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Electrochemical co-reduction of y(Ⅲ)and Al(Ⅲ)in a fluoride molten salt system and electrolytic preparation of y-Al intermediate alloys

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Journal of Rare Earths 2022年第12期40卷 1945-1952,I0005页

作者： Guoqing yu Lin Zhou Fengqin Liu siming Pang Dehong Chen Hongliang Zhao Zhengping Zuo National Engineering Research Center for Rare Earth Materials General Research Institute for Nonferrous MetalsBeijing 100088China School of Metallurgical and Ecological Engineering University of Science and Technology BeijingBeijing 100083China

In this study,a molten salt co-reduction method was proposed for preparing y-Al intermediate alloys and the electrochemical co-reduction behaviors of y(Ⅲ)and Al(Ⅲ)and the reaction mechanism of intermetallic compound formation were investigated by transient electrochemical *** results show that the reduction of y(Ⅲ)at the Mo electrode is a reversible electrochemical process with a single-step transfer of three electrons,which is controlled by the mass transfer *** diffusion coefficient of y(Ⅲ)in the fluoride salt at a temperature of 1323 K is 5.0238×10^(-3)cm^(2)/***,the thermodynamic properties associated with the formation of y-Al intermetallic compounds were estimated using a steady-state electrochemical method.y-Al intermediate alloy containing 92 wt%yttrium was prepared by constant current electrolysis at 1323 K in the LiF-yF_(3)-AIF_(3)-y_(2)O_(3)(6 wt%)-Al_(2)O_(3)(1 wt%)system at a cathodic current density of 8 A/cm^(2)for 2 *** y-Al intermediate alloy is mainly composed ofα-y2Al and y *** development and application of this innovative technology have solved major technical problems,such as a long production process,high energy consumption,and serious segregation of alloy elements at this stage.

关键词： Intermediate alloy Electrochemical techniques Co-reduction behavior Fluoride salt Rare earths

Failure Mechanisms of Post-Earthquake Bedrock Landslides in Response to Rainfall Infiltration

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Journal of Mountain Science 2011年第1期8卷 96-102页

作者： WU y.ng HE siming LUO yu LI Xinpo Key laboratory of Mountain Hazards and Earth Surface Process Chinese Aeademy of Sciences Chengdu 610041 China Institute of Mountain Hazards and Environment Chinese Academy of Sciences Chengdu 610041 China

Among the triggering factors of postearthquake bedrock landslides, rainfall plays an important role. However, with slope variation, the mechanism of its effects on the failure of rock landslides is not dear. Here, from the viewpoint of fracture mechanics, and based on post-earthquake conditions, the mechanisms of crack propagation, water infiltration and development of the sliding surface were investigated. Then, according to the upper boundary theorem, the effects of water infiltrated into fractures on the stability of rock slopes were analyzed quantitatively. Finally, an example is presented to verify the theory. The results show that the propagation and coalescence of cracks and the lubrication of incipient sliding surfaces are the main causes of the failure of post-earthquake rock landslides in response to rainfall.

关键词： Post-earthquake Fracture infiltration Fracture mechanics Propagation and coalescence Upper boundary theorem Energy theory Rock landslide

A loss-of-function mutant allele of a glycosyl hydrolase gene has been co-opted for seed weight control during soybean domestication

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Journal of Integrative Plant Biology 2023年第11期65卷 2469-2489页

作者： siming Wei Bin y.ng Hongwei Jiang Zhenghong An yan Wang Bingbing Li Ce y.ng Weiwei Zhu Qingshan Chen Chaoy.ng He State Key Laboratory of Plant Diversity and Specialty Crops/State Key Laboratory of Systematic and Evolutionary Botany Institute of Botanythe Chinese Academy of SciencesBeijing 100093China China National Botanical Garden Beijing 100093China College of Life Sciences University of Chinese Academy of SciencesBeijing 100049China College of Agriculture Northeast Agricultural UniversityHarbin 150030China Jilin Academy of Agricultural Sciences Changchun 130022China The Innovative Academy of Seed Design the Chinese Academy of SciencesBeijing 100101China

