检索结果-南通市图书馆

Evaluation of parasitemia by qPCR in patients with chronic Chagas disease treated with benznidazole

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Asian Pacific Journal of Tropical Medicine 2023年第12期16卷 565-572页

作者： Tycha Bianca Sabaini Pavan Eros Antônio de Almeida Rodrigo Gonçalves de Lima Jamiro da silva Wanderley Luiz Cláudio Martins Sandra Cecília Botelho Costa Gláucia Elisete Barbosa marcon Departamento de Clínica Médica Faculdade de Ciências MédicasUniversidade Estadual de Campinas(Unicamp)CampinasSão PauloBrasil Fundação Oswaldo Cruz Fiocruz Mato Grosso do SulCampo GrandeMSBrasil

Objective:To evaluate parasitemia by qPCR in patients undergoing etiological treatment and followed in a Brazilian reference ***:Parasite load was quantified by qPCR in 32 participants with chronic Chagas disease who were treated with *** analyses were performed before and after the treatment and parasite loads were compared prior and 12/18 months post the ***:Thirty-two participants were recruited and treated with benznidazole,and 20 were *** events(AE)were observed in 22 out of 29 participants that had safety data(76%),and dermatological alterations were the most frequently observed *** the 20 participants analyzed,13 and 7 completed 12 and 18 months follow-up after the treatment,respectively.12 Months after the final treatment,Trypanosoma cruzi was detectable in 3 patients by qPCR;18 months after the final treatment,Trypanosoma cruzi was detectable per qPCR in 4 of the 7 ***,between 12 and 18 months,7 participants of the 20 initial follow-up cases showed positive qPCR,indicating treatment ***:qPCR can be used as an alternative method for evaluating the effectiveness of the etiological treatment of CD,and can be applied to analyze early therapeutic *** study showed that benznidazole therapy had limited effectiveness in treating chronic CD patients,thus emphasizing the importance of conducting continued research for developing more effective therapies and diagnosis for CD.

关键词： Trypanosoma cruzi Chagas disease Antitrypanosomal therapy Benznidazole qPCR Therapeutic failure

Educational interventions aimed at improving the oral health conditions of workers: A critical review

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Health 2012年第6期4卷 341-347页

作者： Luisa silva Lima sonia Cristina Lima Chaves Paloma Perez Castro Faculty of Denstrity Federal University of BahiaSalvadorBrazil

The aim of this research was to identify studies describing the educational and/or health-promoting interventions and strategies used in various programs of oral healthcare conducted in populations of industrial workers. A review of the Science, MEDLINE, LILACS and SCIELO databases was performed for the period between 1980 and 2009. The criteria for a publication to be in- cluded were: 1) the population had to consist of adult workers;and 2) the educational and/or health-promoting interventions had to have been described. A great variety of educational strategies was found, ranging from individual counseling to small-group discussions and instructions on oral hygiene with supervised tooth brushing. The presence of plaque and gingivitis were the most commonly used indicators of the effect of these interventions. Cost evaluation was also an important topic. In general, the indicators analyzed reflected positive results that included a reduction in the indicators themselves (plaque and gingivitis) and in the cost of the programs, suggesting a certain effect of the educational programs developed. Nevertheless, differences in forms of exposure according to the type of occupation are not discussed and neither are specific social determinants such as income and education level. The quality of the studies is limited, which reduces the robustness of the evidence on the effect of educational programs on the work population. Studies should be carried out using mixed approaches to evaluate the efficacy of these programs and propose improvements.

关键词： Occupational Health Oral Health Dental Health Education

Morphological and chemical aspects of Chlorella pyrenoidosa,Dunaliella tertiolecta,Isochrysis galbana and Tetraselmis gracilis microalgae

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Natural Science 2013年第7期5卷 783-791页

作者： Cristiane Mesquita da silva Gorgonio Donato Alexandre Gomes Aranda sonia Couri Department of Chemical Engineering Federal University of Rio de Janeiro(UFRJ)Rio de JaneiroBrazil Federal Education Institute Science and Technology of Rio de Janeiro(IFRJ)Rio de JaneiroBrasil

