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Je-Chun-Jun induced apoptosis of human cervical carcinoma HeLa cells

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Acta Pharmacologica Sinica 2004年第10期 132-139页

作者： Han-jung chae,Kyung-mi PARK,Geun-youn LEE,Gi-seup JEONG,Hyung-rae PARK,Hyung-min KIM,soo-wan chae,Shim-keun YOO,Hyung-ryong KIM Department of Pharmacology and the Institute of Cardiovascular Research School of Medicine Chonbuk National University Jeonju Chonbuk 464-701 South Korea Department of Gynecology School of Oriental Medicine Wonkwang University Iksan Chonbuk 470-739 South Korea Department of Dental Pharmacology and Nano-Science & Technology Research Institute School of Dentistry Wonkwang University Iksan Chonbuk 570-749 South Korea Department of Dental Pharmacology and Nano-Science & Technology Research Institute School of Dentistry Wonkwang University Iksan Chonbuk 570-749 South Korea Department of Dental Pharmacology and Nano-Science & Technology Research Institute School of Dentistry Wonkwang University Iksan Chonbuk 570-749 South Korea Department of Pharmacology College of Oriental Medicine Kyung Hee University Seoul South Korea Department of Pharmacology and the Institute of Cardiovascular Research School of Medicine Chonbuk National University Jeonju Chonbuk 464-701 South Korea Department of Gynecology School of Oriental Medicine Wonkwang University Iksan Chonbuk 470-739 South Korea Department of Dental Pharmacology and Nano-Science & Technology Research Institute School of Dentistry Wonkwang University Iksan Chonbuk 570-749 South Korea

AIM: To study the mechanism of Je-Chun-Jun (JCJ)-inducing the apoptosis of the human cervical carcinoma, HeLa cells. METHODS: The cell viability was assessed using MTT assay. The optical density was measured at 570 nm. The caspase activity was measured using 50 mmol/L of fluorogenic substrate, AC-DEVD-AMC (caspase-3), AC-VEID-AMC (caspase-8) or AC-LEHD-AFC (caspase-9). To confirm the expression of proteins, Western blotting was performed. To detect the characteristic of apoptosis chromatin condensation, HeLa cells were stained with Hoechst 33258 in the presence of JCJ. For the cell cycle analysis, HeLa cells were incubated with Propidium iodide (PI) solution. Fluorescence intensity of cell cycle was measured using flow cytometry system. RESULTS: The loss of viability occurred following the exposure of 10 g/L JCJ. Cells treated with 10 g/L JCJ for 3 d exhibited the apoptotic morphology (brightly blue-fluorescent condensed nuclei by Hoechst 33258-staining) and the reduction of cell volume. Cells incubated

关键词： herbal medicine apoptosis p38 MAPK HeLa cells

Inhibitory effects of coronary vasodilator papaverine on heterologously-expressed HERG currents in Xenopus oocytes

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Acta Pharmacologica Sinica 2007年第4期28卷 503-510页

作者： Cuk-seong KIM Nam LEE sook-jin SON Kyu-seung LEE Hyo-shin KIM Yong-geun KWAK soo-wan chae Sang-do LEE Byeong-hwa JEON Jin-bong PARK Department of Physiology College of MedicineChungnam National UniversityDaejeon 301131Korea Department of Pharmacology Chonbuk National UniversityMedical SchoolChonju 561-180Korea

Aim:To characterize the effects of papaverine on HERG channels expressed inXenopus oocytes as well as cardiac action potential in rabbit ventricular ***:Conventional microelectrodes were used to record action potential inrabbit ventricular *** currents were recorded by 2-electrode voltageclamp technique in Xenopus oocytes injected with HERG ***:Papa-verine increased the cardiac action potential duration in rabbit ventricular *** blocked heterologously-expressed HERG currents in a concentration-depen-dent manner (IC5071.03±4.75 μmol/L,NH 0.80,n=6),whereas another phosphodi-esterase inhibitor,theophylline （500 μmol/L）,did *** blockade of papaverineon HERG currents was not *** slope conductance measuredas a slope of the fully activated HERG current-voltage curves decreased from78.03±4.25 μS of the control to 56.84±5.33,36.06±6.53,and 27.09±5.50 μS （n=4） by30,100,and 300 μmol/L of papaverine,*** （100 μmol/L）caused a 9 mV hyperpolarizing shift in the voltage-dependence of steady-stateinactivation,but there were no changes in the voltage-dependence of HERG cur-rent *** blocked HERG channels in the closed,open,andinactivated ***:These results showed that papaverine blockedHERG channels in a voltage-and state-independent manner,which may mostlikely be the major mechanism of papaverine-induced cardiac arrhythmia reportedin humans.

