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Ultrastructural and enzymatic alterations in the ovary of Rhodnius prolixus infected with Trypanosoma rangeli

Asian pacific Journal of Tropical Medicine 2022年第2期15卷 71-78页

作者： Guilherme S.Machado Rosane L.Lopes simone S.c.Oliveira simone p.c.freitas Jacenir R.Santos-Mallet AndréL.S.Santos Denise Feder Suzete A.O.Gomes programa de pós-Graduação em ciências e Biotecnologia Instituto de BiologiaUniversidade Federal FluminenseNiteróiRJBrazil Laboratório de Biodiversidade de Insetos e patógenos Departamento de Biologia GeralInstituto de BiologiaUniversidade Federal FluminenseNiteróiRJBrazil Laboratório de Estudos Avançados de Microrganismos Emergentes e Resistentes Departamento de Microbiologia GeralInstituto de Microbiologia Paulo de GóesUniversidade Federal do Rio de JaneiroRJBrazil Fundação Oswaldo cruz piauí TeresinaPIBrazil Laboratório Interdisciplinar de Vigilância Entomológica em Díptera e Hemíptera Instituto Oswaldo CruzFundação Oswaldo CruzRio de JaneiroRJBrazil Universidade Iguaçu Nova IguaçuRJBrazil Laboratório de Biologia de Insetos Departamento de Biologia GeralInstituto de BiologiaUniversidade Federal FluminenseNiteróiRJBrazil

Objective:To investigate the morphological structure of ovarian follicular cells and biochemical parameters of both ovaries and fat bodies(sites of vitellogenesis)from Rhodnius(R.)prolixus infected with Trypanosoma(T.)***:Adult virgin females of *** were fed upon a membrane apparatus containing heat-inactivated citrated rabbit blood and a suspension of *** epimastigotes(Macias strain).Females from the control group and all the males received parasite-free *** electron microscopy was used to reveal the morphological aspects of ovarian follicle cells in both control and parasite-infected *** profile,proteolytic activities and Western blotting analyses were performed in either ovary or fat body samples of control and parasite-infected ***:According to the ultrastructural data,*** infection elicited a degeneration process in the ovarian follicular cells of *** assays indicated a reduction in the activity of aspartic peptidases in the ovary and fat body from parasite-infected group,while a significant increase in the cysteine peptidase activity was measured in both insect ***,immunoblotting revealed that vitellogenin was overexpressed in the ovary of parasite-infected ***:*** infection seems to elicit an early programmed cell death in the ovarian follicle cells as well as induces the modulation on the activities of different peptidase classes in either ovaries or fat bodies and the overexpression of the vitellogenin in the ovary of ***.

关键词： Trypanosoma rangeli Rhodnius prolixus Ovarian follicle Vitellogenin Aspartic protease cysteine protease

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The initial stages of Li_(2)O_(2) formation during oxygen reduction reaction in Li-O_(2) batteries:The significance of Li_(2)O_(2) in charge-transfer reactions within devices

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Journal of Energy chemistry 2024年第1期88卷 223-231,I0006页

作者： Daniela M.Josepetti Bianca p.Sousa simone A.J.Rodrigues Renato G.freitas Gustavo Doubek Advanced Energy Storage Division LABCenter for Innovation on New EnergiesSchool of Chemical EngineeringUniversity of CampinasAv Albert Einstein 50013083-852CampinasSPBrazil Institute of physics&Department of chemistry Laboratory of Computational MaterialsFederal University of Mato Grosso78060-900CuiabáMTBrazil

Lithium-oxygen batteries are a promising technology because they can greatly surpass the energy density of lithium-ion ***,this theoretical characteristic has not yet been converted into a real device with high *** with air contamination,metallic lithium reactivity,and complex discharge and charge reactions are the main issues for this technology.A fast and reversible oxygen reduction reaction(ORR)is crucial for good performance of secondary batteries',but the partial knowledge of its mechanisms,especially when devices are concerned,hinders further *** this perspective,the present work uses operando Raman experiments and electrochemical impedance spectroscopy(EIS)to assess the first stages of the discharge processes in porous carbon electrodes,following their changes cycle by cycle at initial operation.A growth kinetic formation of the discharge product signal(Li_(2)O_(2))was observed with operando Raman,indicating a first-order reaction and enabling an analysis by a microkinetic *** solution mechanism in the evaluated system was ascribed for an equivalent circuit with three time *** the time constant for the anode interface reveals to remain relatively constant after the first discharge,its surface seemed to be more *** model indicated that the reaction occurs at the Li_(2)O_(2) surface,decreasing the associated resistance during the initial discharge ***,the growth of Li_(2)O_(2) forms a hetero-phase between Li_(2)O_(2)/electrolyte,while creating a more compact and homogeneous on the Li_(2)O_(2)/cathode *** methodology here described thus offers a way of directly probing changes in surface chemistry evolution during cycling from a device through EIS analysis.

