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Comparison of Protein Profiles in Sputum between COPD and Acute Exacerbation of COPD

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Open Journal of Respiratory Diseases 2012年第2期2卷 25-30页

作者： soo-taek uh Seung Ah Ko An soo Jang Sung Woo Park Yong-Hoon Kim Young-Ki Paik Choon Sik Park Division of Respiratory and Allergy Medicine Soon Chun Hyang University Hospital Seoul South Korea Division of Respiratory Medicine Soonchunhyang University Chunan Hospital Asan South Korea Genome Research Center for Allergy and Respiratory Disease Soonchunhyang University Bucheon Hospital Bucheon South Korea Yonsei Proteome Research Center Department of Biochemistry and Biomedical Science College of Life Science and Biotechnology Yonsei University Seoul South Korea

Background and objective: Chronic obstructive pulmonary disease (COPD) is characterized by progressive airflow limitation that is associated with an abnormal inflammatory response of the lung to noxious particles or gases. Cigarette smoking is the major risk factor for the development of COPD. This study evaluated the levels of cyclophilin B in sputa from patients with COPD and COPD with acute exacerbation (AECOPD). Materials and Methods: Two-dimensional electrophoresis was used for differential display proteomics. Western blotting was used to identify and quantify cyclophilin B in sputum from subjects with AECOPD and COPD. Results: Forty-nine protein spots differed in relative intensity between the AECOPD (n = 6) and COPD (n = 6) subjects. Twenty proteins showed increased expression in the sputum of AECOPD subjects, and 29 proteins were present at lower levels in AECOPD sputum compared with COPD sputum. One of these proteins was associated with cyclophilin B. Cyclophilin B concentrations were lower in sputum from subjects with COPD (n = 4) versus AECOPD (n = 4). Conclusion: The sputum proteomic analysis suggests that changes in various proteins are associated with the development of AECOPD.

关键词： Proteomics COPD Acute Exacerbation Chromatography Liquid Mass Spectrometry

Detection of Graphene Cracks By Electromagnetic Induction,Insensitive to Doping Level

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Computer Modeling in Engineering & Sciences 2019年第8期120卷 351-361页

作者： Taeshik Yoon Sumin Kang Tae Yeob Kang taek-soo Kim Department of Mechanical Engineering KAISTDaejeon 34141Korea Graphene Research Center KAISTDaejeon 34141Korea

Detection of cracks is a great concern in production and operation processes of graphene based devices to ensure uniform ***,we show a detection method for graphene cracks by electromagnetic *** time varying magnetic field leads to induced voltage signals on graphene,and the signals are detected by a *** measured level of induced voltage is correlated with the number of cracks in graphene *** correlation is attributed to the increasing inductive characteristic of defective graphene,and it is verified by electromagnetic simulation and radio frequency ***,we demonstrate that the induced voltage signal is insensitive to the doping level of *** work can potentially lead to the development of a high-throughput and reliable crack inspection technique for mass production of graphene applications.

关键词： Graphene,crack detection inductance electromagnetic induction insensitive to doping

Preface: Nano/Micro Structures in Application of Computational Mechanics

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Computer Modeling in Engineering & Sciences 2019年第8期120卷 235-237页

作者： Chang-Chun Lee Nien-Ti Tsou taek-soo Kim Department of Power Mechanical Engineering National Tsing Hua UniversityHsinchu 30013Taiwan Department of Material Science and Engineering National Chiao Tung UniversityHsinchu 30013Taiwan Department of Mechanical Engineering KAISTDaejeon 34141Korea

Many important issues of structures,such as lattice mismatch,compatibility,orientation,evolution,phase transition,surfaces,interfaces,defects,and domain engineering,have attracted considerable *** corresponding mechanisms of these issues have not been fully *** mechanics has been widely used to investigate such complexities in nano/microstructures in recent *** this special issue,leading experts in their respective fields are invited to present their new insight into the theoretical calculation and modeling of nano/*** articles are collected.A brief introduction on each article is provided below.

关键词： compatibility insight mechanisms

Effect of Solute Contents of Al-Ni-Sr Alloy on Glass Forming Ability and Thermal Stability

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Journal of Rare Earths 2004年第Z2期22卷 73-77页

作者： taek soo Kim J K Lee H J Kim J C Bae Advanced Materials R&D Center Korea Institute of Industrial Technology994-32 Dongchun-dongYeonsu-kuIncheon 406-130Korea Advanced Materials R&D CenterKorea Institute of Industrial Technology994-32 Dongchun-dongYeonsu-kuIncheon 406-130Korea Advanced Materials R&D CenterKorea Institute of Industrial Technology994-32 Dongchun-dongYeonsu-kuIncheon 406-130Korea Advanced Materials R&D CenterKorea Institute of Industrial Technology994-32 Dongchun-dongYeonsu-kuIncheon 406-130Korea

Glass forming ability (GFA) and structural characterization of a new amorphous Al-Ni-Sr alloy melt spun were investigated on the theory of the factors for the glass formation. The GFA was varied with the ratio of Ni to Sr in detail and successful amorphous phase dispersed with 2 ～ 5 nm precipitates was prepared from Al-Ni5-Sr3. Thermal stability and crystallization behavior of the amorphous phase were determined by continuous and isothermal heating technique on DCS, resulting that the crystallization occurs by the growth of fcc Al nanocrystals on the preexistent nuclei.

