检索结果-南通市图书馆

Molten metal-organic complex to synthesize versatile ultrathin nonlayered oxides

在线全文

学校读者我要写书评

暂无评论

Nano Research 2024年第4期17卷 3147-3155页

作者： Kaisi Liu Jiabin Wu Qun Li Hongrun Jin Yongxin Luo Bei Qi simin Dai Jianquan zhao Liang Huang Jun Zhou Hubei Key Laboratory of Biomass Fibers and Eco-Dyeing&Finishing Wuhan Textile UniversityWuhan 430200China Wuhan National Laboratory for Optoelectronics School of Optical and Electronic InformationHuazhong University of Science and TechnologyWuhan 430074China Analytical and Testing Center Huazhong University of Science and TechnologyWuhan 430074China

Two-dimensional(2D)materials have attracted a great deal of research interest because of their unique electrical,magnetic,optical,mechanical,and catalytic properties for various applications.To date,however,it is still difficult to fabricate most functional oxides as 2D materials unless they have a layered structure.Herein,we report a one-step universal strategy for preparing versatile non-layered oxide nanosheets by directly annealing the mixture of metal nitrate and dimethyl imidazole(2-MI).The 2-MI plays the key role for 2D oxides since 2-MI owns a very low molten point and sublimation temperature,in which its molten liquid can coordinate with metal ions,forming a metal-organic framework,and easily puffing by its gas molecules.A total of 17 materials were prepared by this strategy,including non-layered metal oxide nanosheets as well as metal/metal oxide loaded nitrogen-doped carbon nanosheets.The as-prepared cobalt particle-loaded nitrogen-doped carbon nanosheets(Co@N/C)exhibit remarkable bifunctional oxygen reduction reaction(ORR)and oxygen evolution reaction(OER)electrocatalytic activity and durability.Besides,the Zn-air battery utilizing a Co@N/C catalyst exhibits high power density of 174.3 mW·cm^(-2).This facile strategy opens up a new way for large-scale synthesis of 2D oxides that holds great potential to push 2D oxides for practical applications.

关键词： non-layered oxide nanosheet metal/metal oxide@N/C oxygen evolution reaction(OER) oxygen reduction reaction(ORR) Zn-air battery

Deubiquitinase ubiquitin-specific protease 3 (USP3) inhibits HIV-1 replication via promoting APOBEC3G (A3G) expression in both enzyme activity-dependent and -independent manners

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Chinese Medical Journal 2022年第22期135卷 2706-2717页

作者： simin zhao Baisong Zheng Liuli Wang Wenzhe Cui Chunlai Jiang Zhuo Li Wenying Gao Wenyan Zhang Center for Pathogen Biology and Infectious Diseases Institute of Virology and AIDS ResearchKey Laboratory of Organ Regeneration and Transplantation of The Ministry of EducationThe First Hospital of Jilin UniversityChangchunJilin 130021China College of Life Science of Jilin University ChangchunJilin 130012China Department of Regenerative Medicine School of Pharmaceutical SciencesJilin UniversityChangchunJilin 130012China Jilin Provincial Key Laboratory on Molecular and Chemical Genetics The Second Hospital of Jilin UniversityChangchunJilin 130041China Department of Endocrinology and Metabolism The First Hospital of Jilin UniversityChangchunJilin 130021China

Background: Ubiquitination plays an essential role in many biological processes, including viral infection, and can be reversed by deubiquitinating enzymes (DUBs). Although some studies discovered that DUBs inhibit or enhance viral infection by various mechanisms, there is lack of information on the role of DUBs in virus regulation, which needs to be further investigated.Methods: Immunoblotting, real-time polymerase chain reaction,in vivo/in vitro deubiquitination, protein immunoprecipitation, immunofluorescence, and co-localization biological techniques were employed to examine the effect of ubiquitin-specific protease 3 (USP3) on APOBEC3G (A3G) stability and human immunodeficiency virus (HIV) replication. To analyse the relationship between USP3 and HIV disease progression, we recruited 20 HIV-infected patients to detect the levels of USP3 and A3G in peripheral blood and analysed their correlation with CD4^(+) T-cell counts. Correlation was estimated by Pearson correlation coefficients (for parametric data).Results: The results demonstrated that USP3 specifically inhibits HIV-1 replication in an A3G-dependent manner. Further investigation found that USP3 stabilized 90% to 95% of A3G expression by deubiquitinating Vif-mediated polyubiquitination and blocking its degradation in an enzyme-dependent manner. It also enhances the A3G messenger RNA (mRNA) level by binding to A3G mRNA and stabilizing it in an enzyme-independent manner. Moreover, USP3 expression was positively correlated with A3G expression (r= 0.5110) and CD4^(+) T-cell counts (r= 0.5083) in HIV-1-infected patients.Conclusions: USP3 restricts HIV-1 viral infections by increasing the expression of the antiviral factor A3G. Therefore, USP3 may be an important target for drug development and serve as a novel therapeutic strategy against viral infections.

