Je-Chun-Jun induced apoptosis of human cervical carcinoma HeLa cells

作     者：Han-jung CHAE,Kyung-mi PARK,Geun-youn LEE,Gi-seup JEONG,Hyung-rae PARK,Hyung-min KIM,Soo-wan CHAE,Shim-keun YOO,Hyung-ryong KIM 

作者机构：Iksan Chonbuk 470-739 South Korea Department of Dental Pharmacology and Nano-Science & Technology Research Institute School of Dentistry Wonkwang University Iksan Chonbuk 570-749 South Korea Department of Dental Pharmacology and Nano-Science & Technology Research Institute Department of Pharmacology and the Institute of Cardiovascular Research School of Dentistry Wonkwang University Iksan Chonbuk 570-749 South Korea Department of Dental Pharmacology and Nano-Science & Technology Research Institute School of Dentistry Wonkwang University Iksan Chonbuk 570-749 School of Medicine South Korea Department of Pharmacology College of Oriental Medicine Kyung Hee University Seoul South Korea Department of Pharmacology and the Institute of Cardiovascular Research School of Medicine Chonbuk National University Jeonju Chonbuk National University Chonbuk 464-701 South Korea Department of Gynecology School of Oriental Medicine Wonkwang University Iksan Chonbuk 470-739 South Korea Department of Dental Pharmacology and Nano-Science & Technology Research Institute School of Dentistry Jeonju Wonkwang University Iksan Chonbuk 570-749 South Korea Chonbuk 464-701 South Korea Department of Gynecology School of Oriental Medicine Wonkwang University 

出 版 物：《Acta Pharmacologica Sinica》 (中国药理学报(英文版))

年 卷 期：2004年第10期

页      面：132-139页

核心收录：

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

基　　金：ThisworkwassupportedbyKoreaResearchFoundationGrant(KRF-EX0001)post-docfellowship(2001:Han-JungCHAE)ofKoreaScience&EngineeringFoundation. 

主　　题：herbal medicine apoptosis p38 MAPK HeLa cells 

摘      要：AIM: To study the mechanism of Je-Chun-Jun (JCJ)-inducing the apoptosis of the human cervical carcinoma, HeLa cells. METHODS: The cell viability was assessed using MTT assay. The optical density was measured at 570 nm. The caspase activity was measured using 50 mmol/L of fluorogenic substrate, AC-DEVD-AMC (caspase-3), AC-VEID-AMC (caspase-8) or AC-LEHD-AFC (caspase-9). To confirm the expression of proteins, Western blotting was performed. To detect the characteristic of apoptosis chromatin condensation, HeLa cells were stained with Hoechst 33258 in the presence of JCJ. For the cell cycle analysis, HeLa cells were incubated with Propidium iodide (PI) solution. Fluorescence intensity of cell cycle was measured using flow cytometry system. RESULTS: The loss of viability occurred following the exposure of 10 g/L JCJ. Cells treated with 10 g/L JCJ for 3 d exhibited the apoptotic morphology (brightly blue-fluorescent condensed nuclei by Hoechst 33258-staining) and the reduction of cell volume. Cells incubated