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Exploring conservative management for cervical intraepithelial neoplasia grade 2 in organised cervical cancer screening programmes:a multicentre study in Italy

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Family Medicine and Community Health 2024年第S2期12卷 1-7页

作者： silvia Gori helena Frayle Alessio Pagan Marika Soldà Cesare Romagnolo Egle Insacco Licia Laurino Mario Matteucci Giuseppe Sordi Enrico Busato Manuel Zorzi Tiziano Maggino Annarosa Del Mistro CIN2 study Working Group Veneto Institute of Oncology IOV-IRCCS PadovaItaly Local Health Unit Marca Trevigiana TrevisoItaly Local Health Unit Serenissima VeneziaItaly Obstetrics and Gynecology Azienda Ospedale UniversitàPadovaItaly Local Health Unit Scaligera VeronaItaly Veneto Tumour Registry Azienda ZeroPadovaItaly 不详

Cervical intraepithelial neoplasia grade 2(CIN2)lesions may regress spontaneously,offering an alternative to immediate treatment,especially for women of childbearing age(15–45 years).We conducted a prospective multicentre study on conservative CIN2 management,with semiannual follow-up visits over 24 months,biomarkers’investigation and treatment for progression to CIN3+or CIN2 persistence for more than 12 ***,we assess women’s willingness to participate and adherence to the study *** study was set in population-based organised cervical cancer *** April 2019 to October 2021,640 CIN2 cases were diagnosed in women aged 25–64 participating in the screening *** to our predefined inclusion and exclusion criteria,228(35.6%)women were not eligible;93(22.6%)of the 412 eligible refused,and 319(77.4%)were *** for personal reasons(ie,desire to become pregnant,anxiety,difficulty in complying with the study protocol)and external barriers(ie,residence elsewhere and language problems)accounted for 71%and 17%,*** 9%expressed a preference for *** primary ineligibility factor was the upper age limit of 45 *** enrolment,12(4%)women without evidence of progression requested treatment,125(39%)were lost to follow-up(mostly after 6–12 months)and 182(57%)remained ***,40%of enrolees did not fully adhere to the protocol,whereas only 5%(20/412)of the eligible women desired *** study demonstrates a good acceptance of conservative management for CIN2 lesions by the women,supporting its implementation within cervical screening programmes.

关键词： cervical conservative centre

Strigolactone Analogs as Molecular Probes in Chasing the （SLs） Receptor/s： Design and Synthesis of Fluorescent Labeled Molecules

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Molecular Plant 2013年第1期6卷 113-127页

作者： Cristina Prandi helena Rosso Beatrice Lace Ernesto G. Occhiatot Alberto Oppedisano silvia Tabasso Gabriele Alberto Marco Blangetti Dipartimento di Chimica-Universita di Torino via R Giuria 7 10127 Torino Italy Dipartimento di Scienze della Vita e Biologia dei Sistemi Viale Mattioli 25 10125 Torino Italy Dipartimento di Chimica 'Ugo Schiff' Universita di Firenze Via della Lastruccia 13 50019 Sesto Fiorentino (FI) Italy School of Chemistry and Chemical Biology University College Dublin Belfield Dublin 4 Ireland

Originally identified as allelochemicals involved in plant-parasite interactions, more recently, Strigolactones （SLs） have been shown to play multiple key roles in the rhizosphere communication between plants and mycorrhizal fungi. Even more recent is the hormonal role ascribed to SLs which broadens the biological impact of these relatively simple molecules. In spite of the crucial and multifaceted biological role of SLs, there are no data on the receptor（s） which bind（s） such active molecules, neither in the producing plants nor in parasitic weeds or AM fungi. Information about the putative receptor of SLs can be gathered by means of structural, molecular, and genetic approaches. Our contribution on this topic is the design and synthesis of fluorescent labeled SL analogs to be used as probes for the detection in vivo of the receptor（s）. Knowledge of the putative receptor structure will boost the research on analogs of the natural substrates as required for agricultural applications.

关键词： strigolactones parasitic weeds mycorrhizal fungi receptor fluorescence.

Liquefaction of Kraft Lignin at Atmospheric Pressure

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Journal of Renewable Materials 2019年第6期7卷 527-534页

作者： silvia helena Fuentes da Silva Patricia Soares Bilhalva dos Santos Darci Alberto Gatto Maria Angeles Andres Itziar Egüés Chemical and Environmental Engineering Department University of Basque CountryPlaza Europa 120018Donostia-San SebastiánSpain Faculty of Forestry Engineering Federal University of ParáC.JoséPorfirio2515AltamiraBrazil College of Material Engineering(PPGCEM) Federal University of PelotasBrazil

Kraft lignin was liquefied using polyethylene glycol#400(PEG)and glycerol(G)in a weight ratio of 80/20(w/w)and sulphuric acid(SA)as catalyst under atmospheric pressure at 160º*** three independent variables:reaction time(60,80 and 100 min),percentage of lignin(15,20 and 25%,w/w),and catalyst concentration(0,3 and 6%,w/w),were varied resulting in 27 experimental *** effect of these reaction conditions on the properties of the polyols was *** statistical analysis showed that only“the percentage of lignin”did not influence the properties of the liquefied products,however,reaction time and catalyst load were important *** resulting liquefied products were characterized by FTIR analysis.

