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World Journal of Gastroenterology 2006年第32期12卷 5182-5185页

作者： stephanos karagiannis Spyros Goulas Georgios Kosmadakis Petros Galanis Dimitrios Arvanitis John Boletis Evangelos Georgiou Christos Mavrogiannis Academic Department of Gastroenterology Faculty of Nursing Athens UniversityGeneral Hospital of Athens "Helena Venizelou"Greece Department of Nephrology and Transplantation Center Laiko General Hospital AthensGreece Department of Nephrology A. Fleming General Hospital AthensGreece Medical Physics Department Medical School Athens UniversityGreece

瞄准：在小肠(SB ) 的察觉调查无线的囊内视镜检查法(WCE ) 的角色在有长期的肾衰竭(CRF ) 并且阴暗流血的病人的病理。方法：有阴暗流血的连续 CRF 病人有希望地被学习。有正常肾的功能并且阴暗流血的病人，与 WCE 在一样的时期期间调... 详细信息

瞄准：在小肠(SB ) 的察觉调查无线的囊内视镜检查法(WCE ) 的角色在有长期的肾衰竭(CRF ) 并且阴暗流血的病人的病理。方法：有阴暗流血的连续 CRF 病人有希望地被学习。有正常肾的功能并且阴暗流血的病人，与 WCE 在一样的时期期间调查了，被用于结果的解释。结果：十七个 CRF 病人(11 公开， 6 玄术流血) 并且 51 个病人(33 公开， 18 玄术流血) 与正常，肾的功能在这研究被注册。积极 SB 调查结果在 70.6% CRF 病人被检测(P < 0.05 ) 。SB angiodysplasia 在 47% CRF 病人被识别。Univariate 逻辑回归为 angiodysplasia 作为一个重要预兆的因素揭示了 CRF (P < 0.05 ) 。治疗学的措施与积极调查结果在 66% 病人被承担。结论：根据我们的初步的结果， SB angiodysplasia 与阴暗流血在 CRF 病人之中在增加的流行被发现。WCE 在胃肠的病理的诊断并且在计划适当治疗学的干预是有用的并且因此，应该在这组病人的病情的检查被包括。

关键词：内窥镜慢性肾疾病肾功能胃疾病肠疾病

EZH2 inhibitors promote β-like cell regeneration in young and adult type 1 diabetes donors

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Signal Transduction and Targeted Therapy 2024年第2期9卷 679-692页

作者： Keith Al-Hasani Safiya Naina Marikar Harikrishnan Kaipananickal Scott Maxwell Jun Okabe Ishant Khurana Thomas karagiannis Julia J.Liang Lina Mariana Thomas Loudovaris Thomas Kay Assam El-Osta Epigenetics in Human Health and Disease Program Baker Heart and Diabetes Institute75 Commercial RoadMelbourne 3004 VICAustralia Department of Diabetes Central Clinical SchoolMonash UniversityMelbourne 3004 VICAustralia Epigenetics in Human Health and Disease Laboratory Central Clinical SchoolMonash UniversityMelbourne 3004 VICAustralia School of Science STEM CollegeRMIT UniversityMelbourne 3001 VICAustralia Immunology and Diabetes Unit St Vincent’s Institute of Medical ResearchFitzroy 3065 VICAustralia Department of Medicine and Therapeutics The Chinese University of Hong KongSha TinHong Kong SAR Hong Kong Institute of Diabetes and Obesity Prince of Wales HospitalThe Chinese University of Hong Kong3/F Lui Che Woo Clinical Sciences Building30-32-Ngan Shing StreetSha TinHong Kong SAR Li Ka Shing Institute of Health Sciences The Chinese University of Hong KongSha TinHong Kong SAR Biomedical Laboratory Science Department of TechnologyFaculty of HealthUniversity College CopenhagenCopenhagenDenmark

