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The clinical impact of bacterial biofilms

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International Journal of Oral Science 2011年第2期3卷 55-65页

作者： Niels Hoiby Oana Ciofu Helle Krogh Johansen Zhi-jun Song Claus Moser Peter Ostrup Jenser soren molin Michael Givskov Tim Tolker-Nieisen Thomas Bjamsholt Department of Clinical Microbiology Rigshospitalet Institute for International Health Medical Microbiology and Immunology BloScience and Technology Biocentrum Danish Technical University

Bacteria survive in nature by forming biofilms on surfaces and probably most, if not all, bacteria （and fungi） are capable of forming biofilms. A biofilm is a structured consortium of bacteria embedded in a self-produced polymer matrix consisting of polysaccharide, protein and extracellular DNA. Bacterial biofilms are resistant to antibiotics, disinfectant chemicals and to phagocytosis and other components of the innate and adaptive inflammatory defense system of the body. It is known, for example, that persistence of staphylococcal infections related to foreign bodies is due to biofilm formation. Likewise, chronic Pseudomonas aeruginosa lung infections in cystic fibrosis patients are caused by biofilm growing mucoid strains. Gradients of nutrients and oxygen exist from the top to the bottom of biofilms and the bacterial cells located in nutrient poor areas have decreased metabolic activity and increased doubling times. These more or less dormant cells are therefore responsible for some of the tolerance to antibiotics. Biofilm growth is associated with an increased level of mutations. Bacteria in biofilms communicate by means of molecules, which activates certain genes responsible for production of virulence factors and, to some extent, biofilm structure. This phenomenon is called quorum sensing and depends upon the concentration of the quorum sensing molecules in a certain niche, which depends on the number of the bacteria. Biofilms can be prevented by antibiotic prophylaxis or early aggressive antibiotic therapy and they can be treated by chronic suppressive antibiotic therapy. Promising strategies may include the use of compounds which can dissolve the biofilm matrix and quorum sensing inhibitors, which increases biofilm susceptibility to antibiotics and phagocytosis.

关键词： bacterial biofilm biofilm infection antibiotic resistance quorum sensing

Tuning beneficial calcineurin phosphatase activation to counterα-synuclein toxicity in a yeast model of Parkinson’s disease

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Neural Regeneration Research 2025年第1期20卷 199-200页

作者： Srishti Chawla Mikael molin Thomas Nystrom Institute for Biomedicine Sahlgrenska AcademyCentre for Ageing and Health-AgeCapUniversity of GothenburgGothenburgSweden Department of Life Sciences Chalmers University of TechnologyGothenburgSweden

Calcineurin(CN)is a calcium-and calmodulindependent serine/threonine that has been studied in many model organisms including yeast,filamentous fungi,plants,and *** biological functions range from ion homeostasis and virulence in lower eukaryotes to T-cell activation in humans by human nuclear factors of activated *** is a heterodimeric protein consisting of a catalytic subunit,calcineurin A(Cna1p),which contains an active site with a dinuclear metal center,and a regulatory Ca^(2+) binding subunit called calcineurin B(Cnb1p)required to activate *** calcineurin B subunit has been highly conserved through evolution:For example,the mammalian calcineurin B shows 54%identity with calcineurin B from Saccharomyces cerevisiae.

关键词： activation dinuclear conserved

REMnO3（RE=Pr,Nd,Sm,Eu,Gd） perovskite as efficient catalysts for oxygen reduction reaction

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Journal of Rare Earths 2024年第11期42卷 2078-2087页

作者： Rakesh Ranjan Mahalik Siba soren Ipsha Hota A.K.Debnath K.P.Muthe Purnendu Parhi Department of Chemistry Ravenshaw University Technical Physics Division Bhabha Atomic Research Centre

Rare earth metal based perovskite emerges as important catalytic material for energy production and *** ABO3type materials are now the focus of research in the field of oxygen reduction and evolution ***,in this work we evaluated the oxygen reduction properties of the synthesized one-dimensional REMnO3（RE=Pr,Nd,Sm,Eu and Gd）.The one-dimensional REMnO3prepared by the hydrothermal synthesis route results in pure phase perovskite on *** perovskite was characte rized with X-ray diffraction,Fourier transform-infrared（FT-IR）,field emission-scanning electron microscopy（FE-SEM）,transmission electron microscopy（TEM） and X-ray photoelectron spectroscopy（XPS）.The oxygen reduction reaction activities were investigated with the help of electrochemical workstation that consists of three-electrode system immersed in 0.1 mol/L KOH electrolyte solution saturated with *** Koutechy-Levich plot obtained from rotating ring disk electrode shows all the prepared catalysts follow 4e-processes during oxygen *** effect of "A" site cation variation in REMnO3 on oxygen reduction reaction is ***3showed better onset potential for oxygen reduction reaction among the prepared rare earth ***3having high RE:Mn ratio（A-site rich co mpositions） promotes oxygen reduction reaction（ORR） *** the perovs kites showed good oxygen reduction property with high stability and methanol tolerance.