关键词： papaverine HERG cardiac action potential

Therapeutic potential of Gastrodia elata Blume for the treatment of Alzheimer's disease

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Neural Regeneration Research 2013年第12期8卷 1061-1070页

作者： Guang-Biao Huang Tong Zhao Sushma Shrestha Muna Hong-Mei Jin Jong-Il Park Kyu-Sik Jo Bo-Hee Lee soo-wan chae Sun-Young Kim soo-Hyun Park Eun-Ock Park Eun-Kyung Choi Young-Chul Chung Department of Psychiatry Chonbuk National University Medical SchoolJeonju 561-756 Republic of Korea Muju Chunma Native Local Industrial Center Muju 568-844 Republic of Korea Clinical Trial Center for Functional Foods Chonbuk National University HospitalJeonju 561-756 Republic of Korea Department of Pharmacology Chonbuk National University Medical SchoolJeonju 561-756 Republic of Korea

Several studies have demonstrated that the Chinese herb Gastrodia elata Blume can protect against amyloid beta-peptide （Ap）-induced cell death. To investigate the possible therapeutic effects of Gastrodia elata Blume on Alzheimer＇s disease, we established a rat model of AIzheimer＇s disease by injecting A325-35 into bilateral hippocampi. These rats were intragastrically administered 500 or 1 000 mg/kg Gastrodia elata Blume per day for 52 consecutive days. Morris water maze tests showed that Gastrodia elata Blume treatment significantly improved the spatial memory of Alzheimer＇s disease rats. Congo red staining revealed that Gastrodia elata Blume significantly reduced the number of amyloid deposits in the hippocampus of these rats. Western blot analysis showed that choline acetyltransferase expression in the medial septum and hippocampus was significantly increased by the treatment of Gastrodia elata Blume, while EIIman method showed significant decrease in the activity of acetylcholinesterase in all three regions （prefrontal cortex, medial septum and hippocampus）. These findings suggest that long-term administration of Gastrodia elata Blume has therapeutic potential for Alzheimer＇s disease.

关键词： neural regeneration neuroprotective effect Traditional Chinese medicine Gastrodia elata Blume Alzheimer's disease Morris water maze test choline acetyltransferase acetylcholinesterase grants-supported paper neuroregeneration

Performance of the EDACS-ADP incorporating high-sensitivity troponin assay:Do components of major adverse cardiac events matter?

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World Journal of Emergency Medicine 2024年第3期15卷 175-180页

作者： Yedalm Yoo Shin Ahn Bora chae Won Young Kim Department of Emergency Medicine Asan Medical CenterUniversity of Ulsan College of MedicineSeoul 05505Republic of Korea

BACKGROUND:The accelerated diagnostic protocol(ADP)using the Emergency Department Assessment of Chest pain Score(EDACS-ADP),a tool to identify patients at low risk of a major adverse cardiac event(MACE)among patients presenting with chest pain to the emergency department,was developed using a contemporary troponin *** study was performed to validate and compare the performance of the EDACS-ADP incorporating high-sensitivity cardiac troponin I between patients who had a 30-day MACE with and without unstable angina(MACE I and II,respectively).METHODS:A single-center prospective observational study of adult patients presenting with chest pain suggestive of acute coronary syndrome was *** performance of EDACS-ADP in predicting MACE was assessed by calculating the sensitivity and negative predictive ***:Of the 1,304 patients prospectively enrolled,399(30.6%;95%confidence interval[95%CI]:27.7%–33.8%)were considered low-risk using the *** them,the rates of MACE I and II were 1.3%(5/399)and 1.0%(4/399),*** EDACS-ADP showed sensitivities and negative predictive values of 98.8%(95%CI:97.2%–99.6%)and 98.7%(95%CI:97.0%–99.5%)for MACE I and 98.7%(95%CI:96.8%–99.7%)and 99.0%(95%CI:97.4%–99.6%)for MACE II,***:EDACS-ADP could help identify patients as safe for early ***,when unstable angina was added to the outcome,the 30-day MACE rate among the designated lowrisk patients remained above the level acceptable for early discharge without further evaluation.

