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Proteome analysis of peroxisomes from dark-treated senescent Arabidopsis leaves

Journal of Integrative Plant Biology 2018年第11期60卷 1028-1050页

作者： Ronghui Pan Sigrun Reumann Piotr Lisik stefanie tietz Laura J.Olsen Jianping Hu MSU-Department of Energy Plant Research Laboratory Michigan State UniversityEast LansingMI 48824USA Department of Molecular Cellularand Developmental BiologyUniversity of MichiganAnn ArborMIUSA Center of Organelle Research University of StavangerN-4021 StavangerNorway Department of Plant Biochemistry and Infection Biology Institute of Plant Science and MicrobiologyUniversity of HamburgD-22609 HamburgGermany Plant Biology Department Michigan State UniversityEast LansingMI 48824USA

Peroxisomes compartmentalize a dynamic suite of biochemical reactions and play a central role in plant metabolism, such as the degradation of hydrogen peroxide, metabolism of fatty acids, photorespiration, and the biosyn- thesis of plant hormones. Plant peroxisomes have been traditionally classified into three major subtypes, and in-depth mass spectrometry （MS）-based proteomics has been per- formed to explore the proteome of the two major subtypes present in green leaves and etiolated seedlings. Here, we carried out a comprehensive proteome analysis of perox- isomes from Arabidopsis leaves given a 48-h dark treatment. Our goal was to determine the proteome of the third major subtype of plant peroxisomes from senescent leaves, and further catalog the plant peroxisomal proteome. We identified a total of 111 peroxisomal proteins and verified the peroxisomal localization for six new proteins with potential roles in fatty acid metabolism and stress response by in vivo targeting analysis. Metabolic pathways compartmentalized in the three major subtypes of peroxisomes were also compared, which revealed a higher number of proteins involved in the detoxification of reactive oxygen species in peroxisomes from senescent leaves. Our study takes an important step towards mapping the full function of plant peroxisomes.

关键词： Proteome analysis of peroxisomes from dark-treated senescent Arabidopsis leaves Figure YFP ATF

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The early development of face processing——What makes faces special?

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Neuroscience Bulletin 2012年第6期28卷 765-788页

作者： stefanie Hoehl stefanie Peykarjou Department of Psychology University of HeidelbergHauptstr47-51 Heidelberg 69117Germany

In the present article we review behavioral and neurophysiological studies on face processing in adults and in early development. From the existing empirical and theoretical literature we derive three aspects that distinguish face processing from the processing of other visual object categories. Each of these aspects is discussed from a developmental perspective. First, faces are recognized and represented at the individual level rather than at the basic level. Second, humans typically acquire extensive expertise in individuating faces from early on in development. And third, more than other objects, faces are processed holistically. There is a quantitative difference in the amount of visual experience for faces and other object categories in that the amount of expertise typically acquired for faces is greater than that for other object categories. In addition, we discuss possible qualitative differences in experience for faces and objects. For instance, there is evidence for a sensitive period in infancy for building up a holistic face representation and for perceptual narrowing for faces of one＇s own species and race. We conclude our literature review with questions for future research, for instance, regarding the exact relationship between behavioral and neuronal markers of face processing across development.

关键词： face processing development infants visual expertise holistic processing event-related potential

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Simultaneous determination of five anti-epilepsy drugs in human plasma using liquid chromatography-mass spectrometry

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Science China Chemistry 2010年第11期53卷 2373-2378页

作者： stefanie WeiBig Institute of Pharmaceutical Analysis College of PharmacyWuhan University

A new liquid chromatography-mass spectrometry method for the determination of carbamazepine,clonazepam,alprazolam,estazolam and phenytoin in human plasma has been developed by using diazepam as an internal *** separation was performed on a Zorbax SB-C18 column(30 mm × 2.1 mm,3.5 ?m) with a mobile phase consisting of methanol and aqueous 25 mM ammonium acetate using gradient elution.A diethyl ether extraction method was used for the extraction of five anti-epilepsy *** final extract was injected for analysis by LC-MS/*** method was validated within the concentration range of 50-5000 ng mL-1 for five anti-epilepsy *** precision of the assay(RSD%) was less than 10% at all concentration levels within the tested *** method recoveries for all samples were more than 90%.The results indicate that the method is specific,sensitive and accurate,and suitable to study the pharmacokinetics,to adjust the dosage for individual administration,and to monitor the drug-concentration and drug abuse of the five anti-epilepsy drugs.

