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Light(Science & Applications) 2013年第1期2卷 32-37页

作者： Zachary c Holman stefaan De Wolf christophe Ballif Swiss Federal Institute of Technology Lausanne(EPFL)Institute of Microengineering(IMT)Photovoltaics and Thin-Film Electronics LaboratoryCH-2000 NeuchaˆtelSwitzerland

Imperfect internal reflectance of near-bandgap light reduces the performance of all solar cells,and becomes increasingly detrimental as absorbers become *** consider light incident on the silicon/dielectric/metal structure at the back of rear-passivated crystalline silicon solar cells with surface textures that are large enough for geometric *** calculating the absorbance in the metal as a function of the angle of incidence,we discover three results that are important for understanding and improving rear reflectors in many types of solar ***,significant parasitic absorption occurs in the metal layer in two cases:s-and p-polarized propagating modes(near-normal angles of incidence)when the dielectric thickness is adjusted to cause destructive interference of the reflected beams,and p-polarized evanescent modes(angles of incidence above the semiconductor/dielectric critical angle)that excite surface plasmon polaritons at the metal ***,the latter loss dominates;a well-designed rear dielectric passivation layer must suppress the penetration of evanescent waves to the ***,when used as an input in a simple analytical model,the average rear internal reflectance calculated by assuming a Lambertian angular distribution of light accurately predicts the total reflectance and absorbance of a solar cell.

关键词： light trapping photovoltaics plasmon silicon solar cell

compact representations of microstructure images using triplet networks

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npj computational Materials 2020年第1期6卷 369-379页

作者： Michiel Larmuseau Michael Sluydts Koenraad Theuwissen Lode Duprez Tom Dhaene stefaan c.ttenier center for Molecular Modeling Ghent UniversityTechnologiepark 46B-9052 ZwijnaardeBelgium Department of Electrical Energy MetalsMechanical Constructions and SystemsGhent UniversityTechnologiepark 46B-9052 ZwijnaardeBelgium IDLab Department of Information TechnologyGhent University-IMECTechnologiepark 126B-9052 ZwijnaardeBelgium OcAS NV/ArcelorMittal Global R&D Gent Pres.J.F.Kennedylaan 3B-9060 ZelzateBelgium

The microstructure of a material,typically characterized through a set of microscopy images of two-dimensional cross-sections,is a valuable source of information about the material and its *** pixel of the image is a degree of freedom causing the dimensionality of the information space to be extremely *** makes it difficult to recognize and extract all relevant information from the *** experts circumvent this by manually creating a lower-dimensional representation of the ***,the question of how a microstructure image can be best represented remains *** the field of deep learning,we present triplet networks as a method to build highly compact representations of the microstructure,condensing the relevant information into a much smaller number of *** demonstrate that these representations can be created even with a limited amount of example images,and that they are able to distinguish between visually very similar *** discuss the interpretability and generalization of the *** compact microstructure representations,it becomes easier to establish processing–structure–property links that are key to rational materials design.

关键词： networks compact microstructure

Long-term live-cell microscopy with labeled nanobodies delivered by laser-induced photoporation

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Nano Research 2020年第2期13卷 485-495页

作者： Jing Liu Tim Hebbrecht Toon Brans Eef Parthoens Saskia Lippens chengnan Li Herlinde De Keersmaecker Winnok H.De Vos stefaan c.De Smedt Rabah Boukherroub Jan Gettemans Ranhua Xiong Kevin Braeckmans Laboratory of General Biochemistry and Physical Pharmacy Faculty of PharmacyGhent UniversityGhent B-9000Belgium Department of Biomolecular medicine Faculty of Medicine and Health SciencesGhent UniversityGhent B-9000Belgium VIB-UGent center for Inflammation Research VIBGhent B-9000Belgium VIB Bioimaging core Ghent VIBGhent B-9000Belgium Department of Biomedical Molecular Biology Ghent UniversityGhent B-9000Belgium Univ.Lille CNRSCentrale LilleISENUniv.ValenciennesUMR 8520-IEMNLille F-59000Franc Laboratory of cell Biology and Histology Department of Veterinary SciencesUniversity of Antwerp2020 AntwerpBelgium centre for Advanced Light Microscopy Ghent UniversityGhent B-9000Belgium