The resultant DNA from loss-of-function mutation can be recruited in biological evolution and ***,we present such a rare and potential case of“to gain by loss”as a neomorphic mutation during soybean domestication for increasing seed *** a population derived from a chromosome segment substitution line of Glycine max(SN14)and Glycine soja(ZyD06),a quantitative trait locus(QTL)of 100-seed weight(q HSW)was mapped on chromosome 11,corresponding to a truncatedβ-1,3-glucosidase(βGlu)*** novel gene hsw results from a 14-bp deletion,causing a frameshift mutation and a premature stop codon in theβ*** contrast to HSW,the hsw completely lostβGlu activity and function but acquired a novel function to promote cell expansion,thus increasing seed *** hsw instead of HSW produced large soybean seeds,and surprisingly,truncating hsw via gene editing further increased the seed *** further found that the core 21-aa peptide of hsw and its variants acted as a promoter of seed *** variation in these transgenic soybean lines substantiated the integration hsw into cell and seed size ***,the hsw allele underwent selection and expansion during soybean domestication and *** work cloned a likely domesticated QTL controlling soybean seed weight,revealed a novel genetic variation and mechanism in soybean domestication,and provided new insight into crop domestication and breeding,and plant evolution.

关键词： domestication frameshift mutation QTL seed weight soybean to gain by loss

Construction of eukaryotic expression vector of humanarresten gene and its secreted expression in HEK 293 cells

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Frontiers of Medicine 2009年第3期3卷 297-302页

作者： Wei LI Zifang SOng Qichang ZHEng Jun XIOng Dan SHAng siming GUAN Xiaogang SHU Department of Gerontology Union HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430022China Department of General Surgery Union HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430022China

The eukaryotic expression vector of human arresten gene was constructed and its secretive expression in human embryonic kidney(HEK 293)cells was *** arresten gene was ampliﬁed from recombinant plasmid pGEM-Arr by polymerase chain reaction(PCR),and then digested with restriction endonucleases BamH I and EcoR *** target fragment was inserted into the BamH I and EcoR I restriction sites of eukaryotic expression vector pSecTag2 to construct ***-tion analysis and DNA sequencing indicated that the arresten gene was successfully inserted into *** recombinant plasmid was subsequently transfected into HEK 293 cells with LipofectAMINETM2000 Reagent,and the expression of the target gene was *** transcription PCR(RT-PCR)revealed that the mRNA of the target gene was transcribed in the transfected HEK 293 *** blot analysis veriﬁed that the recombinant protein in supernatants was *** supernatants of transfected cells were prepared,and 3-(4,5)-dimethylthiazol(-2-y1)-3,5-di-phenyltetrazoliumbromide(MTT)assay was carried out to assess their effects on the proliferation of human umbilical vein endothelial cells,which showed that the recombinant protein could signiﬁcantly suppress the proliferation of human umbilical vein endothelial cells in *** results provided a solid foundation to explore the usage of arresten in tumor anti-angiogenic gene therapy.

关键词： angiogenesis inhibitor arresten eukaryotic expression HEK 293 cells endothelial cells

Bile acid signaling and bariatric surgery

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Liver Research 2017年第4期1卷 208-213页

作者： Jingyan Tian Silvia Huang siming Sun Lili Ding Eryun Zhang yanjun Liu Wendong Huang National Clinical Research Center for Endocrine and Metabolic Diseases Shanghai Institute of Endocrine and Metabolic DiseasesDepartment of Endocrinology and MetabolismRuijin HospitalShanghai Jiao Tong University School of MedicineShanghaiChina Department of Diabetes Complications and Metabolism Beckman Research Institute of City of HopeDuarteCAUSA Eugene Robert Summer Program City of HopeDuarteCAUSA Shanghai Key Laboratory of Compound Chinese Medicines and the Ministry of Education(MOE)Key Laboratory of Standardization of Chinese Medicines Institute of Chinese Materia MedicaShanghai University of Traditional Chinese MedicineShanghaiChina

The rapid worldwide rise in obesity rates over the past few decades imposes an urgent need to develop effective strategies for treating obesity and associated metabolic *** surgical procedures,such as Roux-en-y gastric bypass(RyGB)and vertical sleeve gastrectomy(VSG),currently provide the most effective treatment for obesity and type 2 diabetes(T2D),as well as for non-alcoholic steatohepatitis(NASH).However,the underly.ng mechanisms of the beneficial effects of bariatric surgery remain *** studies have identified bile acids as potential signaling molecules involved in the beneficial effects of bariatric *** review focuses on the most recent studies on the roles of bile acids and bile acid receptors Farnesoid X receptor(FXR)and G protein-coupled bile acid receptor 5(TGR5)in bariatric *** also discuss the possibility of modulating bile acid signaling as a pharmacological therapeutic approach to treating obesity and its associated metabolic complications.

