Effects of early postnatal gastric and colonic microbiota transplantation on piglet gut health

作     者：Christina Larsen Simone Margaard Offersen Anders Brunse Mattia Pirolo Soumya Kanti Kar Luca Guadabassi Thomas Thymann Christina Larsen;Simone Margaard Offersen;Anders Brunse;Mattia Pirolo;Soumya Kanti Kar;Luca Guadabassi;Thomas Thymann

作者机构：Department of Veterinary and Animal ScienceUniversity of CopenhagenDyrlagevej 681870 Frederiksberg CDenmark Animal NutritionWageningen Livestock ResearchWageningen University&Research1 De Elst6708 WageningenThe Netherlands 

出 版 物：《Journal of Animal Science and Biotechnology》 (畜牧与生物技术杂志（英文版）)

年 卷 期：2024年第15卷第2期

页      面：645-658页

核心收录：

学科分类：0905[农学-畜牧学] 09[农学] 

基　　金：support by European Union's Horizon 2020 Research and Innovation Program under Grant Agreement No.862829 project AVANT-Alternatives to Veterinary ANTimicrobials 

主　　题：Colonic content filtrate transplantation Colonic microbiota transplantation Gastric microbiota transplantation Gut microbiota Mucosa Neonatal Post-weaning diarrhea 

摘      要：Background Diarrhea is a major cause of reduced growth and mortality in piglets during the suckling and weaning periods and poses a major threat to the global pig *** and gut dysbiosis may in part be prevented via improved early postnatal microbial colonization of the *** secure better postnatal gut colonization,we hypothesized that transplantation of colonic or gastric content from healthy donors to newborn recipients would prevent diarrhea in the recipients in the post-weaning *** objective was to examine the impact of transplanting colonic or gastric content on health and growth parameters and paraclinical parameters in recipient single-housed piglets exposed to a weaning transition and challenged with enterotoxigenic Escherichia coli(ETEC).Methods Seventy-two 1-day-old piglets were randomized to four groups:colonic microbiota transplantation(CMT,n=18),colonic content filtrate transplantation(CcFT,n=18),gastric microbiota transplantation(GMT,n=18),or saline(CON,n=18).Inoculations were given on d 2 and 3 of life,and all piglets were milk-fed until weaning(d 20)and shortly after challenged with ETEC(d 24).We assessed growth,diarrhea prevalence,ETEC concentration,organ weight,blood parameters,small intestinal morphology and histology,gut mucosal function,and microbiota composition and *** Episodes of diarrhea were seen in all groups during both the milk-and the solid-feeding phase,possibly due to stress associated with single ***,CcFT showed lower diarrhea prevalence on d 27,28,and 29 compared to CON(all P0.05).CcFT also showed a lower ETEC prevalence on d 27(P0.05).CMT showed a higher alpha diversity and a difference in beta diversity compared to CON(P0.05).Growth and other paraclinical endpoints were similar across *** In conclusion,only CcFT reduced ETEC-related post-weaning ***,the protective effect was marginal,suggesting that higher doses,more effective modalities of administration