β-cells are a type of endocrine cell found in pancreatic islets that synthesize,store and release *** type 1 diabetes(T1D),T-cells of the immune system selectively destroy the insulin-producingβ-*** of these cells leads to a lifelong dependence on exogenous insulin administration for ***,there is an urgent need to identify novel therapies that stimulateβ-cell growth and induceβ-cell *** and others have shown that pancreatic ductal progenitor cells are a promising source for regeneratingβ-cells for T1D owing to their inherent differentiation *** transcriptional suppression is refractory to exocrine reaction and tightly controls the regenerative potential by the EZH2 *** the present study,we show that transient stimulation of exocrine cells,derived from juvenile and adult T1D donors to the FDAapproved EZH2 inhibitors GSK126 and Tazemetostat(Taz)influence a phenotypic shift towards aβ-like cell *** transition from repressed to permissive chromatin states are dependent on bivalent H3K27me3 and H3K4me3 chromatin *** EZH2 is fundamental toβ-cell regenerative *** pancreatic ductal cells exhibit insulin production and secretion in response to a physiological glucose challenge ex *** pre-clinical studies underscore the potential of small molecule inhibitors as novel modulators of ductal progenitor differentiation and a promising new approach for the restoration ofβ-like cell function.

关键词： EZH2 struction inhibitors

Impaired ketogenesis ties metabolism to T cell dysfunction in COVID-19

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四川生理科学杂志 2022年第7期44卷 1271-1271页

作者： Fotios karagiannis Immunopathology Unit Institute of Clinical Chemistry and Clinical Pharmacology Medical Faculty University Hospital Bonn University of Bonn Bonn Germany Department of Anesthesiology and Intensive Care Medicine University Hospital Bonn Venusberg-Campus 1 Bonn Germany Department of Bioinformatics and Biochemistry Braunschweig Integrated Center of Systems Biology Technische Universität Braunschweig Brunswick Germany Immunology and Metabolism Unit Life & Medical Sciences Institute (LIMES) University of Bonn Bonn Germany Department of Internal Medicine and Radboud Center for Infectious Diseases Radboud University Medical Center Nijmegen the Netherlands Department of Respiratory Medicine and German Centre of Lung Research (DZL) Hannover Medical School Carl-Neuberg-Str. 1 Hannover Germany Institute of Intensive Care Medicine University Hospital Zurich Zurich Switzerland Department of Intensive Care Medicine and Radboud Center for Infectious Diseases Radboud University Medical Center Nijmegen the Netherlands Department of Anesthesiology and Intensive Care Medicine University Hospital Essen University Duisburg-Essen Essen Germany Institute of Experimental Immunology Medical Faculty University Hospital Bonn University of Bonn Bonn Germany Institute of Innate Immunity Medical Faculty University Hospital Bonn University of Bonn Bonn Germany Institute of Clinical Chemistry and Clinical Pharmacology Medical Faculty University Hospital Bonn University of Bonn Bonn Germany Institute of Virology Medical Faculty University Hospital Bonn University of Bonn Bonn Germany Institute of Cardiovascular Immunology University Hospital Bonn University of Bonn Bonn Germany

Anorexia and fasting are host adaptations to acute infection, inducing a metabolic switch towards ketogenesis and the production of ketone bodies, including β-hydroxybutyrate (BHB) 1-6. However, whether ketogenesis metabolically influences the immune response in pulmonary infections remains unclear. Here we report impaired production of BHB in humans with SARS-CoV-2-induced but not influenza-induced acute respiratory distress syndrome (ARDS). CD4^(+) T cell function is impaired in COVID-19 and BHB promotes both survival and production of Interferon-γ from CD4^(+) T cells. Using metabolic tracing analysis, we uncovered that BHB provides an alternative carbon source to fuel oxidative phosphorylation (OXPHOS) and the production of bioenergetic amino acids and glutathione, which is important for maintaining the redox balance. T cells from patients with SARS-CoV-2-induced ARDS were exhausted and skewed towards glycolysis, but can be metabolically reprogrammed by BHB to perform OXPHOS, thereby increasing their functionality.

