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Genistein stimulates osteoblastic differentiation via p38 MAPK-Cbfa1 pathway in bone marrow culture

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Acta Pharmacologica Sinica 2007年第10期28卷 1597-1602页

作者： Qing-chuan LIAO~2,Zhou-sheng XIAO~(4,5),Yan-fang QIN~3,Hong-hao ZHOU~4~2 Affiliated Children's Hospital,Nanjing Medical University,Nanjing 210008,China,~3 Department of Pathology,School of Medicine,JinanUniversity,Guangzhou 510632,China ~4 Institute of Clinical Pharmacology,Central South University,Changsha 410078,China Affiliated Children's Hospital Nanjing Medical University Nanjing China Institute of Clinical Pharmacology Central South University Changsha China Department of Pathology School of Medicine Jinan University Guangzhou China

Aim:To test the hypothesis that genistein stimulates the osteoblastic differentia-tion through the p38 mitogen activated protein kinase (MAPK)-core-binding fac-tor 1 (Cbfa1) ***:The activation of p38 MAPK was detected byWestern *** phosphatase (ALP) activity and calcium depositionwere assessed for osteoblastic differentiation of bone marrow-derived mesenchy-mal stem cell (BMSC) *** expression of Cbfa1 was analyzed at both themRNA and protein *** activity of Cbfa1 was detected by electrophoreticmobility shift *** sialoprotein (BSP),ALP,osteocalcin (OC),andosteopontin (OPN) gene transcription were also evaluated by either RT-PCR orNorthern ***:Genistein (0.01-1 μmol/L) dose dependently led tothe rapid and sustained activation of the p38 MAPK pathway in mouse *** with genistein (1 μmol/L) resulted in increased ALP activityand calcium deposition of BMSC cultures as a function of *** alsoenhanced Cbfa1 DNA binding activity and promoted the expressions of Cbfa1itself as well as several Cbfa1-regulated genes,including ALP,BSP,OC,and *** treatment with p38 MAPK inhibitor (SB203580) diminished thegenistein-induced osteoblastic maturation and p38 MAPK-Cbfa1 activation inmouse BMSC ***:These results indicated that genistein couldstimulate the osteoblastic differentiation of BMSC cultures through the p38MAPK-Cbfa1 pathway.

关键词： bone marrow-derived mesenchymal stem cells core-binding factor 1 osteoblastic differentiation genistein mitogen-activated protein kinase

Non-imaging-guided fine-needle aspiration of liver lesions:aretrospective study of 279 patients

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World Journal of Gastroenterology 1999年第2期5卷 10-14页

作者： Yeouda Edoute 1,4 , Ehud Malberger 2,4 , Orly Tibon Fisher 2 and Nimer Assy 3,4

AIM To determine the value of nonimaging-guided (direct) fine-needle aspiration cytology in diagnosing liver *** Detection by technetium-99m, ultrasound or computed tomographic scanning of the liver was made in 279 patients with 332 *** Based on histologic, cytologic and clinical findings, final liver diagnoses were reached in 265 patients, of whom 171 had malignant and 94 benign liver disease. Among the 171 patients with malignant liver disease, the cytologic findings indicated suspected malignancy in 8 patients, suggested definite malignancy in 130, but failed to disclose malignancy in 33 patients. In 93 of the 94 patients with benign liver disease, the cytologic findings were reported as benign, while in one patient the report of malignancy was false. The overall sensitivity, specificity, and positive and negative predictive values for cytologic results were 80.7%, 98.9%, 99.3% and 73.8%, respectively. The diagnostic accuracy of fine-needle aspiration cytology was 87.2%. The only major complication attributable to the procedure consisted of one case of *** Direct fine-needle aspiration of palpable liver mass and blind fine-needle aspiration of non-palpable liver lesions for cytodiagnosis are simple, safe, and cost-effective diagnostic method for evaluating the nature of liver lesions. The aspiration procedure including potential complications could be cut short by early finding of abnormal cells.

