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Effects of tanshinone on neuropathological changes induced by amyloid β-peptide(1-40)injection in rat hippocampus

Acta Pharmacologica Sinica 2004年第7期25卷 15-22页

作者： Long-xuan LI2, Jia-pei DAI3, Li-qiang RU4, Guang-fu YIN4, Bin ZHAO Department of Neurology, Affiliated Hospital, Guangdong Medical College, Zhanjiang 524001, China 3The Netherlands Institute for Brain Research, Amsterdam 1105AZ, the Netherlands 4Department of Neurobiology, TongjiMedical College of Huazhong Universityof Science and Technology, Wuhan 430030, China Department of Neurology Affiliated Hospital Guangdong Medical College Zhanjiang 524001 The Netherlands Institute for Brain Research Zhanjiang 524001 Department of Neurobiology Tongji Medical College of Huazhong University of Science and Technology Wuhan 430030

AIM: To investigate the effect of tanshinone (Tan) on the neuropathological changes induced by amyloid β- peptide1-40(Aβ1-40) injection in hippocampus in rats. METHODS: Aβ1-4010 μg was injected bilaterally into the dorsal blade of the dentate gyrus in the hippocampus. The level of acetylcholinesterase (AChE) in hippocampus was evaluated by histochemistry. The expressions of neuronal nitric oxide synthase (nNOS) and inducible form of NOS (iNOS) were detected by immunohistochemistry and Western blot. Aβ1-40-injected rats were treated ig with Tan, the major active ingredient from Salvia miltiorrhiza of Chinese herb extract. RESULTS: The level of AChE positive fibers of each subfield in Aβ1-40-injected hippocampus decreased significantly compared with those of control (P40 injection in hippocampus.

关键词： tanshinone amyloid beta-protein acetylcholinesterase nitric oxide synthase hippocampus

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贴面在口腔修复学的应用

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中华口腔医学杂志 1991年第3期26卷 174-175页

作者： step.,hy Raym.,LB 香港牙医学会

贴面是治疗釉质损坏及牙齿严重变色的首选方法,且尤适用于青年患者。该种方法的优点如下:①外观上即时收

关键词：贴面口腔修复学

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Anticancer effect of aloe-emodin on cervical cancer cells involves G2/M arrest and induction of differentiation

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Acta Pharmacologica Sinica 2007年第12期28卷 1991-1995页

作者： Jun-ming GUO~(2,3),Bing-xiu XIAO~2,Qiong LIU~2,Shun ZHANG~(2,4),Dong-hai LIU~(2,4),Zhao-hui GONG~2~2 Ningbo University School of Medicine,Ningbo 315211,China ~4 Affiliated Hospital(Ningbo № 2 Hospital),Ningbo University School ofMedicine,Ningbo 315010,China Ningbo University School of Medicine Ningbo China Affiliated Hospital (Ningbo No 2 Hospital) Ningbo University School of Medicine Ningbo China

Aim:The aim of this study was to investigate the effects of aloe-emodin,a naturalcompound from the root and rhizome of Rheum palmatum,on the growth of hu-man cervical cancer cells,***:HeLa ceils were treated with variousconcentrations of aloe-emodin for 1-5 d,and cell growth was measured by 3-（4,5-dimethylthiazol-2-yl）-2,5-diphenyl tetrazolium bromide *** long-termgrowth effect was investigated by crystal violet *** distributions of thecell cycle and apoptosis were analyzed by flow *** alkaline phos-phatase （ALP） activity was analyzed by a chemical ***,Westernblotting was used to indicate the abundant changes of protein kinase C （PKC）,c-myc,cyclins,cyclin-dependent kinases （CDK）,and proliferating cell nuclear anti-gen （PCNA）.Results:Aloe-emodin inhibited the growth of HeLa cells in a dose-dependent manner at concentrations ranging between 2.5 and 40 μmol/*** cytometric analysis showed that HeLa cells were arrested at the G2/M *** effect was associated with the decrease in cyclin A and CDK2,and theincrease in cyclin B1 and *** importantly,the ALP activity was found tobe increased by aloe-emodin treatment,and accompanied by the inhibition ofPCNA *** addition,aloe-emodin suppressed the expression of PKCαand ***:These findings provide a possible mechanistic explana-tion for the growth inhibitory effect of aloe-emodin on HeLa,which includes cellcycle arrest and inducing differentiation.

关键词： HeLa cells cell cycle aloe-emodin alkaline phosphatase c-myc

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RNA interference by expression of short hairpin RNAs suppresses bcl-xL gene expression in nasopharyngeal carcinoma cells

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Acta Pharmacologica Sinica 2005年第2期26卷 228-234页

作者： Fang LIU~2,Cheng-wei HE,Yue-fei ZHANG~4,Ke-yuan ZHOU~3~2Institute of Biochemistry and Molecular Biology ~4Department of Otorhinolaryngology,The First Affiliated Hospital,Guangdong MedicalCollege,Zhanjiang 524023,China Institute of Biochemistry and Molecular Biology Guangdong Medical College Zhanjiang China Department of Otorhinolaryngology The First Affiliated Hospital Guangdong Medical College Zhanjiang China

Aim:To evaluate a new plasmid mediated RNA interference(RNAi)system andinvestigate whether knock-down of bcl-xL by short hairpin RNA(shRNA)caninduce apoptosis of human nasopharyngeal carcinoma(NPC)cell line CNE-2Z ***:The plasmid containing mU6 promoter was subcloned to yield thepmU6 plasmid,recombinant plasmid expressing shRNA targeting bcl-xL gene wasdesigned and constructed,and were co-transfected cells with green fluorescenceprotein expressing *** cytometry was used to evaluate transfectionefficiency,and RT-PCR and Western blot were applied to analyze bcl-xL mRNAand protein levels,***:The shRNA expressed by the recombi-nant plasmid efficiently suppressed bcl-xL gene expression and induced apoptosisof NPC cells in ***:The recombinant plasmid can sufficientlymediate RNAi in CNE-2Z cells,and knock-down of the bcl-xL expression by shRNAsignificantly induced apoptosis in CNE-2Z *** results suggest this newsystem,mediated RNAi can be used as a tool for the study of gene function andgene therapy.

关键词： RNAi plasmid gene transfection short hairpin RNA bcl-xL apoptosis nasopharyngeal carcinoma cells

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