This study evaluates the growth and chemical composition of the following marine microalgae: Dunaliella tertiolecta, Isochrysis galbana, and Tetraselmis gracilis and the chemical composition of Chlorella pyrenoidosa. Microalgae can produce a number of compounds of high commercial value for the industry, mainly for the food industry. The growth kinetics, cell volume, pigments, carbohydrates, proteins, lipids, and fatty acid and amino acid composition were evaluated. I. galbana had the largest number of cells per mL-1 (107), concentration of carotenoids (6.33 μg·mL-1), and carbohydrates (34.32%). D. tertiolecta and T. gracilis had the highest cell volume (560.6 and 592.7 μm3, respectively), the highest amount of total dry biomass. D. tertiolecta had the highest chlorophyll concentration (9.05 μg·mL-1), and C. pyrenoidosa had the highest protein (48.16%) and lipid (14.30%) content. The marine species D. Tertiolecta, I. galbana, and T. gracilis had high levels of monounsaturated fatty acids (C18:1 n9), and C. pyrenoidosa was high in polyunsaturated fatty acids (C18:2 n6 and C18:3 n3), indicating the present high nutritional value fatty acids. The microalgae studies showed a composition of amino acids that meet the nutritional requirements recommended by the FAO g·100 g-1 (FAO/WHO/UN, 1985) for adults and children (2 - 5 years), indicating that these proteins can be used in foods.

关键词： Microalgae Macronutrients Chromatography

Role of Free Fatty Acids in Physiological Conditions and Mitochondrial Dysfunction

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Food and Nutrition Sciences 2013年第9期4卷 6-15页

作者： Zbigniew K. Binienda Sumit Sarkar sonia silva-Ramirez Carmen Gonzalez Autonomous University of San Luis Potosi San Luis Potosi Mexico. National Center for Toxicological Research/FDA Jefferson USA

The role of free fatty acids (FFAs) as a source of energy and their functions in energy transport within the body are well established. Equally important is a role that FFAs play in oxidative stress following cell membrane depolarization. FFAs are physiologically active, not only as nutritional components, but also as molecules involved in cell signaling and stabilization of membranes via palmitoylation and myristoylation. Protein palmitoylation is involved in numerous cellular processes, including apoptosis, and neuronal transmission. Besides nuclear peroxisome proliferator-activated receptors that mediate the biological effects of FFAs, G protein-coupled receptors (GPCRs) that are activated by FFA, have been recently identified. Those multiple FFA receptors (FFARs), which function on the cell surface as activated FFAs, play significant roles in the regulation of energy metabolism and mediate a wide range of important metabolic processes. FFARs have been targeted in drug development for the treatment of type 2 diabetes and metabolic syndrome. FFAs upregulate transcription of uncoupling proteins, increasing their expression in brain, cardiac, and skeletal muscle that may be protective or cytotoxic, depending on the cellular energy state. Recently, FFA effects on the endothelial function and dysfunction are being recognized. FFAs play a key role in endothelium-dependent nitric oxide production. A disturbance of endothelial function, due to an imbalance in production and release of relaxing and constricting factors, has implications in the development of cardiovascular problems, such as hypertension, as well as neurotoxicity following loss of blood-brain barrier integrity. This review presents information on broad range of FFAs actions of prime importance for physiological processes. Understanding of FFA functions in the body is crucial for developing new therapeutic strategies against several metabolic disorders.

关键词： Free Fatty Acids Beta-Oxidation Myristoylation Palmitoylation Endothelium

Association of Brazilian Adolescents with Healthy Eating: Knowledge, Perceptions and Food Choices

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Food and Nutrition Sciences 2011年第9期2卷 1036-1042页

作者： Ana Carolina Barco Leme sonia Tucunduva Philippi érika Christiane Toassa da silva 不详

The aim of this study is to identify the adolescents’ knowledge and perceptions of healthy and unhealthy eating. It is a qualitative cross section study with 83 adolescents of both genders between 10 and 16 years old enrolled in a public school in the city of S?o Paulo, Brazil. The participants answered two discursive questions that assessed their knowledge and perception of their actual diet. These questions were later analyzed by a qualitative technique, “Discourse of Collective Subject” based on the Theory of Social Representations. Overall, the youth presented an adequate knowledge about healthy eating. About half (52.6%) reported a balanced and varied diet;and 56.3% considered their actual diet as being healthy. Adolescents’ knowledge about healthy eating isn’t the only determinant for their food choices;and so traditional nutritional interventions focusing on concepts about healthy eating should be modified, aiming at behavioral changes.