关键词： Electrocatalytic activity Oxygen reduction reaction (ORR) Perovskite Rare earths REMnO3 SmMnO3

Omics approaches in asthma research:Challenges and opportunities

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Chinese Medical Journal Pulmonary and Critical Care Medicine 2024年第1期2卷 1-9页

作者： molin Yue Shiyue Tao Kristina Gaietto Wei Chen Department of Biostatistics School of Public HealthUniversity of PittsburghPittsburghPA 15224USA Division of Pediatric Pulmonary Medicine UPMC Children’s Hospital of PittsburghUniversity of PittsburghPittsburghPA 15224USA

Asthma,a chronic respiratory disease with a global prevalence of approximately 300 million individuals,presents a significant societal and economic *** multifaceted syndrome exhibits diverse clinical phenotypes and pathogenic endotypes influenced by various *** advent of omics technologies has revolutionized asthma research by delving into the molecular foundation of the disease to unravel its underlying *** technologies are employed to systematically screen for potential biomarkers,encompassing genes,transcripts,methylation sites,proteins,and even the microbiome *** review provides an insightful overview of omics applications in asthma research,with a special emphasis on genetics,transcriptomics,epigenomics,and the *** explore the cutting-edge methods,discoveries,challenges,and potential future directions in the realm of asthma omics *** integrating multi-omics and non-omics data through advanced sta-tistical techniques,we aspire to advance precision medicine in asthma,guiding diagnosis,risk assessment,and personalized treatment strategies for this heterogeneous condition.

关键词： Asthma Genomics Transcriptomics Epigenomics Metagenomics Multi-omics integration

环状RNA circNEIL3高表达促进乳腺癌细胞增殖

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海军军医大学学报 2024年第6期45卷 725-731页

作者：陈航李墨林陈义重庆医科大学基础医学院细胞生物学与遗传学教研室重庆400016 四川省达州市万源市中心医院外二科达州636350 重庆医科大学生命科学研究院骨发育与再生实验室重庆400016

目的探究环状RNA circNEIL3在乳腺癌中的表达、生物学功能及其对细胞周期相关蛋白表达的影响。方法收集50例经病理证实的乳腺癌患者的临床病理资料及乳腺癌组织和癌旁正常组织样本。采用环状RNA测序分析3对乳腺癌组织与癌旁正常组织的... 详细信息

目的探究环状RNA circNEIL3在乳腺癌中的表达、生物学功能及其对细胞周期相关蛋白表达的影响。方法收集50例经病理证实的乳腺癌患者的临床病理资料及乳腺癌组织和癌旁正常组织样本。采用环状RNA测序分析3对乳腺癌组织与癌旁正常组织的差异表达环状RNA,利用RNA酶消化实验和核质分离实验验证差异表达环状RNA circNEIL3的特征。采用qPCR检测circNEIL3在乳腺癌组织和细胞中的表达,分析circNEIL3表达与患者临床病理资料的关系。采用CCK-8实验和集落形成实验检测circNEIL3对BT-549和MCF-7细胞增殖的影响,运用流式细胞术检测细胞凋亡及细胞周期变化,采用蛋白质印迹法检测细胞周期蛋白D1(CCND1)、细胞周期蛋白依赖性激酶(CDK)4、细胞周期蛋白E1(CCNE1)和CDK2蛋白的表达水平。结果乳腺癌组织与癌旁正常组织中差异表达的circNEIL3不易被RNA酶R降解,其主要分布于乳腺癌细胞的细胞质中。与癌旁正常乳腺组织及细胞相比,乳腺癌组织和细胞中circNEIL3的表达水平均增高(均P2 cm和高TNM分期(Ⅲ期)的乳腺癌患者多表现为circNEIL3高表达(均P<0.05)。circNEIL3过表达可增强乳腺癌细胞增殖活性,并上调CCND1、CDK4、CCNE1和CDK2的蛋白表达水平(均P<0.05)。敲低circNEIL3可诱导乳腺癌细胞凋亡(P<0.01),导致细胞周期阻滞于G1期(P<0.01)。结论乳腺癌组织和细胞中高表达的circNEIL3可通过调控细胞周期相关蛋白的表达促进乳腺癌细胞增殖。

关键词：乳腺肿瘤 circNEIL3 细胞增殖细胞凋亡细胞周期

冰雹形状及重复加载对叶表皮细胞壁仿生结构撞击损伤影响分析

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振动与冲击 2024年第3期43卷 227-232,240页

作者：王海文徐丹陈墨林叶仁传任鹏江苏科技大学船舶与海洋工程学院江苏镇江212000

为了提高纤维轻量化构件的冰雹载荷防护性能,基于高垂直刚度特性的叶表皮细胞壁仿生结构(a biomimetic isosceles trapezoid corrugated lattice cellular, bio-ITCLC),构建基体为涤纶纤维层合材料的仿生夹层结构数值模型,分析了冰雹形... 详细信息