Fluorescence microscopy is the method of choice for studying intracellular ***,its success depends on *** of specific and stable markers.A prominent example of markers that are rapidly gaining interest are nanobodies(Nbs.-15 kDa),which can be functionalized with bright and photostable organic *** to their relatively small size and high specificity,Nbs offer great potential for high-quality long-term subcellular imaging,but suffer from the fact that they cannot spontaneously cross the plasma membrane of live *** have recently discovered that laser-induced photoporation is well suited to deliver extrinsic labels to living cells without compromising their *** a laser-based technology,it is readily compatible with light microscopy and the typical cell recipients used for *** by these promising initial results,we demonstrate here for the first time successful long-term imaging of specific subcellular structures with labeled nanobodies in living *** illustrate this using Nbs that target GFP/YFP-protein constructs accessible in the cytoplasm,actin-bundling protein Fascin,and the histone H2A/H2B *** an efficiency of more than 80%labeled cells and minimal toxicity(-2%),photoporation proved to be an excellent intracellular delivery method for ***-lapse microscopy revealed that cell division rate and migration remained unaffected,confirming excellent cell viability and *** conclude that laser-induced photoporation labeled Nbs can be easily delivered into living cells,laying the foundation for further development of a broad range of Nbs with intracellular targets as a toolbox for long-term live-cell microscopy.

关键词： laser-induced photoporation vapor nanobubble long-term microscopy imaging nanobody intracellular delivery living cell labeling

Intracellular Delivery of mRNA in Adherent and Suspension cells by Vapor Nanobubble Photoporation

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Nano-Micro Letters 2020年第12期12卷 404-420页

作者： Laurens Raes Stephan Stremersch Juan cFraire Toon Brans Glenn Goetgeluk Stijn De Munter Lien Van Hoecke Rein Verbeke Jelter Van Hoeck Ranhua Xiong Xavier Saelens Bart Vandekerckhove stefaan De Smedt Koen Raemdonck Kevin Braeckmans Laboratory of General Biochemistry&Physical Pharmacy Ghent University9000 GhentBelgium cancer Research Institute Ghent(cRIG) 9000 GhentBelgium Department of Diagnostic Sciences Ghent University9000 GhentBelgium VIB-UGent center for Medical Biotechnology 9052 GhentBelgium Department of Biomedical Molecular Biology Ghent University9000 GhentBelgium Department of Biochemistry and Microbiology Ghent University9000 GhentBelgium

Efficient and safe cell engineering by transfection of nucleic acids remains one of the long-standing hurdles for fundamental biomedical research and many new therapeutic applications,such as cAR T cell-based *** has recently gained increasing attention as a more safe and versatile alternative tool over viral-or DNA transposon-based approaches for the generation of adoptive T ***,limitations associated with existing nonviral mRNA delivery approaches hamper progress on genetic engineering of these hard-to-transfect immune *** this study,we demonstrate that gold nanoparticle-mediated vapor nanobubble(VNB)photoporation is a promising upcoming physical transfection method capable of delivering mRNA in both adherent and suspension *** transfection experiments on HeLa cells showed the importance of transfection buffer and cargo concentration,while the technology was furthermore shown to be effective for mRNA delivery in Jurkat T cells with transfection efficiencies up to 45%.Importantly,compared to electroporation,which is the reference technology for nonviral transfection of T cells,a fivefold increase in the number of transfected viable Jurkat T cells was ***,our results point toward the use of VNB photoporation as a more gentle and efficient technology for intracellular mRNA delivery in adherent and suspension cells,with promising potential for the future engineering of cells in therapeutic and fundamental research applications.