关键词： Adolescents Healthy Eating Qualitative Study Knowledge and Perceptions

Exosomal glypican-1 is elevated in pancreatic cancer precursors and can signal genetic predisposition in the absence of endoscopic ultrasound abnormalities

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2022年第31期28卷 4310-4327页

作者： Pedro Moutinho-Ribeiro Ines A Batista Sofia T Quintas Bárbara Adem Marco silva Rui Morais Armando Peixoto Rosa Coelho Pedro Costa-Moreira Renato Medas Susana Lopes Filipe Vilas-Boas Manuela Baptista Diogo Dias-silva Ana L Esteves Filipa Martins Joanne Lopes Helena Barroca Fátima Carneiro Guilherme Macedo sonia A Melo Serviço de Gastrenterologia Centro Hospitalar Universitário de Sao JoaoPorto 4200Portugal Faculty of Medicine University of PortoPorto 4200Portugal Instituto de Investigacao e Inovacao em Saúde(i3S) Universidade do PortoPorto 4200Portugal IPATIMUP-Institute of Molecular Pathology and Immunology University of PortoPorto 4200Portugal Instituto de Ciencias Biomédicas Abel Salazar(ICBAS) University of PortoPorto 4050Portugal Servico de Cirurgia Geral Centro Hospitalar Universitário de Sao JoaoPorto 4200Portugal Unidade de Saúde Familiar Serpa Pinto ACeS Porto OcidentalPorto 4250Portugal Serviço de Anatomia Patológica Centro Hospitalar Universitário de Sao JoaoPorto 4200Portugal

BACKGROUND Individuals within specific risk groups for pancreatic ductal adenocarcinoma(PDAC)[mucinous cystic lesions(MCLs),hereditary risk(HR),and new-late onset diabetes mellitus(NLOD)]represent an opportunity for early cancer *** ultrasound(EUS)is a premium image modality for PDAC screening and precursor lesion *** no specific biomarker is currently clinically available for this purpose,glypican-1(GPC1)is overexpressed in the circulating exosomes(crExos)of patients with PDAC compared with healthy subjects or those harboring benign pancreatic *** To evaluate the capacity of GPC1+crExos to identify individuals at higher risk within these specific groups,all characterized by *** This cross-sectional study with a prospective unicentric cohort included 88 subjects:40 patients with MCL,20 individuals with HR,and 20 patients with NLOD.A control group(CG)was submitted to EUS for other reasons than pancreatic pathology,with normal pancreas and absence of hereditary risk factors(n=8).The inclusion period was between October 2016 and January 2019,and the study was approved by the Ethics Committee of Centro Hospitalar Universitário de São João,Porto,*** patients provided written informed *** and blood tests for quantification of GPC1+crExos by flow cytometry and carbohydrate antigen 19-9(CA 19-9)levels by ELISA were performed in all ***-guided tissue acquisition was done whenever *** statistical analysis,SPSS®27.0(IBM Corp.,Armonk,NY,United States)version was *** graphs were created using GraphPad Prism 7.00(GraphPad Software,San Diego,CA,United States).RESULTS Half of MCLs harbored worrisome features(WF)or high-risk stigmata(HRS).Pancreatic abnormalities were detected by EUS in 10.0%and 35.0%in HR and NLOD individuals,respectively,all considered non-malignant and“harmless.”Median levels of GPC1+crExos were statistically different:MCL[99.4%,interquartile range(IQR):94.9%-99.8%],HR(8

关键词： Glypican-1 Circulating exosomes Endoscopic ultrasound Pancreatic cancer risk groups Pancreatic cancer precursor lesions Genetic predisposition

Dietary supplementation with curcumin-loaded nanocapsules in lambs:Nanotechnology as a new tool for nutrition

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Animal Nutrition 2021年第2期7卷 521-529页

作者： Hiam marcon Luiz G.Griss Vitor L.Molosse Bruno G.O.Cecere Davi F.Alba Karoline W.Leal Gabriela M.Galli Carine F.Souza Matheus D.Baldissera Samanta Gundel Vitória de A.Bassotto Aline F.Ourique Marcelo Vedovatto Aleksandro S.Da silva Department of Animal Science Universidade Do Estado de Santa Catarina(UDESC)ChapecóSCBrazil Graduate Program in Animal Science UDESCChapecóSCBrazil Department of Physiology and Pharmacology Universidade Federal de Santa Maria(UFSM)RSBrazil Universidade Franscicana Santa MariaRSBrazil Graduate Program in Animal Science Universidade Estadual de Mato Grosso Do SulAquidauanaMSBrazil