关键词： Chest pain Major adverse cardiac event Acute coronary syndrome Emergency department

Mechanisms Underlying the Antifatigue Effects of the Mycelium Extract of Cordyceps(Paecilomyces hepiali, CBG-CS-2) in Mice in the Forced Swimming Test

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Food and Nutrition Sciences 2015年第2期6卷 287-298页

作者： soo-wan chae Fusako Mitsunaga Su-Jin Jung Ki-Chan Ha Hong-Sig Sin Seung-Hwan Jang Shin Nakamura Department of Pharmacology Chonbuk National University Medical School Jeonju-si Republic of Korea Clinical Trial Center for Functional Foods (CTCF2) Chonbuk National University Hospital Jeonju-si Republic of Korea Intelligence and Technology Lab Inc. Kaizu Japan NPO Primate Agora Inuyama Japan Healthcare Claims & Management Inc. Jeonju-si Republic of Korea Chebigen Inc. Jeonju-si Republic of Korea

Cordyceps (CS) is used as an alternative medicine and functional food. We examined in vivo mechanisms underlying the antifatigue effects of the cultured mycelium extract of CS (CS extract) in forced swimming mice, a fatigue model that is induced by muscle exercise. Animals orally administered with CS extract significantly extended the loaded forced-swimming time, indicating its antifatigue effects. CS extract modulated the increased levels of blood IL-6 that was induced by forced swimming. CS extract protected the forced swimming-induced increase in NKp46 expression of splenic NK cells, suggesting regulation of fatigue-elicited hyper-reactivity by activated NK cells. By DNA microarray analysis of the quadriceps femoris muscle, it was uncovered that CS extract prevented the forced swimming-mediated upregulation of the expression of 5 genes associating with muscular inflammation (Ccl6, Ccl8, and Wfdc17) and muscle regeneration (Sfrp4 and Nfil3), whereas it regulated the downregulation in the expression of Svs5 participating in actin binding. CS extract exhibits the antifatigue effects through preventing IL-6 accumulation in blood, regulating NK cell activation in the spleen, and alleviating altered expression of genes related to inflammation, regeneration, and actin binding in the local muscle. Thus, CS extract is an effective functional food for preventing fatigue.

关键词： DNA Microarray IL-6 NK Cell Activation Muscle Inflammation

Nutrigenomic Study on Immunomodulatory Function of CordycepsMycelium Extract (Paecilomyces hepiali) in Mitomycin C–Treated Mice

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Food and Nutrition Sciences 2014年第22期5卷 2217-2224页

作者： soo-wan chae Fusako Mitsunaga Su-Jin Jung Ki-Chan Ha Hong-Sig Sin Seung-Hwan Jang Shin Nakamura Department of Pharmacology Chonbuk National University Medical School Jeonju Republic of Korea Clinical Trial Center for Functional Foods (CTCF2) Chonbuk National University Hospital Jeonju Republic of Korea Intelligence and Technology Lab Inc. Kaizu Japan NPO Primate Agora Inuyama Japan Healthcare Claims & Management Inc. Jeonju Republic of Korea Chebigen Inc. Jeonju Republic of Korea