关键词： Transfection mRNA Photoporation Optoporation Gold nanoparticles Vapor nanobubbles

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聚合物纳/微纤维的制备及其应用

聚合物纳/微纤维的制备及其应用

Repeated photoporation with graphene quantum dots enables homogeneous labeling of live cells with extrinsic markers for fluorescence microscopy

2014年全国高分子材料科学与工程研讨会

作者：黄超伯 Jo Demeester stefaan c.De Smedt 南京林业大学化学工程学院药学院根特大学(Ghent University)

＜正＞由静电纺丝技术制备的纳米单纤维和无纺布用途非常广泛,本文介绍静电纺丝纤维在生物医药方面的三种应用。1.防伪材料:由静电纺丝技术、激光切割、编码等技术制备的数字编码纤维（见图1A）具有以下新颖性:a.取代传统防伪技术,直接... 详细信息

＜正＞由静电纺丝技术制备的纳米单纤维和无纺布用途非常广泛,本文介绍静电纺丝纤维在生物医药方面的三种应用。1.防伪材料:由静电纺丝技术、激光切割、编码等技术制备的数字编码纤维（见图1A）具有以下新颖性:a.取代传统防伪技术,直接对药片本身而不是包装盒进行防伪;b.该材料存储信息量大、生物相容和成本不高,非常适合在不富裕的发展中国家推广。2.智能型材料在艾滋病防治中的应用:经过近30年的研究,疫苗的研制尚未成功

关键词： Electrospinning Memofibers HIV inhibition cancer therapy

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Light(Science & Applications) 2018年第1期7卷 583-592页

作者： Jing Liu Ranhua Xiong Toon Brans Saskia Lippens Eef Parthoens Francesca cella Zanacchi Raffaella Magrassi Santosh K.Singh Sreekumar Kurungot Sabine Szunerits Hannelore Bové Marcel Ameloot Juan c.Fraire Eline Teirlinck Sangram Keshari Samal Riet De Rycke Gaëlle Houthaeve stefaan c.De Smedt Rabah Boukherroub Kevin Braeckmans Laboratory of General Biochemistry and Physical Pharmacy Faculty of PharmacyGhent UniversityGhent B-9000Belgium centre for Nano-and Biophotonics Ghent UniversityGhent B-9000Belgium VIB-UGent centre for Inflammation Research VIBGhent B-9000Belgium VIB Bioimaging core VIBGhent B-9000Belgium Department of Biomedical Molecular Biology Ghent UniversityGhent B-9000Belgium Nanophysics(NAPH) Istituto Italiano di TecnologiaGenova 16163Italy Biophysics Institute(IBF) National Research Council(CNR)Via De Marini6-16149–GE GenovaItaly Physical and Materials chemistry Division CSIR-National Chemical LaboratoryDr.Homi Bhabha RoadPune 411008India Academy of Scientific and Innovative Research Anusandhan Bhawan2 RafiMargNew Delhi 110001India Univ.Lille CNRSCentrale LilleISENUniv.ValenciennesUMR 8520-IEMNLille F-59000France Biomedical Research Institute Hasselt UniversityAgoralaan Building CDiepenbeek 3590Belgium centre for Surface chemistry and catalysis KU LeuvenCelestijnenlaan 200FLeuven 3001Belgium Inflammation Research center Image Core FacilityVIB9052 GhentBelgium Department of Biomedical Molecular Biology Ghent University9052 GhentBelgium Department of Plant Biotechnology and Bioinformatics Ghent University9052 GentBelgium Univ Lille 1 Univ Lille Nord FranceLab Phys Lasers Atomes&MolVilleneuve Dascq UMR 852359655France college of chemical Engineering Jiangsu Key Lab of Biomass-based Green Fuels and ChemicalsNanjing Forestry University(NFU)Nanjing 210037China UMR 8523 Laboratoire de Physique des LasersAtomes et MoléculesUniversitéde LilleVilleneuve d’AscqFrance IEMN UMR 8520Universitéde LilleVilleneuve d’AscqFrance