关键词： liver neoplasms/diagnosis liver neoplasms/secondary fine needle aspiration liver/pathology

Identification and classification of all potential hemolysin encoding genes and their products from Leptospira interrogans serogroup Icterohaemorrhagiae serovar Lai

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Acta Pharmacologica Sinica 2005年第4期26卷 453-461页

作者： Yi-xuan ZHANG~(2,3,4,5) Yan GENG(2,3,4,5) Bo BI~(2,4) Jian-yong HE~4 Chun-fu WU~4 Xiao-kui GUO~3 Guo-ping ZHAO~(2,4,6,7) 2 Research Center of Biotechnology,Shanghai Institutes for Biological Sciences,Chinese Academy of Sciences,Shanghai 200233,China 3 Department of Microbiology and Parasitology,Shanghai Second Medical University,Shanghai 200025,China 4 The School of Pharmaceutical Engineering,Shenyang Pharmaceutical University,Shenyang 110016,China 6 Chinese National Human Genome Center at Shanghai,ZhangJiang High Tech Park,Shanghai 201203,China Research Center of Biotechnology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai China Department of Microbiology and Parasitology Shanghai Second Medical University Shanghai China The School of Pharmaceutical Engineering Shenyang Pharmaceutical University Shenyang China Chinese National Human Genome Center at Shanghai ZhangJiang High Tech Park Shanghai China

Aim:To identify and classify all potential hemolysin candidates of Leptospirainterrogans serogroup Icterohaemorrhagiae serovar ***:All of thepotential hemolysin encoding genes were characterized in *** geneswere cloned and expressed in Escherichia *** hemolytic activities of theexpressed proteins were assayed observing the hemolysis on sheep blood *** activities of the hemolysin candidates were measuredby thin-layer chromatography(TLC)and HPLC for *** and secretion of the hemolysins in L interrogans were studied byreverse transcription polymerase chain reaction,Western blot,and enzyme-linkedimmunosorbent *** and Conclusion:The hemolytic activities ofhemolysin candidates(LA0327,LA0378,LA1027,LA1029,LAI650,LA3050,LA3937,LA4004)from L interrogans strain Lai were *** were furtherdivided into two groups,sphingomyelinase hemolysins and non-sphingomyelinasehemolysins,based on their ability to hydrolyze *** of thesehemolysins were actually expressed in living L interrogans and some of themwere secreted into the *** study establishes an essential findcomplete basis for further studying the contribution of hemolysins to the patho-genesis of L interrogans.

关键词： hemolysin Leptospira interrogans sphingo myelinase

ApoE ε4检测在阿尔茨海默病临床实践中的规范应用专家共识

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中国现代神经疾病杂志 2024年第8期24卷 657-667页

作者： ApoE ε4与阿尔茨海默病专家共识编写组中国人体健康科技促进会神经变性病专业委员会国家神经系统疾病临床医学研究中心烟台区域分中心巴茂文王刚不详青岛大学附属烟台毓璜顶医院神经内科、国家神经系统疾病临床医学研究中心烟台区域分中心、山东省神经免疫互作与调控重点实验室 264000 上海交通大学医学院附属仁济医院神经内科 200127

阿尔茨海默病是老年人群最常见的痴呆类型。载脂蛋白E ε4(ApoE ε4)基因是散发性阿尔茨海默病最主要的遗传风险因素,与其核心生物学标志物β-淀粉样蛋白(Aβ)和tau蛋白病理变化密切相关,是最具潜力的遗传生物学标志物。外周血ApoE ε4... 详细信息

阿尔茨海默病是老年人群最常见的痴呆类型。载脂蛋白E ε4(ApoE ε4)基因是散发性阿尔茨海默病最主要的遗传风险因素,与其核心生物学标志物β-淀粉样蛋白(Aβ)和tau蛋白病理变化密切相关,是最具潜力的遗传生物学标志物。外周血ApoE ε4基因检测在阿尔茨海默病风险预测和病情评估中起重要作用,但临床实践中存在对ApoE ε4基因检测认识不充分、重视程度不够等问题。特别是随着靶向Aβ的高质量临床药物试验的开展或临床药物治疗时代的到来,ApoE ε4基因检测的重要性日益突显。迄今国内尚缺乏ApoE ε4检测在阿尔茨海默病中规范应用的指南或共识。本文总结国内外ApoE ε4检测在阿尔茨海默病中的应用证据,并在此基础上撰写共识,旨在充分认识ApoE ε4检测在阿尔茨海默病中的临床价值并合理应用,提高疾病诊断与治疗水平,指引进一步的高质量临床研究。

关键词：阿尔茨海默病载脂蛋白E4 淀粉样β肽类生物标记诊疗指南

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A Series of Soliton-like and Double-like Periodic Solutions of a (2+1)-Dimensional Asymmetric Nizhnik-Novikov-Vesselov Equation

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Communications in Theoretical Physics 2004年第11期42卷 655-660页