关键词： Biopolyols Kraft lignin liquefaction renewable resource thermochemical conversion

Characterization of Bovine and Ovine WPC Obtained by Different Membrane Configuration Processes

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Journal of Chemistry and Chemical Engineering 2011年第4期5卷 316-324页

作者： Marta helena Fernandes henriques Carlos Jose Dias Pereira Maria helena Mendes Gil Department of Food Science and Technology Polytechnic Institute of Coimbra Escola Superior Agraria Bencanta Coimbra 3040-316 Portugal Chemical Engineering Department Faculty of Science and Technology University of Coimbra Rua Silvio Lima - Polo II Coimbra 3030- 790 Portugal

The objective of this stud）, was to valorize bovine and ovine cheese whey resulting from small and medium cheese manufacture plants, by producing liquid and dr）＂ whey protein concentrates （LWPC and WPC）. The flexibility implemented by batch ultrafiltration （UF） and diafiltration （DF） allowed for the production and ovine WPC with 61 -87% （dry basis）. Diatiltration, performed in of liquid bovine WPC with 43-66% protein contents （dry basis） sequential dilution mode （DFsdm） did not improve significantly the composition of WPC liquid products comparing to the results achieved by conventional UF. However, using DF in volume reduction mode （DFvrm） protein content was increased more than 20% comparing to conventional UF. Ovine products showed higher protein levels （62-84% dry basis） what makes their manufacture attractive. Protein profiles varied with the whey origin and with the concentration process. Using batch DFvrm was possible to obtain richer protein products free of low MW compounds.

关键词： Whey proteins whey protein concentrates (WPC) ovine WPC ultrafiltration diafiltration

What exists beyond cag A and vacA? Helicobacter pylori genes in gastric diseases

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World Journal of Gastroenterology 2015年第37期21卷 10563-10572页

作者： Débora Menezes da Costa Eliane dos Santos Pereira silvia helena Barem Rabenhorst Molecular Genetics Laboratory Department of Pathology and Forensic Medicine School of Medicine Federal University of Ceará

Helicobacter pylori(H. pylori) infection is present in more than half the world's population and has been associated with several gastric disorders, such as gastritis, peptic ulceration, and gastric *** clinical outcome of this infection depends on host and bacterial factors where H. pylori virulence genes seem to play a relevant role. Studies of cag A and vac A genes established that they were determining factors in gastric pathogenesis. However, there are gastric cancer cases that are cag A-negative. Several other virulence genes have been searched for, but these genes remain less well known that cag A and vac A. Thus, this review aimed to establish which genes have been suggested as potentially relevant virulence factors for H. pylori-associated gastrointestinal diseases. We focused on the cag-pathogenicity island, genes with adherence and motility functions, and ice A based on the relevance shown in several studies in the literature.

关键词： Helicobacter pylori Virulence genes Cagpathogenici

A nano-enhanced vaccine for metastatic melanoma immunotherapy

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Cancer Drug Resistance 2022年第3期5卷 829-845页

作者： Katelyn E.Salotto Walter C.Olson Jr Karlyn E.Pollack Anuradha Illendula Elishama Michel Sydney henriques Todd Fox Susan Walker Marya Dunlap-Brown Craig L.Slingluff Jr Mark Kester helena W.Snyde School of Engineering and Applied Sciences University of VirginiaVirginiaVA 22904USA Department of Surgery School of MedicineUniversity of VirginiaVirginiaVA 22903USA Department of Dermatology School of MedicineUniversity of VirginiaVirginiaVA 22903USA Department of Pharmacology School of MedicineUniversity of VirginiaVirginiaVA 22903USA University of Virginia nanoSTAR Institute VirginiaVA 22903USA Molecular Immunologic&Translational Sciences(MITS)Core School of MedicineUniversity of VirginiaVirginiaVA 22903USA Department of Materials Science School of Engineering and Applied SciencesUniversity of VirginiaVirginiaVA 22903USA