关键词： impaired metabolism thereby

Role of gap junctions in endothelium-derived hyperpolarizing factor-mediated vasodilatation in rat renal artery

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Acta Pharmacologica Sinica 2004年第8期 57-63页

作者： Joanna karagiannis,Michael RAND Division of Biosciences Division of Biosciences School of Medical Sciences RMIT University Bundoora Victoria 3083 Australia School of Medical Sciences RMIT University Bundoora Victoria 3083 Australia

AIM: To investigate the effect of cyclin-dependent kinase 5 (Cdk5) inhibitor roscovitine on the morphine antinociceptive tolerance development in rats. METHODS: Tail-flick test as pain threshold measurement and intrathecal injection techniques were used. RESULTS: Intrathecal roscovitine infusion alone produced an antinociceptive effect. Tolerance was induced by continuous intrathecal infusion of morphine 5μg/h for 5 d. Co administration of intrathecal roscovitine 1μg/h for 5 d enhanced the morphine antinociceptive effect in tolerant rats. It also caused a shift in the morphine antinociceptive dose"Cresponse curve to the left when co-administered with morphine during tolerance induction, and caused a 67 % reduction in the increase in the ED50 of morphine (dose producing 50 % of the maximum response). CONCLUSION: Cdk5 modulation is involved in the antinociceptive tolerance of morphine. Intrathecal roscovitine administration could attenuate this tolerance development.

关键词： carbachol endothelium-derived hyperpolarizing factor gap junction gap 27 peptide renal artery

SNVDis:A Proteome-wide Analysis Service for Evaluating nsSNVs in Protein Functional Sites and Pathways

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Genomics, Proteomics & Bioinformatics 2013年第2期11卷 122-126页

作者： Konstantinos karagiannis Vahan Simonyan Raja Mazumder Department of Biochemistry and Molecular Biology George Washington University Medical Center Center for Biologics Evaluation and Research Food and Drug Administration

Amino acid changes due to non-synonymous variation are included as annotations for individual proteins in UniProtKB/Swiss-Prot and RefSeq which present biological data in a pro- tein- or gene-centric fashion. Unfortunately, proteome-wide analysis of non-synonymous single- nucleotide variations （nsSNVs） is not easy to perform because information on nsSNVs and func- tionally important sites are not well integrated both within and between databases and their search engines. We have developed SNVDis that allows evaluation of proteome-wide nsSNV distribution in functional sites, domains and pathways. More specifically, we have integrated human-specific data from major variation databases （UniProtKB, dbSNP and COSMIC）, comprehensive sequence feature annotation from UniProtKB, Pfam, RefSeq, Conserved Domain Database （CDD） and pathway information from Protein ANalysis THrough Evolutionary Relationships （PANTHER） and mapped all of them in a uniform and comprehensive way to the human reference proteome pro- vided by UniProtKB/Swiss-Prot. Integrated information of active sites, pathways, binding sites, domains, which are extracted from a number of different sources, provides a detailed overview of how nsSNVs are distributed over the human proteome and pathways and how they intersect with functional sites of proteins. Additionally, it is possible to find out whether there is an over- or under-representation of nsSNVs in specific domains, pathways or user-defined protein lists. The underlying datasets are updated once every 3 months. SNVDis is freely available at http：//***- ***/tool/snvdis.

