Identification and classification of all potential hemolysin encoding genes and their products from Leptospira interrogans serogroup Icterohaemorrhagiae serovar Lai

作     者：Yi-xuan ZHANG~(2,3,4,5) Yan GENG(2,3,4,5) Bo BI~(2,4) Jian-yong HE~4 Chun-fu WU~4 Xiao-kui GUO~3 Guo-ping ZHAO~(2,4,6,7) 2 Research Center of Biotechnology,Shanghai Institutes for Biological Sciences,Chinese Academy of Sciences,Shanghai 200233,China 3 Department of Microbiology and Parasitology,Shanghai Second Medical University,Shanghai 200025,China 4 The School of Pharmaceutical Engineering,Shenyang Pharmaceutical University,Shenyang 110016,China 6 Chinese National Human Genome Center at Shanghai,ZhangJiang High Tech Park,Shanghai 201203,China 

作者机构：Research Center of Biotechnology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai China Department of Microbiology and Parasitology Shanghai Second Medical University Shanghai China The School of Pharmaceutical Engineering Shenyang Pharmaceutical University Shenyang China Chinese National Human Genome Center at Shanghai ZhangJiang High Tech Park Shanghai China 

出 版 物：《Acta Pharmacologica Sinica》 (中国药理学报(英文版))

年 卷 期：2005年第26卷第4期

页      面：453-461页

核心收录：

学科分类：1007[医学-药学(可授医学、理学学位)] 1002[医学-临床医学] 1001[医学-基础医学(可授医学、理学学位)] 10[医学] 

基　　金：Project supported by the National Natural Science Foundation of China (No 30370071) the National High Technoogy Research and Development Program of China(863 Program,No_ 2003,AA223030) 

主　　题：hemolysin Leptospira interrogans sphingo myelinase 

摘      要：Aim:To identify and classify all potential hemolysin candidates of Leptospirainterrogans serogroup Icterohaemorrhagiae serovar ***:All of thepotential hemolysin encoding genes were characterized in *** geneswere cloned and expressed in Escherichia *** hemolytic activities of theexpressed proteins were assayed observing the hemolysis on sheep blood *** activities of the hemolysin candidates were measuredby thin-layer chromatography(TLC)and HPLC for *** and secretion of the hemolysins in L interrogans were studied byreverse transcription polymerase chain reaction,Western blot,and enzyme-linkedimmunosorbent *** and Conclusion:The hemolytic activities ofhemolysin candidates(LA0327,LA0378,LA1027,LA1029,LAI650,LA3050,LA3937,LA4004)from L interrogans strain Lai were *** were furtherdivided into two groups,sphingomyelinase hemolysins and non-sphingomyelinasehemolysins,based on their ability to hydrolyze *** of thesehemolysins were actually expressed in living L interrogans and some of themwere secreted into the *** study establishes an essential findcomplete basis for further studying the contribution of hemolysins to the patho-genesis of L interrogans.