Cordyceps (CS) is a Chinese herb that produces various effects including immune modulation, and now CS culture product is interested in the use as a functional food. We prepared CS mycelium culture extract (Paecilomyces hepiali, CBG-CS-2), CS extract, for functional foods. This study aimed to deduce the molecular mechanism of immunomodulatory effect of CS extract in Peyer’s patches (PPs), a main gut immune site, in mice that treated with mitomycin C (MMC), an immunosuppressing antibiotics. Nutrigenomics give us invaluable molecular information about both of foods and nutrition to improve or maintain good health. Here we performed nutrigenomics using DNA microarray to investigate the effect of CS extract on gene expression altered in PPs of the immunosuppressed mice. Interestingly, CS extract protected from the MMC-mediated downregulation of 22 genes, which are associated with IgA production and other immune response in PPs. These suggested that CS extract alleviated the downregulated expression of immune related genes in the gut immune site in an immunosuppressed state. Thus CS extract appears to be practical functional food for immunodepression and/or its related hypofunction.

关键词： DNA Microarray Functional Food Gene Expression Immunosuppression

Evaluation of Methane Yield on Mesophilic-Dry Anaerobic Digestion of Piggery Manure Mixed with Chaff for Agricultural Area

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Advances in Chemical Engineering and Science 2013年第4期3卷 227-235页

作者： Dong-Heui Kwak Mi-Sug Kim Jae-Seung Kim Young-Youl Oh soon-Ok Noh Byung-Ok So Su-Young Jung Su-Jin Jung soo-wan chae Department of Medical Nutrition Therapy Chonbuk National University Medical School Jeonju Republic of Korea

A mesophilic-dry anaerobic digestion process is valid in treating high-concentration substrates containing low moisture content. It has merits of lower wastewater discharge and lower heat capacity required in maintaining reactor temperature as compared with a thermophilic-wet anaerobic digestion process. In fact, chaff can be easily obtained in farming areas and used as a mixture substrate as one of bulking agents for controlling moisture and supplying carbon. For this reason, this study applies the chaff to improve livestock manure, which contains high moisture content and is discharged from domestic pig farms. This study aims at verifying its feasibility for improving methane production efficiency on a basis of BMP (Biochemical Methane Potential) assay obtained through a series of experiments. Finding results were methane gas production and gas production per volatile solid (VS) added, and methane gas production among biogas production were increased as the chaff added in the piggery manure was increased. According to experimental results for improving the methane production efficiency, mixture of the chaff and the piggery manure played an important role in controlling the moisture content and improving the methane gas production rate, and also verified its feasibility in the mesophilic-dry anaerobic digestion process indicating relatively less difficulty for operation and management.

关键词： Anaerobic Digestion Methane Chaff Biogas Piggery Manure

Unravelling the role of the combined effect of metallic charge transfer channel and SiO_(x) overlayer in the Zr/Si-Fe_(2)O_(3):Au:SiO_(x) nanorod arrays to boost photoelectrochemical water splitting

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Journal of Energy Chemistry 2024年第3期90卷 370-379,I0009页

作者： Tae Sik Koh Periyasamy Anushkkaran Love Kumar Dhandole Mahadeo A.Mahadik Weon-Sik chae Hyun Hwi Lee Sun Hee Choi Jum Suk Jang Division of Biotechnology SafetyEnvironment and Life Science InstituteCollege of Environmental and Bioresource SciencesJeonbuk National UniversityIksan 54896Republic of Korea Department of Integrative Environmental Biotechnology College of Environmental and Bioresource SciencesJeonbuk National UniversityIksan 54596Republic of Korea Extreme Materials Research Center Korea Institute of Science and Technology(KIST)Seoul 02792Republic of Korea Daegu Center Korea Basic Science InstituteDaegu 41566Republic of Korea Pohang Accelerator Laboratory Pohang University of Science and Technology(POSTECH)Pohang 37673Republic of Korea