关键词： glass formation crystallization Al base amorphous alloys rapid solidification

Influence of different nitrogen application on flour properties,gluten properties by HPLC and end-use quality of Korean wheat

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Journal of Integrative Agriculture 2018年第5期17卷 982-993页

作者： Seong-Woo Cho Chon-Sik Kang taek-Gyu Kang Kwang-Min Cho Chul soo Park Department of Crop Science and Biotechnology Chonbuk National University National Institute of Crop Science Rural Development Administration Research Center of Bioactive Materials Chonbuk National University

This study was performed to identify how the different levels of nitrogen application affected the variances of gluten properties and end-use qualities and the differences of variances among Korean wheat cultivars. Protein and dry gluten content, SDS sedimentation volume and water absorption of Mixolab increased as nitrogen application increased. This ratio of the increase was higher in Korean wheat cultivars for bread than in Korean wheat cultivars for noodles and cookies. The proportion of（α＋β）-gliadin measured by reversed-phase high-performance liquid chromatography（RP-HPLC） increased, but the proportion of ω-and γ-gliadin decreased as the protein content increased. The Korean wheat cultivars for bread showed a high proportion of（α＋β）-gliadin increase, the Korean cultivars for noodles had a high proportion of γ-gliadin decrease and the Korean wheat cultivars for cookies had a high proportion of ω-gliadin decrease. However, there was no variation of the component in the proportion of glutenin component measured by RP-HPLC, even though the protein content was increased, but all of the protein fractions measured by size exclusion（SE）-HPLC were increased. The soluble monomeric protein showed a high proportion of Korean wheat cultivars for bread by the increase of protein content. Bread loaf volume increased by the increase of protein content but there were no variances in the ratio of increase among Korean wheat cultivars. The cookie diameter decreased with the increase of protein content, and this ratio of decrease was the highest in Korean wheat cultivars for cookies. The hardness of cooked noodles also increased by the increase of protein content but there were no variations in springiness and cohesiveness. The decrease proportion of ω-gliadin affected the increase of bread loaf volume, the hardness of cooked noodles, and the decrease of cookie diameter.

关键词： wheat gluten end-use quality cultivar nitrogen application

Isolation, identification, Pb(Ⅱ) biosorption isotherms and kinetics of a lead adsorbing Penicillium sp. MRF-1 from South Korean mine soil

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Journal of Environmental Sciences 2010年第7期22卷 1049-1056页

作者： Natarajan Velmurugan Grim Hwang Muthuswamy Sathishkumar Tae Kie Choi Kui-Jae Lee Byung-taek Oh Yang-soo Lee Department of Forest Science and Technology Institute of Agriculture and Life SciencesChonbuk National University College of Agriculture and Life Sciences Seoul National University Singapore-Delft Water Alliance National University of Singapore2 Engineering Drive 2 Division of Biotechnology College of Environment and Bioresources SciencesChonbuk National University

A heavy metal contaminated soil sample collected from a mine in Chonnam Province of South Korea was found to be a source of heavy metal adsorbing biosorbents. Chemical analyses showed high contents of lead （Pb） at 357 mg/kg and cyanide （CN） at 14.6 mg/kg in the soil. The experimental results showed that Penicillium sp. MRF-1 was the best lead resistant fungus among the four individual metal tolerant fungal species isolated from the soil. Molecular characterization of Penicillium sp. MRF-1 was determined using ITS regions sequences. Effects of pH, temperature and contact time on adsorption of Pb（Ⅱ） by Penicillium sp. MRF-1 were studied. Favorable conditions for maximum biosportion were found at pH 4 with 3 hr contact time. Biosorption of Pb（Ⅱ） gradually increased with increasing temperature. Efficient performance of the biosorbent was described using Langmuir and Freundlich isotherms. Adsorption kinetics was studied using pseudo first-order and pseudo second-order models. Biosorbent Penicillium sp. MRF-1 showed the maximum desorption in alkali conditions. Consistent adsorption/desorption potential of the biosorbent in repetitive cycles validated the efficacy of it in large scale. SEM studies given notes on surface modification of fungal biomass under metal stress and FT-IR results showed the presence of amino groups in the surface structure of the biosorbent. In conclusion, the new biosorbent Penicillium sp. MRF- 1 may potentially be used as an inexpensive, easily cultivatable material for the removal of lead from aqueous solution.