关键词： Active site Binding site N-linked glycosylation nsSNP nsSNV Variation

Clinical presentation and outcomes of chronic dialysis patients with COVID-19:A single center experience from Greece

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World Journal of Nephrology 2022年第2期11卷 58-72页

作者： Dimitra Bacharaki Minas karagiannis Aggeliki Sardeli Panagiotis Giannakopoulos Nikolaos Renatos Tziolos Vasiliki Zoi Nikitas Piliouras Nikolaos-Achilleas Arkoudis Nikolaos Oikonomopoulos Kimon Tzannis Dimitra Kavatha Anastasia Antoniadou Demetrios Vlahakos Sophia Lionaki Nephrology Unit Department of Internal Medicine"Attikon"University HospitalChaidari 12462Greece Medical School National and Kapodistrian University of AthensAthens 15772Greece Department of Internal Medicine "Attikon"University HospitalChaidari 12462Greece Department of Radiology "Attikon"University HospitalChaidari 12462Greece

BACKGROUND Coronavirus disease 2019(COVID-19)is still a menacing pandemic,especially in vulnerable *** and mortality from COVID-19 in maintenance hemodialysis(MHD)patients are considered worse than those in the general population,but vary across continents and countries in *** To describe the clinical course and outcomes of hospitalized MHD patients with COVID-19 in a retrospective observational single center study in *** We correlated clinical,laboratory,and radiological data with the clinical outcomes of MHD patients hospitalized with COVID-19 during the *** diagnosis was confirmed by real-time polymerase chain *** was determined as survivors vs non-survivors and“progressors”(those requiring oxygen supplementation because of COVID-19 pneumonia worsening)vs“non-progressors”.RESULTS We studied 32 patients(17 males),with a median age of 75.5(IQR:58.5-82)years *** those,12 were diagnosed upon screening and 20 with related *** to the World Health Organization(WHO)score,the severity on admission was mild disease in 16,moderate in 13,and severe in 3 *** computed tomography(CT)showed 1-10%infiltrates in 24 ***“progressors”were recorded among included *** case fatality rate was 5/32(15.6%).Three deaths occurred among“progressors”and two in“non-progressors”,irrespective of co-morbidities and *** of mortality on admission included frailty index,chest CT findings,WHO severity score,and thereafter the increasing values of serum LDH and D-dimers and decreasing serum *** of becoming a“progressor”included increasing number of neutrophils and neutrophils/lymphocytes *** Patients on MHD seem to be at higher risk of COVID-19 mortality,distinct from the general *** laboratory parameters on admission and during follow-up may be helpful in risk stratification and management of patients.

关键词： COVID-19 SARS-CoV-2 Dialysis Greece Clinical course Outcome

Athletes with mild post-COVID-19 symptoms experience increased respiratory and metabolic demands:А cross-sectional study

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Sports Medicine and Health Science 2023年第2期5卷 106-111页

作者： Vasileios T.Stavrou Astara Kyriaki George D.Vavougios Ioannis G.Fatouros George S.Metsios Konstantinos Kalabakas Dimitrios karagiannis Zoe Daniil Konstantinos I.Gourgoulianis George Вasdekis Laboratory of Cardio-Pulmonary Testing and Pulmonary Rehabilitation Respiratory Medicine DepartmentFaculty of MedicineUniversity of ThessalyLarissaGreece The Medical Project PreventionEvaluation and Recovery CenterLarissaGreece Department of Neurology 417 Army Equity Fund Hospital(NIMTS)AthensGreece Department of Neurology Faculty of MedicineUniversity of CyprusLefkosiaCyprus School of Physical Education and Sport Sciences University of ThessalyTrikalaGreece Department of Nutrition and Dietetics University of ThessalyTrikalaGreece

