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Residual feed intake phenotype and gender affect the expression of key genes of the lipogenesis pathway in subcutaneous adipose tissue of beef cattle

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Journal of Animal Science and Biotechnology 2019年第1期10卷 208-217页

作者： Clare mcKenna Richard K.Porter Kate A.Keogh sinead m.waters mark mcGee David A.Kenny Animal and Bioscience Research Department Teagasc Grange School of Biochemistry & Immunology Trinity College Dublin

Background: Feed accounts for up to 75% of costs in beef production systems,thus any improvement in feed efficiency(FE) will benefit the profitability of this *** feed intake(RFI) is a measure of FE that is independent of level of *** tissue(AT) is a major endocrine organ and the primary metabolic energy *** modulates a variety of processes related to FE such as lipid metabolism and glucose homeostasis and thus measures of inter-animal variation in adiposity are frequently included in the calculation of the RFI *** aim of this study was to determine the effect of phenotypic RFI status and gender on the expression of key candidate genes related to processes involved in energy metabolism within *** matter intake(DmI) and average daily gain(ADG) were measured over a period of 70 d for 52 purebred Simmental heifers(n = 24) and bulls(n = 28) with an initial BW±SD of 372±39.6 kg and 387±50.6 kg,*** feed intake was calculated and animals were ranked within gender by RFI into high(inefficient; n = 9 heifers and n = 8 bulls)and low(efficient; n = 9 heifers and n = 8 bulls) ***: Average daily gain ±SD and daily DmI ±SD for heifers and bul s were 1.2±0.4 kg and 9.1±0.5 kg,and 1.8±0.3 kg and 9.5±1 kg *** RFI heifers and bulls consumed 10% and 15% more(P measured than bulls(P mGCS2(P mGCS2 than low RFI bulls(P mals across ***: The findings of this study indicate that low RFI cattle exhibit upregulation of the molecular mechanisms governing glucose metabolism in adipose tiss

关键词： Adipose Cattle RFI SLC2A4

Helicobacter pylori:High dose amoxicillin does not improve primary or secondary eradication rates in an Irish cohort

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World Journal of Clinical Cases 2024年第16期12卷 2773-2779页

作者： Conor Costigan Aoife m O'Sullivan Jim O'Connell Shreyashee Sengupta Thomas Butler Stephen molloy Fintan John O'Hara Barbara Ryan Niall Breslin Sarah O'Donnell Anthony O'Connor sinead Smith Deirdre mcNamara Trinity Academic Gastroenterology Group School of Medicine-Trinity College DublinDublin D2Ireland Department of Gastroenterology Tallaght University HospitalDublin D24 NR0AIreland

BACKGROUND Helicobacter pylori(***)eradication rates have fallen globally,likely in large part due to increasing antibiotic resistance to traditional *** areas of high clarithromycin and metronidazole resistance such as ours,maastricht VI guidelines suggest high dose amoxicillin dual therapy(HDADT)can be considered,subject to evidence for local *** this study we assess efficacy of HDADT therapy for *** eradication in an Irish *** To assess the efficacy of HDADT therapy for *** eradication in an Irish cohort as both first line,and subsequent therapy for patients diagnosed with *** All patients testing positive for *** in a tertiary centre were treated prospectively with HDADT(amoxicillin 1 g tid and esomeprazole 40 mg bid×14 d)over a period of 8 *** was confirmed with Urea Breath Test at least 4 wk after cessation of therapy.A delta-over-baseline>4%was considered *** demographics and treatment outcomes were recorded,analysed and controlled for basic demographics and prior *** *** One hundred and ninety-eight patients were identified with *** infection,10 patients were excluded due to penicillin allergy and 38 patients refused follow up *** all 139 were included in the analysis,55%(n=76)were female,mean age was 46.6 ***,93(67%)of patients were treatment-naïve and 46(33%)had received at least one previous course of *** groups were statistically ***-reported compliance with HDADT was 97%,mild side-effects occurred in 7%.There were no serious adverse drug *** the eradication rate for our cohort was 56%(78/139).Eradication rates were worse for those with previous treatment[43%(20/46)vs 62%(58/93),P=0.0458,odds ratio=2.15].Age and Gender had no effect on eradication *** Overall eradication rates with HDADT were *** being a simple and possibly better tolerated regime,these results do not

关键词： Helicobacter pylori eradication Helicobacter pylori High dose amoxicillin High dose amoxicillin dual therapy Triple therapy

Quantitative analysis of ruminal methanogenic microbial populations in beef cattle divergent in phenotypic residual feed intake(RFI)offered contrasting diets

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Journal of Animal Science and Biotechnology 2014年第4期5卷 439-447页

