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Essential Oils from Mentha piperita,Cymbopogon citratus,Rosmarinus officinalis,Peumus boldus and Foeniculum vulgare:Inhibition of Phospholipase A_(2) and Cytotoxicity to Human Erythrocytes

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American Journal of Plant Sciences 2017年第9期8卷 2196-2207页

作者： Danubia aparecida de Carvalho Selvati Rezende Maria das Gracas Cardoso Rafaela Vieira Souza Maria Luisa teixeira Rafaela Magalhaes Brandao Vanuzia Rodrigues Fernandes Ferreira Jéssica Oliveira e Nogueira Maisa Lamounier Magalhaes Silvana Marcussi David Lee Nelson Departamento de Química Universidade Federal de Lavras Lavras Brasil Departamento de Ciência dos Alimentos Universidade Federal de Lavras Lavras Brasil Universidade Federal do Vale do Jequitinhonha e Mucuri Diamantina Brasil

The essential oils from Mentha piperita, Cymbopogon citratus, Rosmarinus officinalis, Peumus boldus and Foeniculum vulgare were extracted by hydrodistillation and characterized and quantified by GC-MS and GC-DIC. The oils induced hemolysis with all the doses evaluated (0.6 to 1.8 μL), and the diameters of the halos varied between 9 and 15 mm. Pre-incubation of P. boldus oil with Bothrops jararacussu venom resulted in potentiation of venom-induced hemolysis (30%) (proteases and phospholipases A2). The essential oil from M. piperita (0.6 μL) inhibited venom-induced hemolysis by 45%, whereas 0.6 μL of R. officinalis oil increased the hemolysis by 20%. For the essential oil from F. vulgare, 100% inhibition of activity (0.6 and 1.2 μL) was observed. The application of C. citratus oil induced hemolysis with all the volumes evaluated. Phospholipase activity induced by the venom was only inhibited (10%) with the 0.6 μL volume of R. officinalis oil. The oils from M. piperita and F. vulgare (1.8 μL) and C. citratus oil (0.6 μL) potentiated the phospholipase activity. The results highlight the need for a broad characterization and regulation of the use of natural products, because they can have therapeutic or toxic actions.

关键词： Natural Products Snake Venoms Hemolysis Pharmacological Potential

“Cachacas”(Sugarcane Spirit)Aged Quantitation of Phenolic Compounds,Antibacterial and Antioxidant Activity

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American Journal of Plant Sciences 2014年第20期5卷 2935-3942页

作者： Leonardo Milani Avelar Rodrigues Maria das Gracas Cardoso Luís Roberto Batista Wilder Douglas Santiago Juliana Maria Valério Resende Juliana de Andrade Santiago Marcos de Souza Gomes Milene aparecida Andrade Maria Luisa teixeira Fabiana Reinis Franca Passamani Department of Food Science Federal University of LavrasLavrasBrazil Department of Chemistry Federal University of LavrasLavrasBrazil

The “cachaça” is currently one of the fastest growing agro-industrial industries in the country as the drink considered as a symbol of Brazilian nationality. This study aimed to quantify the phenolic compounds, evaluate the antioxidant and antibacterial activity of different samples of aged “cachaça”. The physic-chemical analysis was performed at the Brandy Quality Laboratory of the Federal University of Lavras, and the determination of phenolic compounds was performed by High Performance Liquid Chromatography (HPLC). The antimicrobial activity evaluation was held at the Food Mycology Laboratory through the cavity agar diffusion technique and by steam using the micro-organisms Escherichia coli, Staphylococcus aureus, Salmonella choleraesuis, Listeria monocytogenes and Pseudomonas aeruginosa. The antioxidant activity was evaluated using the DPPH radical sequestering method. The values obtained for the phenolic compounds ranged from 0.41 to 9.69 mg/L, observing the predominance of syringaldehyde, gallic acid and vanillic acid. The phenolic extracts showed a satisfactory antibacterial activity for both Gram-negative and Gram-positive but did not show inhibition against the bacteria Pseudomonas aeruginosa. The antioxidant activity was evidenced by the DPPH test for cachaças tested and the “cachaça” aged in oak barrel showed the highest activity, followed by “cachaça” aged in jequitibá, amburana and balm barrels.

