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Characterization of Myanmar Paw San Hmwe Accessions Using Functional Genetic Markers

Rice science 2015年第2期22卷 53-64页

作者： Kyaw Swar Oo Alisa KONGJAIMUN Srisawat KHANTHONG Myint YI Tin Tin MYINT siriporn korinsak Jonaliza Lanceras SIANGLIW Khin Myo MYINT Apichart VANAVICHIT Chanate MALUMPONG Theerayut TOOJINDA Rice Division Department of Agricultural Research Rice Gene Discovery Unit National Centre for Genetic Engineering and BiotechnologyNational Science and Technology Development AgencyKasetsart University Agronomy Department Faculty of Agriculture at Kamphaeng SaenKasetsart University Center of Excellence on Agricultural Biotechnology Office of Higher Education CommissionMinistry of Education Plant Breeding Faculty of Agriculture at Kamphaeng SaenKasetsart University Plant Biotechnology Center Myanmar Agriculture Service Rice Science Center Kasetsart University

Paw San Hmwe （PSM） rice has been cultivated in many areas of Myanmar for a long time. Strong aroma, good taste and its elongation during cooking are the key characteristics of PSM rice. Thirty-one PSM accessions were genotypically characterized, and their physical grain and cooking quality traits were studied. We used specific gene markers associated with aroma, apparent amylose content （AAC） and alkali spreading value to determine the alleles carried by different PSM accessions. The results revealed that six PSM accessions （PSM10, PSM12, PSM13, PSM21, PSM22 and PSM30） had a 3-bp insertion in Os2AP gene. Gel consistency （GC） allele was predominant among the PSM accessions for gelatinization temperature （GT）, however, the phenotype observed was between low and intermediate GT because of the combination of the GC allele with the presence of low GT allele at heterozygous state from the other loci of the SSIla gene. Intermediate to high AAC was observed among the PSM accessions corresponding to the haplotype identified for the single nucleotide polymorphism G/T and the （Cm）n repeat in the Wx gene. The characterization and grouping data of PSM accessions posted benefits to Myanmar seed banks, and our results will help in maintaining the integrity of PSM rice variety.

关键词： Paw San Hmwe rice aroma apparent amylose content gel consistency gelatinizationtemperature alkali spreading value functional genetic marker grain quality haplotype analysis

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Current opinion on the regulation of small intestinal magnesium absorption

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World Journal of Gastroenterology 2023年第2期29卷 332-342页

作者： siriporn Chamniansawat Nasisorn Suksridechacin Narongrit Thongon Division of Anatomy Department of Medical SciencesFaculty of Allied Health SciencesBurapha UniversityMuang 20131ChonburiThailand Biodiversity Research Centre Thailand Institute of Scientific and Technological ResearchKhlong Luang 12120Pathum ThaniThailand Division of Physiology Department of Medical SciencesFaculty of Allied Health SciencesBurapha UniversityMuang 20131ChonburiThailand

Magnesium(Mg^(2+))has an important role in numerous biological functions,and Mg^(2+)deficiency is associated with several ***,adequate intestinal absorption of Mg^(2+)is vital for *** small intestine was previously thought to absorb digested Mg^(2+)exclusively through an unregulated paracellular mechanism,which is responsible for approximately 90%of total Mg^(2+)*** studies,however,have revealed that the duodenum,jejunum,and ileum absorb Mg^(2+)through both transcellular and paracellular *** regulatory factors of small intestinal Mg^(2+)uptake also have been explored,e.g.,parathyroid hormone,fibroblast growth factor-23,apical acidity,proton pump inhibitor,and pH-sensing channel and *** mechanistic factors underlying proton pump inhibitor suppression of small intestinal Mg^(2+),such as magnesiotropic protein dysfunction,higher mucosal bicarbonate secretion,Paneth cell dysfunction,and intestinal inflammation,are currently being *** potential role of small intestinal microbiomes in Mg^(2+)absorption has also been *** this article,we reviewed the current knowledge on the mechanisms and regulatory factors of small intestinal Mg^(2+)absorption.