关键词： APOBEC3G Ubiquitin-specific protease 3 Deubiquitination Human immunodeficiency virus-1 Vif Human immunodeficiency virus Deubiquitinase

Identification of the candidate gene controlling tiller angle in common wheat through genome-wide association study and linkage analysis

在线全文

学校读者我要写书评

暂无评论

The Crop Journal 2023年第3期11卷 870-877页

作者： Lei zhao Canguan Wang Tongzhu Wang Jinyuan Liu Qi Qiao Yulu Yang Pengyu Hu Leilei Zhang simin zhao Daiying Chen Yan Ren Ning Zhang Zhongdong Dong Feng Chen National Key Laboratory of Wheat and Maize Crop Science/CIMMYT-China Wheat and Maize Joint Research Center/Agronomy College Henan Agricultural UniversityZhengzhou 450046HenanChina

Wheat tiller angle(TA)is an important agronomic trait that contributes to grain production by affecting plant architecture.It also plays a crucial role in high-yield wheat breeding.An association panel and a recombinant inbred line(RIL)population were used to map quantitative trait loci(QTL)for TA.Results showed that 470 significant SNPs with 10.4%–28.8%phenotypic variance explained(PVE)were detected in four replicates by a genome-wide association study(GWAS).Haplotype analysis showed that the TA_Hap_4B1 locus on chromosome 4B was a major QTL to regulate wheat TA.Ten QTL were totally detected by linkage mapping with the RIL population,and QTA.hau-4B.1 identified in six environments with the PVE of 7.88%–18.82%was a major and stable QTL.A combined analysis demonstrated that both TA_Hap_4B1 and QTA.hau-4B.1 were co-located on the same region.Moreover,QTA.hau-4B.1 was confirmed by bulked segregant RNA-Seq(BSR-Seq)analysis.Phenotypic analysis showed that QTA.hau-4B.1was also closely related to yield traits.Furthermore,Traes CS4B02G049700 was considered as a candidate gene through analysis of gene sequence and expression.This study can be potentially used in cloning key genes modulating wheat tillering and provides valuable genetic resources for improvement of wheat plant architecture.

关键词： Common wheat Tiller angle GWAS Linkage mapping Plant architecture

Novel Graphene Biosensor Based on the Functionalization of Multifunctional Nano-bovine Serum Albumin for the Highly Sensitive Detection of Cancer Biomarkers

在线全文

学校读者我要写书评

暂无评论

Nano-Micro Letters 2019年第2期11卷 24-36页

作者： Lin Zhou Kun Wang Hao Sun simin zhao Xianfeng Chen Dahong Qian Hongju Mao Jianlong zhao State Key Laboratory of Transducer Technology Key Laboratory of Terahertz Solid-State TechnologyShanghai Institute of Microsystem and Information TechnologyChinese Academy of SciencesShanghai 200050People’s Republic of China School of Biomedical Engineering Shanghai Jiao Tong UniversityShanghai 200240People’s Republic of China School of Electronic Engineering Bangor UniversityBangor LL57 1UTUK

A simple,convenient,and highly sensitive bio-interface for graphene field-effect transistors(GFETs) based on multifunctional nano-denatured bovine serum albumin(nano-dBSA) functionalization was developed to target cancer bio-markers.The novel graphene–protein bioelectronic interface was constructed by heating to denature native BSA on the graphene substrate surface.The formed nano-d BSA film served as the cross-linker to immobilize monoclonal antibody against car-cinoembryonic antigen(anti-CEA mAb) on the graphene channel activated by EDC and Sulfo-NHS.The nano-dBSA film worked as a self-protecting layer of graphene to prevent surface contamination by lithographic processing.The improved GFETbiosensor exhibited good specificity and high sensitivity toward the target at an ultralow concentration of 337.58 fg mL-1.The electrical detection of the binding of CEA followed the Hill model for ligand–receptor interaction,indicating the negative binding cooperativity between CEA and anti-CEA mAb with a dissociation constant of 6.82×10-10M.The multifunctional nano-dBSA functionalization can confer a new function to graphene-like 2D nanomaterials and provide a promising bio-functionalization method for clinical application in biosensing,nanomedicine,and drug delivery.

