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Simultaneous harvesting and cell disruption of microalgae using ozone bubbles:optimization and characterization study for biodiesel production

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Frontiers of Chemical Science and Engineering 2021年第5期15卷 1257-1268页

作者： Wan N.A.Kadir Man K.lam Yoshimitsu Uemura Jun W.lim Peck l.Kiew steven lim Siti S.Rosli Chung Y.Wong Pau l.Show Keat T.lee Chemical Engineering Department Universiti Teknologi PETRONASSeri Iskandar32610Malaysia HICoE-Centre for Biofuel and Biochemical Research Institute of Self-Sustainable BuildingUniversiti Teknologi PETRONASSeri Iskandar32610Malaysia NPO Kuramae Bioenergy Tokyo108-0023Japan Fundamental and Applied Sciences Department Universiti Teknologi PETRONASSeri Iskandar32610Malaysia Faculty of Engineering Technology&Built EnvironmentUniversity College Sedaya InternationalCheras Kuala Lumpur56000Malaysia Department of Chemical Engineering Lee Kong Chian Faculty of Engineering and ScienceUniversiti Tunku Abdul RahmanSungai LongSelangor43000Malaysia Department of Chemical and Environmental Engineering Faculty of Science and EngineeringUniversity of Nottingham MalaysiaSemenyih43500Malaysia School of Chemical Engineering Universiti Sains MalaysiaEngineering CampusNibong Tebal14300Malaysia

In the present study, ozone was introduced as an alternative approach to harvest and disrupt microalgae cells (Chlorella vulgaris) simultaneously for biodiesel production. At the optimum ozonation conditions (6.14 g·h^(−1) ozone concentration, 30 min ozonation time, 1 l·min^(−1) of ozone flowrate at medium pH of 10 and temperature of 30℃), the sedimentation efficiency of microalgae cells increased significantly from 12.56% to 68.62%. It was observed that the microalgae cells aggregated to form flocs after pretreated with ozone due to the increment of surface charge from −20 to −6.59 mV. Besides, ozone had successfully disrupted the microalgae cells and resulted in efficient lipid extraction, which was 1.9 times higher than the control sample. The extracted microalgae lipid was mainly consisted of methyl palmitate (C16:0), methyl oleate (C18:1) and methyl linolenate (C18:3), making it suitable for biodiesel production. Finally, utilization of recycled culture media after ozonation pre-treatment showed robust growth of microalgae, in which the biomass yield was maintained in the range of 0.796 to 0.879 g ·h^(−1) for 5 cycles of cultivation.

关键词： microalgae biodiesel ozonation pre-treatment lipid

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Super-hydrophobic Surface for Skin Friction Drag Reduction in High Reynolds Number Turbulent Flow

Super-hydrophobic Surface for Skin Friction Drag Reduction i...

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第十届全国流体力学学术会议

作者： steven l Ceccio University of Michigan

Since Onda et al.(1996) reported the development of super-hydrophobic surfaces(SHS) with a fractal topology,there has been significant world-wide activity in research related to the physics,material science,and manufacture of a wide variety of SHS’*** undergoing laminar flow over SHS’s may develop a Cassie-Baxter state at the flow boundary,and the presence of the gas pockets between rough surface asperities leads to a reduction in the shear stress compared to that of a smooth solid boundary,especially in flow channels where the slip length is on the order of the channel dimension(***-fluidic devices).Yet,SHS’s influence on fully developed turbulent boundary layers(TBl) is *** order to develop SHS’s that will reduce skin friction drag at high Reynolds numbers(Re>10),our multi-university research group conducted a five-year effort focusing on five areas:(1) creation of optimized SHS’s for high Reynolds numberWe will develop methodologies to manufacture specific SHS’s with the desired surface energy,topology for the experimental examination of TBl modification at high Reynolds numbers;(2) examination of the interaction of near-wall flows with micro-scale surface gas pockets;(3) examination of the turbulence modification of micro-structured SHS’s by TBl flow;(4) development of SHSs for passive and active gas replenishment to develop and maintain the Cassie-Baxter condition;and(5) experimental validation of SHS drag reduction at high Reynolds *** talk will present an overall summary of the efforts results and conclusions.