作者： Ciara A Carberry David A Kenny Alan K Kelly sinead m.waters Animal and Bioscience Research Department Animal and Grassland Research and Innovation CentreTeagascGrangeDunsanyCo.MeathIreland UCD School of Agriculture Food Science and Veterinary MedicineCollege of Life SciencesUniversity College DublinBelfieldDublin 4Ireland

Background：methane（CH_4） emissions in cattle are an undesirable end product of rumen methanogenic fermentative activity as they are associated not only with negative environmental impacts but also with reduced host feed *** aim of this study was to quantify total and specific rumen microbial methanogenic populations in beef cattle divergently selected for residual feed intake（RFI） while offered（i） a low energy high forage（HF） diet followed by（ii） a high energy low forage（LF） *** fluid was collected from 14 high（H）and 14 low（L） RFI animals across both dietary *** real time PCR（qRT-PCR） analysis was conducted to quantify the abundance of total and specific rumen methanogenic *** correlation analysis was used to investigate the association between the relative abundance of methanogens and animal performance,rumen fermentation variables and diet ***：Abundance of methanogens,did not differ between RFI ***,relative abundance of total and specific methanogen species was affected（P〈 0.05） by diet type,with greater abundance observed while animals were offered the LF compared to the HF ***：These findings suggest that differences in abundance of specific rumen methanogen species may not contribute to variation in CH_4 emissions between efficient and inefficient animals,however dietary manipulation can influence the abundance of total and specific methanogen species.

关键词： Bovine qRT-PCR Residual feed intake Rumen methaongens

Timing of surgical repair of bile duct injuries after laparoscopic cholecystectomy: A systematic review

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World Journal of Hepatology 2022年第2期14卷 442-455页

作者： Patryk Kambakamba sinead Cremen Beat möckli michael Linecker Department of HPB and Transplant Surgery St.Vincent’s University Hospital DublinDublin d04 T6F4Ireland Department of Surgery Cantonal Hospital GlarusGlarus 8750Switzerland Department of Visceral and Transplantation Surgery University of Geneva HospitalsGeneva 1205Switzerland Department of Surgery and Transplantation University Medical Center Schleswig HolsteinKiel 24105Germany

BACKGROUND The surgical management of bile duct injuries(BDIs)after laparoscopic cholecystectomy(LC)is challenging and the optimal timing of surgery remains *** primary aim of this study was to systematically evaluate the evidence behind the timing of BDI repair after LC in the *** To assess timing of surgical repair of BDI and postoperative *** The mEDLINE,EmBASE,and The Cochrane Library databases were systematically screened up to August *** of bias was assessed via the Newcastle Ottawa *** primary outcomes of this review included the timing of BDI repair and postoperative *** A total of 439 abstracts were screened,and 24 studies were included with 15609 patients included in this *** the 5229 BDIs reported,4934(94%)were classified as major *** of bile duct repair was immediate(14%,n=705),early(28%,n=1367),delayed(28%,n=1367),or late(26%,n=1286).Standardization of definition for timing of repair was remarkably poor among *** for immediate repair ranged fromm24 h to>12 wk after LC)and late repair(>6 wk after LC)showed a broad *** The lack of standardization among studies precludes any conclusive recommendation on optimal timing of BDI repair after *** finding indicates an urgent need for a standardized reporting system of BDI repair.

关键词： Bile duct injury major bile duct injury Laparoscopic cholecystectomy Surgical repair Immediate repair Early repair Delayed repair Late repair Biliary reconstruction Standardization of bile duct injury repair reporting

Examination of the molecular control of ruminal epithelial function in response to dietary restriction and subsequent compensatory growth in cattle

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Journal of Animal Science and Biotechnology 2017年第1期8卷 107-118页

作者： Emma O＇Shea sinead m. waters Kate Keogh Alan K. Kelly David A. Kenny School of Agriculture and Food Science University College DublinBelfield Dublin 4Ireland Animal and Bioscience Research Department Animal & Grassland Research and Innovation Centre Teagasc Grange Dunsany Co Meath Ireland UCD Earth Institute University College Dublin Belfield Dublin 4 Ireland

