检索结果-南通市图书馆

Alpha-tocopherol supplementation on chromium toxicity: a study on rat liver and kidney cell membrane

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Journal of Environmental Sciences 2003年第3期15卷 356-359页

作者： Sankar Kumar Dey Prasunpriya Nayak somenath roy Department of Human Physiology with Community Health Vidyasagar UniversityDepartment of Human Physiology with Community Health Vidyasagar UniversityDepartment of Human Physiology with Community Health Vidyasagar University Midnapore-721 102 West Bengal IndiaMidnapore-721 102 West Bengal IndiaMidnapore-721 102 West Bengal India

Membrane damage is one of the important consequence of chromium, an environmental toxicant, to produce cytotoxicity. α-tocopherol, a membrane protectant can be used to reduce the chromium-induced membrane damage. In the present study, the impact of chromium in presence and absence of α-tocopherol was studied on plasma membrane of liver and kidney in male Wistar rats (80—100 g body weight). Significant increase in membrane cholesterol level as well as significant decrease in membrane phospholipid level in chromium exposed ( 0.8 mg /100 g body weight/d, i.p., for 4 weeks) animals suggest structural alteration of both liver and kidney plasma memebrane. The alkaline phosphatase, total ATPase and Na +-K +-ATPase activities of plasma membrane were significantly decreased in both liver and kidney after chromium treatment. However, α-tocopherol (30 mg/100 g diet) supplementation can restrict the changes in these membrane-bound enzyme activities. Thus, the usefulness of dietary supplementation of α-tocopherol to restrain the chromium-induced membrane damage is suggested.

关键词： chromium liver kidney membrane α-tocopherol

A review of age-old antimalarial drug to combat malaria:efficacy upgradation by nanotechnology based drug delivery

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Asian Pacific Journal of Tropical Medicine 2014年第9期7卷 673-679页

作者： Satyajit Tripathy somenath roy Immunology and Microbiology Laboratory Department of Human Physiology with Community HealthVidyasagar UniversityMidnapore-721102West BengalIndia

Malaria is uncontrolled burden in the world till now.Despite of different efforts to develop antimalarial drug for decades,any anti-malarial drug can able to eradicate completely till now.Many anti-malarial substances are practically ineffectual because of their physicochemical limitations,cytotoxicity,chemical instability and degradation,and limited activities against intracellular parasites.Taking into consideration,the amount of research is going to conduct in the field of nanoparticle based drug delivery systems,lead to new ways of improving the treatment of infectious diseases.The study has focused on the progress and advancement of research on nanotechnology based drug delivery to eradicate the malaria.We like to focus the efficacy of nanotechnology based drug application for the opening out of novel chemotherapeutics in laboratory research,which may show the way to better use with age-old antimalarial drug and may draw the attention of pharmaceutical industries for the improvement and designing of effective anti-malarial drugs in future.

关键词： Nanotechnology Age-old drug Drug efficacy Antimalaria Nanomedicine

Aqueous extract of Ocimum gratissimum Linn and ascorbic acid ameliorate nicotine-induced cellular damage in murine peritoneal macrophage

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Asian Pacific Journal of Tropical Medicine 2010年第10期3卷 775-782页

作者： Santanu Kar Mahapatra Subhankari Prasad Chakraborty somenath roy Immunology and Microbiology Laboratory Department of Human Physiology with Community HealthVidyasagar UniversityMidnapore -721 102West BengalIndia