为了提高纤维轻量化构件的冰雹载荷防护性能,基于高垂直刚度特性的叶表皮细胞壁仿生结构(a biomimetic isosceles trapezoid corrugated lattice cellular, bio-ITCLC),构建基体为涤纶纤维层合材料的仿生夹层结构数值模型,分析了冰雹形状、速度及重复撞击对叶表皮细胞壁仿生夹层结构力学行为及动态损伤的影响。结果表明:撞击接触力随冰雹形状的变化而改变,在相同撞击速度条件下,接触力峰值、初始上升率均随冰雹接触面的增大而提高,但加载时间减小;柱形及锥形冰雹的接触力峰值随撞击速度的增大而线性增长,半球形冰雹的接触力峰值与撞击速度呈抛物线关系。bio-ITCLC仿生夹层结构在柱形、半球形和锥形冰雹下的损伤模式分别为撞击区域屈曲压溃而引起纤维断裂、压痕导致的纤维断裂及基体开裂、仿生芯层与上层面板的分层失效。重复撞击并不改变仿生结构的失效模式,但bio-ITCLC仿生夹层结构的基体分层及累积损伤范围随冰雹重复撞击次数的增加而显著增大。

关键词：叶表皮细胞壁仿生结构(bio-ITCLC) 冰雹形状重复撞击损伤模式

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科学新闻 2024年第1期 60-61页

作者： soren Lipman 倪伟波加州大学伯克利分校

读博两个月后，我参加了一个讨论研究生院健康和残疾问题的焦点小组。我问一位教授能为参加资格考试的学生提供什么便利，因为口试是许多研究生项目的一个关键障碍。“我不知道我们是否提供了。”他回答道。我吓了一跳，作为一个患有创...

读博两个月后，我参加了一个讨论研究生院健康和残疾问题的焦点小组。我问一位教授能为参加资格考试的学生提供什么便利，因为口试是许多研究生项目的一个关键障碍。“我不知道我们是否提供了。”他回答道。我吓了一跳，作为一个患有创伤后应激障碍（PTSD）的人，我在考试时总能得到帮助。我很快意识到，作为一名残疾学生，如果想在研究生院取得成功，就要大声疾呼，为自己争取支持。

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黄培《含章馆唐诗选》考述

盐城师范学院学报（人文社会科学版） 2024年第1期44卷 103-108页

作者：由墨林山东大学儒学高等研究院山东济南250100

《含章馆唐诗选》是清康熙二年(1662)山东即墨诗人黄培所编的唐诗选本。康熙八年黄培因诗案被处以极刑,其生前著作多遭毁禁,存世罕见,这部存世的诗选具有相当高的文献和史料价值。《含章馆唐诗选》共选诗四百余首,以盛唐诗为主,尤其以... 详细信息

《含章馆唐诗选》是清康熙二年(1662)山东即墨诗人黄培所编的唐诗选本。康熙八年黄培因诗案被处以极刑,其生前著作多遭毁禁,存世罕见,这部存世的诗选具有相当高的文献和史料价值。《含章馆唐诗选》共选诗四百余首,以盛唐诗为主,尤其以杜甫的诗歌为多。其选诗情况反映了黄培深受明代高棅、李攀龙等人影响,继承复古思潮,坚持“诗必盛唐”的诗学主张。黄培尤其重视对安史之乱后杜甫诗歌的选录,寄托了政权变革之际知识分子的亡国之思。另外,诗选也为研究清初山左诗学发展提供了极好的研究资料。

关键词：黄培《含章馆唐诗选》清代文字狱诗案

大湾区纪录片的传播、交融与突破——以中国香港纪录片《无穷之路》为例

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粤海风 2024年第1期 23-29页

作者：刘茉琳广东技术师范大学文学与传媒学院

中国香港电视纪录片《无穷之路》在中国香港本土、内地网站以及官方与民间等不同圈层均收获好评,作为一部扶贫工程题材的纪录片,该作品以真诚的态度打动观众,同时在选材、拍摄、叙事等方面都呈现出独特质感。该片延续中国香港纪录片的... 详细信息

中国香港电视纪录片《无穷之路》在中国香港本土、内地网站以及官方与民间等不同圈层均收获好评,作为一部扶贫工程题材的纪录片,该作品以真诚的态度打动观众,同时在选材、拍摄、叙事等方面都呈现出独特质感。该片延续中国香港纪录片的民生传统,关注小人物与普通人命运,在不同视角的融合中实现了国家叙事的共情体验与文化建构,对于纪录片拍摄以及主流话语宣传均有很强借鉴作用。

关键词：《无穷之路》视角交融民生传统香港电视纪录片