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In vitro screening of probiotic properties of Lactobacillus plantarum isolated from fermented milk product

Food Quality and Safety 2020年第4期4卷 213-223页

作者： soumitra nath Jibalok Sikidar Monisha Roy Bibhas Deb Department of Biotechnology Gurucharan CollegeSilcharIndia Institutional Biotech Hub Gurucharan CollegeSilcharIndia

Objectives:The screening of traditional fermented products is essential for the assessment of safety,security,and further development of functional foods for the well-being of human *** aim of the present study was to isolate and identify bacteria from fermented raw milk samples that exhibit health benefits upon ***:In order to confirm the isolates as probiotics,several in vitro assays were conducted to assess the probiotic properties of isolated *** initial screening includes tolerance to acid,bile,pancreatin,and *** cell surface properties demonstrate their interaction with mucosal epithelium,which includes hydrophobicity and auto-aggregation *** assessment was done by performing haemolytic test and antibiotic susceptibility *** antagonistic activity of probiotic strain was further evaluated against some pathogenic ***:Lactobacillus plantarum(***)isolated from fermented raw milk was preliminarily identified by biochemical tests and further confirmed using 16S rRNA *** isolate designated as *** strain GCC_19M1 demonstrated significant tolerance to low pH,0.3%bile,0.5%pancreatin,and 5%*** the presence of simulated gastric juice(at pH 3),the isolate exhibited a survival rate of 93.48-96.97%.Furthermore,the development of ecological niches in the human gut and their successful accumulation have been revealed by auto-aggregation and hydrophobicity *** of haemolytic activity ensures the non-virulent nature of the *** plantarum strain GCC_19M1 showed susceptibility towards gentamicin,tetracycline,kanamycin,meropenem,and ceftriaxone and exhibited an antagonistic effect on pathogenic ***:The obtained results conveyed that *** strain GCC_19M1 has strong probiotic potential,and its presence in the fermented raw milk products may serve as a potent functional probiotic food.

关键词： raw milk probiotic Lactobacillus plantarum gastric juice auto-aggregation hydrophobicity

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Fractional order prey-predator model incorporating immigration on prey:Complexity analysis and its control

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International Journal of Biomathematics 2024年第5期17卷 285-317页

作者： Md.Jasim Uddin Chandra nath Podder Departmentof Mathematics University of Dhaka Dhaka-1000Bangladesh

In this paper,the Caputo fractional derivative is assumed to be the prey-predator *** order to create Caputo fractional differential equations for the prey-predator model,a discretization process is first *** fixed points of the model are categorized *** identify requirements for the fixed points of the suggested prey-predator model's local asymptotic *** demonstrate analytically that,under specific parametric conditions,a fractional order prey-predator model supports both a Neimark-Sacker(NS)bifurcation and a Flip *** present evidence for NS and Flip bifurcations using central manifold and bifurcation *** parameter values and the initial conditions have been found to have a profound impact on the dynamical behavior of the fractional order prey-predator *** the bifurcation parameter is increased,the system displays chaotic *** simulations are shown to demonstrate chaotic behaviors like bifurcations,phase portraits,invariant closed cycles,and attractive chaotic sets in addition to validating analytical *** suggested prey-predator dynamical system's chaotic behavior will be controlled by the OGY and hybrid control methodology,which will also visualize the chaotic state for various biological parameters.

关键词： Prey-predator model Caputo fractional derivative Flip and Neimark-Sacker(NS)bifurcations immigration chaos control numerical simulation

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Patterns,Causes and Perceptions of Human-Large Carnivore Conflict in the Chitwan National Park,Nepal

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Journal of Resources and Ecology 2024年第4期15卷 838-849页

作者： Parwati TIWARI Bishnu Prasad BHATTARAI Jagan nath ADHIKARI Binod BHATTARAI Central Department of Zoology Institute of Science and TechnologyTribhuvan UniversityKathmandu 44600Nepal Department of Zoology Birendra Multiple CampusTribhuvan UniversityChitwan 44200Nepal Nepal Zoological Society Kathmandu 44600Nepal