Background： The objective of this study was to investigate the effect of dietary restriction and subsequent compensatory growth on the relative expression of genes involved in volatile fatty acid transport, metabolism and cell proliferation in ruminal epithelial tissue of beef cattle. Sixty Holstein Friesian bulls（mean liveweight 370 ± 35 kg;mean age 479 ± 15 d） were assigned to one of two groups：（i） restricted feed allowance（RES; n = 30） for 125 d（Period 1） followed by ad libitum access to feed for 55 d（Period 2） or（ii） ad libitum access to feed throughout（ADLIB; n = 30）. Target growth rate for RES was 0.6 kg/d during Period 1. At the end of each dietary period, 15 animals from each treatment group were slaughtered and ruminal epithelial tissue and liquid digesta harvested from the ventral sac of the rumen. Real-time q PCR was used to quantify m RNA transcripts of 26 genes associated with ruminal epithelial function. Volatile fatty acid analysis of rumen fluid from individual animals was conducted using gas ***： Diet × period interactions were evident for genes involved in ketogenesis（BDH2, P = 0.017）, pyruvate metabolism（LDHa, P = 0.048; PDHA1, P = 0.015） and cellular transport and structure（DSG1, P = 0.019; CACT, P = 0.027）. Ruminal concentrations of propionic acid（P = 0.018） and n-valeric acid（P = 0.029） were lower in RES animals, compared with ADLIB, throughout the experiment. There was also a strong tendency（P = 0.064）toward a diet × period interaction for n-butyric with higher concentrations in RES animals, compared with ADLIB, during Period ***： These data suggest that following nutrient restriction, the structural integrity of the rumen wall is compromised and there is upregulation of genes involved in the production of ketone bodies and breakdown of pyruvate for cellular energy. These results provide an insight into the potential molecular mechanisms regulating ruminal epithelial absorptive metabolism and growth following nutri

关键词： Beef cattle Compensatory growth Feed efficiency Nutrient restriction Rumen epithelium

Combined antrum and corpus biopsy protocol improves Helicobacter pylori culture success

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World Journal of Gastrointestinal Pathophysiology 2022年第1期13卷 34-40页

作者： Denise E Brennan Colm O'morain Deirdre mcNamara sinead m Smith Department of Clinical medicine Trinity College DublinTrinity CentreTallaght University HospitalDublin D24Ireland

BACKGROUND Helicobacter pylori(***)causes chronic gastritis,peptic ulcer disease,gastric adenocarcinoma and mucosa-associated lymphoid tissue *** rates have fallen,mainly due to antimicrobial *** guidelines recommend that first-line treatment is based on the local prevalence of antimicrobial resistance and that rescue therapies are guided by antimicrobial susceptibility testing(AST).However,*** culture is challenging and culture-based AST is not routinely performed in the majority of *** of *** culture from clinical specimens will enable more widespread AST to determine the most appropriate antimicrobials for *** *** To determine whether dual antrum and corpus biopsy sampling is superior to single antrum biopsy sampling for *** *** The study received ethical approval from the joint research ethics committee of Tallaght University Hospital and ***’s *** referred for upper gastrointestinal endoscopy were invited to *** were collected in tubes containing Dent’s transport medium and patient demographics were *** were used to inoculate Colombia blood agar *** were incubated under microaerobic conditions and evaluated for the presence of *** analyses were performed using Graphpad *** variables were compared using the two-tailed independent *** variables were compared using the two-tailed Fisher exact *** all cases,a P value less than 0.05 was considered *** In all,samples from 219 ***-infected patients were analysed in the *** mean age of recruited patients was 48±14.9 years and 50.7%(n=111)were *** most common endoscopic finding was gastritis(58.9%;n=129).Gastric ulcer was diagnosed in 4.6%(n=10)of patients,while duodenal ulcer was diagnosed in 2.7%(n=6).Single antrum biopsies were collected from 73 patients,whereas combined antrum

关键词： Helicobacter pylori Culture Antimicrobial susceptibility testing Antimicrobial Antrum Corpus

Dietary intake in patients with chronic pancreatitis:A systematic review and meta-analysis

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World Journal of Gastroenterology 2021年第34期27卷 5775-5792页

作者： Qurat Ul Ain Yasir Bashir Linda Kelleher David m Bourne Suzanne m Egan Jean mcmahon Laura Keaskin Oonagh m Griffin Kevin C Conlon sinead N Duggan Professorial Surgical Unit Department of SurgeryTrinity College DublinDublin 24 D24 NR0AIreland Department of Nutrition and Dietetics Tallaght University HospitalDublin 24 D24 NR0AIreland Department of Newcastle Nutrition Freeman HospitalNewcastle Upon Tyne NE77DNUnited Kingdom Library and Information Services Tallaght Tallaght University HospitalDublin 24 D24 NR0AIreland Department of Nutrition and Dietetics St.Vincent’s University HospitalDublin 4 D04 T6F4Ireland