关键词： Hormone Magnesium absorption Paneth cells Proton pump inhibitor Regulation Small intestine

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A cation-dipole-reinforced elastic polymer electrolyte enabling long-cycling quasi-solid-state lithium metal batteries

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Journal of Energy Chemistry 2022年第12期75卷 340-348,I0009页

作者： Zhuyi Wang Yiming Wang Pan Zhai Preeyaporn Poldorn siriporn Jungsuttiwong Shuai Yuan Research Centre of Nanoscience and Nanotechnology Shanghai UniversityShanghai 200444China Center for Organic Electronic and Alternative Energy Department of Chemistry and Center of Excellence for Innovation in ChemistryFaculty of ScienceUbon Ratchathani UniversityUbon Ratchathani 34190Thailand

The application of ionic liquids(IL)in polymer electrolytes represents a safer alternative to the currently used organic solvents in lithium batteries due to their nonflammability and thermal ***,as a plasticizer,it is generally agreed that the introduction of ionic liquid usually leads to a trade-off between ion transport and mechanical properties of polymer *** we report the synthesis of an IL-embedded polymer electrolyte with both high ionic conductivity(2.77×10^(-4)S cm^(-1)at room temperature)and excellent mechanical properties(high tensile strength up to 11.4 MPa and excellent stretchability of 387%elongation at break)achieved by strong ion–dipole interactions between polymer electrolyte components,which was unveiled by the DFT ***,this polymer electrolyte also exhibits nonflammability,good thermal stability and the ability to recover reversibly from applied stress,i.e.,excellent *** highly viscoelastic polymer electrolyte enables tight interfacial contact and good adaptability with electrodes for stable lithium stripping/plating for 2000 h under a current density of 0.1 mA cm^(-2).By coupling with this polymer electrolyte,the LiFePO_(4)/Li cells exhibit outstanding cycling stability at room temperature as well as the reliability under extreme environmental temperature or being abused.

关键词： Elastic polymer electrolyte Ion-dipole interactions High tensile strength Quasi-solid-state lithium battery Long cycling

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Five years of post-validation surveillance of lymphatic filariasis in Thailand

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Infectious Diseases of Poverty 2023年第6期12卷 61-78页

作者： Prapapan Meetham Rawadee Kumlert Deyer Gopinath siriporn Yongchaitrakul Tanaporn Tootong Sunsanee Rojanapanus Chantana Padungtod Division of Vector Borne Diseases Department of Disease ControlMinistry of Public HealthNonthaburiThailand World Health Organization(WHO) Country OfceNonthaburiThailand Department of Disease Control Ministry of Public HealthNonthaburiThailand

Background The World Health Organization (WHO) validated Thailand in 2017 as having eliminated lymphatic filariasis (LF) as a public health problem with recommendations for continued surveillance. This article describes measures and progress made in Thailand with post-validation surveillance (PVS) of LF from 2018 until *** The implementation unit (IU) is a sub-village in 11 former LF endemic provinces. Human blood surveys are targeted in 10% of IUs each year. InWuchereria bancrofti areas, filaria antigen test strips (FTS) are used, and inBrugia malayi areas, antibody test kits (Filaria DIAG RAPID) are used. Positive cases are confirmed by thick blood film (TBF) and polymerase chain reaction (PCR). Vector surveys for mosquito species identification and dissection for microfilaria (Mf)/filarial larvae are done in 1% of IUs where human blood surveys are conducted. Human blood surveys using FTS are conducted among migrants in five provinces. Surveillance of cats is done in areas that previously recorded > 1.0% Mf rate among cats. Morbidity management and disability prevention (MMDP) are done every 2 years in LF-endemic areas where chronic disease patients *** From 2018 to 2022, in a total of 357 IUs in 11 provinces, human blood surveys were conducted in 145 IUs (41%) with an average population coverage of 81%. A total of 22,468 FTS and 27,741 FilariaDIAG RAPID were performed. 27 cases were detected: 3 cases ofW. bancrofti in Kanchanaburi province and 24 cases ofB. malayi in Narathiwat province. 4 cases ofW. bancrofti were detected in two provinces through routine public health surveillance. Vector surveys in 47 IUs detectedB. malayi Mf filarial larvae only in Narathiwat province. Chronic LF patients reduced from 114 in 2017 to 76 in 2022. Surveys among 7633 unregistered migrants yielded 12 cases ofW. bancrofti. Mf rate among cats in Narathiwat province declined from 1.9% in 2018 to 0.7% in 2022. MMDP assessments revealed gaps in healthcare provider’s

