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Proliferating cell nuclear antigen of Ulva prolifera is involved in the response to temperature stress

Journal of Oceanology and Limnology 2024年第4期42卷 1227-1241页

作者： Hongyan HE Juanjuan YANG Yuan HE Xiuwen YANG Caiwei FU Dongren ZHANG Jingwei DONG Aurang ZEB Jing QU songdong shen School of Biology&Basic Medical Sciences Soochow UniversitySuzhou 215101China Department of Pathology Affiliated Hospital and Medical School of Nantong UniversityNantong 226001China

Ulva prolifera is the most common specie causative to green tide,and its growth is sensitive to temperature ***,the mechanisms of *** response to temperature stress remain *** this study,high temperature(36℃)stimulus promoted the death of unformed cell wall protoplasts and delayed the division of formed cell wall protoplasts,while low-temperature(4℃)stimulus did not,suggesting that the mechanisms of the response of *** to high and low temperature stresses are *** results show that proliferation-related genes were differentially expressed under high and low-temperature stresses,especially the proliferating cell nuclear antigen(PCNA)and cyclins(CYCs).Subsequently,the interaction between PCNA and Cyclin A was confirmed by Co-immunoprecipitation,yeast two-hybrid,and so ***,high-and low temperature stresses induced the expression of PCNA and Cyclin A in varying of degrees,and activated extracellular signal-regulated kinase(ERK)signal *** results suggest,PCNA,Cyclin A,and ERK signal pathway played important roles in the resistance of *** to temperature ***,high-temperature stress induced an increase of miR-2916 in abundance,and exhibiting reverse expression of PCNA;and PCNA was target gene of miR-2916,suggesting that miR-2916 protected *** from high-temperature stress via post-transcriptionally regulation of *** study laid a foundation for understanding the function of PCNA and Cyclin A,moreover,it has a guiding significance to explore the mechanisms of the response to temperature stress from proliferation-related genes regulatory networks in ***.

关键词： UIva prolifera proliferating cell nuclear antigen Cyclin A extracellular signal-regulated kinase microRNA temperature stress

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Characterization of SARS-COV-2 main protease inhibitory peptides from Ulva prolifera proteins

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Journal of Oceanology and Limnology 2023年第5期41卷 1887-1899页

作者： Zhiyong LI Yehua WANG Caiwei FU Dongren ZHANG Tuanjie CHE songdong shen School of Biology&Basic Medical Sciences Soochow UniversitySuzhou 215101China Key Laboratory of Functional Genomic and Molecular Diagnosis of Gansu Province Lanzhou 730030China

The main protease(M^(pro))is essential for the replication of SARS-COV-2 and therefore represents a promising anti-viral *** this study,we screened M^(pro)inhibitory peptides from Ulva prolifera protein on in-silico *** analysis using the online toxic prediction tool ToxinPred revealed that all the peptides were *** hexapeptide(SSGFID)exhibited high M^(pro)inhibitory activity in molecular docking and its IC_(50)value was 139.40±0.82μmol/L in vitro according to fluorescence resonance energy transfer assay(FRET).Quantitative real-time(qRT-)PCR results show that SSGFID could stimulate the expression of mitosis-related factors,including nuclear factor-κB,cyclin D1,and cyclin-dependent kinase 4,to promote the proliferation of mice *** study revealed that SSGFID showed resistance against pepsin and trypsin but lost D(Asp)after pretreatment at121℃ for 15 ***,SSGFID was mainly transported through the Caco-2 cell monolayer by the peptide transporter PepT1 and passive-mediated transport during the transport ***,the peptide was also degraded by Caco-2 intracellular enzymes,and the transfer rate of intact peptide was4.2%.Furthermore,Lineweaver–Burk plots demonstrated that SSGFID possessed a mixed inhibitory characteristic with M^(pro).Our study indicated the potential of Ulva prolifera as antiviral and immuneenhancing functional food ingredients and nutraceuticals.

