检索结果-南通市图书馆

A prodrug nanoplatform via esterification of STING agonist and IDO inhibitor for synergistic cancer immunotherapy

Nano Research 2022年第10期15卷 9215-9222页

作者： Madiha Zahra Syeda Tu Hong Min Zhang Yanfei Han Xiaoling Zhu songmin ying Longguang Tang International Institutes of Medicine the Fourth Affiliated Hospital of Zhejiang University School of MedicineYiwu 322000China Department of Pharmacology and Department of Respiratory and Critical Care Medicine of the Second Affiliated Hospital Zhejiang University School of MedicineKey Laboratory of Respiratory Disease of Zhejiang ProvinceHangzhou 310009China

Cancer immunotherapy has made significant progress in the last few decades,revolutionizing ***,low patient response rates and potential immune-related adverse events continue to be major clinical *** nanomedicine,by virtue of its regulated delivery and modular flexibility,has shown the potential to strengthen antitumor immune responses and sensitize tumors to *** this study,we developed tumor microenvironment(TME)responsive nanomedicine to achieve specific and localized amplification of the immune response in tumor tissue in a safe and effective manner,while simultaneously reducing immune-related side *** synthesized the TME responsive prodrug by coupling MSA-2,a stimulator of interferon genes(STING)agonist,and NLG-919,an indoleamine 2,3 dioxygenase(IDO)*** prodrug was assembled into nanoparticles to enhance the solubility and *** synthesizing a TME responsive prodrug,we aim to explore the therapeutic efficacy of combined regimen(STING agonist and IDO inhibitor)for cancer,and reduce the unwanted side effects of STING agonism on normal *** prodrug and nanoparticles were characterized by mass spectrometry,dynamic light scattering(DLS),and transmission electron microscopy(TEM).Following that,we investigated the tumor accumulation,anti-tumor activity,and toxicity in vitro and in *** nanoparticles demonstrated the ability to inhibit the tumor growth and activate antitumor immune response by modulating immune cells populations in tumor *** TME responsive nanomedicine provided an effective tool for precise targeting,promoting antitumor immunity,and efficient tumor growth inhibition with *** of this study may have implications for future clinical trials.

关键词： prodrug esterase-responsive immunotherapy stimulator of interferon genes(STING) indoleamine 2 3 dioxygenase(IDO)

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

LPS-induced mitochondrial DNA synthesis and release facilitate RAD50-dependent acute lung injury

引用

Signal Transduction and Targeted Therapy 2021年第4期6卷 1104-1106页

作者： Xueqin Zhan Rui Cui Xinwei Geng Jiaqian Li Yunlian Zhou Lulu He Chao Cao Chao Zhang Zhimin Chen songmin ying Department of Pulmonology Children’s HospitalZhejiang University School of MedicineNational Clinical Research Center for Child HealthNational Children's Regional Medical CenterHangzhouZhejiangChina International Institutes of Medicine the Fourth Affiliated Hospital of Zhejiang University School of MedicineYiwuChina Department of Pharmacology and Department of Respiratory and Critical Care Medicine of the Second Affiliated Hospital Zhejiang University School of MedicineKey Laboratory of Respiratory Disease of Zhejiang ProvinceHangzhouZhejiangChina

Dear Editor,ATP-binding cassette(ABC)-ATPase(RAD50),together with meiotic recombination 11 homolog 1(MRE11)subunits,to form MRE11-RAD50 complex,plays important roles in recognition of double-stranded DNA(dsDNA)and initiation of consequent inflammatory *** lung injury(ALI)and acute respiratory destress syndrome(ARDS)are systemic uncontrolled inflammation and ***,the function of the DNA sensor in ALI/ARDS remains poorly *** we investigated functions of RAD50 using mouse primary macrophages and conditionally RAD50 knockout mice in vitro and in a lipopolysaccharide(LPS)-induced lung injury model.