关键词： Li-O_(2)battery Operando Raman analysis Equivalent circuit modeling Time-constant distribution

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cavity-enhanced photoacoustic dual-comb spectroscopy

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Light(Science & Applications) 2024年第4期13卷 610-618页

作者： Zhen Wang Qinxue Nie Haojia Sun Qiang Wang simone Borri paolo De Natale Wei Ren Department of Mechanical and Automation Engineering The Chinese University of Hong KongNew TerritoriesHong Kong SARChina. State Key Laboratory of Applied Optics Changchun Institute of OpticsFine Mechanics and PhysicsChinese Academy of Sciences130033 ChangchunChina. cNRINO-Istituto Nazionale di Ottica and LENS-European Laboratory for Nonlinear Spectroscopy50019 Sesto FiorentinoItaly

photoacoustic dual-comb spectroscopy(DcS),converting spectral information in the optical frequency domain to the audio frequency domain via multi-heterodyne beating,enables background-free spectral measurements with high resolution and broad ***,the detection sensitivity remains limited due to the low power of individual comb lines and the lack of broadband acoustic ***,we develop cavity-enhanced photoacoustic DcS,which overcomes these limitations by using a high-finesse optical cavity for the power amplification of dual-frequency combs and a broadband acoustic resonator with a flat-top frequency *** demonstrate high-resolution spectroscopic measurements of trace amounts of c2H2,NH3 and cO in the entire telecommunications *** method shows a minimum detection limit of 0.6 ppb c2H2 at the measurement time of 100 s,corresponding to the noise equivalent absorption coefficient of 7×10−10 cm−*** proposed cavityenhanced photoacoustic DcS may open new avenues for ultrasensitive,high-resolution,and multi-species gas detection with widespread applications.

关键词： individual cavity spectroscopy

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ZrB_(2)-Sic ceramics toughened with oriented paper-derived graphite for a sustainable approach

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Journal of Advanced ceramics 2024年第2期13卷 207-219页

作者： Luca Zoli Francesca Servadei simone Failla Matteo Mor Antonio Vinci pietro Galizia Diletta Sciti cNR-ISSMc Institute of ScienceTechnology and Sustainability for CeramicsFaenza I-48018Italy

carbon fibre-reinforced ultra-high temperature ceramics(UHTcs)are considered a class of promising materials for several applications,the most appealing ones being in the aerospace *** is necessary to overcome the brittleness and low thermal shock resistance of ceramics and is often provided through the addition of carbon fibres or other carbon-based phases,such as nanotubes,graphene,and *** present work is focused on the toughening of UHTcs through incorporation of 30-50 vol% thin,ordered graphite layers from conventional filter paper followed by hot pressing *** filter paper was selected because it undergoes thermolysis with no melting stage forming a strong carbonaceous residue that can be used as *** and mechanical properties of toughened composites were compared to those of other materials reinforced with short carbon fibres and the effect of different distribution of graphite was *** of graphite allowed toughness to be increased from 3−4 Mpa·m^(1/2)(for unreinforced materials)to 5.1−5.5 Mpa·m^(1/2),similar to results obtained with short fibre *** high-temperature properties,such as strength and toughness as well as oxidation resistance at 1500℃,were also examined.

关键词： borides paper-derived graphite microstructure toughness sustainability

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Effect of resistance training volume on body adiposity,metabolic risk,and inflammation in postmenopausal and older females:Systematic review and meta-analysis of randomized controlled trials

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Journal of Sport and Health Science 2024年第2期13卷 145-159页

作者： paulo Ricardo prado Nunes pamela castro-e-Souza Anselmo Alves de Oliveira Bruno de freitas camilo Gislaine cristina-Souza Lucio Marques Vieira-Souza Marcelo Augusto da Silva carneiro Department of Body and Human Movement Minas Gerais State University(UEMG)37902-092 PassosMGBrazil University center of planalto de Araxa(UNIARAXA) 38180-129 AraxaMGBrazil physical Activity Health and Ageing Research Group(GPASE)Minas Gerais State University(UEMG)37902-092 PassosMGBrazil Exercise and Nutrition Research Group(GpEN) Minas Gerais State University(UEMG)37902-092 PassosMGBrazil Sport Sciences and physical Exercise Study and Research Group(GEpcEEX) Minas Gerais State University(UEMG)37902-092 PassosMGBrazil Metabolism Nutrition and Exercise LaboratoryPhysical Education and Sport CenterLondrina State University(UEL)86050-070 LondrinaPRBrazil postgraduate program in physical Education Federal University of Sergipe(UFS)49107-230 Sao CristovaoSEBrazil

