Anticancer effect of aloe-emodin on cervical cancer cells involves G2/M arrest and induction of differentiation

作     者：Jun-ming GUO~(2,3),Bing-xiu XIAO~2,Qiong LIU~2,Shun ZHANG~(2,4),Dong-hai LIU~(2,4),Zhao-hui GONG~2~2 Ningbo University School of Medicine,Ningbo 315211,China ~4 Affiliated Hospital(Ningbo № 2 Hospital),Ningbo University School ofMedicine,Ningbo 315010,China 

作者机构：Ningbo University School of Medicine Ningbo China Affiliated Hospital (Ningbo No 2 Hospital) Ningbo University School of Medicine Ningbo China 

出 版 物：《Acta Pharmacologica Sinica》 (中国药理学报(英文版))

年 卷 期：2007年第28卷第12期

页      面：1991-1995页

核心收录：

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

基　　金：the Ningbo Natural Science Foundation (No 2006A610047) the "151 Personal Training Project" of Zhejiang Province, China the "Famous Professor Training Project" of Ningbo, China the "Key Subjects Fund of Ningbo University (No XK0614052) the KC Wong Magna Fund at Ningbo Univer-sity 

主　　题：HeLa cells cell cycle aloe-emodin alkaline phosphatase c-myc 

摘      要：Aim:The aim of this study was to investigate the effects of aloe-emodin,a naturalcompound from the root and rhizome of Rheum palmatum,on the growth of hu-man cervical cancer cells,***:HeLa ceils were treated with variousconcentrations of aloe-emodin for 1-5 d,and cell growth was measured by 3-（4,5-dimethylthiazol-2-yl）-2,5-diphenyl tetrazolium bromide *** long-termgrowth effect was investigated by crystal violet *** distributions of thecell cycle and apoptosis were analyzed by flow *** alkaline phos-phatase （ALP） activity was analyzed by a chemical ***,Westernblotting was used to indicate the abundant changes of protein kinase C （PKC）,c-myc,cyclins,cyclin-dependent kinases （CDK）,and proliferating cell nuclear anti-gen （PCNA）.Results:Aloe-emodin inhibited the growth of HeLa cells in a dose-dependent manner at concentrations ranging between 2.5 and 40 μmol/*** cytometric analysis showed that HeLa cells were arrested at the G2/M *** effect was associated with the decrease in cyclin A and CDK2,and theincrease in cyclin B1 and *** importantly,the ALP activity was found tobe increased by aloe-emodin treatment,and accompanied by the inhibition ofPCNA *** addition,aloe-emodin suppressed the expression of PKCαand ***:These findings provide a possible mechanistic explana-tion for the growth inhibitory effect of aloe-emodin on HeLa,which includes cellcycle arrest and inducing differentiation.