作者： CHEN Yong,~(1,3,4, WANG Qi~(2,4) LI Biao~(2,4) ~1Department of Mathematics,Ningbo University,Ningbo 315211,China ~2Department of Applied Mathematics,Dalian University of Technology,Dalian 116024,China ~3Department of Physics,Shanghai Jiao Tong University,Shanghai 200030,China ~4Key Laboratory of Mathematics Mechanization~ the Chinese Academy of Sciences,Beijing 100080,China Department of Mathematics Ningbo University Ningbo 315211 China Department of Physics Shanghai Jiao Tong University Shanghai 200030 China Key Laboratory of Mathematics Mechanization the Chinese Academy of Sciences Beijing 100080 China Department of Applied Mathematics Dalian University of Technology Dalian 116024 China

We generalize the algebraic method presented by Fan [***.A:***.36 (2003) 7009)] to uniformlyconstruct a series of soliton-like solutions and double-llke periodic solutions for nonlinear partial differential equations(NPDE),As an application of the method,we choose a (2+1)-dimensional asymmetric Nizhnik-Novikov-Vesselov equa-tion and successfully construct new and more general solutions including a series of nontraveling wave and coefficientfunctions’ soliton-like solutions,double-like periodic and trigonometric-like function solutions.

关键词： symbolic computation soliton-like solution Weierstrass and Jacobi elliptic functions periodic solution

关于深化智慧城市发展推进城市全域数字化转型的指导意见

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安装 2024年第6期 1-3页

作者： 1.国家发展改革委 2.国家数据局 3.财政部 4.自然资源部国家发展改革委国家数据局财政部自然资源部

城市是推进数字中国建设的综合载体,推进城市数字化转型、智慧化发展,是面向未来构筑城市竞争新优势的关键之举,也是推动城市治理体系和治理能力现代化的必然要求。为深入贯彻落实党中央、国务院关于数字中国建设的决策部署,以数据融通... 详细信息

城市是推进数字中国建设的综合载体,推进城市数字化转型、智慧化发展,是面向未来构筑城市竞争新优势的关键之举,也是推动城市治理体系和治理能力现代化的必然要求。为深入贯彻落实党中央、国务院关于数字中国建设的决策部署,以数据融通、开发利用贯穿城市全域数字化转型建设始终,更好服务城市高质量发展、高效能治理、高品质生活,支撑发展新质生产力,推进中国式现代化城市建设,特制定本意见。一、总体要求以习近平新时代中国特色社会主义思想为指导,完整、准确、全面贯彻新发展理念,统筹发展和安全,充分发挥数据的基础资源和创新引擎作用,整体性重塑智慧城市技术架构、系统性变革城市管理流程、一体化推动产城深度融合,全面提升城市全域数字化转型的整体性、系统性、协同性,不断满足人民日益增长的美好生活需要,为全面建设社会主义现代化国家提供强大动力。

关键词：习近平新时代中国特色社会主义思想数字化转型全域高品质生活智慧化综合载体深度融合智慧城市

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Adiponectin as a negative regulator in obesity-related mammary carcinogenesis

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Cell Research 2007年第4期17卷 280-282页

作者： Yu Wang~(1,2,4) Karen SL Lam~(3,4) Aimin Xu~(3,4) ~1Genome Research Center,~2Department of Biochemistry,~3Department of Medicine,and 4Research Center of Heart,Brain,Hormone and Healthy Aging,University of Hong Kong,Hong Kong SAR,China Genome Research Center University of Hong Kong Hong Kong SAR China Department of Biochemistry University of Hong Kong Hong Kong SAR China Research Center of Heart Brain Hormone and Healthy Aging University of Hong Kong Hong Kong SAR China Department of Medicine University of Hong Kong Hong Kong SAR China

The prevalence of obesity and its associated diseases hasposed a huge healthcare impact on our *** is amajor risk factor for many serious medical conditions,suchas metabolic syndrome,Type 2 Diabetes and cardiovasculardisorders *** addition,the close association of obesitywith cancers has attracted significant attentions[1].Severalobesity-related cancers,including breast,prostate,endo-metrium,colon and gallbladder cancer,have a hormonalbasis and are life *** cancer is the mostfrequent cancer and the second leading cause of cancerdeath among *** adiposity over the pre-andpost-menopausal years is an independent risk factor for thedevelopment of breast cancer,and is also associated withlate-stage disease and poor prognosis[2].Adipose tissue has been shown to be an important playerin obesity-related mammary carcinogenesis[2].Adipocyteis one of the predominant stromal cell types in the microen-vironment of mammary *** is also the major site forlocal estrogen production from androgens by aromatase,

关键词： Res