Study of screening,transport pathway,and vasodilation mechanisms on angiotensin-Ⅰconverting enzyme inhibitory peptide from Ulva prolifera proteins

作     者：Zhiyong Li Yuan He Hongyan He Caiwei Fu Mengru Li Aiming Lu Dongren Zhang Tuanjie Che Songdong Shen Zhiyong Li;Yuan He;Hongyan He;Caiwei Fu;Mengru Li;Aiming Lu;Dongren Zhang;Tuanjie Che;Songdong Shen

作者机构：School of Biology&Basic Medical SciencesSoochow UniversitySuzhou 215101China Key Laboratory of Functional Genomic and Molecular Diagnosis of Gansu ProvinceLanzhou 730030China 

出 版 物：《Acta Oceanologica Sinica》 (海洋学报（英文版）)

年 卷 期：2023年第42卷第11期

页      面：98-106页

核心收录：

学科分类：1008[医学-中药学(可授医学、理学学位)] 1006[医学-中西医结合] 100602[医学-中西医结合临床] 10[医学] 

主　　题：Ulva prolifera protein ACE-inhibitory peptide in vitro GI digestion vasodilation mechanism immunomodulation 

摘      要：In this study,Ulva prolifera protein was used for preparing angiotensin-I converting enzyme(ACE)-inhibitory peptide via virtual gastrointestinal digestion and in silico *** parameters of the obtained peptide,such as inhibition kinetics,docking mechanism,stability,transport pathway,were explored by Lineweaver-Burk plots,molecular docking,in vitro stimulate gastrointestinal(GI)digestion and Caco-2 cells monolayer model,***,a novel anti-ACE peptide LDF(IC_(50),(1.66±0.34)μmol/L)was screened and synthesized by chemical *** was a no-competitive inhibitor and its anti-ACE inhibitory effect mainly attributable to four Conventional Hydrogen Bonds and Zn701 *** could keep activity during simulated GI digestion in vitro and was transported by peptide transporter PepT1 and passive-mediated ***,it could activate Endothelial nitric oxide synthase(eNOS)activity to promote the production of NO and reduce Endothelin-1(ET-1)secretion induced by AngiotensinⅡ(AngⅡ)in Human Umbilical Vein Endothelial Cells(HUVECs).Meanwhile,it could promote mice splenocytes proliferation in a concentration-dependent *** study indicated that this peptide was a potential ingredient functioning on vasodilation and enhancing immunity.