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Natural variation in maize gene ZmSBR1 confers seedling resistance to Fusarium verticillioides

The Crop Journal 2024年第3期12卷 836-844页

作者： Yunxia song Peipei Ma Jingyang Gao Chaopei Dong Zhao Wang Yifan Luan Jiafa Chen Doudou Sun Pei Jing Xuecai Zhang weibin song Zijian Zhou Jianyu Wu College of Agronomy Henan Agricultural UniversityZhengzhou 450002HenanChina College of Life Sciences Henan Agricultural UniversityZhengzhou 450002HenanChina Global Maize Program International Maize and Wheat Improvement Center(CIMMYT)Apdo 6-64106600 MexicoDFMexico National Maize Improvement Center Department of Plant Genetics and BreedingChina Agricultural UniversityBeijing 100193China

Maize seedling blight caused by Fusarium verticillioides is a widely occurring maize disease,but the genetics and mechanisms of resistance are not well *** this study,GWAS performed by MLM and 3VmrMLM identified 40 and 20 QTNs,associated with seedling blight *** methods identified 49 and 36 genes,*** verification of candidate gene ZmSBR1 identified by both methods showed that the resistance of a mutant line to seedling blight decreased by 0.37 grade points after inoculation with ***,compared with the *** length of the stem rot lesion caused by *** increased by 86%in mutant seedlings,and the relative length of the adult plant stalk rot increased by 35%in mutant plants compared to the wild type after inoculation with Fusarium *** analysis showed that expression of defense-related genes after inoculation was down-regulated in the mutant compared to the wild type,synthesis of secondary metabolites associated with resistance was reduced,and the immune response triggered by PAMP decreased,resulting in decreased resistance of mutant maize *** gene association analysis showed that most maize inbred lines carried the susceptible haplotype.A functional PCR marker was *** results demonstrated that ZmSBR1 conferred resistance to multiple Fusarium diseases at the seedling and adult growth stages and had important application value in breeding.

关键词： Seedling blight Stalk rot Multiple disease resistance

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Application of CRISPR/Cas12i.3 for targeted mutagenesis in broomcorn millet(Panicum miliaceum L.)

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Journal of Integrative Plant Biology 2024年第8期66卷 1544-1547页

作者： Yuhe Bai Shengnan Liu Yan Bai Zhisong Xu Hainan Zhao Haiming Zhao Jinsheng Lai Ya Liu weibin song State Key Laboratory of Maize Bio-breeding Key Laboratory of Genome Editing Research and ApplicationMinistry of Agriculture and Rural AffairsDepartment of Plant Genetics and BreedingNational Maize Improvement CenterCollege of Agronomy and BiotechnologyChina Agricultural UniversityBeijing 100193China National Agricultural Technology Extension&Service Center Beijing 100125China Beijing Key Laboratory of Maize DNA Fingerprinting and Molecular Breeding Beijing Academy of Agriculture and Forestry SciencesBeijing 100097China

As the global human population continues to grow and is predicted to reach 10 billion by 2050,the demand for food volume and quality raises a tremendous challenge(OECD/FAO,2017).The worldwide arable land area is decre... 详细信息

关键词： arable continue raise

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四线叠加超深群坑施工关键技术研究

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建筑施工 2024年第8期46卷 1324-1330页

作者：宋为宾上海建工二建集团有限公司上海200080 上海建筑工程逆作法工程技术研究中心上海200080

为了缓解日益拥堵的交通情况,由地下轨道交通、地下快速道路和地面道路组成的多线叠加的地下交通枢纽系统应运而生。同时随着基坑深度的增加,地下连续墙进入更深的土层,对施工技术和施工装备形成了全新挑战。通过上海轨道交通14号线、1... 详细信息

为了缓解日益拥堵的交通情况,由地下轨道交通、地下快速道路和地面道路组成的多线叠加的地下交通枢纽系统应运而生。同时随着基坑深度的增加,地下连续墙进入更深的土层,对施工技术和施工装备形成了全新挑战。通过上海轨道交通14号线、18号线昌邑路车站工程案例,对四线叠加超深群坑施工过程中的分坑施工、超深地下连续墙施工、封堵墙拆除施工、地下水控制等施工技术进行了分析,提出软硬复合地层地下连续墙“抓铣结合”施工工艺、66 m钢筋笼“分节吊装”的施工思路,还进一步研究了“双帷幕”止水体系在复杂水文条件下对超深基坑周边环境的保护性。结果表明,本项目地下连续墙的垂直度表现良好,地下连续墙钢筋笼均一次成形、一次入槽、一次浇筑完成、无明显渗漏点,同时基坑开挖及上撑速度加快,地下水也得到了良好的控制,可为同类型地下交通枢纽工程提供参考。