关键词： RAD50 acute lung

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Eosinophil-derived chemokine(hCCL 15/23,mCCL6)interacts with CCR1 to promote eosinophilic airway inflammation

引用

Signal Transduction and Targeted Therapy 2021年第3期6卷 1046-1056页

作者： Xufei Du Fei Li Chao Zhang Na Li Huaqiong Huang Zhehua Shao Min Zhang Xueqin Zhan Yicheng He Zhenyu Ju Wen Li Zhihua Chen songmin ying Huahao Shen Key Laboratory of Respiratory Disease of Zhejiang Province Department of Respiratory and Critical Care Medicinethe Second Affiliated Hospital of Zhejiang University School of MedicineHangzhou 310009China Department of Anatomy Zhejiang University School of MedicineHangzhou 310058China Key Laboratory of Regenerative Medicine of Ministry of Education Institute of Aging and Regenerative MedicineJinan UniversityGuangzhouGuangdong 510632China International Institutes of Medicine Zhejiang University School of MedicineYiwu 322000China Department of Pharmacology Zhejiang University School of MedicineHangzhou 310058China State Key Lab of Respiratory Disease Guangzhou 510120China

Eosinophils are terminally differentiated cells derived from hematopoietic stem cells(HSCs)in the bone *** studies have confirmed the effective roles of eosinophils in asthmatic airway ***,their regulatory functions have not been well ***,increased C-C chemokine ligand 6(CCL6)in asthmatic mice and the human orthologs CCL15 and CCL23 that are highly expressed in asthma patients are described,which are mainly derived from *** Cc/6 knockout mice,further studies revealed CCL6-dependent allergic airway inflammation and committed eosinophilia in the bone marrow following ovalbumin(OVA)challenge and identified a CCL6-CCR1 regulatory axis in hematopoietic stem cells(HSCs).Eosinophil differentiation and airway inflammation were remarkably decreased by the specific CCR1 antagonist ***,the study identifies that the CCL6-CCR1 axis is involved in the crosstalk between eosinophils and HSCs during the development of allergic airway inflammation,which also reveals a potential therapeutic strategy for targeting G protein-coupled receptors(GPCRs)for future clinical treatment of asthma.

关键词： CCR1 inflammation airway

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Activating transcription factor 3 represses cigarette smoke-induced IL-6 and IL-8 expression via suppressing NF-κB activation

Activating transcription factor 3 represses cigarette smoke-...

引用

2016（第三十八届）浙江省呼吸系病学术年会

作者： Wu Yanping Cao Chao ying songmin Chen Zhihua Shen Huahao Li Wen Department of Respiratory and Critical Care Medicine Second Affiliated HospitalSchool of MedicineZhejiang University

OBJECTIVE:Airway and lung inflammation is a primary feature of chronic obstructive pulmonary disease(COPD).As a transcriptional modulator,activating transcription factor 3(ATF3)negatively regulates lots of proinflammatory cytokines and *** this study,we investigated the effects of ATF3 on the inflammatory response of *** AND METHODS:Cigarette smoke(CS)-induced model and cigarette smoke extract(CSE)-

关键词：

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Activating Transcription Factor 3 Is Essential for Cigarette Smoke-Induced Mucin Expression via Interaction with Activator Protein-1

Activating Transcription Factor 3 Is Essential for Cigarette...

引用

2016（第三十八届）浙江省呼吸系病学术年会

作者： Wu Yanping Wu Yinfang ying songmin Chen Zhihua Shen Huahao Li Wen Department of Respiratory and Critical Care Medicine Second Affiliated HospitalSchool of MedicineZhejiang University

OBJECTIVE:Our study investigated whether ATF3 is involved in the regulation of MUC5AC expression in COPD and explore the molecular *** AND METHODS:CS-induced ATF3 and MUC5AC expression levels were examined in HBE cells,human NCI-H292 cells and ***3 small interfering RNA(siRNA)and plasmid were used to investigate whether ATF3 is involved in CS-induced MUC5AC *** used quantitative real time PCR,Western Blot and immunofluorescence to assess whether the loss or gain of ATF3

关键词：

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

The Role of SETD8 in Genome Intergrity Maintenance and Tumoregenesis: Revelation from a Pair of Identical Twins

The Role of SETD8 in Genome Intergrity Maintenance and Tumor...

引用

2018年（第四十届）浙江省医学会呼吸系病学术年会

作者： Miao Li Hongwei Liao Chen Zhu Yanping Wu Yinfang Wu Xinwei Geng Zhihua Chen Huahao Shen songmin ying Wen Li Department of Respiratory and Critical Care Medicine Second Affiliated Hospital Institute of Respiratory Diseases Zhejiang University School of Medicine Department of Pharmacology Zhejiang University School of Medicine