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Acute liver injury induces expression of FGF23 in hepatocytes via orphan nuclear receptor ERRγ signaling

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Genes & Diseases 2023年第3期10卷 679-682页

作者： Yoon Seok Jung Yong-Hoon Kim Kamalakannan Radhakrishnan Jung-Ran Noh Jung Hyeon Choi Hyo-Jin Kim Jae-Ho Jeong steven Dooley Chul-Ho lee Hueng-Sik Choi School of Biological Sciences and Technology Chonnam National UniversityGwangju 61186Republic of Korea laboratory Animal Resource Center Korea Research Institute of Bioscience and Biotechnology125 Gwahak-roYuseong-guDaejeon 34141Republic of Korea Department of Functional Genomics KRIBB School of BioscienceKorea University of Science and Technology(UST)Daejeon 34113Republic of Korea Combinatorial Tumor Immunotherapy MRC Chonnam National University Medical SchoolHwasun-gunJeonnam 58128Republic of Korea Department of Microbiology Chonnam National University Medical SchoolGwangju 61186Republic of Korea Molecular Hepatology Section Medical Faculty MannheimHeidelberg University69117 HeidelbergGermany

Fibroblast growth factor 23(FGF23)is an osteocyte-and osteoblast-derived hormone that primarily regulates phosphate and vitamin D *** FGF23 levels are abnormally increased in pathological conditions like acute or chronic kidney injury,resulting in disease progression as well as increased rates of morbidity and mortality.^(1) However,FGF23 production in acute liver injury is not fully *** this study.

关键词： FGF23 acute vitamin

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Glyoxylate Reductase Isoform 1 is localized in the Cytosol and Not Peroxisomes in Plant Cells

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Journal of Integrative Plant Biology 2012年第3期54卷 152-168页

作者： steven l. K. Ching Satinder K. Gidda Amanda Rochon Owen R. van Cauwenberghe Barry J. Shelp Robert T. Mullen Department of Molecular and Cellular Biology University of Guelph Guelph Ontario N1G 2W1 Canada Department of Plant Agriculture University of Guelph Guelph Ontario N1G 2W1 Canada

Glyoxylate reductase （GlYR） is a key enzyme in plant metabolism which catalyzes the detoxification of both photorespiratory glyoxylate and succinic semialdehdye, an intermediate of the γ-aminobutyrate （GABA） pathway. Two isoforms of GlYR exist in plants, GlYR1 and GlYR2, and while GlYR2 is known to be localized in plastids, GlYR1 has been reported to be localized in either peroxisomes or the cytosol. Here, we reappraised the intracellular localization of GlYR1 in Arabidopsis thaliana l. Heynh （ecotype lansberg erecta） using both transiently-transformed suspension cells and stably-transformed plants, in combination with fluorescence microscopy. The results indicate that GlYR1 is localized exclusively to the cytosol regardless of the species, tissue and/or cell type, or exposure of plants to environmental stresses that would increase flux through the GABA pathway. Moreover, the C-terminal tripeptide sequence of GlYR1, -SRE, despite its resemblance to a type 1 peroxisomal targeting signal, is not sufficient for targeting to peroxisomes. Collectively, these results define the cytosol as the intracellular location of GlYR1 and provide not only important insight to the metabolic roles of GlYR1 and the compartmentation of the GABA and photorespiratory pathways in plant cells, but also serve as a useful reference for future studies of proteins proposed to be localized to peroxisomes and/or the cytosol.

关键词： Arabidopsis GABA GlYR1 cytosol photobleaching peroxisome PTS stress tobacco BY-2.

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Breeding high-yield superior quality hybrid super rice by rational design

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National Science Review 2016年第3期3卷 283-294页

作者： Qian Qian longbiao Guo steven M.Smith Jiayang li State Key laboratory for Rice Biology China National Rice Research InstituteChinese Academy of Agricultural Sciences State Key laboratory of Plant Genomics and National Center for Plant Gene Research(Beijing) Institute of Genetics and Developmental BiologyChinese Academy of Sciences Agricultural Genomics Institute at Shenzhen Chinese Academy of Agricultural Sciences School of Biological Sciences University of Tasmania

The challenge of meeting the increasing demand for worldwide rice production has driven a sustained quest for advances in rice breeding for yield. Two breakthroughs that led to quantum leaps in productivity last century were the introduction of semidwarf varieties and of hybrid rice. Subsequent gains in yield have been incremental. The next major leap in rice breeding is now upon us through the application of rational design to create defined ideotypes. The exploitation of wide-cross compatibility and intersubspecific heterosis,combined with rapid genome sequencing and the molecular identification of genes for major yield and quality traits have now unlocked the potential for rational design.

关键词： Oryza sativa l super rice hybrids molecular breeding grain yield grain quality

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Refinement of saliva microRNA biomarkers for sports-related concussion

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Journal of Sport and Health Science 2023年第3期12卷 369-378页