关键词： M^(pro)inhibitory peptide Ulva prolifera protein in-silico proteolysis immunomodulatory peptide gastrointestinal digestion transport study

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Study of screening,transport pathway,and vasodilation mechanisms on angiotensin-Ⅰconverting enzyme inhibitory peptide from Ulva prolifera proteins

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Acta Oceanologica Sinica 2023年第11期42卷 98-106页

作者： Zhiyong Li Yuan He Hongyan He Caiwei Fu Mengru Li Aiming Lu Dongren Zhang Tuanjie Che songdong shen School of Biology&Basic Medical Sciences Soochow UniversitySuzhou 215101China Key Laboratory of Functional Genomic and Molecular Diagnosis of Gansu Province Lanzhou 730030China

In this study,Ulva prolifera protein was used for preparing angiotensin-I converting enzyme(ACE)-inhibitory peptide via virtual gastrointestinal digestion and in silico *** parameters of the obtained peptide,such as inhibition kinetics,docking mechanism,stability,transport pathway,were explored by Lineweaver-Burk plots,molecular docking,in vitro stimulate gastrointestinal(GI)digestion and Caco-2 cells monolayer model,***,a novel anti-ACE peptide LDF(IC_(50),(1.66±0.34)μmol/L)was screened and synthesized by chemical *** was a no-competitive inhibitor and its anti-ACE inhibitory effect mainly attributable to four Conventional Hydrogen Bonds and Zn701 *** could keep activity during simulated GI digestion in vitro and was transported by peptide transporter PepT1 and passive-mediated ***,it could activate Endothelial nitric oxide synthase(eNOS)activity to promote the production of NO and reduce Endothelin-1(ET-1)secretion induced by AngiotensinⅡ(AngⅡ)in Human Umbilical Vein Endothelial Cells(HUVECs).Meanwhile,it could promote mice splenocytes proliferation in a concentration-dependent *** study indicated that this peptide was a potential ingredient functioning on vasodilation and enhancing immunity.

关键词： Ulva prolifera protein ACE-inhibitory peptide in vitro GI digestion vasodilation mechanism immunomodulation

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A New Molecular Label Applied to the Study of the Yellow Sea Green Tide

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Journal of Ocean University of China 2019年第6期18卷 1507-1514页

作者： shen Weijie HE Yuan shen songdong Department of Cell Biology College of Biology and Basic Medical Sciences Soochow University

From 2007 to 2017,large-scale green tides occurred every year in the Yellow Sea of China,and Ulva prolifera was the main species leading to the green *** this study,we used the Polymerase chain reaction and 3'Rapid-amplification of c DNA ends technique to amplify the nr DNA-LSU and IGS sequences in *** and one species of *** techniques showed 3259 bp of nrD NA-LSU and 3388 bp of IGS in *** and 3282 bp nr DNA-LSU and 3059 bp IGS in Blidingia *** the same time,tandem repeats,short dyads,palindromic and multiple simple repeat sequences in the IGS sequence were found by analyzing the structure of the IGS sequence in *** and Blidingia sp.G+C contents of the IGS sequence in *** and Blidingia *** 52.42% and 53.09%,*** divided the *** into two types according to the morphological *** the specimens of *** from the Qingdao coastal area,Jiangsu coastal area and the Yellow Sea have different morphologies,their ITS and IGS sequences are almost ***,the main species of the green tides in the Qingdao coastal area,Jiangsu coastal area and the Yellow Sea are the same and have the same origin.

关键词： LSU IGS Ulva prolifera green tide morphological characteristics

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Effects of light and salinity on carotenoid biosynthesis in Ulva prolifera

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Acta Oceanologica Sinica 2020年第10期39卷 50-57页

作者： Yuan He Yuantu Ye songdong shen College of Biology and Basic Medical Sciences Soochow UniversitySuzhou 215123China

Ulva prolifera is a green alga that plays an important role in green *** biosynthesis is a basic terpenoid metabolism that is very important for maintaining normal life activities in *** this study,we first reported the complete sequences of all genes in the 2-C-methyl-D-erythritol 4-phosphate(MEP)pathway,which is the only carotenoid synthesis pathway in ***,we compared these genes with those of other ***,by detecting the carotenoid contents and expression levels of key genes participating in carotenoid biosynthesis in *** under three different light(1000 lx,5000 lx and 12000 lx)and salinity(12,24 and 40)regimes,we found that carotenoid synthesis could be influenced by light and salinity,such that low light and high salinity could promote the synthesis of *** results showed that the expression levels of genes involved in the MEP and the downstream pathway could affect the biosynthesis of carotenoids at the molecular *** study contributes to a better understanding of the roles of genes participating in carotenoid biosynthesis in *** and the environmental regulation of these genes.