Objective:To test the in vitro protective role of aqueous extract of Ocimum gratissimum Linn. (0.gratissimum) and ascorbic acid against nicotine-induced murine peritoneal macrophage. Methods:Peritoneal macrophages from mice were treated with nicotine(10 mM),nicotine (10 mM) with aqueous extract of O.gratissimum(1 to 25μg/mL),and nicotine(10 mM) with ascorbic acid(0.01 mM) for 12 h in cell culture media,while the control group was treated with culture media.Levels of free radical generation,lipid peroxidation,protein carbonyls,oxidized glutathione levels and DNA damage were observed and compared.Results:Phytochemical analysis of aqueous extract has shown high amount of phenolics and flavonoids compound present in it.The significantly increased free radical generation,lipid peroxidation,protein carbonyls,oxidized glutathione levels and DNA damage were observed in nicotine-treated group as compared to the control group:those were significantly reduced in aqueous extract of O. gratissimum and ascorbic acid supplemented groups.Moreover,significantly reduced antioxidant status in nicotine exposed murine peritoneal macrophage was effectively ameliorated by these two products.Among the different concentration of aqueous extract of O.gratissimum,the maximum protective effect was observed at 10μg/mL which does not produce any significant change in the normal cell.Conclusions:These findings suggest the potential use and beneficial role of O.gratissimum as a modulator of nicotine-induced cellular damage in murine peritoneal macrophage.

关键词： Ocimum gratissimum Linn Nicotine Peritoneal macrophage Free radical DNA fragmentation

Biochemical characters and antibiotic susceptibility of Staphylococcus aureus isolates

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Asian Pacific Journal of Tropical Biomedicine 2011年第3期1卷 212-216页

作者： Subhankari Prasad Chakraborty Santanu Kar Mahapatra somenath roy Immunology and Microbiology Laboratory Department of Human Physiology with Community HealthVidyasagar University

Objective:To observe the biochemical characters and antibiotic susceptibility of isolated Staphylococcia aureus(S.auerus) strains against some conventional and traditional antibiotics. Methods:Thirty post operative pathogenic isolated S.aureus strains were used in this study. Bacterial culture was done in Mueller-Hinton broth at 37 ℃.Characters of these strains were determined by traditional biochemical tests such as hydrolysis test of gelatin,urea,galactose, starch and protein,and fermentation of lactose and sucrose.Antibiotic susceptibility were carried out by minimum inhibilory concentration test,minium bactericidal concentration test,disc agar diffusion test and brain heart infusion oxacillin screening agar.Results:Prom this study,it was observed that 100%S.aureus isolates showed positive results in gelatin,urea and galactose hydrolysis test.50%isolates were positive in starch hydrolysis test,35%in protein hydrolysis test. 100%isolates in lactose fermenting test,but no isolate was positive in sucrose fermenting test. Antibiotic susceptibility testing suggested that 20%of isolates were resistant to kanamycin and 46.67%were resistant to oxacillin.Conclusions:These findings show that all these isolates have gelatin,urea,galactose hydrolysis and lactose fermenting activity.20%of these isolates were resistant to kanamvcin and 46.67%were resistant to oxacillin.

关键词： Staphylococcus aureus Oxacillin Antibiotic susceptibility Minimum inhibitory concentration Disc agar diffusion Pathogen Biochemical character Bacterial culture

Alteration of some cellular function in amikacin resistant Pseudomonas aeruginosa transfected macrophages:a time dependent approach

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Asian Pacific Journal of Tropical Biomedicine 2011年第6期1卷 482-487页

作者： Subhankari Prasad Chakraborty Santanu KarMahapatra Sabyasachi Das somenath roy Immunology and Microbiology Laboratory Department of Human Physiology with Community HealthVidyasagar University

Objective:To evaluate the free radical generation and antioxidant enzymes status in murine peritoneal macrophage during in vitro amikacin resistant Pseudomonas aeruginosa(ARPA) treatment with different time interval.Methods:Peritoneal macrophages were treated with 1× 10~2 CFU/mL ARPA cell suspension in vitro for different time interval(1,2,3,6,12,and 24 h) and super oxide anion generation,NO generation,reduced glutathione level and antioxidant enzymes status were analyzed.Results:Super oxide anion generation and NO generation got peak at 12 h,indicating maximal free radical generation through activation of NADPH oxidase in murine peritoneal macrophages during ARPA transfection.Reduced glutathione level and antioxidant enzymes status were decreased significantly(P<0.05) with increasing time of ARPA transfection. All the changes in peritoneal macrophages after 12 h in vitro ARPA transfection had significant difference(P<0.05).Conclusions:From this study,it may be summarized that in vitro ARPA infection not only generates excess free radical but also affects the antioxidant system and glutathione cycle in murine peritoneal macrophage.