One of the major management problems in and around protected areas is the intensifying conflict between local people and wildlife,especially large *** depredation and human fatalities caused by attacks of carnivores are found to be serious obstacles in conflict *** study aims to explore the patterns,costs,causes and perceptions of human large carnivore conflict in the Nawalpur area of Chitwan National *** patterns of livestock loss and human casualties due to large carnivores(Panthera tigris and Panthera pardus)were analysed using the secondary data reported to Chitwan National Park from 2001 to *** understand the people’s perception towards carnivores and wildlife conservation,150 victim respondents were asked,with one household selected from each *** 19-year study period,a total of 521 incidents caused by large carnivores were reported,which included 33 human casualties and 488 livestock *** was responsible for the maximum conflict incidents in *** total relief provided for human deaths and injuries was US$17524.41,whereas US$13702.18 was used to compensate for livestock depredation in the Nawalpur area by authorities of the Chitwan National *** than 64%of the respondents liked the presence of carnivores in their area and had a positive attitude toward the conservation of large carnivores,even though the carnivores were responsible for livestock depredation and human injury and ***’s satisfaction with the relief scheme provided by the government depended on ethnicity,gender,age class,occupation,education,insurance,and livestock ownership,but they believed the scheme was not ***,an effective relief scheme and awareness about the process of relief funding should be conducted in conflict *** awareness programs for local communities about large carnivores,their behavior,and preparing predator-proof corrals would be helpful in minimizing conflict in the study a

关键词： depredation human wildlife conflict leopard tiger relief fund

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Random Amplification Polymorphic DNA and Agro-Morphological Traits-Based Fingerprinting for Detection of Genetic Divergence in Indian Black Rice

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Rice science 2024年第4期31卷 366-370,I0004-I0014页

作者： Manisha SHARMA Dhananjay RATURI Vijay Rani RAJPAL Manju CHAUDHARY Soom nath RAINA Apekshita SINGH Amity Institute of Biotechnology Amity University Uttar PradeshSector-125UP 201313India Department of Botany Hansraj CollegeUniversity of DelhiDelhi 110007India

In this study, 32 black rice genotypes spanning diverse geographies in India were evaluated at both phenotypic and DNA sequence levels to gain insights into their genetic makeup. Significant variations were observed f... 详细信息

关键词： Indian Morphological Traits

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Dengue hemorrhagic fever with rectus sheath hematoma:A case report

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Asian Pacific Journal of Tropical Medicine 2024年第3期17卷 136-138页

作者： Boudhayan Bhattacharjee Hardik Patel Rucha Karad Vasireddy Teja Agnibho Mondal Soumendra nath Haldar Bibhuti Saha Department of Infectious Diseases and Advanced Microbiology School of Tropical MedicineKolkataIndia

Rationale:Acute pain is a medical emergency that requires prompt abdominal evaluation and ***,a mosquito-borne arboviral infection,can lead to complications such as acute abdominal *** concerns:A 72-year-old hypertensive female presented with high-grade intermittent fever with chills and rigors for four *** was diagnosed with dengue fever(NS1Ag-reactive)the day before admission.A contrast-enhanced computed tomography of the abdomen showed a hematoma along the rectus sheath which was managed conservatively as per surgical ***:Dengue hemorrhagic fever with rectus sheath ***:Blood transfusion and fluid ***:Ten days after discharge,the patient reproted no pain in the right iliac fossa and the size of the hematoma was significantly ***:Although it is rarely seen as a cause of acute abdomen,complaints of adnominal pain should never be ignored in critical or convalescent phase of dengue *** investigations should be promptly conducted as hematoma is often difficult to be diagnosed *** in diagnosis of rectus sheath hematoma can be fatal.