Coronavirus Disease 2019(COVID-19)has significantly affected different physiological systems,with a potentially profound effect on athletic ***,to date,such an effect has been neither addressed nor ***,the aim of this study was to investigate fitness indicators,along with the respiratory and metabolic profile,in post-COVID-19 *** male soccer players,were divided into two groups:non-hospitalized COVID-19(n=20,Age:[25.2±4.1]years,Body Surface Area[BSA]:[1.9±0.2]m^(2),body fat:11.8%±3.4%)versus[vs]healthy(n=20,Age:[25.1±4.4]years,BSA:[2.0±0.3]m^(2),body fat:10.8%±4.5%).For each athlete,prior to cardiopulmonary exercise testing(CPET),body composition,spirometry,and lactate blood levels,were *** between groups were assessed with the independent samples t-test(p<0.05).Several differences were detected between the two groups:ventilation(:Resting:[14.7±3.1]L·min^(−1)vs.[11.5±2.6]L·min^(−1),p=0.001;Maximal Effort:[137.1±15.5]L·min^(−1)vs.[109.1±18.4]L·min^(−1),p<0.001),ratio VE/maximal voluntary ventilation(Resting:7.9%±1.8%vs.5.7%±1.7%,p<0.001;Maximal Effort:73.7%±10.8%vs.63.1%±9.0%,p=0.002),ratioVE/BSA(Resting:7.9%±2.0%vs.5.9%±1.4%,p=0.001;Maximal Effort:73.7%±11.1%vs.66.2%±9.2%,p=0.026),heart rate(Maximal Effort:[191.6±7.8]bpm vs.[196.6±8.6]bpm,p=0.041),and lactate acid(Resting:[1.8±0.8]mmol·L^(−1)vs.[0.9±0.1]mmol·L^(−1),p<0.001;Maximal Effort:[11.0±1.6]mmol·L^(−1)vs.[9.8±1.2]mmol·L^(−1),p=0.009),during *** significant differences were identified regarding maximal oxygen uptake([55.7±4.4]ml·min^(−1)·kg−1 vs.[55.4±4.6]ml·min^(−1)·kg−1,p=0.831).Our findings demonstrate a pattern of compromised respiratory function in post-COVID-19 athletes characterized by increased respiratory work at both rest and maximum effort as well as hyperventilation during exercise,which may explain the reported increased metabolic needs.

关键词： Cardiopulmonary exercise testing Infected with COVID-19 Respiratory work

Ecological Consequences of Mechanization of the Wood Skidding Works in Greece

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Journal of Environmental Science and Engineering(B) 2012年第4期1卷 465-472页

作者： Evaggelos karagiannis Ploutarchos Kararizos Department of Forest Engineering and Surveying School of Forestry and Natural Environment Aristotle University of ThessalonikiThessaloniki 54124 Greece

The movement of the wood skidding means on the natural ground causes damages in the soil and in the remaining stand, the size of which depends on the kind, the resistance and the soil conditions, from the size and movements frequency on the machines as well as from the length of the skidding wood. This study was carried out in a productive forest of Greece, the resistance of the soil in connection with the moisture and the category of the native stone, the pressure that tires of tractors exert in the soil, are estimated. It also estimated the percentage of the trees in the remaining stand which suffer damages from the movement of various means. The above means are used for the skidding of wood of different lengths. According to the results of the research some measures are suggested so as to decrease the damages, which are caused in the soil and in the remaining stand.

关键词：木材生态后果希腊机械化土壤条件集材运动频率损害赔偿

Structure-based Comparative Analysis and Prediction of N-linked Glycosylation Sites in Evolutionarily Distant Eukaryotes

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Genomics, Proteomics & Bioinformatics 2013年第2期11卷 96-104页

作者： Phuc Vinh Nguyen Lam Radoslav Goldman Konstantinos karagiannis Tejas Narsule Vahan Simonyan Valerii Soika Raja Mazumder Life Sciences Department Paris Diderot University Department of Biochemistry and Molecular Biology George Washington University Medical Center Department of Oncology Georgetown University Center for Biologics Evaluation and Research Food and Drug Administration