In the replacement of genetic probes,there is increasing interest in labeling living cells with high-quality extrinsic labels,which avoid over-expression artifacts and are available in a wide spectral *** calls for a broadly applicable technology that can deliver such labels unambiguously to the cytosol of living ***,we demonstrate that nanoparticle-sensitized photoporation can be used to this end as an emerging intracellular delivery *** replace the traditionally used gold nanoparticles with graphene nanoparticles as photothermal sensitizers to permeabilize the cell membrane upon laser *** demonstrate that the enhanced thermal stability of graphene quantum dots allows the formation of multiple vapor nanobubbles upon irradiation with short laser pulses,allowing the delivery of a variety of extrinsic cell labels efficiently and homogeneously into live *** demonstrate high-quality time-lapse imaging with confocal,total internal reflection fluorescence(TIRF),and Airyscan superresolution *** the entire procedure is readily compatible with fluorescence(super resolution)microscopy,photoporation with graphene quantum dots has the potential to become the long-awaited generic platform for controlled intracellular delivery of fluorescent labels for live-cell imaging.

关键词： quantum enable replace

Graft dilatation and Barrett’s esophagus in adults after gastric pullup and jejunal interposition for long-gap esophageal atresia

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世界胃肠内镜杂志:英文版(电子版) 2023年第9期15卷 553-563页

作者： Eleonora Sofie van Tuyll van Serooskerken Gabriele Gallo Bas L Weusten Jessie Westerhof Lodewijk AA Brosens Sander Zwaveling Jetske Ruiterkamp Jan BF Hulscher Hubertus GM Arets Arnold JN Bittermann David c van der Zee stefaan HAJ Tytgat Maud YA Lindeboom Department of Pediatric Surgery Wilhelmina Children’s HospitalUtrecht 3508 ABNetherlands Department of Pediatric Surgery University Medical Center GroningenGroningen 9713 GZNetherlands Department of Gastroenterology and Hepatology University Medical Center UtrechtUtrecht 3508 ABNetherlands Department of Gastroenterology and Hepatology University Medical Center GroningenGroningen 9713 GZNetherlands Department of Pathology University Medical Center UtrechtUtrecht 3508 ABNetherlands Department of Pediatric Surgery Amsterdam University Medical CenterAmsterdam 1105 AZNetherlands Department of Pediatric Pulmonology Wilhelmina Children’s HospitalUtrecht 3508 ABNetherlands Department of Pediatric Otorhinolaryngology Wilhelmina Children’s HospitalUtrecht 3508 ABNetherlands

BAcKGROUND Esophageal replacement(ER)with gastric pull-up(GPU)or jejunal interposition(JI)used to be the standard treatment for long-gap esophageal atresia(LGEA).changes of the ER grafts on a macro-and microscopic level however,are *** To evaluate long-term clinical symptoms and anatomical and mucosal changes in adolescents and adults after ER for *** A cohort study was conducted including all LGEA patients≥16 years who had undergone GPU or JI between 1985-2003 at two tertiary referral centers in the *** underwent clinical assessment,contrast study and endoscopy with *** was collected *** differences between JI and GPU patients,and associations between different outcome measures were assessed using the Fisher’s exact test for bivariate variables and the Mann-Whitney U-test for continuous *** with a P-valueconsidered statistically *** Nine GPU patients and eleven JI patients were *** age at follow-up was 21.5 years and 24.4 years,*** was reported in six GPU patients(67%)vs four JI patients(36%)(P=0.37).Dysphagia symptoms were reported in 64%of JI patients,compared to 22%of GPU patients(P=0.09).contrast studies showed dilatation of the jejunal graft in six patients(55%)and graft lengthening in four of these six *** revealed columnar-lined esophagus in three GPU patients(33%)and intestinal metaplasia was histologically confirmed in two patients(22%).No association was found between reflux symptoms and macroscopic anomalies or intestinal *** GPU patients(33%)experienced severe feeding problems vs none in the JI *** median body mass index of JI patients was 20.9 kg/m^(2) vs 19.5 kg/m^(2) in GPU patients(P=0.08).cONcLUSION The majority of GPU patients had reflux and intestinal metaplasia in 22%.The majority of JI patients had dysphagia and a dilated ***-up after ER for LGEA is essential.