关键词： Antioxidant enzyme ARPA Oxidative stress Peritoneal macrophage Free radical generation

Biocompatibility of folate-modified chitosan nanoparticles

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Asian Pacific Journal of Tropical Biomedicine 2012年第3期2卷 215-219页

作者： Subhankari Prasad Chakraborty Sumanta Kumar Sahu Panchanan Pramanik somenath roy Immunology and Microbiology Laboratory Department of Human Physiology with Community HealthVidyasagar University Nanomaterials Laboratory Department of ChemistryIndian Institute of TechnologyKharagpur

Objective:To evaluate the acute toxicity of carboxymethyl chitosan-2,2' ethylenedioxy bisethylamine-folate(CMC-EDBE-FA) and as well as possible effect on microbial growth and in vitro cell cyto-toxicity.Methods:CMC-EDBE-FA was prepared on basis of carboxymethyl chitosan tagged with folic acid by covalently linkage through 2,2' elhylenedioxy bis-ethylamine. In vivo acute toxicity,in vitro cyto-toxicity and antimicrobial activity of CMC-EDBE-FA nanoparticle were determined.Results:Vancomycin exhibited the antibacterial activity against vancomycin sensitive Staphylococcus aureus,but CMC-EDBE-FA nanoparticle did not give any antibacterial activity as evidenced by minimal inhibitory concentration(MIC),minimal bactericidal concentration(MBC),disc agar diffusion(DAD) and killing kinetic assay.Further,the CMC-EDBE-FA nanoparticle showed no signs of in vivo acute toxicity up to a dose level of 1000 mg/kg p.o.,and as well as in vitro cyto-toxicity up to 230 μg/mL.Conclnsions:These findings suggest that CMC-EDBE-FA nanoparticle is expected to be safe for biomedical applications.

关键词： Carboxymethyl chitosan Folate Staphylococcus aureus Killing kinetic studies MTT assay

Antioxidative effect of folate-modified chitosan nanoparticles

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Asian Pacific Journal of Tropical Biomedicine 2011年第1期1卷 29-38页

作者： Subhankari Prasad Chakraborty Santanu Kar Mahapatra Sumanta Kumar Sahu Panchanan Pramanik somenath roy Immunology and Microbiology Laboratory Department of Human Physiology with Community HealthVidyasagar University Nanomaterials Laboratory Department of ChemistryIndian Institute of Technology

Objective:To evaluate the potency of carboxymethyl chitosan-2,2' ethylenedioxy bisethylamine-folate(CMC-EDBE-FA) on tissue injury,antioxidant status and glutathione system in tissue mitochondria and serum against nicotine-induced oxidative stress in mice.Methods: CMC-EDBE-FA was prepared on basis of carboxymethyl chitosan tagged with folic acid by covalently linkage through 2,2' ethylenedioxy bis-ethylamine.Animals were divided into four groups,i.e.,control,nicotine(1 mg/kg bw/day),CMC-EDBE-FA(1 mg/kg bw/day) and nicotine(1 mg/kg bw/day) and CMC-EDBE-FA(1 mg/kg bw/day) for 7 days.Levels of lipid peroxidation, oxidized glutathione level,antioxidant enzyme status and DNA damage were observed and compared.Results:The significantly increase of lipid peroxidation,oxidized glutathione levels and DNA damage was observed in nicotine treated group as compared with control group;those were significantly reduced in CMC-EDBE-FA supplemented group.Moreover,significantly reduced antioxidant status in nicotine treated group was effectively ameliorated by the supplementation of CMC-EDBE-FA.Only CMC-EDBE-FA treated groups showed no significant change as compared with control group;rather than it repairs the tissue damage of nicotine treated group.Conclusions:These findings suggest that CMC-EDBE-FA is non-toxic and ameliorates nicotine-induced toxicity.