关键词： heavy metal resistant fungus Penicillium sp. biosorption isotherms kinetics

Effects of planting distance and depth on PTO load spectrum of a small riding-type transplanter

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International Journal of Agricultural and Biological Engineering 2020年第2期13卷 57-63页

作者： Wan-soo Kim Yeon-soo Kim taek-Jin Kim Kyu-Chul Nam Tae-Bum Kim Tae-Ho Han Ryu-Gap Im Yong-Hyeon Kim Yong-Joo Kim Department of Biosystems Machinery Engineering Chungnam National UniversityDaejeon 34134Republic of Korea Department of Smart Agriculture Systems Chungnam National UniversityDaejeon 34134Republic of Korea Convergence Agricultural Machinery Group Korea Institute of Industrial TechnologyGimje 54325Republic of Korea Certification WarrantyInspection and Standardization TeamKorea Agricultural Machinery Industry CooperativeCheonan 31041Republic of Korea Diesel Service Korea Co.Ltd. Daejeon 34357Republic of Korea Agricultural Machinery Certification Team Foundation of Agricultural Technology Commercialization and TransferIksan 54667Republic of Korea Gint Co.Ltd. Suwon 16225Republic of Korea

The aim of this study was to analyze the effects of the planting distance and depth on the power take-off(PTO)load spectrum of a small riding-type transplanter for the optimal design of the *** measure load data during actual planting operation,a load measurement system was developed using a torque sensor,a data acquisition system,and an *** experiments were conducted at four planting distances(26 cm,35 cm,43 cm,and 80 cm)and three planting depths(85 mm,105 mm,and 136 mm)in a field with similar soil *** measured load data were inverted into a load spectrum using rain-flow counting and Smith-Watson-Topper(SWT)*** safety factor of a transplanter according to the planting conditions was analyzed using the converted load spectrum and commercial *** load spectrum for all planting conditions showed torque ratios similar within a high cycle region of 108 to *** torque ratio increased when the planting depth increased and planting distance decreased in the low cycle region under less than 108 *** safety factors of the PTO driving gear and the driven gear increased as the planting distance increased at all planting *** the planting depth decreased at the same planting distance,the safety factor of the PTO gears *** results of this study might provide useful information for a transplanter PTO design considering the working load according to the various planting conditions.

关键词： transplanter planting distance planting depth power take-off load spectrum safety factor

Thermal Stabilization of EDFA gain by hybrid concatenation of antimony-doped silica EDF

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光学学报 2003年第S1期23卷 419-420页

作者： Young-Eun Im uh-Chan Ryu P. Babu Won-taek Han K. Oh David J. DiGiovanni Baishi Wang Anand Hariharan Department of Information and Communications K-JIST Kwangju 500712 Rep. of Korea OFS Laboratories 700 Moutain Ave. Murray Hill NJ 07974 USA OFS Fitel SPD 19 Schoolhouse Road Somerset NJ 08873 USA

Sb-doped silica EDF showed an opposite temperature dependent gain profile compared to Al-doped silica EDF. Concatenation of those two EDFs showed a gain variation less than ?0.4 dB over 40nm of C-band with the 15dB ga... 详细信息

关键词： range of

CAD/CAM for scalable nanomanufacturing:A network-based system for hybrid 3D printing

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Microsystems & Nanoengineering 2017年第1期3卷 135-145页

作者： Hae-Sung Yoon Hyun-taek Lee Ki-Hwan Jang Chung-soo Kim Hyunseo Park Dae-Wook Kim Kunwoo Lee Sangkee Min Sung-Hoon Ahn BK21 Plus Transformative Training Program for Creative Mechanical and Aerospace Engineers Seoul National UniversitySeoul 08826Republic of Korea Department of Mechanical and Aerospace Engineering Seoul National UniversitySeoul 08826Republic of Korea Department of Mechanical Engineering University of Wisconsin-MadisonMadisonWI 53706USA Institute of Advanced Machines and Design Seoul National UniversitySeoul 08826Republic of Korea

Micro-and nano-structuring have been highlighted over several decades in both science and engineering *** addition to continuous efforts in fabrication techniques,investigations in scalable nanomanufacturing have been pursued to achieve reduced feature size,fewer constraints in terms of materials and dimensional complexity,as well as improved process *** this study,based on recent micro-/nanoscale fabrication processes,characteristics and key requirements for computer-aided design and manufacturing(CAD/CAM)systems for scalable nanomanufacturing were *** include a process knowledge database,standardized processing,active communication,adaptive interpolation,a consistent coordinate system,and management of peripheral *** scalable nanomanufacturing,it is important to consider the flexibility and expandability of each process,because hybrid and bridging processes represent effective ways to expand process *** an example,we describe a novel CAD/CAM system for hybrid three-dimensional(3D)printing at the *** novel hybrid process was developed by bridging aerodynamically focused nanoparticle printing,focused ion beam milling,micromachining,and spincoating *** system developed can print a full 3D structure using various inorganic materials,with a minimum process scale of 50 *** most obvious difference versus CAD/CAM at‘conventional’scales is that our system was developed based on a network to promote communication between users and process *** the network-based system,it is also possible to narrow the gap among different processes/*** anticipate that this approach can contribute to the development of CAD/CAM for scalable nanomanufacturing and a wide range of hybrid processes.

关键词： Computer-aided design and manufacturing(CAD/CAM) nanomanufacturing network-based 3D printing