关键词： Long-gap esophageal atresia Jejunal interposition Gastric pull-up Barrett’s esophagus Intestinal metaplasia Esophageal replacement

Differential diagnosis of gallstones by using hypericin as a fluorescent optical imaging agent

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World Journal of Gastroenterology 2016年第29期22卷 6690-6705页

作者： Marlein Miranda cona Ye-Wei Liu Antoine Hubert Ting Yin Yuan-Bo Feng Peter de Witte Etienne Waelkens Yan-Sheng Jiang Jian Zhang stefaan Mulier Qian Xia Gang Huang Raymond Oyen Yi-cheng Ni Department of Imaging and Pathology Faculty of Medicine Biomedical Sciences Group University HospitalsKU Leuven Department of cellular and Molecular Medicine Biomedical Sciences GroupKU Leuven Molecular Biodiscovery Laboratory Faculty of Pharmaceutical Sciences Biomedical Sciences GroupKU Leuven Laboratory of Translational Medicine Jiangsu Province Academy of Traditional Chinese Medicine Department of Nuclear Medicine Ren Ji Hospital School of Medicine Shanghai Jiao Tong University

AIM: To explore the feasibility of using hypericin as an optical imaging probe with affinity for cholesterol for differential fluorescent detection of human ***: cholesterol, mixed and pigment stones from cholecystectomy patients were incubated with hypericin or solvent. After 72 h, the stones were analysed for fluorescence(365 nm) and treated with 2-propanol/dimethyl sulfoxide for high performance liquid chromatography(HPLc) analysis. Rats with virtual gallbladder containing human cholesterol, mixed or pigment gallstones(VGHG) received 5 mg/kg hypericin or solvent and VGHG rats with cholesterol stones were given different hypericin doses(5-15 mg/kg). Twelve hours later, the stones were analysed at 365 nm. Biliary excretion and metabolites of hypericin were assessed in common bile duct(cBD) cannulated rats for 9 h using fluorospectrometry, HPLc and matrixassisted laser desorption/ionization-time-of-flight mass spectrometry(MALDI-TOF MS).RESULTS: Homogeneous high fluorescence was seen on cholesterol stones either pre-incubated with hypericin or extracted from VGHG rats receiving hypericin. Mixed stones showed a dotted fluorescent pattern, whereas pigment and solvent-treated ones lacked fluorescence. HPLc showed 7.68, 6.65 and 0.08 × 10^(-3) M of cholesterol in extracts from cholesterol, mixed, and pigment gallstones, respectively. Hypericin accounted for 2.0, 0.5 and 0.2 × 10-6 M in that order. On cholesterol stones from VGHG rats receiving different hypericin doses, a positive correlation was observed between dose and fluorescence. In the bile from cBD-cannulated rats, fluorescence represented 20% of the injected dose with two peaks in 9 h. HPLc analysis revealed that hypericin conjugates reached 60% of the peak area. By MALDI-TOF MS, hypericinglucuronide was detected. cONcLUSION: This study proves the potential use of hypericin for differential fluorescent detection of human gallstones regarding their chemical composition.

关键词： Differential detection Fluorescence Human gallstones Hypericin Rat model of cholelithiasis

同轴电纺聚氨酯/邻苯二甲酸醋酸纤维素pH值响应纤维应用于阴道内药物递送

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同轴电纺聚氨酯/邻苯二甲酸醋酸纤维素pH值响应纤维应用于阴道内...