关键词： U.prolifera carotenoid MEP pathway light salinity

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Expression analyses of miRNA Up-MIR-843 and its target genes in Ulva prolifera

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Acta Oceanologica Sinica 2020年第10期39卷 27-34页

作者： Juanjuan Yang Dachun Yu songdong shen Department of Cell Biology School of Biology and Basic MedicalSoochow UniversitySuzhou 215123China

microRNAs(miRNA)families play a critical role in plant growth,development,and responses to abiotic *** this study,we characterized Up-miR-843 and its targets genes in Ulva prolifera responses to nitrogen depravation and heat *** data demonstrated that 184 target genes of Up-miR-843 could be successfully validated.N deficiency not heat stress stimulus induced increase in abundance of the Up-miR-843 while exhibited reverse expression of target genes,including cyclin A3 and cyclin L,which were strictly required for cell cycle *** addition,*** with highly expression of Up-miR-843 showed improved biomass,and photosynthesis compared with that under normal growth ***,the N deprivation and heat responsive miRNAs might be a possible member mediating the expression of these target genes,which further regulated the growth of ***.

关键词： cyclins nitrogen deprivation microRNA U.prolifera heat stress

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Effects of Methyl Jasmonate on the Composition of Volatile Compounds in Pyropia yezoensis

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Journal of Ocean University of China 2018年第2期17卷 291-296页

作者： HE Lihong WANG Liang WANG Linfang shen songdong Department of Cell Biology Medical College of Soochow UniversitySuzhou 215006China Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological SciencesChinese Academy of SciencesShanghai 200031China Shanxi Academy of Environmental Research Taiyuan 030027China

Volatile organic compounds in marine algae have been reported to comprise characteristic flavor of algae and play an important role in their growth, development and defensive response. Yet their biogeneration remain largely unknown. Here we studied the composition of volatile compouds in Pyropia yezoensis and their variations in response to methyl jasmonate(MeJA) and diethyldithiocarbamic acid(DIECA) treatment using gas chromatography-mass spectrometry(GC-MS). A total of 44 compounds belonging to the following chemical classes(n) were identified, including aldehydes(11), alcohols(8), acids and esters(6), alkanes(5), ketones(5), alkenes(3), and S-or N-containing miscellaneous compounds(6). External treatment with plant hormone MeJA increased the content of 1-dodecanol, 4-heptenal, and 2-propenoic acid-2-methyl dodecylester, but decreased the content of phytol, 3-heptadecene, 2-pentadecanone, and isophytol. When pretreated with DIECA, an inhibitor of the octadecanoid pathway leading to the biosynthesis of endogeneous jasmonates and some secondary metabolites, phytol and isophytol were increased, while 4-heptenal, 1-dodecanol, and 2-propenoic acid-2-methyl dodecylester were decreased, both of which were negatively correlated with their variations under MeJA treatment. Collectively, these results suggest that MeJA does affect the volatile composition of P. yezoensis, and the octadecanoid pathway together with endogenous jasmonate pathway may be involved in the biosynthesis of volatile compounds, thereby providing some preliminary envision on the composition and biogeneration of volatile compounds in P. yezoensis.