关键词： Carboxymethyl chitosan Nicotine Glutathione Antioxidants Deoxyribonucleic acid fragmentation Antioxidative effect Folate Lipid peroxidation Oxidized

Nano chloroquine delivery against Plasmodium berghei NK65 induced programmed cell death in spleen

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Asian Pacific Journal of Tropical Medicine 2018年第9期11卷 540-546页

作者： Satyajit Tripathy Sourav Chattopadhyay Sandeep Kr.Dash Motlalepula G.Matsabisa somenath roy Immunology and Microbiology Laboratory Department of Human Physiology with Community HealthVidyasagar University Department of Pharmacology University of the Free State B.S.B.E.Indian Institute of Technology University of Gour Banga

Objective:To compare the protective effects of chitosan-trypolyphosphate(CS-TPP) nanoparticle conjugated chloroquine(CQ) with effect of CQ alone on the reversal of splenic damages and induction of apoptosis.Methods:Different researches have been carried out to explore the potential role of chitosan based drug delivery system against parasitic diseases.After successive Plasmodium berghei NK65 parasiste infection by intraperitoneal injection in Swiss mice and subsequent parasite development,the ROS generation,anti-apoptotic and pro apoptotic protein levels in spleen were measured.To analyze caspases,flow cytometry study was performed with annexin 桋-FITC and with PI staining.Results:The results revealed that ROS mediated caspase 3 and 9 activation and the induction of apoptosis occurred during the parasitic infection.However,CS-TPP conjugated CQ was relatively better in reversing the splenic damage compared with similar effects of CQ alone.Conclusions:This study indicates that Plasmodium berghei NK65 induces apoptosis in the spleen.The study further shows that CS-TPP nanoparticles conjugation with CQ have positive influence on the recovery of damaged host's system towards maintenance of normal homeostasis,and this is shown to be selective to CS-TPP conjugated CQ treated animals only.

关键词： Splenocyte Apoptosis Nano chloroquine Plasmodium berghei Chitosan

Nitric oxide mediated Staphylococcus aureus pathogenesis and protective role of nanoconjugated vancomycin

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Asian Pacific Journal of Tropical Biomedicine 2011年第2期1卷 102-109页

作者： Subhankari Prasad Chakraborty Santanu Kar Mahapatra Sumanta Kumar Sahu Sourav Chattopadhyay Panchanan Pramanik somenath roy Immunology and Microbiology laboratory Department of Human Physiology with Community HealthVidyasagar University Nanomaterials Laboratory Department of ChemistryIndian Institute of Technology

Objective:To investigate the relation between fruit seeds,plants residuals and appendicitis. Methods:Among cases that underwent appendectomy,the appendicitis cases having fruit seeds and undigested plant residuals in their etiology were examined retrospectively.Also, histopathological features,age,sex,and parameters of morbidity and mortality were used. Results:Fruit seed was found in one case(0.05%) with presence of pus in appendix lumen, undigested plant residuals in 7 cases(0.35%).It was determined that there were appendix inflammation in 2 of the plant residuals cases,while there were obstruction and lymphoid hyperplasia in the appendix lumen of 5 cases.No mortality was observed.Conclusions:The ratio of acute appendicitis caused by plants is minimal among all appendectomised patients, but avoidence of eating undigested fruit seeds and chewing plants well may help to prevent appendicitis.

关键词： Staphylococcus aureus Lymphocyte Nanoconjugated vancomycin Antioxidant enzymes TNF-α IL-10 Infection Anti-inflammatory Antioxidative effect Bacteremia Oxidative stress Nitric oxide Inflammatory parameter Pathogenesis