Rat model of cholelithiasis with human gallstones implanted in cholestasis-induced virtual gallbladder

2016年全国高分子材料科学与工程研讨会

作者：华大威刘中车王芳高步红陈飞张启路熊燃华韩景全 Sangram Keshari Samal stefaan c.De Smedt 黄超伯化学工程学院江苏省生物质绿色能源与化学品重点实验室南京林业大学分析测试中心南京林业大学加拿大皇后大学(Queen's University)化学工程系高分子化学实验室赫尔辛基大学(University of Helsinki) 药学院根特大学(Ghent University) 材料科学与工程学院南京林业大学

＜正＞电纺纤维在诸多领域有广泛应用,其中包括生物医药,生物传感器,生物技术等[1]。本研究中我们采用同轴电纺技术制备了以聚氨酯为芯,以邻苯二甲酸醋酸纤维素为鞘的具有pH值响应的微纳纤维,并将其应用于阴道内药物递送。邻苯二甲酸醋...

＜正＞电纺纤维在诸多领域有广泛应用,其中包括生物医药,生物传感器,生物技术等[1]。本研究中我们采用同轴电纺技术制备了以聚氨酯为芯,以邻苯二甲酸醋酸纤维素为鞘的具有pH值响应的微纳纤维,并将其应用于阴道内药物递送。邻苯二甲酸醋酸纤维素（cAP）作为一种常用的药用辅料,其在pH值小于7时不溶解,而在pH大于7时溶解[2]。由于阴道内呈弱酸性,而人体精液pH为碱性,所以以cAP为鞘的同轴纤维可使其微纳纤维作为精液响应的药物递送

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World Journal of Methodology 2016年第2期6卷 154-162页

作者： Marlein Miranda cona Yewei Liu Ting Yin Yuanbo Feng Feng chen stefaan Mulier Yue Li Jian Zhang Raymond Oyen Yicheng Ni Department of Imaging and Pathology Faculty of Medicine Biomedical Sciences GroupKU Leuven Department of Surgery Leopold Park Clinic CHIREC Cancer Institute Laboratory of Translational Medicine Jiangsu Province Academy of Traditional Chinese Medicine

AIM:To facilitate translational research on cholelithiasis,we have developed a rat model of human gallstones by exploiting the unique biliopancreatic features of this ***:Under anesthesia,16 adult rats of equal genders underwent two times of abdominal ***,their common bile duct(cBD)was ligated to cause cholestasis by total biliary obstruction(TBO).On day 0,1,3,7,14,21 and 28 after TBO,magnetic resonance imaging(MRI)was conducted to monitor the dilatation of the cBD,and blood was sampled to analyze total serum bilirubin(TSB).Secondly,on day 30,the abdomen was re-opened and gallstone(s)collected from human patients were implanted in the dilated cBD asa virtual gallbladder(VGB),which was closed by suture *** rat cholelithiasis model was examined by MRI,clinical observation,microcholangiography and ***:All rats survived two *** ligation,the cBD was dilated to a stable size of 4 to30 mm in diameter on day 21-28,which became a *** rats initially showed signs of jaundice that diminished over time,which paralleled with the evolving TSB levels from 0.6±0.3 mg/d L before ligation,through a peak of 10.9±1.9 mg/dL on day 14,until a nearly normalized value after day *** dilated cBD with thickened wall allowed an incision for implantation of human gallstones of 1-10 mm in *** rat cholelithiasis was proven by in vivo MRI and postmortem microcholangiography and ***:A rat model cholelithiasis with human gallstones has been established,which proves feasible,safe,reliable,nontoxic and *** the gallstones of human origin,applications of this model may be of help in translational research such as optical detection and lysis of gallstones by systemic drug administration.

关键词： cholelithiasis Rat Gallbladder common bile duct cholestasis Bilirubin Gallstones