关键词： liquid chromatography-mass spectrometry anti-epilepsy drugs plasma concentration

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View on microRNAs as a potential tool to fight blindness: focus on Müller glia and gliosis

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Neural Regeneration Research 2022年第7期17卷 1501-1502页

作者： stefanie Gabriele Wohl The State University of New York College of OptometryDepartment of Biological and Vision SciencesNew YorkNYUSA

The neural retina is a part of the central nervous *** it lacks regenerative capacity,in an event of injury or disease,neuronal loss leads to visual impairment and often to ***,Müller glia(MG),the predominant glia in the retina,undergo a variety of molecular and cellular changes and discontinue to carry out their important regular functions in the tissue(e.g.,maintaining tissue homeostasis and nurturing neurons).

关键词： homeostasis predominant carry

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Review on Efficacy and Health Services Research Studies of Complementary and Alternative Medicine in Inflammatory Bowel Disease

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Chinese Journal of Integrative Medicine 2011年第6期17卷 403-409页

作者： stefanie Joos Department of General Practice and Health Services Research University Medical Hospital Heidelberg Voβstrasse 2 69120 HeidelbergGermany

Objective：To assess the evidence of the use and efficacy for complementary and alternative medicine（CAM） in inflammatory bowel disease（IBD）. Methods：A systematic literature search in MEDLINE was performed for randomized controlled trials（RCTs） in Crohn＇s disease and ulcerative ***,a selective literature search for health services research studies on the use of CAM in patients with IBD was ***：Health services research studies showed a high use of CAM in adult and pediatric patients with IBD *** contrast to the high use among IBD patients,there was a lack of high-quality data for many of the used CAM *** most of the studies showed positive results,the methodological quality of most studies was rather low;therefore,the results had to be interpreted with *** there were many studies for probiotics and fish oil,RCTs for the highly used method homeopathy, for most herbal products,and for traditional Chinese medicine methods apart from acupuncture RCTs were completely ***：The lack of high-quality studies might be the consequence of the problems associated with the funding of clinical trials involving ***,having the high user rates in mind,high-quality studies assessing efficacy and safety of those methods are urgently ***,there is a need for better representation of CAM in undergraduate and postgraduate medical education.

关键词： complementary and alternative medicine inflammatory bowel disease Crohn's disease ulcerative colitis health services research health services research

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Evolution of the Madrean-Tethyan disjunctions and the North and South American amphitropical disjunctions in plants

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Journal of Systematics and Evolution 2009年第5期47卷 331-348页

作者： stefanie M.ICKERT-BOND UA Museum of the North Herbarium and Department of Biology and Wildlife University of Alaska Fairbanks

The present paper reviews advances in the study of two major intercontinental disjunct biogeographic patterns:(i) between Eurasian and western North American deserts with the Mediterranean climate(the Madrean-Tethyan disjunctions);and(ii) between the temperate regions of North and South America(the amphitropical disjunctions).Both disjunct patterns have multiple times of *** amphitropical disjunctions have largely resulted from long-distance dispersal,primarily from the Miocene to the Holocene,with available data indicating that most lineages dispersed from North to South *** of recent studies on the Mediterranean disjuncts between the deserts of Eurasia and western North America support the multiple modes of origin and are mostly consistent with hypotheses of long-distance dispersal and the North Atlantic ***’s Madrean-Tethyan hypothesis,which implies vicariance between the two regions in the early Tertiary,has been favored by a few *** Beringian migration corridor for semiarid taxa is also supported in some cases.

关键词： amphitropical disjunctions biogeography Madrean-Tethyan disjunctions Mediterranean disjuncts North-South American disjunctions.

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Electrical Conductivity of Sintered Chromia Mixed with TiO_2,CuO and Mn-Oxides

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Journal of Materials Science & Technology 2006年第2期22卷 245-251页

作者： K.El-Menshawy H.P.Buchkremer F.tietz D.Stoever Metallurgy Dept. Atomic Energy Authority CairoEgypt Institute for Materials and Processes in Energy Systems IWV-1 Forschungszentrum Juelich GmbH JuelichGermany Institute for Materials and Processes in Energy Systems IWV-1 Forschungszentrum Juelich GmbH Juelich Germany