Objective :Pulmonary sarcomatous neoplasms are a rare group of tumors occur in the lung[1]. We received a pair of monozygotic twins discordant for sarcomatoid neoplasms of the right principal bronchus in their early age. Identical twins were grown in the same environment and the genetic similarity was highly consistent, so we thought the very small number of genetic differences between the sisters may be involved in the occurrence of the disaster. As we all know, most human pathogenic genes are caused by mutations in exons. In order to find out novel genes related to the patient’s tumor, exome sequencing which has unique advantages in identifying the causative genes of rare diseases was *** : Exome sequencing was used in the twins mentioned above. Through comparison analysis among the twins and the general population, 11 genes were first screened out. After further siRNA screening of these genes, we identified H4K20-specific histone methyltransferase SETD8 as the most possible participant in tumor occurrence. Two individual siRNAs of SETD8 were used in U2OS and human bronchial epithelium(HBE) cells, cell proliferation, apoptosis, ageing, cell cycle were analyzed. To determine the effect of single nucleotide variation(SNV, T904C) in patient’s setd8, we overexpressed wildtype and mutant SETD8 respectively in cells, immunoprecipitation and mass spectrometry were followed to analysis the differences in interaction with other proteins, we also amplified wildtype and mutant SETD8 protein in vitro to assess their methyltransferase *** : : The silencing of SETD8 inhibited cell proliferation and increased apoptosis in both two cell lines. Cell cycle arrest and DNA damage also occurred without setd8. DNA fiber assay showed that compared with control, cells transfected with setd8 siRNA exhibited slower replication fork speed. Overexpress exogenous wildtype and mutant SETD8, protein interaction analysis revealed that the mutant SETD8 enhanced histone H4

关键词： Exome Sequencing SETD8 Genome Intergrity Methyltransferase TumourgenesisREFERENCE

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

SETD8C302R mutation revealed from myofibroblastoma-discordant monozygotic twins leads to p53/p21 deficit and WEE1 inhibitor sensitivity

SETD8C302R mutation revealed from myofibroblastoma-discordan...

引用

2020年（第四十二届）浙江省医学会呼吸系病学术大会

作者： Li Miao Wang Hongwu Liao Hongwei Shen Jiaxin Wu Yinfang Wu Yanping Weng Qingyu Zhu Chen Geng Xinwei Lan Fen Xia Yang Zhang Bin Zou Heng Zhang Nan Zhou Yunzhi Chen Zhihua Shen Huahao ying songmin Li Wen Department of Respiratory and Critical Care Medicine Emergency General Hospital

Objective Background High-throughput gene sequencing have identified a number of common genetic variants as the culprits for some cancers of high incidence, facilitating the development of targeted therapeutics. In contrast, rare deleterious mutations are much less understood because they are hidden in a myriad of nonsense genetic variations, which poses great challenge to the understanding of the pathology and thus the rational design of effective treatments for affected patients. Methods Germline DNA from a pair of monozygotic(MZ) twins discordant for lung inflammatory myofibroblastoma(IMT) were sequenced. With the healthy twin sister and the 1,000 genomes as the reference, candidate pathogenic mutations in the patient were screened out from the rare and potentially deleterious variants on the basis of the enrichment score. Promising candidates were further subjected to functional validation and therapeutic targeting evaluation. Results We identified a missense single nucleotide variation rs61955126 T>C in the lysine methyltransferase SETD8(Accession: NM20382, SETD8 C302 R) from the patient. In functional studies, we demonstrated that SETD8 was required for faithful DNA replication and genomic integrity, and its C302 R mutation downregulated the p53/21 pathway. Importantly, The SETD8 C302 R mutation conferred hypersensitivity of cancer cells to WEE1 inhibition. Conclusion Whole genome sequencing on monozygotic twins provides a unique opportunity for interrogating rare pathogenetic mutations. Using this strategy, we identified a rare mutation of SETD8(SETD8 C302 R), and unraveled its role in genome stability maintenance. Particularly, we revealed that cancer cells carrying SETD8 C302 R may be effectively treated by WEE1 inhibitor that is already under clinical trials.