The asparagine-X-serine/threonine （NXS/T） motif, where X is any amino acid except proline, is the consensus motif for N-linked glycosylation. Significant numbers of high-resolution crystal structures of glycosylated proteins allow us to carry out structural analysis of the N-linked glycosylation sites （NGS）. Our analysis shows that there is enough structural information from diverse glycoproteins to allow the development of rules which can be used to predict NGS. A Python-based tool was developed to investigate asparagines implicated in N-glycosylation in five species： Homo sapiens, Mus musculus, Drosophila melanogaster, Arabidopsis thaliana and Saccharo- myees cerevisiae. Our analysis shows that 78 % of all asparagines of NXS/T motif involved in N-gly- cosylation are localized in the loop/turn conformation in the human proteome. Similar distribution was revealed for all the other species examined. Comparative analysis of the occurrence of NXS/T motifs not known to be glycosylated and their reverse sequence （S/TXN） shows a similar distribu- tion across the secondary structural elements, indicating that the NXS/T motif in itself is not bio- logically relevant. Based on our analysis, we have defined rules to determine NGS. Using machine learning methods based on these rules we can predict with 93% accuracy if a particular site will be glycosylated. If structural information is not available the tool uses structural prediction results resulting in 74% accuracy. The tool was used to identify glycosylation sites in 108 human proteins with structures and 2247 proteins without structures that have acquired NXS/T site/s due to non-synonymous variation. The tool, Structure Feature Analysis Tool （SFAT）, is freely available to the public at http：//***/tools/sfat.

关键词： N-linked glycosylation Gain and loss of glycosyla-tion nsSNP nsSNV Variation

Decoding altitude-activated regulatory mechanisms occurring during apple peel ripening

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Horticulture Research 2020年第1期7卷 1149-1160页

作者： Evangelos karagiannis Michail Michailidis Georgia Tanou Federico Scossa Eirini Sarrou George Stamatakis Martina Samiotaki Stefan Martens Alisdair R.Fernie Athanassios Molassiotis Laboratory of Pomology Department of AgricultureAristotle University of Thessaloniki54124 ThessalonikiGreece Institute of Soil and Water Resources ELGO-DEMETERThermiThessaloniki 57001Greece Max-Planck-Institute of Molecular Plant Physiology Am Müehlenberg 1.Potsdam-Golm 14476Germany Council for Agricultural Research and Economics Research Center for Genomics and BioinformaticsVia Ardeatina 54600178 RomeItaly Institute of Plant Breeding and Genetic Resources ELGO-DEMETERThermiThessaloniki 57001Greece Biomedical Sciences Research Center“Alexander Fleming” Vari 16672Greece Fondazione Edmund Mach Centro Ricerca e InnovazioneDepartment of Food Quality and NutritionVia E.Mach138010 San Michele all’AdigeTNItaly

Apple(Malus domestica Borkh)is an important fruit crop cultivated in a broad range of environmental *** fruit ripening is a physiological process,whose molecular regulatory network response to different environments is still not sufficiently investigated and this is particularly true of the peel *** this study,the influence of environmental conditions associated with low(20 m)and high(750 m)altitude on peel tissue ripening was assessed by physiological measurements combined with metabolomic and proteomic analyses during apple fruit development and *** apple fruit ripening was itself not affected by the different environmental conditions,several key color parameters,such as redness and color index,were notably induced by high *** with this observation,increased levels of anthocyanin and other phenolic compounds,including cyanidin-3-O-galactoside,quercetin-3-O-rhamnoside,quercetin-3-O-rutinoside,and chlorogenic acid were identified in the peel of apple grown at high ***,the high-altitude environment was characterized by elevated abundance of various carbohydrates(e.g.,arabinose,xylose,and sucrose)but decreased levels of glutamic acid and several related proteins,such as glycine hydroxymethyltransferase and glutamate–glyoxylate *** processes affected by high altitude were the TCA cycle,the synthesis of oxidative/defense enzymes,and the accumulation of photosynthetic *** the obtained data we were able to construct a metabolite-protein network depicting the impact of altitude on peel *** combined analyses presented here provide new insights into physiological processes linking apple peel ripening with the prevailing environmental conditions.

关键词： altitude network prevailing