关键词： Syringaldehyde Gallic Acid Vanillic Acid Bacteria DPPH Test

Essential Oils from <i>Lippia origanoides</i>Kunth. and <i>Mentha spicata</i>L.: Chemical Composition, Insecticidal and Antioxidant Activities

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American Journal of Plant Sciences 2014年第9期5卷 1181-1190页

作者： Maria Luisa teixeira Maria das G. Cardoso Ana Cristina S. Figueiredo Jair C. Moraes Franscinely A. Assis Juliana de Andrade David L. Nelson Marcos de Souza Gomes Josefina aparecida de Souza Luiz Roberto Marques de Albuquerque Department of Chemistry Federal University of Lavras Lavras Brazil Department of Entomology Federal University of Lavras Lavras Brazil Federal University of Vales de Jequitinhonha e Mucuri Diamantina Brazil Universidade de Lisboa Faculdade de Ciências de Lisboa Centro de Biotecnologia Vegetal Lisboa Portugal

This work describes the chemical composition of the essential oils extracted from fresh leaves of Lippia origanoides Kunth. and Mentha spicata L. and their antioxidant and insecticidal activities. The essential oils were extracted by steam distillation using a modified Clevenger apparatus and subsequently analyzed by gas chromatography-flame ionization detector and gas chromatography-mass spectrometry. The antioxidant activities were determined by the β-carotene-linoleic acid method and by sequestration of the 2,2-diphenyl-1-picryl-hidrazila radical. The concentrations of the essential oils and the synthetic standard, butylated hydroxyltoluene, were 25, 50, 100, 150, 200, 250, 300 and 500 μg mL-1. Insecticidal activity was analyzed by non-preference with choice and no choice against the aphid Myzus persicae Sulzer. Gas chromatography analysis of the essential oil from Lippia origanoides Kunth. revealed carvacrol (41.51%), p-cymene (18.36%), γ-terpinene (17.03%) and thymol (4.86%) as major constituents, and the essential oil from Mentha spicata L. contained piperitona (81.18%), piperitenone (14.57%) and limonene (1.47%) as the principal components. The essential oils and the standard exhibited dose-dependent antioxidant activities at the concentrations tested. The essential oils were shown to be potential agents in the integrated management of the aphid Myzus persicae Sulzer.

关键词： Antioxidant Activity Insecticidal Activity Lamiaceae Natural Products Verbenaceae

Protective Properties in Hymenaea martiana Hayne against Multi-drug-resistant Staphylococcus aureus

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Veterinary Science Research 2020年第2期2卷 16-23页

作者： Dielson da Silva Vieira Tânia Maria Sarmento da Silva Timothy AHackett Marianade Barros Weslen Fabrício Pires teixeira Juliana Campos Pereira Diniz Pedro HenriqueGorni Sanely Lourenço da Costa Maria aparecida Scatamburlo Moreira MateusMatiuzzi da Costa Francisco Leydson Formiga Feitosa São Paulo State University“Júlio de Mesquita Filho”(UNESP) School of Veterinary MedicineAraçatubaSao PauloBrazil Federal Rural University of Pernambuco(UFRPE) Department of Molecular SciencesRecifePernambucoBrazil Department of Biochemistry University of Nebraska-LincolnLincolnNE68508USA Federal University of Viçosa(UFV) Veterinary DepartmentViçosaMinas GeraisBrazil Federal University of Vale do São Francisco(UNIVASF) Department of Animal SciencePetrolinaPernambucoBrazil University of West Paulista(UNOESTE) Department of AgronomyPresidente PrudenteSao PauloBrazil Federal University of Goiás(UFG) Department of Veterinary MedicineGoiâniaGoiásBrazil

Antibiotic resistance represents a widespread problem in milk *** identification of compounds for a topically applied ointment used in mastitis therapy remains *** from the genus Hymenaea can be administered in cases of multi-drugresistant Staphylococcus aureus infection for ruminant species,but the protective properties are not well *** this research the aim is verify the protective effects of *** against *** infection in bovine mammary epithelial cell line(MAC-T)and to obtain an antioxidant profile evaluation in *** MAC-T cells were challenged with *** after being exposed to the extract of the *** in the protective *** the verification of the viability of the MAC-T cells,the MTT assay was performed,and was used dilutions of the plant extract,starting at 2.5%.The extract of *** was evaluated for antioxidant aspect in different dilutions by FRAP,ORAC and DPPH.A variety of flavonoids(quercetin,luteolin,etc.)have been identified as the main components by using mass spectrometry,reinforcing our in vitro findings that flavonoids,especially quercetin,have a medicinal profile capable of killing mastitis-causing *** excellent antioxidant pattern was observed in the 2.5%solution;however,membrane integrity in MAC-T cells was *** findings suggest low dilutions of *** extract has a desirable protective effect from *** *** in vitro studies can be gleaned upon for further in vivo studies.