BACKGROUND A progressive reduction in the secretion of pancreatic enzymes in patients with chronic pancreatitis(CP)results in malabsorption and ultimate ***,the pathogenesis of malnutrition is multifactorial and other factors such as chronic inflammation,alcohol excess and poor dietary intake all *** may restrict their dietary intake due to poor appetite or to avoid gastrointestinal symptoms and abdominal *** up to half of patients with chronic pancreatitis are reportedly malnourished,the dietary intake of patients with CP is relatively understudied and has not been systematically reviewed to *** To perform a systematic review and meta-analysis of the dietary intakes of patients with CP compared to healthy controls,and to compare the dietary intake of patients with alcohol-related CP and non-alcohol-related *** A systematic literature search was performed using EmBASE,mEDLINE,and Cochrane review on studies published between 1946 and August 30th,*** subjects with a diagnosis of CP who had undergone dietary assessment were included in the systematic review(qualitative analysis).Studies on patients with other pancreatic diseases or who had undergone pancreatic surgery were not *** comparing the dietary intake of patients with CP to that of healthy controls were included in the meta-analysis(quantitative analysis).metaanalysis was performed using Review manager *** Ottawa Scale(NOS)was used to assess quality of *** Of 6715 studies retrieved in the search,23 were eligible for qualitative analysis while 12 were eligible for quantitative *** the meta-analysis,the total energy(calorie)intake of patients with CP was similar to that of healthy controls[mean difference(mD):171.3;95%confidence interval(CI):-226.01,568.5;P=0.4],however patients with CP consumed significantly fewer non-alcohol calories than controls[mD:-694.1;95%CI:-1256.1,(-132.1);P=0.02].CP patients consumed m

关键词： Systematic review meta-analysis Chronic pancreatitis Dietary intake Undernutrition Alcohol-related chronic pancreatitis

Shared changes in angiogenic factors across gastrointestinal vascular conditions:A pilot study

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World Journal of Gastrointestinal Pharmacology and Therapeutics 2020年第3期11卷 40-47页

作者： Atiyekeogbebe R Douglas Grainne Holleran sinead m Smith Deirdre mcNamara TAGG Research Centre School of MedicineTrinity College DublinDublin D24Ireland

BACKGROUND Neovascularisation is common to a variety of gastrointestinal(GI)disorders with differing aetiologies and presentations;usually affecting adults above 60 *** angiogenic factors modulated by disease specific elements could be a common denominator and represent novel diagnostic and therapeutic *** yet,assessment of angiogenic factors across several GI vascular disorders associated with recurrent bleeding and anaemia has not been *** To assess serum levels of angiogenic factors in several intestinal vascular *** A case control study was performed in Tallaght University Hospital in patients with endoscopically proven small bowel angiodysplasia(SBA),portal hypertensive gastropathy(PHG),gastric antral vascular ectasia(GAVE)and nonbleeding,non-anaemic *** enzyme-linked immunosorbent assay,concentrations of Angiopoietin 1(Ang-1),Ang-2 and vascular endothelial growth factor(VEGF)were measured from 2 serum tubes of blood following informed *** relative expression of Ang-1 and Ang-2 and Ang-1/2 ratio was calculated and compared between *** analysis was applied using a t-test,and a P value ofmples were tested:10 SBA,11 PHG,8 GAVE and 15 *** age 60(range 20-85)years and 20(45%)were *** were significantly younger(49 years vs 66 years,P=0.0005).There was no difference in VEGF levels between the groups(P=0.6).SBA,PHG and GAVE Ang-1 levels were similar and were significantly lower than controls,(P=0.0002,95%CI:241 to 701).Ang-2 levels were statistically higher in PHG and GAVE groups compared to controls(P= 0.01, 95%CI: 77.8 to 668) and as a result, also had a lower Ang-1/2 ratioscompared to controls. While SBA Ang-2 levels were higher than controls, this didnot reach statistical significance. Neither age nor haemoglobin level, which wassimilar between disease groups, could explain the difference. In addition, themedian Ang-1/A

关键词： Gastric antral vascular ectasia Portal hypertensive gastropathy Angiodysplasia Angiopoietins Angiogenic factors Recurrent bleeding

Nurses' views of fundamental relational skills used in clinical practice: a cross-sectional pilot study

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Frontiers of Nursing 2021年第3期8卷 223-232页

作者： Fiona Timmins Jan m.A.de Vries Yvonne muldowney Catherine mc Cabe Jacqueline Whelan sinead Buckley Sandra mcCarthy The School of Nursing and midwifery Trinity CollegeDublin 2Ireland Portlaoise General Hospital BallyroanPortlaoiseCo.LaoisIreland

Objective:Effective communication skills are one of the core competencies of nursing curricula *** are generally regarded as *** our complete trust in the profession,deficiencies and gaps ***,it is not clear to which extent nurses use key communication skills in practice,and whether or not confident in using these skills compounds environmental issues that *** study explored nurse’s confidence and application of relational skills competencies in nursing ***:A 13-item online survey was used to collect ***:Being self-aware on key areas where there was more *** also lacked confidence in exploring the impact of their personal feelings and values on their *** were also less confident on responding appropriately to instances of unsafe or unprofessional practice and using information and communication systems and ***:Given the potential impact of poor relational skills on quality client care,an increased emphasis on caring and compassion,and the ever-expanding use of communication technologies,there is a need to explore the need for reflective practice to enhance continuous professional development for nurses to enhance their relational skills.

关键词： communication relational nurse caring nursing competencies technology listening