关键词： Lymphatic filariasis Elimination Post validation Surveillance Neglected tropical diseases Thailand

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Gasdermin D-mediated pyroptosis in myocardial ischemia and reperfusion injury:Cumulative evidence for future cardioprotective strategies

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Acta Pharmaceutica Sinica B 2023年第1期13卷 29-53页

作者： Panat Yanpiset Chayodom Maneechote Sirawit Sriwichaiin Natthaphat Siri-Angkul siriporn C.Chattipakorn Nipon Chattipakorn Cardiac Electrophysiology Research and Training Center Faculty of MedicineChiang Mai UniversityChiang Mai 50200Thailand Cardiac Electrophysiology Unit Department of PhysiologyFaculty of MedicineChiang Mai UniversityChiang Mai 50200Thailand Center of Excellence in Cardiac Electrophysiology Research Chiang Mai UniversityChiang Mai 50200Thailand Department of Oral Biology and Diagnostic Sciences Faculty of DentistryChiang Mai UniversityChiang Mai 50200Thailand

Cardiomyocyte death is one of the major mechanisms contributing to the development of myocardial infarction(MI)and myocardial ischemia/reperfusion(MI/R)*** to the limited regenerative ability of cardiomyocytes,understanding the mechanisms of cardiomyocyte death is ***,one of the regulated programmed cell death pathways,has recently been shown to play important roles in MI and MI/R *** is activated by damage-associated molecular patterns(DAMPs)that are released from damaged myocardial cells and activate the formation of an apoptosisassociated speck-like protein containing a CARD(ASC)interacting with NACHT,LRR,and PYD domains-containing protein 3(NLRP3),resulting in caspase-1 cleavage which promotes the activation of Gasdermin D(GSDMD).This pathway is known as the canonical *** has also been shown to be activated in a non-canonical pathway during MI and MI/R injury via caspase-4/5/*** of GSDMD has been shown to provide cardioprotection against MI and MI/R *** the effects of MI or MI/R injury on pyroptosis have previously been discussed,knowledge concerning the roles of GSDMD in these settings remains *** this review,the evidence from in vitro,in vivo,and clinical studies focusing on cardiac GSDMD activation during MI and MI/R injury is comprehensively summarized and *** from this review will help pave the way for a new therapeutic target in ischemic heart disease.

关键词： Pyroptosis Gasdermin D Heart Ischemia Ischemia-reperfusion injury Myocardial infarction

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Osteogenic differentiation of encapsulated cells in dexamethasone-loaded phospholipid-induced silk fibroin hydrogels

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Biomaterials Translational 2022年第3期3卷 213-220页

作者： Chavee Laomeephol Helena Ferreira Sorada Kanokpanont Jittima Amie Luckanagul Nuno M Neves siriporn Damrongsakkul Department of Pharmaceutics and Industrial Pharmacy Faculty of Pharmaceutical SciencesChulalongkorn UniversityBangkokThailand Biomaterial Engineering for Medical and Health Research Unit Chulalongkorn UniversityBangkokThailand B’s Research Group I3Bs-Research Institute on BiomaterialsBiodegradables and BiomimeticsUniversity of MinhoHeadquarters of the European Institute of Excellence on Tissue Engineering and Regenerative MedicineGuimarãesPortugal ICVS/3B’s-PT Government Associate Laboratory Braga/GuimarãesPortugal Department of Chemical Engineering Faculty of EngineeringChulalongkorn UniversityBangkokThailand Biomedical Engineering Research Center Faculty of EngineeringChulalongkorn UniversityBangkokThailand Center of Excellence in Plant-produced Pharmaceuticals Chulalongkorn UniversityBangkokThailand

The tissue engineering triad comprises the combination of cells,scaffolds and biological ***,we prepared cell-and drug-loaded hydrogels using in situ silk fibroin(SF)hydrogels induced by dimyristoyl glycerophosphoglycerol(DMPG).DMPG is reported to induce rapid hydrogel formation by SF,facilitating cell encapsulation in the hydrogel matrix while maintaining high cell viability and proliferative *** addition,DMPG can be used for liposome formulations in entrapping drug ***(Dex)was loaded into the DMPG-induced SF hydrogels together with human osteoblast-like SaOS-2 cells,then the osteogenic differentiation of the entrapped cells was evaluated in vitro and compared to cells cultured under standard *** production by cells cultured in DMPG/Dex-SF hydrogels with Dex-depleted osteogenic medium was equivalent to that of cells cultured in conventional osteogenic medium containing *** extended-release of the entrapped Dex by the hydrogels was able to provide a sufficient drug amount for osteogenic *** controlled release of Dex was also advantageous for cell viability even though its dose in the hydrogels was far higher than that in osteogenic *** results confirmed the possibility of using DMPG-induced SF hydrogels to enable dual cell and drug encapsulation to fulfil the practical applications of tissue-engineered constructs.