关键词： Bio-interface Multifunctional denatured BSA GFET biosensor Cancer biomarker

40例ANCA相关性血管炎耳部受累临床特征分析

在线全文

学校读者我要写书评

暂无评论

临床耳鼻咽喉头颈外科杂志 2024年第3期38卷 183-187页

作者：刘日渊廖思敏赵玉荣朱剑杨仕明赵辉解放军总医院第六医学中心耳鼻咽喉头颈外科医学部、国家耳鼻咽喉疾病临床医学研究中心、解放军耳鼻咽喉研究所聋病教育部重点实验室、聋病防治北京市重点实验室北京100048 解放军总医院第一医学中心风湿免疫科

目的:分析抗中性粒细胞胞浆抗体(anti-neutrophil cytoplasmic antibody,ANCA)相关性血管炎耳部受累的临床特点及治疗预后。方法:回顾性分析解放军总医院第一医学中心诊治的40例ANCA相关性血管炎伴耳部症状患者的临床表现、实验室检查... 详细信息

目的:分析抗中性粒细胞胞浆抗体(anti-neutrophil cytoplasmic antibody,ANCA)相关性血管炎耳部受累的临床特点及治疗预后。方法:回顾性分析解放军总医院第一医学中心诊治的40例ANCA相关性血管炎伴耳部症状患者的临床表现、实验室检查、纯音测听、声导抗检查、颞骨CT及治疗预后。结果:以耳部表现为首发症状者11例(27.5%),其中最常见的耳部症状为听力下降,其余有耳闷、耳溢液以及耳鸣。40例患者中,有35例出现听力下降,传导性耳聋19例(47.5%)、感音神经性耳聋9例(22.5%)、混合性耳聋7例(17.5%);5例患者仅有耳闷、耳鸣,听力检查无异常(12.5%)。40例患者均有多器官受累,最常见于呼吸系统;且ANCA检查均阳性;治疗包括全身使用糖皮质激素、免疫抑制剂、生物制剂。经治疗后,3例听力恢复正常且无波动(7.5%),22例症状减轻(55.0%),6例听力反复波动(15.0%),9例无明显改善(22.5%)。结论:ANCA相关性血管炎中耳炎(otitis media with AAV,OMAAV)疾病改变是动态过程:传导性耳聋(分泌性中耳炎)可以是前期表现,后期可出现双耳混合性耳聋。耳鼻喉科医生在诊治分泌性中耳炎反复发作的患者时,需考虑OMAAV诊断,多系统症状问诊及ANCA检查可帮助鉴别,早期全身用药及免疫抑制剂或生物制剂的应用可帮助患者听力恢复。

关键词：抗中性粒细胞胞浆抗体相关性血管炎中耳炎

Determination of 6258-70,a new semi-synthetic taxane,in rat plasma and tissues:Application to the pharmacokinetics and tissue distribution study

在线全文

学校读者我要写书评

暂无评论

Journal of Pharmaceutical Analysis 2016年第4期6卷 219-225页

作者： simin zhao Yuanyuan Zhang Ping Ju Liqiang Gu Rui Zhuang Longshan zhao Xing Tang Kaishun Bi Xiaohui Chen School of Pharmacy Shenyang Pharmaceutical University

Cancer is the leading cause of death all over the world.Among the chemotherapy drugs,taxanes play an important role in cancer treatment.6258-70 is a new semi-synthetic taxane which has a broad spectrum of antitumor activity.A fast and reliable high performance liquid chromatography-tandem mass spectrometry（HPLC-MS/MS） method was developed for quantification of 6258-70 in rat plasma and tissues in this paper.After extraction by liquid-liquid extraction method with methyl tert-butyl ether,the samples were separated on a Kinetex C_（18） column（50 mm × 2.1 mm,2.6 μm,Phenomenex,USA） within3 min.The method was fully validated with the matrix effect between 87.7%and 99.5%and the recovery ranging from 80.3%to 90.1%.The intra- and inter-day precisions were less than 9.5%and the accuracy ranged from-3.8%to 6.5%.The reliable method was successfully applied to the pharmacokinetics and tissue distribution studies of 6258-70 after intravenous administration in rats.The pharmacokinetic results indicated that the pharmacokinetic behavior of 6258-70 in rats was in accordance with linear features within tested dosage of 1 to 4 mg/kg,and there was no significant difference between the two genders.The tissue distribution study showed that 6258-70 had an effective penetration,spread widely and rapidly and could cross blood-brain barrier.The results of pharmacokinetics and tissue distribution may provide a guide for future study.