关键词： Pyropia yezoensis methyl jasmonate volatile compounds flavor

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Reproduction Diversity of Enteromorpha prolifera

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Journal of Integrative Plant Biology 2008年第5期50卷 622-629页

作者： Apeng Lin songdong shen Jianwei Wang Binlun Yan Life Sciences School Soochow University Suzhou 215123 China Marine Biotechnology Key Construction Laboratory Huaihai Institute of Technology Lianyungang 226001 China

Enteromorpha prolifera （Muell.） J. Agardh （Chlorophyta, Ulvophyceae）. which is distributed widely in the inter-tidal zone of the ocean, is one of the most common fouling green algae. However, the present understandings of the life history of E. profifera have been insufficient to explain their seasonal abundances. Thus it is essential to investigate how many reproductive strategies are likely to contribute to the successful colonization and flourishing of the green alga. In the present study the reproduction diversity of E. prolifera was observed and studied systematically by culturing chopped tissues. Our results showed that there are in total seven pathways of reproduction for E. prolifera including sexual, asexual and vegetative reproduction, it was indicated that the variety of the reproductive ways and the large quantity of reproductive cells produced and released during the reproductive season are the two key factors that facilitate colonization of E. prolifera. The reproduction of the alga E. prolifera mainly depends on asexual methods. The results presented here contribute to increasing our understanding about how the opportunistic macroalgae successfully maintain colonization and excessive growth.

关键词： colonization Life history macroalgal blooms propagation tissue culture.

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The Ulva prolifera genome reveals the mechanism of green tides

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Journal of Oceanology and Limnology 2021年第4期39卷 1458-1470页

作者： Yuan HE songdong shen Dachun YU Yehua WANG Jiao YIN Zongling WANG Yuantu YE Institute of Aquaculture College of Biology and Basic Medical SciencesSoochow UniversitySuzhou 215123China First Institute of Oceanography(FIO) Ministry of Natural Resources(MNR)Qingdao 266100China

The genome of green microalgae has rarely been *** prolifera is a green microalga that has received much *** research articles about *** in recent years,we know very little about its ***,the 87.9-Mb haploid genome(containing 10311 protein-coding genes)of *** was studied,and the genome was compared with that of ***,which is the only published Ulva *** showed that the two species are closely related.A phylogenetic tree was constructed among *** and other green algae available in GenBank,revealing the evolutionary status of *** in *** understand why *** could grow rapidly,we identified some genes related to growth,such as those involved in cell division,phosphorylation,and cell *** addition,genes related to stress resistance were found,which supports the notion that *** grows vigorously in *** results help to characterize green tides from a new perspective and reveal some important insight into the biology of ***.

关键词： Ulva prolifera genome fast grow evolution

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Comparison of Chlorophyll and Photosynthesis Parameters of Floating and Attached Ulva prolifera

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Journal of Integrative Plant Biology 2011年第1期53卷 25-34页

作者： Apeng Lin songdong shen Guangce Wang Qianqian Yi Hongjin Qiao Jianfeng Niu Guanghua Pan Key Laboratory of Experimental Marine Biology Institute of Oceanology the Chinese Academy of Sciences Qingdao 266071 China Graduate University of Chinese Academy of Sciences Beijing 100049 China Department of Cell Biology Medical College of Soochow University Suzhou 215123 China College of Marine Science and Engineering Tianjin University of Science & Technology Tianjin 300457 China

In mid-May 2008 a serious green tide caused mainly by floating Ulva prolifera （Mvller） J. Agardh （Chlorophyta, Ulvales） thalli struck the coastal area of Qingdao, China. To understand the present physiological conditions of the floating alga, in this work both laboratory and field investigations were conducted on the floating U. prolifera thalli in comparison with the attached U. prolifera thalli collected from the area. The floating thalli of three distinctively different colors and attached thalli at three different stages of sporangium formation process were characterized under a microscope, while their photosynthetic parameters were determined with chlorophyll fluorescence technology. On the other hand, the sporangium formation status of the floating U. prolifera thalli was surveyed both in the laboratory and in the field. Comparisons showed that both of the paired morphological characteristics and the paired physiological parameters of the floating and attached U. prolifera thalli were consistent. Furthermore, some spores were confirmed in the field and some motile particles were found within the floating thalli. These results suggest that the floating U. prolifera thalli with different colors could be at different stages of sporangium formation. However, our results also showed that the floating alga thalli have only a limited reproductive potential. This might limit the duration and the further geographic expansion of the green algal bloom.

关键词： Greening Algae Fields Colour Laboratories

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