purpose:This meta-analytical study aimed to explore the effects of resistance training(RT) volume on body adiposity,metabolic risk,and inflammation in postmenopausal and older ***:A systematic search was performed for randomized controlled trials in pubMed,Scopus,Web of Science,and *** controlled trials with postmenopausal and older females that compared RT effects on body adiposity,metabolic risk,and inflammation with a control group(cG) were *** reviewers selected the studies,extracted the data,and performed the risk of bias and certainty of the evidence(Grading of Recommendations,Assessment,Development,and Evaluation(GRADE)) *** body and abdominal adiposity,blood lipids,glucose,and c-reactive protein were included for meta-analysis.A random-effects model,standardized mean difference(Hedges’ g),and 95% confidence interval(95%cI) were used for ***:Twenty randomized controlled trials(overall risk of bias:some concerns;GRADE:low to very low) with overweight/obese postmenopausal and older females were *** groups were divided into low-volume RT(LVRT,~44 sets/week) and high-volume RT(HVRT,~77 sets/week).Both RT groups presented improved body adiposity,metabolic risk,and inflammation when compared to ***,HVRT demonstrated higher effect sizes than LVRT for glucose(HVRT=-1.19;95%cI:-1.63 to-0.74;LVRT=-0.78;95%cI:-1.15 to-0.41) and c-reactive protein(HVRT=-1.00;95%cI:-1.32 to-0.67;LVRT=-0.34;95%cI,-0.63 to-0.04)) when compared to ***:compared to cG,HVRT protocols elicit greater improvements in metabolic risk and inflammation outcomes than LVRT in overweight/obese postmenopausal and older females.

关键词： c-reactive protein Fat mass Lipid profile Menopause Strength training

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Effects of early postnatal gastric and colonic microbiota transplantation on piglet gut health

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Journal of Animal Science and Biotechnology 2024年第2期15卷 645-658页

作者： christina Larsen simone Margaard Offersen Anders Brunse Mattia pirolo Soumya Kanti Kar Luca Guadabassi Thomas Thymann Department of Veterinary and Animal Science University of CopenhagenDyrlagevej 681870 Frederiksberg CDenmark Animal Nutrition Wageningen Livestock ResearchWageningen University&Research1 De Elst6708 WageningenThe Netherlands

Background Diarrhea is a major cause of reduced growth and mortality in piglets during the suckling and weaning periods and poses a major threat to the global pig *** and gut dysbiosis may in part be prevented via improved early postnatal microbial colonization of the *** secure better postnatal gut colonization,we hypothesized that transplantation of colonic or gastric content from healthy donors to newborn recipients would prevent diarrhea in the recipients in the post-weaning *** objective was to examine the impact of transplanting colonic or gastric content on health and growth parameters and paraclinical parameters in recipient single-housed piglets exposed to a weaning transition and challenged with enterotoxigenic Escherichia coli(ETEc).Methods Seventy-two 1-day-old piglets were randomized to four groups:colonic microbiota transplantation(cMT,n=18),colonic content filtrate transplantation(ccFT,n=18),gastric microbiota transplantation(GMT,n=18),or saline(cON,n=18).Inoculations were given on d 2 and 3 of life,and all piglets were milk-fed until weaning(d 20)and shortly after challenged with ETEc(d 24).We assessed growth,diarrhea prevalence,ETEc concentration,organ weight,blood parameters,small intestinal morphology and histology,gut mucosal function,and microbiota composition and *** Episodes of diarrhea were seen in all groups during both the milk-and the solid-feeding phase,possibly due to stress associated with single ***,ccFT showed lower diarrhea prevalence on d 27,28,and 29 compared to cON(all pccFT also showed a lower ETEc prevalence on d 27(pcMT showed a higher alpha diversity and a difference in beta diversity compared to cON(pparaclinical endpoints were similar across *** In conclusion,only ccFT reduced ETEc-related post-weaning ***,the protective effect was marginal,suggesting that higher doses,more effective modalities of administration

关键词： colonic content filtrate transplantation colonic microbiota transplantation Gastric microbiota transplantation Gut microbiota Mucosa Neonatal post-weaning diarrhea