关键词： DMPG osteogenic differentiation silk fibroin three-dimensional cell culture tissue engineering

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Adulticidal, larvicidal, pupicidal and oviposition deterrent activities of essential oil from Zanthoxylum limonella Alston(Rutaceae) against Aedes aegypti(L.) and Culex quinquefasciatus(Say)

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Asian Pacific Journal of Tropical Biomedicine 2017年第11期7卷 967-978页

作者： Mayura Soonwera siriporn Phasomkusolsil Department of Plant Production Technology Faculty of Agricultural TechnologyKing Mongkut's Institute of Technology LadkrabangBangkok 10520Thailand Department of Entomology Armed Forces Research Institute of Medical SciencesBangkok 10400Thailand

Objective: To evaluate adulticidal, larvicidal and oviposition deterrent response of the essential oil from dried Zanthoxylum limonella(Z. limonella) fruit against Aedes aegypti(Ae. aegypti) and Culex quinquefasciatus(Cx. quinquefasciatus).Methods: Z. limonella oil was tested by biological assays at 1%, 5% and 10% concentrations in ethanol. Adulticidal efficacy was tested against the 2–3 day old adult females. Larvicidal activity was tested against immature stage of mosquitoes. Oviposition deterrence of the oil was evaluated on gravid ***: The adult mortality was observed after 24 h with the LC50 of 6.0% for ***, and 5.7% for Cx. quinquefasciatus. Larvicidal bioassay was carried out with the 10% Z. limonella oil against immature stages of Ae. aegypti and Cx. quinquefasciatus,which caused 100% mortality after 12 h and 24 h. In the larvicidal experiment,Z. limonella showed effective result at 1%, 5% and 10% concentrations with the values of LT50 Ae. aegypti = 9.78, 5.61, 0.24 h for larvae and LT50= 64.08, 21.23 h for pupae;Cx. quinquefasciatus had LT50= 28.46, 20.25, 1.01 h for larvae and LT50= 67.52, 27.96,4.11 h for pupae, respectively. Oviposition deterrence of the oil was evaluated on gravid females. In the study, 10% Z. limonella showed 100% repellency for Ae. aegypti and99.53% for Cx. quinquefasciatus. Likewise, oviposition activity indexes of these oil concentrations were all negative values ranging from–0.89 to-1.00 for Ae. aegypti and–0.64 to–0.99 for Cx. quinquefasciatus. The oviposition activity indexes values revealed that Z. limonella oil has deterrent effect, and it caused a remarkable negative response resulting in very few ***: This result indicates that Z. limonella oil can be used as an effective adulticide, larvicide and oviposition deterrent against Ae. aegypti and Cx. quinquefasciatus.

关键词： Zanthoxylum limonella Aedes aepypti Culex quinquefasciatus Adulticide Larvicide Oviposition deterrent

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Mitochondrial Diversity and Phylogeographic Patterns of Gekko gecko(Squamata: Gekkonidae) in Thailand

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Asian Herpetological Research 2019年第3期10卷 158-169页

作者： Anchalee AOWPHOL siriporn YODTHONG Attapol RUJIRAWAN KumthornTHIRAKHUPT Department of Zoology Faculty of ScienceKasetsart UniversityChatuchakBangkok 10900Thailand Department of Biology Faculty of ScienceChulalongkorn UniversityPathumwanBangkok 10330Thailand

