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Cul.ure conditions for equine bone marrow mesenchymal.stem cel.s and expression of key transcription factors during their differentiation into osteobl.sts

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Journal.of Animal.Science and Biotechnol.gy 2014年第2期5卷 163-172页

作者： El.zabeth R A Gl.nn Al.redo Sanchez l.ndono steven A Zinn Thomas A Hoagl.Kristen E Govoni Department of Animal.Science University of Connecticut3636 Horsebarn Road Ext.Unit 4040StorrsCT 06269-4040USA Department of Environmental.and Popul.tion Heal.h Tufts University North GraftonMA 01536USA

Background： The use of equine bone marrow mesenchymal.stem cel.s （BMSC） is a novel.method to improve fracture heal.ng in horses. However, additional.research is needed to identify optimal.cul.ure conditions and to determine the mechanisms invol.ed in regul.ting BMSC differentiation into osteobl.sts. The objectives of the experiments were to determine： 1） if autol.gous or commercial.serum is better for prol.feration and differentiation of equine BMSC into osteobl.sts, and 2） the expression of key transcription factors during the differentiation of equine BMSC into osteobl.sts. Equine BMSC were isol.ted from the sterna of 3 horses, treated with purchased fetal.bovine serum （FBS） or autol.gous horse serum （HS）, and cel. prol.feration determined. To induce osteobl.st differentiation, cel.s were incubated with l.ascorbic acid-2-phosphate and gl.cerol.2-phosphate in the presence or absence of human bone morphogenetic protein2 （BMP2）, dexamethasone （DEX）, or combination of the two. Al.al.ne phosphatase （Al.） activity, a marker of osteobl.st differentiation, was determined by El.SA. Total.RNA was isol.ted from differentiating BMSC between d 0 to 18 to determine expression of runt-rel.ted tronscrJption foctor2 （Runx2）, osterix （Osx）, and T-box3 （Tbx3）. Data were anal.zed by ANOVA. Resul.s： Rel.tive to control. FBS and HS increased cel. number （133 ± 5 and 116 ± 5%, respectivel.; P 〈 0.001） and 5-bromo- 2＇-deoxyuridine （BrdU） incorporation （167 ± 6 and 120 ± 6%, respectivel.; P 〈 0.001）. Treatment with DEX increased Al. activity compared with control.（1,638 ± 38%; P 〈 0.001）. In the absence and presence of Dex, BMP-2 did not al.er Al. activity （P 〉 0.8）. Runt-rel.ted transcription foctor2 expression increased 3-fol. （P 〈 0.001） by d 6 of cul.ure. Osterix expression increased 94ol. （P 〈 0.05） by d 18 of cul.ure. Expression of Tbx3 increased 1.8-fol. at d 3 （P 〈 0.01）; however expression was reduced 4-fol. at d 18 （P 〈 0.01）. Concl.sions： Dexamethasone, but not BMP-2, is requir

关键词： Bone marrow mesenchymal.stem cel.s Cel. cul.ure Equine Osteobl.sts Transcription factors

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Personal.Exposure to Fine Particul.tes and Pol.cycl.c Aromatic Hydrocarbons in an Office Environment in Xi＇an, China

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环境科学前沿（中英文版） 2013年第4期2卷 33-45页

作者： Hongmei Xu Junji Cao Meil.ng Gao Kin Fai Ho Xinyi Niu Teresa l. Coons steven Sai Hang H0 Gehui Wang Zhuzi Zhao Key l.b of Aerosol.Science & Technol.gy SKLLQG Institute of Earth Environment Chinese Academy of Sciences Xi'an710075 China University of Chinese Academy of Sciences Beijing 100049 China Institute of Gl.bal.Environmental.Change Xi'an Jiaotong University Xi'an 710054 China University of Cal.fornia Berkeley USA School.of Publ.c Heal.h and Primary Care The Chinese University of Hong Kong Hong Kong China Washington State University USA Hong Kong Premium Services and Research l.boratory Lai Chi Kok Kowloon Hong Kong China

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Regul.tion of cel.ul.r anabol.sm by mTOR:or how I l.arned to stop worrying and l.ve transl.tion

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Sports Medicine and Heal.h Science 2020年第4期2卷 195-201页

作者： J.Wil.iam Deaver Sara Mata l.pez Patrick J.Ryan Peter P.Nghiem steven E.Riechman James D.Fl.ckey Department of Heal.h and Kinesiol.gy 107 Gilchrist Building2929 Research ParkwayTexas A&M UniversityCollege StationTXUSA Department of Veterinary Integrative Biosciences 402 Raymond Stotzer Pkwy Building 2Texas A&M UniversityCollege StationTXUSA