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Lower limb balance,ankle dorsiflexion,orofacial tissue pressure,and occlusal force of rugby players

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Sports Medicine and Health Science 2024年第2期6卷 173-178页

作者： Rafael R.Machado Marcelo palinkas paulo B.de Vasconcelos Sara Gollino Veridiana W.Arnoni Marcos Vinicios R.prandi Isabela H.Regalo Selma Siessere simone c.H.Regalo School of Dentistry of Ribeirao preto University of Sao PauloBrazil National Institute for Translational Medicine(INcT-TM) Ribeirao PretoBrazil

This cross-sectional study examined the lower limb balance,ankle dorsiflexion,orofacial tissue pressure,and occlusal strength of rugby ***-six participants were divided into groups:rugby players(n=13)and healthy sedentary adults(n=13).participants underwent an analysis of lower limb balance using a composite score(Y-Balance Test).Ankle dorsiflexion was measured using the Lunge *** Iowa Oral performance Instrument was employed to measure orofacial tissue *** force was measured with a dynamometer,and T-Scan assessed occlusal contact *** were analyzed using the t-test(pcOVA with age and weight as covariates,where it is possible to verify that these factors did not influence the results *** differences were observed in the balance of the right(p=0.07)and left(p=0.02)lower limbs,where rugby players had lower composite *** were significant differences in the right(p=0.005)and left(p=0.004)lunges,with rugby players showing lower values,as well as lower tongue pressure(p=0.01)and higher lip pressure(p=0.03),with significant differences to sedentary *** was no significant difference in molar bite force and distribution occlusal contacts between *** seems to reduce lower limb displacement,cause ankle hypomobility,lead to changes in orofacial tissues,particularly the tongue and *** study is significant for identifying significant differences between rugby players and sedentary individuals,providing new insights into the impact of rugby on health and performance,which can benefit sports training and injury prevention.

关键词： Rugby Y-balance test Lunge test Orofacial tissues Occlusal force

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corrigendum to ‘A novel STK11 gene mutation (c.388dupG,***130Glyfs∗33) in a peutz-Jeghers family and evidence of higher gastric cancer susceptibility associated with alterations in STK11 region aa 107–170’ [Gene Dis (9) (2022) 288–291]

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Genes & Diseases 2024年第2期11卷 1082-1083页

作者： Giovanna Forte Filomena cariola Katia De Marco Andrea Manghisi Filomena Anna Guglielmi Raffaele Armentano Giuseppe Lippolis pietro Giorgio cristiano simone Vittoria Disciglio Medical Genetics National Institute of Gastroenterology “S. de Bellis” Research Hospital Castellana Grotte Bari 70013 Italy Histopathology Unit National Institute of Gastroenterology “S. de Bellis” Research Hospital Castellana Grotte Bari 70013 Italy Department of General Surgery National Institute of Gastroenterology “S. de Bellis” Research Hospital Castellana Grotte Bari 70013 Italy Gastroenterology and Digestive Endoscopy Unit National Institute of Gastroenterology “S. de Bellis” Research Hospital Castellana Grotte Bari 70013 Italy Department of Biomedical Sciences and Human Oncology (DIMO) Medical Genetics University of Bari Aldo Moro Piazza Umberto I Bari 70124 Italy

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clinical-based phenotypes in children with pediatric post-cOVID-19 condition

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World Journal of pediatrics 2024年第7期20卷 682-691页

作者： Lieke c.E.Noij Jelle M.Blankestijn coen R.Lap Marlies A.van Houten Giske Biesbroek Anke-Hilse Maitland-van der Zee Mahmoud I.Abdel-Aziz Johannes B.van Goudoever Mattijs W.Alsem caroline L.H.Brackel Kim J.Oostrom simone Hashimoto Suzanne W.J.Terheggen-Lagro Department of pediatric pulmonology and Allergy Amsterdam UMCLocation AMCEmma Children’s HospitalAmsterdam University Medical CenterRoom G2-222Meibergdreef 91105AZAmsterdamThe Netherlands Department of pulmonary Medicine Amsterdam UMCAmsterdamThe Netherlands Department of pediatrics Spaarne GasthuisHoofddorpThe Netherlands Department of pediatrics Emma Children’s HospitalAmsterdam University Medical CenterAmsterdamThe Netherlands Department of Rehabilitation Medicine Amsterdam Movement SciencesMeibergdreef 9AmsterdamThe Netherlands Department of paediatric pulmonology Department of PaediatricsTergooi MCHilversumThe Netherlands Department of child and Adolescent psychiatry and psychosocial care Amsterdam UMCAmsterdam Reproduction and DevelopmentAmsterdamThe Netherlands