关键词：

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

S100A8/RAGE、Caveolin-1在中性粒细胞性支气管哮喘大鼠中的作用及罗红霉素对其表达的影响

引用

中华结核和呼吸杂志 2019年第11期42卷 845-851页

作者：顾晓菲陈鑫淼陈慧君徐挺挺邱章伟孙丹丹葛翔挺应颂敏戴元荣温州医科大学附属第二医院育英儿童医院呼吸内科浙江325027 金华市中心医院呼吸内科浙江321000 杭州市余杭区第一人民医院感染内科 311100 浙江大学医学院附属第二医院呼吸与危重症医学科杭州310058

目的探讨S100A8、晚期糖基化终末产物受体(RAGE)、微囊蛋白1(Caveolin-1)在中性粒细胞性哮喘大鼠中的作用,以及罗红霉素干预其表达的可能机制。方法采用随机数字表法将18只Brown Norway雄性大鼠分为对照组、哮喘组、罗红霉素组,每组6只... 详细信息

目的探讨S100A8、晚期糖基化终末产物受体(RAGE)、微囊蛋白1(Caveolin-1)在中性粒细胞性哮喘大鼠中的作用,以及罗红霉素干预其表达的可能机制。方法采用随机数字表法将18只Brown Norway雄性大鼠分为对照组、哮喘组、罗红霉素组,每组6只。通过卵清白蛋白(OVA)和弗氏完全佐剂(FCA)腹腔注射联合致敏,OVA雾化激发构建大鼠哮喘模型,在每次激发前30 min,罗红霉素组大鼠予以罗红霉素30 mg/kg灌胃,对照组和哮喘组大鼠分别用等体积生理盐水代替。通过BALF中性粒细胞百分比(Neu%)及肺组织病理变化,确定哮喘模型的建立,检测各组大鼠BALF和血清中性粒细胞哮喘相关炎症因子表达,以及S100A8、RAGE和Caveolin-1蛋白含量的变化。结果哮喘组大鼠BALF中Neu%明显高于对照组(P<0.01);罗红霉素组BALF中Neu%明显低于哮喘组(P<0.01)。肺组织病理结果显示哮喘组大鼠支气管及血管周围、肺间质及肺泡腔内大量炎症细胞浸润,支气管壁及平滑肌明显增厚;罗红霉素组大鼠炎症表现及气道重塑情况较哮喘组减轻;对照组大鼠未见明显病理损害。哮喘组大鼠BALF和血清中炎症因子IL-6和IL-17水平明显高于对照组(均P<0.01),罗红霉素组IL-6和IL-17水平明显低于哮喘组(均P<0.05)。哮喘组大鼠BALF和血清中S100A8和RAGE水平明显高于对照组[(20.6±4.4)比(7.1±2.0)ng/L、(885±118)比(462±102)ng/L和(14.2±1.7)比(7.6±1.8)ng/L、(774±166)比(406±69)ng/L](均P<0.05);罗红霉素组S100A8和RAGE水平明显低于哮喘组[(14.3±3.7)比(20.6±4.4)ng/L、(650±53)比(885±118)ng/L和(10.4±1.2)比(14.2±1.7)ng/L、(537±81)比(774±166)ng/L](均P<0.05);同时哮喘组肺组织中Caveolin-1蛋白相对表达量明显低于对照组(P<0.01),罗红霉素组Caveolin-1蛋白相对表达量明显高于哮喘组(P<0.01)。相关性分析结果显示S100A8和RAGE表达呈正相关(r=0.706,P<0.01);S100A8和Caveolin-1表达呈负相关(r=-0.775,P<0.01);Caveolin-1和RAGE表达呈负相关(r=-0.919,P<0.01)。结论S100A8和Caveolin-1可能通过RAGE在中性粒细胞性哮喘中发挥重要作用,罗红霉素可能部分通过这条通路发挥抗炎及抑制气道平滑肌重塑作用。

关键词：中性白细胞哮喘气道重构炎症钙粒蛋白A 罗红霉素

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

“双思维”教学方式在生理科学实验课程中的应用探究

引用

中国高等医学教育 2024年第3期 65-66,69页

作者：程宁涛邵喆婳罗震宇应颂敏浙江大学医学院公共卫生学院浙江杭州310058 浙江大学医学院附属第二医院浙江杭州310009 浙江大学药学院浙江杭州310058 浙江大学“一带一路”国际医学院浙江义乌322000 浙江大学医学院附属第四医院浙江义乌322000

目的:本研究旨在探究“双思维”教学方式在本科生理科学实验教学中的应用效果,通过融合培养理论思维与实践思维的教学方式创新,为推动实验课程教学发展做出有益探索。方法:采用随机抽样方法,选取30名本科生作为试验组,以“双思维”教学... 详细信息