作者： steven D.Hicks Cayce Onks Raymond Y.Kim Kevin J.Zhen Jayson loeffert Andrea C.loeffert Robert P.Olympia Gregory Fedorchak Samantha DeVita Zofia Gagnon Callan Mcloughlin Miguel M.Madeira Scott l.Zuckerman Timothy lee Matthew Heller Chuck Monteith Thomas R.Campbell Christopher Neville Elise Fengler Michael N.Dretsch Department of Pediatrics College of MedicinePennsylvania State UniversityHersheyPA 17033USA Department of Orthopaedics and Rehabilitation College of MedicinePennsylvania State UniversityHersheyPA 17033USA Department of Family Medicine College of MedicinePennsylvania State UniversityHersheyPA 17033USA Department of Emergency Medicine College of MedicinePennsylvania State UniversityHersheyPA 17033USA Department of Research and Development Quadrant Biosciences Inc.SyracuseNY 13210USA Department of Biology Marist CollegePoughkeepsieNY 12601USA Sports Concussion Center College of MedicineVanderbilt UniversityNashvilleTN 37212USA Department of Family Medicine College of Osteopathic MedicineNew York Institute of TechnologyOld WestburyNY 11568USA Department of Athletic Training Colgate UniversityHamiltonNY 13346USA Department of Rehabilitation Sciences Old Dominion UniversityNorfolkVA 23529USA Department of Physical Therapy Education Orthopedicsand NeuroscienceCollege of MedicineSUNY Upstate Medical UniversitySyracuseNY 13210USA Department of Exercise Science Syracuse UniversitySyracuseNY 13210USA Department of Medical Research—West Walter Reed Army Institute of ResearchUS Army Joint Base Lewis-McChordHillhurstWA 98433USA

Background:Recognizing sport-related concussion(SRC)is challenging and relies heavily on subjective symptom *** objective,biological marker could improve recognition and understanding of *** is emerging evidence that salivary micro-ribonucleic acids(miRNAs)may serve as biomarkers of concussion;however,it remains unclear whether concussion-related miRNAs are impacted by *** sought to determine whether40 miRNAs previously implicated in concussion pathophysiology were affected by participation in a variety of contact and non-contact *** goal was to refine a miRNA-based tool capable of identifying athletes with SRC without the confounding effects of ***:This case-control study harmonized data from concussed and non-concussed athletes recruited across 10 *** of salivary miRNAs within 455 samples from 314 individuals were measured with RNA ***-subjects testing was used to identify and exclude miRNAs that changed with either(a)a single episode of exercise(166 samples from 83 individuals)or(b)season-long participation in contact sports(212 samples from 106 individuals).The miRNAs that were not impacted by exercise were interrogated for SRC diagnostic utility using logistic regression(172 samples from 75 concussed and 97 non-concussed individuals).Results:Two miRNAs(miR-532-5p and miR-182-5p)decreased(adjusted ple episode of exercise,and 1 miRNA(miR-4510)increased only after contact sports ***-three miRNAs changed at the end of a contact sports *** of these miRNAs(miR-26b-3p and miR-29c-3p)were associated(R>0.50;adjusted ple football *** the 15 miRNAs not confounded by exercise or season-long contact sports participation,11 demonstrated a significant difference(adjusted played moderate ability(area under curve>0.70)to identify concussion.A

关键词： Biomarker Contact sports Football RNA Traumatic brain injury

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An investigation of roof runoff during rain events at the Royal Military College of Canada and potential discharge to lake Ontario

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Journal of Environmental Sciences 2011年第7期23卷 1072-1078页

作者： David G. Kelly Ron D. Weir steven D. White Analytical Sciences Group & SlOWPOKE-2 Facility at RMC Department of Chemistry and Chemical Engineering Royal Military College of Canada 11 General Crerar Crescent

The Royal Military College of Canada, located on the north eastern shore of lake Ontario, possesses an abundance of copper roofs and lacks surface water treatment prior to discharge into lake Ontario. Rainwater, roof runoff and soil samples were collected and analyzed for copper and other parameters. Copper was consistently detected in runoff samples with average concentrations of 3200 ± 2100 μg/l. Multivariable linear regression analysis for a dependant copper runoff concentration yielded an adjusted R 2 value of 0.611, based on an independent variable model using minimum temperature, maximum temperature, total precipitation, and wind speed. lake water samples taken in the vicinity of storm water outfalls draining areas with copper roofs ranged from 2.0 to 40 μg/l copper. Such data exceed the 2.0 μg/l Canadian Water Quality Guidelines for the Protection of Aquatic life as outlined by the Canadian Council of Ministers of the Environment （CCME）. Analysis of raw, filtered and digested forms suggested that the majority of copper present in runoff and lake water samples was in a dissolved form. The majority of soils taken in this study displayed copper concentrations below the 63 μg/g CCME residential/parkland land use limits. These findings suggested that ion exchange processes between runoff water and soil do not occur to a sufficient extent to elevate copper levels in soil. It may therefore be concluded that the eventual fate of copper, which is not discharged via storm water outfalls, is lost to the water table and lake Ontario through the sub-soil.