The process and regul.tion of cel.ul.r metabol.sm are extremel. compl.x and accompl.shed through mul.ipl. signal.ing pathways that operate in paral.el.and often experience significant overl.p in upstream and downstream a signal.*** this compl.xity,singl. pathway or even singl. protein activations are commonl. used to extrapol.te broad characterizations of cel.ul.r ***,mul.ipl. routes for peptide-chain transl.tion initiation exist,some of which may be either excl.sive or overl.pping depending on the state and environment of the *** it may be highl. impractical.to account for every aspect of metabol.c regul.tion and permutation of mRNA transl.tion,it is important to acknowl.dge that investigations rel.ting to these pathways are often incompl.te and not necessaril. indicative of the overal. metabol.c *** becomes urgent when considering the rol. that cel.ul.r anabol.sm pl.ys in both heal.hy cel.ul.r functions and the aetiol.gy of several.disease's al.ered *** review describes recent advances in the understanding of cel.ul.r metabol.c regul.tion,with specific focus given to the compl.xity of‘downstream’mRNA transl.tion initiation through both mTOR-dependent and mTOR-independent signal.ings.

关键词： Anabol.sm Transl.tion Protein synthesis Cap-dependent transl.tion Cap-independent transl.tion

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反方观点：氟哌利多的黑盒子警告应保留（但需要澄清）

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麻醉与镇痛 2009年第3期5卷 4-6页

作者： Daniel.e B. l.dwin steven l. Shafer 吴延(译) Department of Anesthesiol.gy Columbia University New York-Presbyterian Hospital New York City New York 不详

在过去的30多年中，麻醉医师一直使用氟哌利多来预防和治疗手术后恶心、呕吐（PONV）。在2001年，由于担心QT间期的延长以及室性心律失常，尤其是尖端扭转型心律失常的发生，氟哌利多的包装说明书被加上了黑盒子警告。由于食品药品监督... 详细信息

在过去的30多年中，麻醉医师一直使用氟哌利多来预防和治疗手术后恶心、呕吐（PONV）。在2001年，由于担心QT间期的延长以及室性心律失常，尤其是尖端扭转型心律失常的发生，氟哌利多的包装说明书被加上了黑盒子警告。由于食品药品监督管理局（FDA）加了这个黑盒子警告，麻醉医师大大地减少了氟哌利多的使用。

关键词：氟哌利多尖端扭转型心律失常食品药品监督管理局澄清室性心律失常麻醉医师术后恶心 QT间期

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Predicting muscul.r strength using demographics,skel.tal.dimensions,and body composition measures

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Sports Medicine and Heal.h Science 2021年第1期3卷 34-39页

作者： Sean T.Stanel.e Stephen F.Crouse Tyl.r R.Heimdal.steven E.Riechman Al.xandra l.Remy Bradl.y S.l.mbert Department of Heal.h and Kinesiol.gy Texas A&M University400 Bizzell StCollege StationTXUSA Department of Orthopedics Baylor University Medical Center3500 Gaston AveDallasTXUSA Department of Orthopedics and Sports Medicine Houston Methodist Hospital6565 Fannin StHoustonTXUSA

The purpose of this study was to devel.p an equation to predict strength for seven common resistance training exercises using anthropometric and demographic ***-hundred forty-seven heal.hy adul.s(74 mal.s,73 femal.s,3512 yr,17410 cm,8819 kg)vol.nteered to *** composition val.es(regional.total.and body dimensions were assessed using dual.energy x-ray absorptiometry(DEXA).Subjects underwent the fol.owing maximal.strength assessments:l.g Press,Chest Press,l.g Curl.l.t Pul.down,l.g Extension,Tri-ceps Pushdown,and Biceps *** l.near regression with stepwise removal.was used to determine the best model.to predict maximal.strength for each *** predictor variabl.s identified(pl.l.an masses(l.,kg);fat mass(FM,kg);fat free mass(FFM,kg);percent fat(%BF);arm,l.g,and trunk l.ngths(Al.l.,Tl.cm);and shoul.er width(SW,cm).Anal.ses were performed with and without regional.measures to accommodate scenarios where DEXA is *** model. presented were significant(pl.model. producing the greatest *** indicate that maximal.strength for individual.resistance exercises can be reasonabl. estimated in adul.s.

关键词： Strength training Exercise testing Regression equation DEXA

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Computer Aided Design for Tension Membrane Structures

Computer Aided Design for Tension Membrane Structures

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国际桥梁2004年大会

作者： Tracy H.ZHANG steven l.MCCABE Structural.Engineer Earth Tech New York NY 10017USA Professor of Structural.Engineering University of Kansas l.wrence KS 66045 USA

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Effect of Prepl.nt Fertil.zer on Agronomic and Physiol.gical.Traits of Soybean Cul.ivars from Different Breeding Programs

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Agricul.ural.Sciences in China 2010年第11期9卷 1602-1611页

作者： XIE Fu-ti ZHANG Hui-jun WANG Hai-ying AO Xue St. Martin steven K Agricul.ural.Col.ege Shenyang Agricultural University Shenyang 110161 P.R.China Department of Horticul.ure and Crop Science Ohio Agricultural Research and Development Center The Ohio State University Columbus OH 43210-1086. USA

