In vitro screening of probiotic properties of Lactobacillus plantarum isolated from fermented milk product

作     者：Soumitra Nath Jibalok Sikidar Monisha Roy Bibhas Deb Soumitra Nath;Jibalok Sikidar;Monisha Roy;Bibhas Deb

作者机构：Department of BiotechnologyGurucharan CollegeSilcharIndia Institutional Biotech HubGurucharan CollegeSilcharIndia 

出 版 物：《Food Quality and Safety》 (食品品质与安全研究（英文版）)

年 卷 期：2020年第4卷第4期

页      面：213-223页

核心收录：

学科分类：08[工学] 0836[工学-生物工程] 082203[工学-发酵工程] 0822[工学-轻工技术与工程] 

基　　金：Department of Biotechnology (DBT)  New Delhi 

主　　题：raw milk probiotic Lactobacillus plantarum gastric juice auto-aggregation hydrophobicity 

摘      要：Objectives:The screening of traditional fermented products is essential for the assessment of safety,security,and further development of functional foods for the well-being of human *** aim of the present study was to isolate and identify bacteria from fermented raw milk samples that exhibit health benefits upon ***:In order to confirm the isolates as probiotics,several in vitro assays were conducted to assess the probiotic properties of isolated *** initial screening includes tolerance to acid,bile,pancreatin,and *** cell surface properties demonstrate their interaction with mucosal epithelium,which includes hydrophobicity and auto-aggregation *** assessment was done by performing haemolytic test and antibiotic susceptibility *** antagonistic activity of probiotic strain was further evaluated against some pathogenic ***:Lactobacillus plantarum(***)isolated from fermented raw milk was preliminarily identified by biochemical tests and further confirmed using 16S rRNA *** isolate designated as *** strain GCC_19M1 demonstrated significant tolerance to low pH,0.3%bile,0.5%pancreatin,and 5%*** the presence of simulated gastric juice(at pH 3),the isolate exhibited a survival rate of 93.48-96.97%.Furthermore,the development of ecological niches in the human gut and their successful accumulation have been revealed by auto-aggregation and hydrophobicity *** of haemolytic activity ensures the non-virulent nature of the *** plantarum strain GCC_19M1 showed susceptibility towards gentamicin,tetracycline,kanamycin,meropenem,and ceftriaxone and exhibited an antagonistic effect on pathogenic ***:The obtained results conveyed that *** strain GCC_19M1 has strong probiotic potential,and its presence in the fermented raw milk products may serve as a potent functional probiotic food.