关键词：地下交通枢纽分坑施工抓铣结合分节吊装 “双帷幕”止水体系

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基于CESE方法的煤矿风井泄爆全过程模拟与消波增效研究

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煤炭科学技术 2024年第5期52卷 127-138页

作者：宋维宾孙玉宁王永龙王志明河南理工大学能源科学与工程学院河南焦作454003 煤炭安全生产与清洁高效利用省部共建协同创新中心河南焦作454003

为揭示煤矿风井泄爆过程、探寻增强泄爆效果方法,针对现行泄爆方法和多种改进泄爆方法,建立了系列全尺寸三维仿真模型,利用LS−DYNA软件的CESE求解器,进行了全过程流固耦合模拟分析。结果表明:现行防爆门在泄爆过程中会引发较强烈的反射... 详细信息

为揭示煤矿风井泄爆过程、探寻增强泄爆效果方法,针对现行泄爆方法和多种改进泄爆方法,建立了系列全尺寸三维仿真模型,利用LS−DYNA软件的CESE求解器,进行了全过程流固耦合模拟分析。结果表明:现行防爆门在泄爆过程中会引发较强烈的反射冲击波且不能快速有效地予以消弱,致使风硐中先后出现可对风机造成二次冲击的2道冲击波;去除防爆门立壁结构对提升泄爆效果作用不明显,但可使防爆门受到的冲击明显减弱;在一定范围内,减轻防爆门质量对提高泄爆效果的作用较为有限,且会使防爆门吸收的爆炸能量明显增加;在增量不大的情况下,增大防爆门到风井和风硐交岔点的距离即能有效改善泄爆效果;侧向和正向先行泄爆方法均能明显增强泄爆效果,并对防爆门有显著的减冲和保护作用,在算例条件下,最优可使反射波超压峰值下降49.4%和28.3%;防爆门开启时间、泄爆面积和防爆门到风井/风硐交岔点的距离是影响泄爆效果的重要因素;风井达到良好泄爆效果所需要的开启时间比现行防爆门要短得多;仅在井口设置防爆门存在不能消减风硐中第1道冲击波超压峰值的局限性。基于对风井泄爆过程、机理和方法的新认识,提出了以“两区域多通道”泄爆为特征的主辅防爆门协同泄爆方法,以系统提升风井泄爆效果和防爆水平。

关键词：风井煤矿风井泄爆先行泄爆协同泄爆防爆门流固耦合

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坑外降水-回灌技术结合在复杂工况超深基坑工程中的应用研究

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建筑施工 2024年第6期46卷 953-956页

作者：宋为宾上海建工二建集团有限公司上海200080 上海建筑工程逆作法工程技术研究中心上海200080

在深基坑内降承压水将导致地下连续墙内外侧出现水头差,易使地下连续墙的薄弱部位产生渗漏,甚至涌水涌沙。采用基坑外管井降水减小水头差是较为经济有效的措施,同时采取双止水帷幕和回灌措施可保护基坑周边建筑物,达到既保证基坑安全施... 详细信息

在深基坑内降承压水将导致地下连续墙内外侧出现水头差,易使地下连续墙的薄弱部位产生渗漏,甚至涌水涌沙。采用基坑外管井降水减小水头差是较为经济有效的措施,同时采取双止水帷幕和回灌措施可保护基坑周边建筑物,达到既保证基坑安全施工又能减少对周边环境影响的效果。以某地铁车站为工程背景,介绍了Y线车站主体基坑施工过程中,采取基坑外双止水帷幕内降水和回灌技术相结合的方式,降低因深基坑施工降承压水对周边环境的不利影响,为同类型工程提供经验借鉴。

关键词：基坑外降水回灌技术双止水帷幕地下连续墙建筑物沉降

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基于多源数据的中美作物种质资源管理的比较及启示

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中国农业大学学报 2024年第10期29卷 161-171页