Hematite(α-Fe_(2)O_(3)) based photoanodes have been extensively studied due to various intriguing features that make them viable candidates for a photoelectrochemical(PEC) water splitting ***,we propose a Zr-doped Fe_(2)O_(3) photoanode decorated with facilely spin-coated Au nanoparticles(NPs) and microwave-assisted attached Si co-doping in conjunction with a SiO_(x) overlayer that displayed a remarkable photocurrent density of 2.01 mA/cm^(2) at 1.23 V *** kinetic dynamics at the photoelectrode/-electrolyte interface was examined by employing systematic electrochemical *** Au NPs played a dual role in increasing PEC water ***,the Schottky interface that was formed between Au NPs and Zr-Fe_(2)O_(3) lectrode ensured the prevention of electron flow from the photoanode to the metal,increasing the number of available charges as well as suppressing surface charge ***,Au extracted photoholes from the bulk of the Zr-Fe_(2)O_(3) and transported them to the outer SiO_(x) overlayer,while the SiO_(x) overlayer efficiently collected the photoholes and promoted the hole injection into the ***,Si co-doping enhanced bulk conductivity by reducing bulk charge transfer resistance and improving charge carrier *** study outlines a technique to design a metallic charge transfer path with an overlayer for solar energy conversion.

关键词： Hematite Microwave attachment Au nanoparticles SiO_(x) overlayer Water splitting

Toward a comprehensive hypothesis of oxygen-evolution reaction in the presence of iron and gold

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Journal of Energy Chemistry 2024年第2期89卷 172-183,I0005页

作者： Negah Hashemi Jafar Hussain Shah Cejun Hu Subhajit Nandy Pavlo Aleshkevych Sumbal Farid Keun Hwa chae Wei Xie Taifeng Liu Junhu wang Mohammad Mahdi Najafpour Department of Chemistry Institute for Advanced Studies in Basic Sciences(IASBS)Zanjan 45137-66731Iran Center for Advanced Mössbauer Spectroscopy Mössbauer Effect Data CenterDalian Institute of Chemical PhysicsChinese Academy of SciencesDalian 116023LiaoningChina Key Lab of Advanced Energy Materials Chemistry(Ministry of Education) Renewable Energy Conversion and Storage CenterCollege of ChemistryNankai UniversityTianjin 300071China Advanced Analysis Center Korea Institute of Science and TechnologySeoul 02792Republic of Korea Institute of Physics Polish Academy of SciencesWarsaw 02-668Poland National and Local Joint Engineering Research Center for Applied Technology of Hybrid Nanomaterials Henan UniversityKaifeng 475004HenanChina Center of Climate Change and Global Warming Institute for Advanced Studies in Basic Sciences(IASBS)Zanjan 45137-66731Iran Research Center for Basic Sciences&Modern Technologies(RBST) Institute for Advanced Studies in Basic Sciences(IASBS)Zanjan 45137-66731Iran

This study investigates the effects of Fe on the oxygen-evolution reaction(OER)in the presence of *** distinct areas of OER were identified:the first associated with Fe sites at low overpotential(~330 mV),and the second with Au sites at high overpotential(~870 mV).Various factors such as surface Fe concentration,electrochemical method,scan rate,potential range,concentration,method of adding K_(2)Fe O_(4),nature of Fe,and temperature were varied to observe diverse behaviors during OER for Fe O_(x)H_(y)/*** amounts of Fe ions had a significant impact on OER,reaching a saturation point where the activity did not increase *** electronic interaction between Fe and Au ions was indicated by X-ray photoelectron spectroscopy(XPS)and electron paramagnetic resonance(EPR)*** situ visible spectroscopy confirmed the formation of Fe O_(4)^(2-)during *** situ Mossbauer and surfaceenhanced Raman spectroscopy(SERS)analyses suggest the involvement of Fe-based species as intermediates during the rate-determining step of OER.A lattice OER mechanism based on Fe O_(x)H_(y)was proposed for operation at low *** functional theory(DFT)calculations revealed that Fe oxide,Fe-oxide clusters,and Fe doping on the Au foil exhibited different activities and stabilities during *** study provides insights into the interplay between Fe and Au in OER,advancing the understanding of OER mechanisms and offering implications for the design of efficient electrocatalytic systems.

关键词： DFT calculation Fe/Au electrocatalysis Mechanism Oxygen evolution Water oxidation