关键词： Dairy animal Fitoterapic Hymenaea gender Cell activity Mastitis

Antimicrobial Activity of the Essential Oil from Hyptis carpinifolia Benth

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American Journal of Plant Sciences 2017年第11期8卷 2871-2877页

作者： Karen Caroline Camargo Luís Roberto Batista Paulo Estevao de Souza Maria Luisa teixeira Thaís aparecida Sales Vanuzia Rodrigues Fernandes Ferreira Jéssica Oliveira e Nogueira Maísa Lamounier Magalh?es Alex Rodrigues Silva Caetano David Lee Nelson Maria das Gra?as Cardoso Department of Chemistry Federal University of Lavras Lavras Brazil Department of Food Science Federal University of Lavras Lavras Brazil Department of Phytopathology Federal University of Lavras Lavras Brazil Pro-Rectory of Research and Graduate Studies Federal University of the Jequitinhonha and Mucuri Valleys Diamantina Brazil

Essential oils are secondary metabolites whose biological properties have been studied with emphasis on antimicrobial activity. Hyptis carpinifolia Benth (Rosmaninho) is used in folk medicine in the fight against colds and rheumatism. The objective of this study was to evaluate the antimicrobial activity of the essential oil from H. carpinifolia. The essential oil was extracted by hydrodistillation using a modified Clevenger apparatus. The biological activity was determined using the Agar Cavity Diffusion technique to evaluate the effect of concentrations of 500, 250, 125, 62.5, 31.25, 15.62, 7.81 and 3.9 μg·mL-1 on the bacteria Escherichia coli, Listeria monocytogenes, Salmonella Choleraesuis, Staphylococcus aureus and Pseudomonas aeruginosa. The fungicidal potential was evaluated by the method of dilution in agar, and the percentage inhibition of mycelial growth of Alternaria alternata, Botrytis cinerea and Fusarium oxysporum was evaluated. The dilutions tested were 1000, 750, 500, 250 and 100 μL·L-1. The minimum inhibitory concentrations (MIC) of the essential oil for L. monocytogenes, S. aureus and E. coli were 31.25;15.62 and 62.5 μg·mL-1, respectively. The MIC was 500 μg·mL-1 for Salmonella Choleraesuis and P. aeruginosa. The percentage inhibitions of mycelial growth of B. cinerea and A. alternate were 67% and 33%, respectively, at the dilution of 750 μL·L-1. The essential oil inhibited about 48% of the mycelial growth of F. oxysporum, at the dilution of 1000 μL·L-1. The essential oil of H. carpinifolia presented biological activity against all the microorganisms evaluated.

关键词： Essential Oils Antimicrobial Activity

Theoretical and Comparative Study of the Complex[RuCl_(3)(H_(2)O)_(2)(Gly)]by Density Functional Theory

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Open Journal of Inorganic Chemistry 2018年第1期8卷 43-53页

作者： Katia Meirelles D.de Sousa Marcio Adriano S.Chagas Jesyca Mayra F.D.dos Santos Anderson D.Galvao Fabricio Tarso de Moraes Andressa teixeira Barros Nunes Ribeiro Dario Batista Fortaleza Kelly aparecida da Encarnacao Amorim Wagner B.dos Santos Materials Research Lab Federal University of Mato Grosso—Campus ⅡBarra do GarcasBrazil Institute of Chemistry Brasilia UniversityUNBBrasíliaBrazil

In this work, the use of computational methods was essential to distinguish the three possible isomeric structures of the [RuCl3(H2O)2(Gly)] molecule. The characterization of these molecules was performed using IR, NMR and UV-VIS simulations. Some calculations related to the optimization of structures and properties such as chemical hardness and dipole moment were also conducted. The fac-cis isomer presented promising data when compared to the experimental data, indicating that this is the likely experimentally synthesized isomer. This study demonstrates the technical utility of the computational calculations by virtue of situations that prevent the realization of X-ray diffraction.