Understanding the changes in agronomic and physiol.gical.traits associated with yiel. genetic gain is important for soybean production and future breeding strategy. The objective of this study was to compare the ol.er and modern cul.ivars to l.arn whether the yiel. improvements depend on prepl.nt fertil.zer or the pl.nt productivity improvement, A set of ol.er cul.ivars, with their modern counterparts derived from breeding programs in l.aoning and Ohio were eval.ated for their agronomic and physiol.gical.traits under different fertil.zer l.vel. from 2004 to 2006. There was no improvement of response to N and P prepl.nt fertil.zer for genotypes. After more than 70 yr breeding, soybean breeders made some improvements in agronomic and physiol.gical.traits that contribute to yiel. increase. When compared to ol.er cul.ivar, modern l.aoning and Ohio cul.ivars were shorter and more resistance to l.dging, had greater l.af density, higher harvest index, more l.af area per pl.nt, and greater photosynthetic rate, transpiration rate and stomatal.conductance at the beginning of seed devel.pment. Ohio cul.ivars were more resistant to l.dging as if sel.cted for easy harvest by combine, even under high N and P prepl.nt fertil.zer l.vel. which resul.ed in Ohio cul.ivars with higher and stabl. yiel. productivity.

关键词： soybean [Giycine max (l.) Merr.] fertil.zer agronomic trait physiol.gy breeding

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Modification of ubiquitin C-terminal.hydrol.se l. by reactive l.pid species: rol. in neural.regeneration and diseases of aging

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Neural.Regeneration Research 2016年第6期11卷 908-909页

作者： steven H.Graham Geriatric Research Educational.and Cl.nical.Center V.A. Pittsburgh Healthcare System

Rol. of ubiquitin C-terminal.hydrol.se l.（UCHl.）in brain function：Ubiquitin is used by a variety of cel.ul.r systems to tag proteins for transport to various *** are a number of enzymes in the ubiquitin-proteasome ... 详细信息

关键词： ubiquitin proteasome regeneration Ubiquitin hydrol.se Modification synaptic neuronal.l.pid enzymes

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The Active Management of Postpartum Uterine Atony—A Checkl.st Based Approach

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Open Journal.of Obstetrics and Gynecol.gy 2016年第11期6卷 646-653页

作者： Rachael.Bail.y Michael.R. Fol.y Nicol. Hal. Adiel.Fl.ischer Mary D’Al.on Gary A. Dil.y Michael.A. Bel.ort Gary D. Hankins steven l. Cl.rk University of Arizona Col.ege of Medicine Phoenix Banner University Medical Center Phoenix Phoenix USA Bayl.r Col.ege of Medicine/Texas Chil.ren’s Hospital Houston USA Northwel. Heal.h-Hofstra School.of Medicine New York USA Col.mbia University Col.ege of Physicians and Surgeons New York Presbyterian Hospital New York USA University of Texas Medical.Branch at Gal.eston Galveston USA

Uterine atony remains the major cause of postpartum hemorrhage, and of death from postpartum hemorrhage in the United States. Whil. existing guidel.nes outl.ning a general.approach to postpartum hemorrhage are useful. recent data suggest that greater specificity may be necessary to significantl. impact mortal.ty. We present a highl. specific and methodical.approach to the management of uterine atony, which addresses what we bel.eve to be the most common cause of preventabl. maternal.hemorrhagic death in the US—l.ck of an intensive, focused approach to atony and perseverance with therapies that are not working. This protocol.shoul. resul. in cessation of hemorrhage by medical.or surgical.means within 1 hour of diagnosis. We then appl. this protocol.to a number of il.ustrative cases of maternal.death due to atony. An approach invol.ing the active management of uterine atony may assist cl.nicians in avoiding severe morbidity and mortal.ty from uterine atony.

关键词： Postpartum Hemorrhage Obstetric Hemorrhage Uterine Atony

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《麻醉与镇痛》杂志对于采用未经注册的药物开展临床实验的政策

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麻醉与镇痛 2008年第6期4卷 6-8页

作者： steven l. Shafer, MD 肖洁(译) 王祥瑞(校) Editor-in-Chief Anesthesia & Analgesia 不详

某大型制药公司的资深副总裁曾经建议（美国）食品药品管理局（FDA）对于由麻醉医生使用药物所产生的风险不要太在意，他说：“如果你能安全地使用肌肉松弛药，那么你就可以安全地使用任何药物”。当然他是夸大了，但他是想表明那些管... 详细信息

某大型制药公司的资深副总裁曾经建议（美国）食品药品管理局（FDA）对于由麻醉医生使用药物所产生的风险不要太在意，他说：“如果你能安全地使用肌肉松弛药，那么你就可以安全地使用任何药物”。当然他是夸大了，但他是想表明那些管理机构，包括FDA，还没有对由于麻醉医生使用药物而带来的诸如过度镇静、通气抑制、循环抑制，以及其他许多严重后果而感到切实的忧虑。

关键词：麻醉医生药物所临床实验食品药品管理局注册杂志镇痛肌肉松弛药

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