The Tokay Gecko, Gekko gecko(Linnaeus, 1758) is widely distributed in Asia and there have been concerns regarding locally decreasing populations due to overexploitation for traditional Chinese medicine. Previous studies of the genetic relationships of G. gecko populations included few populations from Thailand. Here we investigated the phylogeographic patterns of G. gecko from different regions in Thailand using mitochondrial cytochrome b sequences. Phylogenetic analyses revealed two lineages: one(Lineage A) comprising populations from Laos, Vietnam, and Thailand;and a second(Lineage B) comprising three genetically distinct groups within Thailand alone. Some Thai populations were found to have both lineages represented within them. Highly significant genetic differentiation(FST) showed geographic population structuring in Lineage B, indicating limited gene flow among groups in Thailand. Although G. gecko has a wide distribution and is well adapted to human habitation, the observed genetic structure could potentially be explained by geographic barriers such as mountain ranges. In Lineage A, our study provided primary phylogeographic evidence for lineage mixture that might be a result of human-mediated transport. Future research should include more extensive sampling across the geographic distribution of G. gecko and a landscape genetics approach could be applied for conservation planning.

关键词： genetic structure haplotype Gekkonidae mitochondrial DNA human-mediated transport

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Changes in oviduct structure in the black tiger shrimp,Penaeus monodon,during ovarian maturation

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2012年第10期13卷 846-850页

作者： Sitthichai IAMSAARD siriporn SRIURAIRATANA Boonsirm WITHYACHUMNARNKUL Department of Anatomy Faculty of MedicineKhon Kaen UniversityKhon Kaen 40002Thailand Integrative Complementary Alternative Medicine Research and Development Group Faculty of MedicineKhon Kaen UniversityKhon Kaen 40002Thailand Centex Shrimp Faculty of ScienceMahidol UniversityBangkok 10400Thailand Department of Anatomy Faculty of ScienceMahidol UniversityBangkok 10400Thailand

Objective:To examine the structure of the oviduct of the shrimp Penaeus ***:The oviducts of *** with three different major groups of ovarian development(Group(Gr.) 1:Stages I & V;Gr.2:Stages II & III;and Gr.3:Stage IV) were examined by light,transmission electron,and scanning electron microscopies,***:The epithelium of the oviduct in Gr.1 was composed of tall simple columnar cells with their basal nuclei located on the basement membrane and its thick collagen *** Gr.2,the oviduct seemed to produce some substances and their epithelial cells became transitional with centrally located nuclei and formed some ***,the epithelial cells in Gr.3(at Stage IV) were disorganized,disrupted,and shed accumulated spherical secretory substances including some cellular contents into the ***:The structural changes of the *** oviduct were related to ovarian maturation stages(Grs.1-3).Prior to spawning,only the oviduct epithelium at ovary Stage IV produced and secreted a number of spherical secretion substances into the *** substances may act as the oviductal lubricants to facilitate the spawning process.

关键词： Oviduct Ovarian maturation Black tiger shrimp Penaeus monodon Spherical secretory substances

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Case Depth Measurement of Induction Hardening Using Image Processing

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材料热处理学报 2004年第5期25卷 564-566页

作者： Kitti Pongsathaporn Pramuk Jenkittiyon siriporn Daopiset Somnuk Watanasriyakul Production Engineering Department Engineering Faculty King Mongkut's Institute of Technology North Bangkok ThailandProduction Engineering Department Engineering Faculty King Mongkut's Institute of Technology North Bangkok ThailandProduction Engineering Department Engineering Faculty King Mongkut's Institute of Technology North Bangkok ThailandProduction Engineering Department Engineering Faculty King Mongkut's Institute of Technology North Bangkok Thailand

Case depth measurement of the induction hardened steel parts is necessary for quality control. Vickers microhardness test is the most industrially accepted method to identify the case depth. But this method is a time consuming one and it requires expensive equipment. The aim of this study is to develop a different method to determine the case depth using image processing. The surface hardened steel samples were cross cut, ground and etched with Nital. The etched macrosectioned specimens were scanned by a scanner. The scanned images were evaluated by the developed software. The principle of the software is to identify the gray level difference. The effective case depths of the surface hardened specimens obtained by Vickers microhardness test and the developed method were compared. It was found that the deviation of the developed method was ±0.12 mm at the case depth range of 0.6 - 2.0 mm and mm at the case depth range of 2.1 - 4.3 mm. The measuring time was only 20% of Vickers microhardness test. The deviation range is much lower than the tolerance case depth specification for induction hardening in general.

关键词：图像处理表面硬化深度感应淬火质量控制

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