Curcumin-containing nanocapsule powder formulations have not been used in ruminant feed to date,despite the fact that curcumin is known to be a functional food *** objective of this study was to determine whether ethyl polymethacrylate(Eudragit L-100)nanocapsules loaded with curcumin(N-CU)would improve health and growth of ***-two male Lacaune lambs(body weight[BW]=16±0.99 kg;45 d of age)were randomly assigned to 1 of 4 treatments:T0,T1,T2 and T4,representing supplementation of curcumin at 0,1,2,and 4 mg/kg concentrate,*** animals in each treatment were allocated in 4 pens of 2 lambs each(8 lambs per treatment).The experiment lasted 17 d,with samples and measurements collected on d 0,7,12,and *** T2 lambs had greater average daily gain than T0 *** analysis showed that the ideal dose of N-CU to enhance weight gain was 1.89 mg/kg *** were significant interactions(P<0.05)between treatments×time for hematological variables,particularly for increases in erythrocytes(T2)and re-ductions in counts of leukocytes,neutrophils,and lymphocytes in T1 and *** were significant interactions between treatment×time for total protein,globulin,urea,and triglyceride ***-lation of the antioxidant system was also *** were increased levels of non-protein thiols(NPSH),as well as increased activities of superoxide dismutase(SOD)and glutathione S-transferase(GST)in the supplemented *** of reactive oxygen species(ROS)were lower in the serum of sup-plemented *** general,the 4 mg/kg dose had no positive effects on growth or *** was an unexpected result,given the known properties of *** together,these findings suggest that addition of low concentrations of nanoencapsulated curcumin(T1 and T2)in lamb feed improves health,minimizing oxidative stress and generates anti-inflammatory effects that may have contributed indi-rectly to greater weight *** potentiate the effe

关键词： Antioxidant Anti-inflammatory Curcuma longa Performance Sheep

Whey Protein-Carboxymethylcellulose Obtained by Complex Coacervation as an Ingredient in Probiotic Fermented Milk

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Food and Nutrition Sciences 2015年第6期6卷 571-580页

作者： Maria Elisa Caetano-silva Caroline Dá rio Capitani Adriane Elisabete Costa Antunes Erna Vougt Vera sonia Nunes da silva Maria Teresa Bertoldo Pacheco Faculty of Food Engineering University of Campinas Campinas Brazil School of Applied Sciences University of Campinas Limeira Brazil Food Science and Technology Institute Federal University of Rio Grande do Sul Porto Alegre Brazil Chemistry Center of Food and Applied Nutrition Institute of Food Technology (ITAL) Campinas Brazil

Discharge of whey proteins is still a current practice by small cheese producers. The development of low-cost alternatives for recovery of these proteins is fundamental for small producers who cannot apply expensive techniques. The present study investigated the complex coacervation technique as a cheap technology to recover proteins from sweet whey using carboxymethylcellulose, and the coacervate used as an ingredient in the formulation of probiotic fermented milk. The nutritional properties of whey-carboxymethylcellulose coacervates (WP-CMC) were evaluated in trials with animals (rats) using casein as a reference. All these parameters—the coefficient of feed efficiency (CEA), protein digestibility-corrected amino acid score (PDCAAS), and net protein ratio (NPR), as well as weight gain—were determined to evaluate protein quality. A sensory acceptance test was applied to evaluate the sensory characteristics of the product. The complex coacervation technique recovered 86% of the protein from sweet whey. No significant (p > 0.05) differences were observed in the biological tests for both groups (WP-CMC and Casein groups) when NPR (4.98 to 5.04), digestibility (92.35 to 90.64), and CEA (0.40 to 0.42) were evaluated. Probiotic fermented milk beverage containing WP-CMC (0.78%) and guar gum (0.68%) presented good acceptability as determined by sensory evaluation. WP-CMC can be considered an ingredient with high nutritional and biological value that could be applied in probiotic fermented milk as an alternative to small producers to allocate the residual whey from cheese manufacture.

关键词： Nutritional Value Whey Proteins Complex Coacervation Probiotic Fermented Milk Environmental Impact

Morphological Structure Characterization of PAH/NiTsPc Multilayer Nanostructured Films

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Materials Sciences and Applications 2011年第11期2卷 1661-1666页

作者： Josmary R. silva Jackeline B. Brito sonia T. Tanimoto Nara C. de Souza 不详

Morphological structure and growth process of LbL nanostructured films from nickel tetrasulfonated phthalocyanine (NiTsPc) alternated with polyallylamine hydrochloride (PAH) were investigated. The experimental results of UV-visible adsorption kinetics (modeled by Johnson-Mehl-Avrami functions) and AFM images analyzes suggested that the surface morphology structure of films is formed by rod-shaped aggregates produced by a two-step growth process: nucleation and diffusion-limited growth.

关键词： Phthalocyanine Layer-By-Layer Films Growth Processes Adsorption Kinetics