关键词： copper roof runoff lake Ontario Canadian water quality guidelines for the protection of aquatic life

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“悟出的结论令我震惊……”(英文)

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菌物学报 2013年第3期32卷 321-329页

作者： VASIlYEVA larissa STEPHENSON steven l. Institute of Biology and Soil Science Far East Branch of the Russian Academy of Sciences Department of Biological Sciences University of Arkansas

阐述了对真菌经典分类与分子系统学研究的看法。

关键词：分类系统树

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Templated synthesis of crystalline mesoporous CeO_(2) with organosilane-containing polymers: balancing porosity, crystallinity and catalytic activity

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Materials Futures 2022年第2期1卷 163-173页

作者： Qiang luo Zichao Wei Hanyi Duan lei Jin Rumasha N T Kankanamage Seth Shuster steven l Suib James F Rusling Jie He Department of Chemistry University of ConnecticutStorrsCT 06269United States of America Polymer Program University of ConnecticutStorrsCT 06269United States of America Institute of Materials Science University of ConnecticutStorrsCT 06269United States of America Department of Surgery and Neag Cancer Center UConn HealthFarmingtonCT 06232United States of America School of Chemistry National University of Ireland GalwayGalwayIreland

We report the synthesis of ordered mesoporous ceria(mCeO_(2))with highly crystallinity and thermal stability using hybrid polymer templates consisting of *** organosilane-containing polymers can convert into silica-like nanostructures that further serve as thermally stable and mechanically strong templates to prevent the collapse of mesoporous frameworks during thermal-induced *** a simple evaporation-induced self-assembly process,control of the interaction between templates and metal precursors allows the co-self-assembly of polymer micelles and Ce^(3+)ions to form uniform porous *** porosity is well-retained after calcination up to 900℃.After the thermal engineering at 700℃ for 12 h(mCeO_(2)-700-12 h),mCeO_(2) still has a specific surface area of 96 m^(2) g^(−1) with a pore size of 14 ***_(2) is demonstrated to be active for electrochemical oxidation of ***_(2)-700-12 h with a perfect balance of crystallinity and porosity shows the fastest intrinsic activity that is about 84 times more active than bulk CeO_(2) and 5 times more active than mCeO_(2) that has a lower crystallinity.

关键词： mesoporous materials cerium oxides crystalline oxides electrocatalysis sulfite oxidation

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Comparative assessment on rodent impacts and cultural perceptions of ecologically based rodent management in 3 Afro-Malagasy farming regions

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Integrative Zoology 2020年第6期15卷 578-594页

作者： Natasha l.CONSTANT lourens H.SWANEPOEl Samual T.WIllIAMS Voahangy SOARIMAlAlA steven M.GOODMAN Apia T.MASSAWE loth S.MUlUNGU Rhodes H.MAKUNDI Mashaka E.MDANGI Peter J.TAYlOR steven R.BElMAIN SARChI Chair on Biodiversity Value and Change and Core Team Member of the Centre for Invasion Biology School of Mathematical and Natural SciencesUniversity of VendaThohoyandouJohannesburgSouth Africa Sustainable Places Research Institute Cardiff UniversityCardiffUK Department of Zoology School of Mathematical and Natural SciencesUniversity of VendaThohoyandouSouth Africa Department of Anthropology Durham UniversityDurhamUK Institute for Globally Distributed Open Research and Education(IGDORE) JohannesburgSouth Africa Association Vahatra AntananarivoMadagascar Institut des Sciences et Techniques de l’EnvironnementUniversitéde Fianarantsoa FianarantsoaMadagascar Field Museum of Natural History ChicagoIllinoisUSA Pest Management Centre Sokoine University of AgricultureMorogoroTanzania Ministry of Agriculture Tanzania Natural Resources InstituteUniversity of Greenwich KentUK

Rodents generate negative consequences for smallholder farmers in Africa that directly impact household and livestock damage,food security,and public *** Based Rodent Management(EBRM)seeks sustainable solutions for the mitigation of rodent damage through assessments of rodent population dynamics,agro-ecosystems,and socio-cultural *** adopt a comparative approach across 3 rural Afro-Malagasy smallholder farming regions in South Africa,Tanzania,and Madagascar to assess the household impacts of rodent pests and current perceptions and preferences associated with several rodent control *** conducted focus group questionnaires and interviews in different study site *** assert multiple impacts on Afro-Malagasy farmers demonstrating recurrent and emerging agricultural and household costs,and public health *** identify a significant knowledge gap in educating communities about the application of different EBRM approaches in favor of acute poisons that are perceived to be more *** issues and taboos also have a significant impact on the social acceptance of rodent hunting as well as biological control using indigenous *** advocate for an enhanced investigation of the socio-cultural beliefs associated with different rodent practices to understand the factors underlying social acceptance.A collaborative approach that integrates the perspectives of target communities to inform the design of EBRM initiatives according to the specific agro-ecosystem and socio-cultural context is necessary to ensure programmatic success.

关键词： Africa beliefs crop damage ecologically based rodent management farmer survey

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