Background pediatric post coronavirus disease 2019(cOVID-19)condition(ppcc)is a heterogeneous syndrome,which can significantly affect the daily lives of *** study aimed to identify clinically meaningful phenotypes in children with ppcc,to better characterize and treat this *** participants were children with physician-diagnosed ppcc,referred to the academic hospital Amsterdam UMc in the Netherlands between November 2021 and March *** factors and information on post-cOVID symptoms,comorbidities,and impact on daily life were *** clusters were identified using an unsupervised and unbiased approach for mixed data *** Analysis of 111 patients(aged 3–18 years)revealed three distinct clusters within *** 1(n=62,median age=15 years)predominantly consisted of girls(74.2%).These patients suffered relatively more from exercise intolerance,dyspnea,and smell *** 2(n=33,median age=13 years)contained patients with an even gender distribution(51.5%girls).They suffered from relatively more sleep problems,memory loss,gastrointestinal symptoms,and *** 3(n=16,median age=11 years)had a higher proportion of boys(75.0%),suffered relatively more from fever,had significantly fewer symptoms(median of 5 symptoms compared to 8 and 10 for clusters 1 and 2 respectively),and experienced a lower impact on daily *** This study identified three distinct clinical ppcc phenotypes,with variations in sex,age,symptom patterns,and impact on daily *** findings highlight the need for further research to understand the potentially diverse underlying mechanisms contributing to post-cOVID symptoms in children.

关键词： Adolescents clusters Long cOVID pediatric phenotypes post-cOVID syndrome

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proficiency score as a predictor of early trifecta achievement during the learning curve of robot-assisted radical prostatectomy for high-risk prostate cancer:Results of a multicentric series

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current Urology 2024年第2期18卷 110-114页

作者： Umberto Anceschi Rocco simone Flammia Antonio Tufano Michele Morelli Antonio Galfano Lorenzo Giuseppe Luciani Leonardo Misuraca paolo Dell’Oglio Gabriele Tuderti Aldo Brassetti Maria consiglia Ferriero Alfredo Maria Bove Riccardo Mastroianni Francesco prata Isabella Sperduti Giovanni petralia SilviaSecco Ettore Di Trapani DanieleMattevi Tommaso cai Aldo Massimo Bocciardi Giuseppe simone Department of Urology IRCCS“Regina Elena”National Cancer InstituteRomeItaly Urologic clinic Department of Maternal-Child and Urologic SciencesSapienza University of RomeItaly Ospedale Niguarda ca’Granda Department of UrologyMilanItaly Department of Urology APSS Santa Chiara Regional HospitalTrentoItaly Department of Biostatistical Unit IRCCS Regina Elena National Cancer InstituteRomeItaly

Background:Recently,an innovative tool called“proficiency score”was introduced to assess the learning curve for robot-assisted radical prostatectomy(RARp).However,the initial study only focused on patients with low-risk prostate cancer forwhompelvic lymph node dissection(pLND)was not *** address this issue,we aimed to validate proficiency scores of a contemporarymulticenter cohort of patients with high-risk prostate cancer treated with RARp plus extended pLND by trainee *** andmethods:Between 2010 and 2020,4 Italian institutional prostate-cancer datasets weremerged and queried for“RARp”and“high-risk prostate cancer.”High-risk prostate cancer was defined according to the most recent European Association of Urology guidelines as follows:prostate-specific antigen>20 ng/mL,International Society ofUrological pathology≥4,and/or clinical stage(cT)≥2c on preoperative *** selected cohort(n=144)included clinical cases performed by trainee surgeons(n=4)after completing their RARp learning curve(50 procedures for low-risk prostate cancer).The outcome of interest,the proficiency score,was defined as the coexistence of all the following criteria:a comparable operation time to the interquartile range of the mentor surgeon at each center,absence of any significant perioperative complications clavien-Dindo Grade 3–5,no perioperative blood transfusions,and negative surgical margins.A logistic binary regression model was built to identify the predictors of 1-year trifecta achievement in the trainee *** all statistical analyses,a 2-sided pconsidered ***:A proficiency score was achieved in 42.3%*** univariable level,proficiency score was associated with 1-year trifecta achievement(odds ratio,8.77;95%confidence interval,2.42–31.7;p=0.001).After multivariable adjustments for age,nerve-sparing,and surgical technique,the proficiency score independently predicted 1-year trifecta achievement(odds ratio,9.58;95%confidence i

关键词： Trifecta Learning curve Robot-assisted radical prostatectomy High-risk prostate cancer

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