关键词： Bile acid(BA) Bariatric surgery Farnesoid X receptor(FXR) G protein-coupled bile acid receptor 5 (TGR5) Microbiota

Analysis on Sequence and Elements of Cucumisin Promoter

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Agricultural Biotechnology 2017年第2期6卷 19-21,44页

作者： siming HOU Lingyun LIU Xiaohong y.ng Department of Life Science and Technology Kunming University

Abstract Leaves of melon were collected and extracted by the CTAB method for total DNA which was used for PCR amplification, obtaining the gene sequence of cucumisin promoter. The sequence results were processed and analyzed with DNAman, DNAstar and other softwares, and bioinformatic element analysis was performed with PlantCARE and PLACE. The analysis results showed that the cucumisin promoter shared 100%, 99% and 99% homology with Ay055805, LN713264 and LN681897, respectively. The promoter sequence contains a variety of c/s-acting elements common in fruit promoters of higher plants such as TATA-Box and CAAT-Box, and light-responsive elements, some of which involved in ABA and VP1 responsiveness and salicylic acid responsiveness. This study provides a scien- tific basis for further research on genetic engineering of fruits.

关键词： Fruit promoter Sequence analysis Melon gene Bioinformatics analysis Cis-acting elements

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预应力锚杆作用机制研究

岩石力学与工程学报 2006年第9期25卷 1876-1880页

作者：何思明李新坡中国科学院成都山地灾害与环境研究所四川成都610041

针对预应力锚杆作用机制研究方面存在的问题,根据弹性理论轴对称问题的基本理论和方法,在引入锚杆影响范围内岩体平均轴向应力(平均轴向应变)假设的条件下,推导锚杆在完全黏结条件下界面剪应力分布公式;以此为基础,在考虑界面本构关系... 详细信息

针对预应力锚杆作用机制研究方面存在的问题,根据弹性理论轴对称问题的基本理论和方法,在引入锚杆影响范围内岩体平均轴向应力(平均轴向应变)假设的条件下,推导锚杆在完全黏结条件下界面剪应力分布公式;以此为基础,在考虑界面本构关系情况下,研究界面脱黏段的剪应力分布及脱黏段长度,阐述预应力锚杆的荷载传递特性,从而为预应力锚杆设计提供理论依据。

关键词：基础工程轴对称问题预应力锚杆作用机制

具有无穷时滞的中立型积分微分系统的平稳振荡

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应用数学学报 2005年第3期28卷 536-545页

作者：彭世国朱思铭广东工业大学应用数学系广州510090 中山大学数学系广州510275

利用重合度理论中的延拓定理和微分积分不等式讨论具有无穷时滞的中立型积分微分系统其中x(t)=(x1(t),…,xn(t))T,G∈C2(Rn,R),f∈C(R×R×Rn×Rn,Rn),e∈C(R,Rn),e(t+ω)≡e(t),f(t+ω,u+ω,x,y)≡f(t,u,x,y),f(t,u,0,0)≡0,t,u∈R,x,y... 详细信息

关键词：无穷时滞中立型积分微分系统周期解全局渐近稳定平稳振荡

Construction of Lactuca sativa Plastid Transformation Vector and High-level Expression of gfp Gene in Escherichia coli

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Agricultural Biotechnology 2016年第4期5卷 1-4页

作者： siming HOU Liy.ng ZHOU Lulu BU Chunlei y.ng Ting GAO Tian TIAN Zheng'an y.ng Department of Life Science and Technology Kunming University College of Horticulture and Landscape Yunnan Agricultural University Agrotechnical Center Station Agricultural Bureau of Qiaojia County

Using genomic DNA of bolting-tolerant lettuce as a template,flanking fragments of lettuce plastid rpo A gene were amplified and cloned by PCR. Targeting the sites of these two fragments,homologous recombinant fragments of exogenous gene were integrated to construct lettuce plastid expression vector p Brpo AGFP,which harbored the expression cassette Prrn-gfp-aad A-Tpsb A. The results showed that the amplified flanking fragments were 1.2 and 1.1 kb in size. After sequencing,restriction digestion,ligation and transformation,lettuce plastid expression vector containing expression cassette Prrn-gfp-aad A-Tpsb A was constructed and confirmed by SDS-PAGE electrophoresis. The results of SDS-PAGE electrophoresis indicated that gfp gene was efficiently expressed under the regulation of plasmid specific promoter Prrn and terminator Tpsb A. GFP accounted for 45. 6% of total soluble proteins; inclusion bodies accounted for 47.5 % of bacterial proteins,which reached relatively high expression levels. The construction of lettuce plastid expression vector p Brpo A-GFP laid a solid foundation for establishment of subsequent lettuce plastid transformation system and genetic improvement of lettuce using various functional genes.

关键词： Lactuca sativa var. capitata L. Plastid Expression vector gfp gene High-level expression