditional retrospective studies syndrome