关键词： Dengue fever Rectus sheath hematoma Surgical emergency Acute appendicitis

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Optimizing Biodiesel Production from Karanja and Algae Oil with Nano Catalyst:RSMand ANN Approach

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Energy Engineering 2024年第9期121卷 2363-2388页

作者： Sujeet Kesharvani Sakhi Katre Suyasha Pandey Gaurav Dwivedi Tikendra nath Verma Prashant Baredar Energy Centre Maulana Azad National Institute of TechnologyBhopal462003India Department of Mechanical Engineering Maulana Azad National Institute of TechnologyBhopal462003India

This study delves into biodiesel synthesis from non-edible oils and algae oil sources using Response Surface Methodology(RSM)and an Artificial Neural Network(ANN)model to optimize biodiesel *** of *** and Karanja oils is utilized,aiming to reduce free fatty acid content to 1%through single-step *** reveals peak biodiesel yield conditions:1%catalyst quantity,91.47 min reaction time,56.86℃reaction temperature,and 8.46:1 methanol to oil molar *** ANN model outperforms RSM in yield prediction *** impact assessment yields an E-factor of 0.0251 at maximum yield,indicating responsible production with minimal *** analysis reveals significant cost savings:30%-50%reduction in raw material costs by using non-edible oils,10%-15%increase in production efficiency,20%reduction in catalyst costs,and 15%-20%savings in energy *** optimized process reduces waste disposal costs by 10%-15%,enhancing overall economic ***,the widespread adoption of biodiesel offers economic,environmental,and social benefits to a diverse range of stakeholders,including farmers,producers,consumers,governments,environmental organizations,and the transportation *** among these stakeholders is essential for realizing the full potential of biodiesel as a sustainable energy solution.

关键词： Non-edible oil algae RSM ANN optimization environmental factor

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Strengthening Active Aging through Older People’s Association and Economic Activity of the Older People in Nepal

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Advances in Aging Research 2024年第2期13卷 15-24页

作者： Hom nath Chalise James Brightman Central Department of Population Studies Tribhuvan University Kathmandu Nepal University of Central Florida Orlando Florida USA

Aging is a natural lifelong process ending in death. Many older people are living in poverty. Older people are generally considered dependent on others as they grow older. The purpose of this article is to explore the entrepreneurship activities of Nepalese older adults. Data for this study were collected from the project Help Age International (HAI) implemented in Nepal. Qualitative data observations and interviews were used to collect data. The findings of this study show the formation of the Older People’s Association (OPA) has supported many older people to participate outside the home in various social activities. Moreover, regular deposits through OPAs offer little help. OPAs support older people in their need of financial support to implement minor entrepreneurship. Older people who received support were pleased and were actively involved in their activities and also regularly deposited money in them. Subsequently, older people’s participation in social activities has increased and also helped to lower elderly abuse, loneliness, and depression. Local governments should promote such activities which will help with healthy aging.

关键词： Aging in Nepal Active Aging Older People’s Association Older People Healthy Aging

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Gas Chromatographic Method for Identification and Quantification of Commonly Used Residual Solvents in Pharmaceuticals Products

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American Journal of Analytical Chemistry 2024年第8期15卷 241-252页

作者： Sreekanta nath Dalal Pranab Kumar Das Department of Applied Chemistry & Chemical Engineering Faculty of Engineering & Technology University of Dhaka Dhaka Bangladesh Department of Applied Chemistry & Chemical Engineering Faculty of Engineering University of Rajshahi Rajshahi Bangladesh

Background: Impurities are not expected in the final pharmaceutical products. All impurities should be regulated in both drug substances and drug products in accordance with pharmacopeias and ICH guidelines. Three different types of impurities are generally available in the pharmaceutical’s product specification: organic impurities, inorganic impurities, and residual solvents. Residual solvents are organic volatile chemicals used or generated during the manufacturing of drug substances or drug products. Purpose: The aim of this study is to develop a cost-effective gas chromatographic method for the identification and quantification of some commonly used solvents—methanol, acetone, isopropyl alcohol (IPA), methylene chloride, ethyl acetate, tetrahydrofuran (THF), benzene, toluene, and pyridine—in pharmaceutical product manufacturing. This method will be able to identify and quantify the multiple solvents within a single gas chromatographic procedure. Method: A gas chromatography (GC) equipped with a headspace sampler and a flame ionization detector, and a column DB 624, 30-meter-long × 0.32-millimeter internal diameter, 1,8 μm-thick, Brand-Agilent was used to develop this method. The initial GC oven temperature was 40°C and held for 5 minutes. It was then increase to 80˚C at a rate of 2˚C per minute, followed by a further increase to 225˚C at a rate of 30˚C per minute, with a final hold at 225˚C for 10 minutes. Nitrogen was used as a carrier gas at a flow rate of 1.20 mL per minute. Dimethyl sulfoxide (DMSO) was selected as sample solvent. Results: The developed method is precise and specific. The percent RSD for the areas of six replicate injections of this gas chromatographic method was within 10.0 and the recovery result found within 80.0% to 120.0%.