The electrical conductivity of sintered Cr2O3 mixed with 2% and 5% （in molar fraction） TiO2 or CuO was investigated in the temperature range 500-900℃ in air and in At/4 vol. pct H2 atmospheres. The effect of different Mn-oxides on the electrical conductivity of Cr2O3 was also studied under the same conditions. From the conductivity measurements it is established that additions of TiO2 change the defect structure of Cr2O3 and the effect of TiO2 on the electrical conductivity is controlled by TiO2 concentration as well as temperature and O2 partial pressure of the surrounding atmosphere. The conductivity of Cr2O3 increased in air and decreased in the At/H2 atmosphere by CuO additions. The effect of CuO was discussed with possible changes in the defect concentration in Cr2O3. Mixing of Cr2O3 with different Mn-oxides at the same Mn to metal atom fraction decreased the conductivity in air and in Ar/H2 atmospheres. No clear correlation between the spinel fraction and the changes in conductivity could be found.

关键词： Electrical conductivity Cr2O3 Mixing with TiO2 CuO Mn-oxides

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A Survey on 360°Images and Videos in Mixed Reality:Algorithms and Applications

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Journal of Computer Science & Technology 2023年第3期38卷 473-491页

作者：张方略赵军红张赟 stefanie Zollmann School of Engineering and Computer Science Victoria University of WellingtonWellington 6012New Zealand College of Media Engineering Communication University of ZhejiangHangzhou 310018China Department of Computer Science University of OtagoDunedin 9054New Zealand

Mixed reality technologies provide real-time and immersive experiences,which bring tremendous opportunities in entertainment,education,and enriched experiences that are not directly accessible owing to safety or *** research in this field has been in the spotlight in the last few years as the metaverse went *** recently emerging omnidirectional video streams,i.e.,360°videos,provide an affordable way to capture and present dynamic real-world *** the last decade,fueled by the rapid development of artificial intelligence and computational photography technologies,the research interests in mixed reality systems using 360°videos with richer and more realistic experiences are dramatically increased to unlock the true potential of the *** this survey,we cover recent research aimed at addressing the above issues in the 360°image and video processing technologies and applications for mixed *** survey summarizes the contributions of the recent research and describes potential future research directions about 360°media in the field of mixed reality.

关键词： 360°image mixed reality 360°image processing virtual reality scene reconstruction virtual reality content manipulation

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Hybrid Short-term Load Forecasting Method Based on Empirical Wavelet Transform and Bidirectional Long Short-term Memory Neural Networks

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Journal of Modern Power Systems and Clean Energy 2022年第5期10卷 1216-1228页

作者： Xiaoyu Zhang stefanie Kuenzel Nicolo Colombo Chris Watkins School of Artificial Intelligence Anhui UniversityHefei 230601China Department of Electronic Engineering Royal HollowayUniversity of LondonEgham HillEgham TW20 OEXUK Department of Computer Science Royal HollowayUniversity of LondonEgham HillEgham TW200EXUK

Accurate short-term load forecasting is essential to modern power systems and smart grids. The utility can better implement demand-side management and operate power system stably with a reliable load forecasting system. The load demand contains a variety of different load components, and different loads operate with different frequencies. The conventional load forecasting methods, e.g., linear regression(LR), auto-regressive integrated moving average(ARIMA), deep neural network, ignore the frequency domain and can only use time-domain load demand as inputs. To make full use of both time-domain and frequency-domain features of the load demand, a load forecasting method based on hybrid empirical wavelet transform(EWT) and deep neural network is proposed in this paper. The proposed method first filters noises via wavelet-based denoising technique, and then decomposes the original load demand into several sub-layers to show the frequency features while the time-domain information is preserved as well. Then, a bidirectional long short-term memory(LSTM) method is trained for each sub-layer independently. In order to better tune the hyperparameters, a Bayesian hyperparameter optimization(BHO) algorithm is adopted in this paper. Three case studies are designed to evaluate the performance of the proposed *** the results, it is found that the proposed method improves the prediction accuracy compared with other load forecasting method.

关键词： Load forecasting empirical wavelet transform(EWT) recurrent neural network data denoising Bayesian hyperparameter optimization(BHO)

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Intercontinental and intracontinental biogeography——patterns and methods

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Journal of Systematics and Evolution 2009年第5期47卷 327-329页

作者： stefanie M. ICKERT-BOND UA Museum of the North Herbarium and Department of Biology and Wildlife University of Alaska Fairbanks

The study of biogeography has benefited from the exponential increase of DNA sequence data from recent molecular systematic studies

关键词： DIVA patterns and methods

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