作者：李红军孟晨霞张红伟宋伟彬刘兴旺中国农业大学图书馆北京100083 中国农业大学农学院北京100193 中国农业大学园艺学院北京100193

为剖析我国作物种质资源管理存在的问题、提出其利用率提升的可行性路径,查询和分析2014-2023年Web of Science数据库的相关论文以及中美两国政府和官方机构网站中的制度政策、规划方案等文献。结果表明:1)我国(不包括台湾地区省数据)... 详细信息

为剖析我国作物种质资源管理存在的问题、提出其利用率提升的可行性路径,查询和分析2014-2023年Web of Science数据库的相关论文以及中美两国政府和官方机构网站中的制度政策、规划方案等文献。结果表明:1)我国(不包括台湾地区省数据)和美国的作物种质资源管理分别采取垂直化和扁平化的管理方式,各有优缺点;2)我国作物种质资源保存数量接近美国,但在表型鉴定、基因型鉴定和利用率方面差距较大;3)我国作物种质资源规划中存在的滞后国际形势和科技态势之处,未能及时更新;4)我国作物种质资源管理的信息化程度低于美国,不利于材料的查询和获取;5)我国作物种质资源的研究对新兴技术的响应不如美国积极;6)我国作物种质资源的国际合作广度低于美国。因此,建议我国的作物种质资源管理做以下调整:1)积极应对国际形势和科技发展的变化,及时调整规划目标;2)提高信息化程度,方便查询和使用资源;3)提高知识产权保护意识,防止种质资源境外流失;4)扩大国际合作渠道,提升我国作物种质资源利用的国际合作影响力和主导力。

关键词：作物种质资源中国美国多源数据文献计量

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Efficiency and Inheritance of Targeted Mutagenesis in Maize Using CRISPR-Cas9

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Journal of Genetics and Genomics 2016年第1期43卷 25-36页

作者： Jinjie Zhu Ning song Silong Sun Weilong Yang Haiming Zhao weibin song Jinsheng Lai State Key Laboratory of Agrobiotechnology and National Maize Improvement Center Department of Plant Genetics and Breeding China Agricultural University

CRISPR/Cas （clustered regularly interspaced short palindromic repeats/CR1SPR-associated proteins） is an adaptive immune system in bacteria and archaea to defend against invasion from foreign DNA fragments. Recently, it has been developed as a powerful targeted genome editing tool for a wide variety of species. However, its application in maize has only been tested with transiently expressed somatic cells or with a limited number of stable transgenic To plants. The exact efficiency and specificity of the CRISPR/Cas system in the highly complex maize genome has not been documented yet. Here we report an extensive study of the well-studied type II CRISPR-Cas9 system for targeted genome editing in maize, with the codon-optimized Cas9 protein and the short non-coding guide RNA generated through a functional maize U6 snRNA promoter. Targeted gene mutagenesis was detected for 90 loci by maize protoplast assay, with an average cleavage efficiency of 10.67 %. Stable knockout transformants for maize phytoene synthase gene （PSYI） were obtained. Mutations occurred in germ ceils can be stably inherited to the next generation. Moreover, no off-target effect was detected at the computationally predicted putative off-target loci. No significant difference between the transcriptomes of the Cas9 expressed and non-expressed lines was detected. Our results confirmed that the CRISPR-Cas9 could be successfully applied as a robust targeted genome editing system in maize.

关键词： CRISPR-Cas9 Targeted mutagenesis Heritability Maize

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Genetic dissection of maize seedling root system architecture traits using an ultra-high density bin-map and a recombinant inbred line population

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Journal of Integrative Plant Biology 2016年第3期58卷 266-279页

作者： weibin song Baobao Wang Andrew L Hauck Xiaomei Dong Jieping Li Jinsheng Lai State Key Laboratory of Agrobiotechnology and National Maize Improvement Center of China Department of Plant Genetics and BreedingChina Agricultural University

Maize（Zea mays） root system architecture（RSA）mediates the key functions of plant anchorage and acquisition of nutrients and water. In this study,a set of 204 recombinant inbred lines（RILs） was derived from the widely adapted Chinese hybrid ZD958（Zheng58 Chang7-2）,genotyped by sequencing（GBS） and evaluated as seedlings for 24 RSA related traits divided into primary,seminal and total root classes. Signi ficant differences between the means of the parental phenotypes were detected for 18 traits,and extensive transgressive segregation in the RIL population was observed for all traits. Moderate to strong relationships among the traits were discovered. A total of 62 quantitative trait loci（QTL） were identi fied that individually explained from1.6% to 11.6%（total root dry weight/total seedling shoot dry weight） of the phenotypic variation. Eighteen,24 and 20 QTL were identi fied for primary,seminal and total root classes of traits,respectively. We found hotspots of 5,3,4 and 12 QTL in maize chromosome bins 2.06,3.02-03,9.02-04,and 9.05-06,respectively,implicating the presence of root gene clusters or pleiotropic effects. These results characterized the phenotypic variation and genetic architecture of seedling RSA in a population derived from a successful maize hybrid.