关键词： Method Development Gas Chromatography Compendial Method GDP Specificity Recovery

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Performance Analysis of Support Vector Machine (SVM) on Challenging Datasets for Forest Fire Detection

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International Journal of Communications, Network and System Sciences 2024年第2期17卷 11-29页

作者： Ankan Kar Nirjhar nath Utpalraj Kemprai Aman Department of Computer Science Chennai Mathematical Institute Chennai India Department of Mathematics & Computer Science Chennai Mathematical Institute Chennai India Department of Data Science Chennai Mathematical Institute Chennai India

This article delves into the analysis of performance and utilization of Support Vector Machines (SVMs) for the critical task of forest fire detection using image datasets. With the increasing threat of forest fires to ecosystems and human settlements, the need for rapid and accurate detection systems is of utmost importance. SVMs, renowned for their strong classification capabilities, exhibit proficiency in recognizing patterns associated with fire within images. By training on labeled data, SVMs acquire the ability to identify distinctive attributes associated with fire, such as flames, smoke, or alterations in the visual characteristics of the forest area. The document thoroughly examines the use of SVMs, covering crucial elements like data preprocessing, feature extraction, and model training. It rigorously evaluates parameters such as accuracy, efficiency, and practical applicability. The knowledge gained from this study aids in the development of efficient forest fire detection systems, enabling prompt responses and improving disaster management. Moreover, the correlation between SVM accuracy and the difficulties presented by high-dimensional datasets is carefully investigated, demonstrated through a revealing case study. The relationship between accuracy scores and the different resolutions used for resizing the training datasets has also been discussed in this article. These comprehensive studies result in a definitive overview of the difficulties faced and the potential sectors requiring further improvement and focus.

关键词： Support Vector Machine Challenging Datasets Forest Fire Detection Classification

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Advances in DNA methylation and its role in cytoplasmic male sterility in higher plants

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Journal of Integrative Agriculture 2024年第1期23卷 1-19页

作者： Atiqur Rahman Hasan Sofiur Rahman Shakil Uddin Naima Sultana Shirin Akhter Ujjal Kumar nath Shamsun Nahar Begum Mazadul Islam Afroz Naznin Nurul Amin Sharif Ahmed Akbar Hossain Department of Genetics and Plant Breeding Bangladesh Agricultural UniversityMymensingh 2202Bangladesh Plant Breeding Division Bangladesh Institute of Nuclear AgricultureMymensingh 2202Bangladesh Tuber Crop Research Centre Bangladesh Agricultural Research Institute(BARI)Joydebpur 1701Bangladesh Horticulture Research Centre Bangladesh Agricultural Research Institute(BARI)Joydebpur 1701Bangladesh Biotechnology Division Bangladesh Agricultural Research Institute(BARI)Joydebpur 1701Bangladesh International Rice Research Institute Bangladesh OfficeDhaka 1213Bangladesh Division of Soil Science Bangladesh Wheat and Maize Research InstituteDinajpur 5200Bangladesh

The impact of epigenetic modifications like DNA methylation on plant phenotypes has expanded the possibilities for crop *** methylation plays a part in the regulation of both the chromatin structure and gene expression,and the enzyme involved,DNA methyltransferase,executes the methylation process within the plant *** regulating crucial biological pathways,epigenetic changes actively contribute to the creation of the ***,epigenome editing may assist in overcoming some of the drawbacks of genome editing,which can have minor off-target consequences and merely facilitate the loss of a gene’s *** drawbacks include gene knockout,which can have such off-target *** review provides examples of several molecular characteristics of DNA methylation,as well as some plant physiological processes that are impacted by these epigenetic changes in the *** also discuss how DNA alterations might be used to improve crops and meet the demands of sustainable and environmentally-friendly farming.

关键词： DNA methylation epigenetics CMS male sterility chromatin architecture gene expression higher plants

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