关键词： 6258-70 HPLC-MS/MS Pharmacokinetics Tissue distribution

Acetylshikonin inhibits inflammatory responses and Papain-like protease activity in murine model of COVID-19

在线全文

学校读者我要写书评

暂无评论

Signal Transduction and Targeted Therapy 2022年第11期7卷 4028-4030页

作者： Ning Lu Tingxuan Gu Xueli Tian simin zhao Guoguo Jin Fredimoses Mangaladoss Yan Qiao Kangdong Liu Ran zhao Zigang Dong Department of Pathophysiology School of Basic Medical SciencesAcademy of Medical ScienceCollege of MedicineZhengzhou UniversityZhengzhouHenan450001P.R.China China-US(Henan)Hormel Cancer Institute No.127Dongming RoadJinshui DistrictZhengzhouHenan450008China Department of Pathology The Affiliated Cancer Hospital of Zhengzhou UniversityZhengzhouHenan450008China The Henan Luoyang Orthopedic Hospital ZhengzhouHenanP.R.China

Dear Editor,The emerging severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)variant Omicron has rapidly replaced the Delta variant and presents a huge challenge to public health and health care infrastructure.Cytokine-driven hyperinflammation is the leading cause of a series of COVID-19 clinical symptoms.1 Our research attempted to discover an anti-inflammatory or anti-viral small molecule agent capable of treating COVID-19 hyperinflammation.

关键词： inflammatory acute inflammation

中国网络基础设施建设的碳减排效应研究--基于“宽带中国”战略的准自然实验

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

生态经济 2024年第5期40卷 28-38页

作者：张优智刘寅可赵璟温思敏西安石油大学经济管理学院陕西西安710065 西安理工大学经济与管理学院陕西西安710054

当前中国经济已进入高质量发展阶段,节能减排已成为经济高质量发展的内在要求。论文立足数字经济时代背景,基于2006—2019年中国271个地级市面板数据,采用倾向得分匹配与双重差分法相结合来分析“宽带中国”战略对碳减排效应的影响。研... 详细信息

当前中国经济已进入高质量发展阶段,节能减排已成为经济高质量发展的内在要求。论文立足数字经济时代背景,基于2006—2019年中国271个地级市面板数据,采用倾向得分匹配与双重差分法相结合来分析“宽带中国”战略对碳减排效应的影响。研究发现:(1)“宽带中国”战略显著促进了碳减排效应,但是空间效应分析发现“宽带中国”战略的实行提高了相邻城市的二氧化碳排放量。(2)区域异质性分析发现,“宽带中国”战略在促进碳减排效应的同时增加了成熟型城市和中国西部城市的二氧化碳排放量。(3)城市产业结构和技术创新是“宽带中国”战略影响碳减排效应的重要途径。

关键词：网络基础设施建设 “宽带中国”战略碳减排双重差分

利妥昔单抗治疗系统性红斑狼疮肠道受累10例临床分析

在线全文

学校读者我要写书评

暂无评论

中华内科杂志 2024年第2期63卷 198-202页

作者：赵玉荣赵征张洁李坤鹏杨金水孙飞廖思敏张江林黄烽朱剑解放军总医院第一医学中心风湿免疫科北京100853

回顾性分析2015年1月至2023年1月在解放军总医院第一医学中心风湿免疫科住院治疗的10例接受利妥昔单抗(RTX)治疗的系统性红斑狼疮(SLE)肠道受累患者的疗效和不良反应。10例患者中,男2例,女8例,年龄(41.9±8.8)岁,发病年龄(28.8±9.2)岁... 详细信息

回顾性分析2015年1月至2023年1月在解放军总医院第一医学中心风湿免疫科住院治疗的10例接受利妥昔单抗(RTX)治疗的系统性红斑狼疮(SLE)肠道受累患者的疗效和不良反应。10例患者中,男2例,女8例,年龄(41.9±8.8)岁,发病年龄(28.8±9.2)岁,诊断SLE的总病程为(109.6±59.9)个月,诊断SLE肠道受累的病程为(89.3±50.2)个月,出现肠道症状至明确诊断SLE肠道受累的时间为1.5(1.0,8.0)个月,诊断SLE肠道受累至使用RTX的时间为13.0(1.0,46.3)个月,应用RTX治疗后随访时间(55.3±28.4)个月。10例患者中,腹痛5例,腹胀4例,腹泻9例,神经系统受累3例,狼疮肾炎9例,浆膜炎7例。10例患者均行腹部CT、腹部X线平片检查,表现为肠壁水肿8例,肠管扩张7例,靶征4例,肠系膜血管充血3例,肠系膜脂肪密度增加8例,假性肠梗阻6例。低补体C3者10例(补体C3250~750 mg/L);低补体C4者9例(补体C410~90 mg/L)。10例患者SLE疾病活动性指数2000(SLEDAI-2K)基线期为20.5(17.8,30.0)。10例患者接受RTX(0.5 g,第1天、第14天,或375 mg/m 2,第1天、第14天)诱导治疗后,肠道症状完全缓解。4例患者发生不良反应,其中3例同时接受了大剂量糖皮质激素冲击联合RTX治疗,不良反应主要表现为1例IgG减低和单纯疱疹病毒感染,1例IgG减低和肺部感染,1例肺部感染,1例IgG减低。对难治性SLE肠道受累者,RTX可能是有效治疗策略之一。

关键词：红斑狼疮,系统性肠道受累利妥昔单抗靶征不良反应