关键词： Maize root system architecture QTL bin map genotyping by sequencing(GBS)

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MP3RNA-seq:Massively parallel 3’end RNA sequencing for high-throughput gene expression profiling and genotyping

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Journal of Integrative Plant Biology 2021年第7期63卷 1227-1239页

作者： Jian Chen Xiangbo Zhang Fei Yi Xiang Gao weibin song Haiming Zhao Jinsheng Lai State Key Laboratory of Plant Physiology and Biochemistry National Maize Improvement CenterDepartment of Plant Genetics and BreedingChina Agricultural UniversityBeijing 100193China Center for Crop Functional Genomics and Molecular Breeding China Agricultural UniversityBeijing 100193China

Transcriptome deep sequencing(RNA‐seq)hasbecome a routine method for global geneexpression ***,its application tolarge‐scale experiments remains limited by costand labor *** we describe amassively parallel 3′end RNA‐seq(MP3RNA‐seq)method that introduces unique samplebarcodes during reverse transcription to permitsample pooling immediately following this ***3RNA‐seq allows for handling of hun-dreds of samples in a single experiment,at acost of about$6 per sample for libraryconstruction and ***3RNA‐seq iseffective for not only high‐throughput geneexpression profiling,but also *** its utility,we applied MP3RNA‐seqto 477 double haploid lines of *** iden-tified 19,429 genes expressed in at least 50%ofthe lines and 35,836 high‐quality singlenucleotide polymorphisms for *** with these data,we performedexpression and agronomic trait quantitativetrait locus(QTL)mapping and identified 25,797expression QTLs for 15,335 genes and 21 QTLsfor plant height,ear height,and relative *** conclude that MP3RNA‐seq is highlyreproducible,accurate,and sensitive forhigh‐throughput gene expression profiling andgenotyping,and should be generally applicableto most eukaryotic species.

关键词： gene expression profiling genotyping maize MP3RNA‐seq quantitative trait loci

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Molecular mapping and candidate gene analysis of the semi-dominant gene Vestigial glume1 in maize

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The Crop Journal 2019年第5期7卷 667-676页

作者： Chaoxian Liu Yuanzeng Zhao Yang Bai Xiaomin Lu weibin song Liping Qin Yilin Cai Maize Research Institute Southwest UniversityChongqing 400715China Henan Institute of Science and Technology Xinxiang 453003HenanChina Henan Academy of Agricultural Sciences Zhengzhou 450002HenanChina National Maize Improvement Center of China China Agricultural UniversityBeijing 100193China

The glume is an organ of the maize spikelet and plays important roles in anther and kernel *** glume1(Vg1)is a classic mutant associated with ligule and glume *** we report the phenotypic characterization,fine mapping,and candidate gene analysis of the Vg1 ***1 is a semi-dominant and pleiotropic gene,and also affects plant height,ear height,and tassel ***1 ligule degeneration begins at the first leaf,and the Vg1 tassel and ear can be distinguished from those of wild-type plants when their lengths reach respectively 55 mm and 51 *** a BC3 mapping population of 11,445 plants,we delimited the Vg1 functional site to an interval of 7.4 kb,flanked by the markers InDelLM and CRM6.A putative cyclopropane fatty-acid synthase gene(ZmCPA-FAS1)was hypothesized to underlie the mutant *** detected a Helitron insertion in the sixth intron of *** presence caused abnormal alternative splicing of ZmCPA-FAS1 that conferred new characteristics on the Vg1 *** findings are a basis for further discovery of the molecularmechanism underlying glume development and a potential guide formaize breeding of small-glume varieties,especially sweet corn breeding.

关键词： Vestigial glume1 Fine mapping Candidate gene analysis ZmCPA-FAS1 Glume development

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