目的:本研究旨在探究“双思维”教学方式在本科生理科学实验教学中的应用效果,通过融合培养理论思维与实践思维的教学方式创新,为推动实验课程教学发展做出有益探索。方法:采用随机抽样方法,选取30名本科生作为试验组,以“双思维”教学方式进行授课。通过问卷调查,全面评估授课效果。结果:经过以“双思维”为核心的课程教学,学生的理论考试成绩并未显著提高;但从学生的主观反馈来看,该教学方式让他们对生命科学有了更深入的理解,提升了独立思考和实验操作能力,培养了良好的科研习惯,激发了科研热情。结论:“双思维”方式在生理科学实验课程教学中具有可行性和有效性。

关键词：双思维教学方式生理科学实验课程理论思维实践思维

来源：

维普期刊数据库

同方期刊数据库博看期刊评论

在线全文

学校读者我要写书评

暂无评论

RAGE在哮喘大鼠中的表达及罗红霉素的干预作用

引用

中华医学杂志 2019年第32期99卷 2542-2546页

作者：顾晓菲陈慧君陈鑫淼徐挺挺邱章伟吴立琴戴威应颂敏戴元荣温州医科大学附属第二医院、育英儿童医院呼吸内科温州325027 金华市中心医院呼吸内科金华321000 温州医科大学附属第二医院、育英儿童医院神经康复科温州325027 浙江大学医学院附属第二医院呼吸与危重症医学科杭州310058

目的探讨晚期糖基化终产物受体(RAGE)在哮喘大鼠中的表达及罗红霉素的干预作用。方法将18只雄性无特定病原体动物(SPF)级BrownNorway大鼠,按随机数字表法随机分为正常对照组、哮喘模型组、罗红霉素组各6只。通过卵白蛋白(OVA)+Al(OH)3... 详细信息

目的探讨晚期糖基化终产物受体(RAGE)在哮喘大鼠中的表达及罗红霉素的干预作用。方法将18只雄性无特定病原体动物(SPF)级BrownNorway大鼠,按随机数字表法随机分为正常对照组、哮喘模型组、罗红霉素组各6只。通过卵白蛋白(OVA)+Al(OH)3联合致敏,OVA雾化激发构建大鼠哮喘模型,在每次激发前30min,罗红霉素组予以罗红霉素30mg/kg灌胃,正常对照组和哮喘模型组分别用等体积生理盐水代替。采用酶联免疫吸附测定(ELISA)法检测各组大鼠血清和支气管肺泡灌洗液(BALF)中RAGE、白细胞介素(IL)-4的浓度;HE染色法观察各组大鼠肺组织气道炎症和细胞浸润情况;Image-ProPlus图像分析软件测量并计算支气管壁厚度及平滑肌厚度;Western印迹法检测各组大鼠肺组织RAGE蛋白相对表达量。结果哮喘模型组血清和BALF中RAGE、IL-4浓度显著均高于正常对照组[(494±32)比(327±45)ng/L、(32.4±5.8)比(13.1±2.9)ng/L和(553±38)比(399±56)ng/L、(37.8±3.4)比(19.4±2.5)ng/L](均P<0.01),罗红霉素组血清和BALF中RAGE、IL-4浓度均显著低于哮喘模型组[(438±18)比(494±32)ng/L、(22.8±6.0)比(32.4±5.8)ng/L和(444±42)比(553±38)ng/L、(25.6±4.5)比(37.8±3.4)ng/L](均P<0.05);哮喘模型组支气管壁增厚,管腔狭窄,黏膜皱折明显增多,黏膜下水肿,支气管、血管周围及肺泡腔内大量炎症细胞浸润、聚集;支气管壁厚度、平滑肌厚度明显厚于正常对照组(均P<0.01),罗红霉素组气道炎症及重塑改变优于哮喘模型组(P<0.05);哮喘模型组肺组织RAGE蛋白表达显著高于正常对照组(P<0.01),罗红霉素组肺组织RAGE蛋白表达显著低于哮喘模型组(P<0.01)。各组BALF及血清中RAGE和IL-4表达呈正相关(r=0.782、0.804,均P<0.01);肺组织中RAGE与白细胞总数、嗜酸粒细胞数量及平滑肌厚度呈正相关(r=0.897、0.927、0.860,均P<0.01)。结论哮喘大鼠肺组织、血清和BALF中RAGE表达升高,且与气道炎症及气道重塑呈正相关,罗红霉素可能通过降低哮喘大鼠RAGE的表达来达到治疗哮喘的目的。

关键词：哮喘糖基化终产物,高级罗红霉素大鼠

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：