关键词： Computational Methods Isomeric Structures Simulations

Chemical Characterization and in Vitro Antitumor Activity of the Essential Oils from the Leaves and Flowers of Callistemon viminalis

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American Journal of Plant Sciences 2015年第16期6卷 2664-2671页

作者： Christiane Maria de Oliveira Maria das Gracas Cardoso Marisa Ionta Marisi Gomes Soares Juliana de Andrade Santiago Guilherme álvaro Ferreira da Silva Maria Luisa teixeira Danúbia aparecida de Carvalho Selvati Rezende Rafaela Vieira de Souza Luana Isac Soares David Lee Nelson Marcos Schleiden Sousa Carvalho Department of Chemistry Federal University of LavrasLavrasBrazil Institute of Biomedical Sciences Federal University of AlfenasAlfenasBrazil Department of Chemistry Federal University of AlfenasAlfenasBrazil Federal University of Vales de Jequitinhonha e Mucuri DiamantinaBrazil Department of Agriculture Federal University of LavrasLavrasBrazil

The objective of this study was to characterize and verify the in vitro antitumor activity of essential oils (EOs) extracted from the leaves and flowers of Callistemon viminalis. The EOs were extracted by hydrodistillation using a modified Clevenger apparatus. The identification and quantification of constituents were performed on a gas chromatograph coupled to a mass spectrometer and a gas chromatograph with a flame ionization detector. The antitumoral activity was evaluated by a colorimetric assay (MTS) using different cell lines derived from human tumors (breast, lung, glioblastoma, and melanoma). The major constituents of the EOs of leaves and flowers were similar, only quantitative differences being observed. The compounds 1,8-cineole, α-pinene and α-terpineol were found in concentrations of 50.4%, 25.8% and 8.7% in the EOs obtained from the leaves and 48.8%, 24.5% and 3.9% in the EOs obtained from the flowers, respectively. The cytotoxic activity of the EOs was observed only in melanoma cultures (HT144). Cultures treated for 48 h with EOs from leaves and flowers (200 μg·mL-1) reduced the viability by 40% and 25%, respectively. Thus, the antiproliferative activity of the EO from leaves was more pronounced than the EO from flowers in cells derived from melanoma.

关键词： Cancer Volatile Oil Escova-de-Garrafa(Bottle Brush)

Clinical Outcomes of Positive Newborns for COVID-19 in Rio de Janeiro, Brazil

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Open Journal of Obstetrics and Gynecology 2021年第12期11卷 1851-1861页

作者： Karina Martins-Cardoso Luana Ferreira Martins de Toledo Juliana aparecida Souza Paz Andressa Rangel de Oliveira Lima Thaís Chrispim de Souza Giangiarulo Jéssica da Silva Oliveira IPB COVID Workgroup Carla Carolina teixeira Jones Zitenfeld Cardia Neto Giovanna Geórgia Pires Carrilho Vallim Shana Priscila Coutinho Barroso Laboratório de Biologia Molecular Instituto de Pesquisas Biomédicas Hospital Naval Marcílio Dias Rio de Janeiro Brasil Instituto de Bioquímica Médica Leopoldo de Meis Universidade Federal do Rio de Janeiro Rio de Janeiro Brasil UTI neonatal Clínica de Pediatria Hospital Naval Marcílio Dias Rio de Janeiro Brasil Serviç o de Patologia Hospital Naval Marcílio Dias Rio de Janeiro Brasil

The disease caused by the SARS-CoV-2 virus has spread very rapidly throughout the world, causing various clinical manifestations and affecting several organs and systems. However, the possibility of vertical transmission of COVID-19 in symptomatic pregnant women has been questioned. The aim of this work was to report cases of possible vertical transmission in pregnant women affected by the SARS-CoV-2 virus, as well as its clinical repercussions on the newborn. In this paper, we conducted a cross-sectional retrospective study by analyzing medical records of pregnant women admitted at the military hospital, in Rio de Janeiro, Brazil, diagnosed with COVID-19 and who had positive newborns. To evaluate epidemiological data, clinical manifestations, laboratorial and histopathological changes were related to such vertical transmission. Only six tested newborns were positive for COVID-19. The mothers had no respiratory symptoms, and the gestational age was at term, except for one newborn born prematurely due to detection of pericadial effusion. Three neonates had respiratory symptoms, requiring ventilatory support. None of the mothers or newborns died. During the pandemic, possible vertical transmission cases of infected pregnant women in the third trimester of pregnancy were observed. According to the case reports published in the literature, they illustrate phenotypes of neonatal involvement with varied clinical manifestations and severity;however, further scientific studies are needed to prove the effective vertical transmission of SARS-CoV-2 in positive pregnant women.

关键词： SARS-CoV-2 Infection Neonatal Outcomes Vertical Transmission