Aim:Despite the huge advancements in cancer therapies and treatments over the past decade,most patients with metastasized melanoma still die from the *** poor prognosis largely results from resistance to conventional chemotherapies and other cytotoxic *** have previously identified 6 antigenic peptides derived from melanomas that have proven efficacious for activating CD4+T cells in clinical trials for *** aim was to improve pharmacodynamics,pharmacokinetic and toxicological parameters by individually encapsulating each of the 6 melanoma helper peptides within their own immunogenic ***:We modified these liposomes as necessary to account for differences in the peptides’chemical properties,resulting in 3 distinct *** further enhance immunogenicity,we also incorporated KDO2,a TLR4 agonist,into the lipid bilayer of all nanoliposome *** then conducted in vivo imaging studies in mice and ex vivo cell studies from 2 patient samples who both strongly expressed one of the identified ***:We demonstrate that these liposomes,loaded with the different melanoma helper peptides,can be readily mixed together and simultaneously delivered without toxicity in *** liposomes are capable of being diffused to the secondary lymphoid organs very quickly and for at least 6 *** addition,we show that these immunogenic liposomes enhance immune responses to specific peptides ex ***:Lipid-based delivery systems,including nanoliposomes and lipid nanoparticles,have now been validated for pharmacological(small molecules,bioactive lipids)and molecular(mRNA,siRNA)therapeutic ***,the utility of these formulations as cancer vaccines,delivering antigenic peptides,has not yet achieved the same degree of commercial ***,we describe the novel and successful development of a nanoliposome-based cancer vaccine for *** vaccines help to circumvent drug resistance by increasing a

关键词： Nanoliposomes nanoscale drug delivery cancer vaccines metastasized melanoma peptides melanoma drug resistance

Helicobacter pylori and EBV in gastric carcinomas:Methylation status and microsatellite instability

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World Journal of Gastroenterology 2010年第3期16卷 312-319页

作者： Adriana Camargo Ferrasi Nídia Alice Pinheiro silvia helena Barem Rabenhorst Otávia Luisa Caballero Maria Aparecida Marchesan Rodrigues Fabrício de Carvalho Celso Vieira de Souza Leite Marcia Valéria Pitombeira Ferreira Marcos Aurélio Pessoa Barros Maria Inês de Moura Campos Pardini Blood Transfusion Center Botucatu Medical SchoolUNESP-Sao Paulo State University Department of Pathology-Federal University of Ceara Ludwig Institute of Cancer Research Department of Pathology Medical SchoolUNESP-Sao Paulo State University Department of Gastroenterology Surgery Medical SchoolUNESP-Sao Paulo State University

AIM:To verify the methylation status of CDH1, DAPK, COX2, hMLH1 and CDKN2A genes and to evaluate their association with Helicobacter pylori (H. pylori)-cagA+ and Epstein Barr virus (EBV) infections in gastric ***: Methylation-specific PCR (MSP) assay was performed in 89 primary gastric carcinomas (intestinal and diffuse types). Microsatellite instability (MSI) analysis was performed using the BAT26 primer set and PCR products were analyzed with the ABI PRISM 3100 Genetic Analyzer using Genescan 3.7 software (Applied Biosystems). Detection of H. pylori and genotyping were performed by PCR, using specifi c primers for ureaseC and cagA genes. The presence of EBV was assessed by in situ hybridization. Statistical analyses were performed using the χ2 or Fisher's exact ***: The most frequent hypermethylated gene was COX-2 (63.5%) followed by DAPK (55.7%), CDH1 (51%), CDKN2A (36%) and hMLH1 (30.3%). Intestinal and diffuse adenocarcinomas showed different methylation profiles and there was an association between methylation of E-CDH1 and H. pylori-cagA+ in the intestinal adenocarcinoma type. MSI was correlated with hMLH1 methylation. There was an inverse correlation between DAPK hypermethylation and ***: We found a strong association between CDH1 methylation and H. pylori-cagA+ in intestinal-type gastric cancer, association of MSI and better prognosis and an heterogeneous COX-2 overexpression.

关键词： Gastric cancer Methylation Microsatellite instability Helicobacter pylori Epstein Barr virus

Interrelationship between chromosome 8 aneuploidy,C-MYC amplification and increased expression in individuals from northern Brazil with gastric adenocarcinoma

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World Journal of Gastroenterology 2006年第38期12卷 6207-6211页

作者： Danielle Queiroz Calcagno Mariana Ferreira Leal Aline Damaceno Seabra Andre Salim Khayat Elizabeth Suchi Chen Samia Demachki Paulo Pimentel Assumpcao Mario Henrique Girao Faria silvia helena Barem Rabenhorst Márcia Valéria Pitombeira Ferreira Marília de Arruda Cardoso Smith Rommel Rodríguez Burbano Human Cytogenetics and Toxicological Genetics Laboratory Department of BiologyCenter of Biological SciencesFederal University of ParáBelémPABrazil Genetics Division Department of Morphology Federal University of Sao PauloSao PauloSPBrazil Department of Pathology and Surgery Service Federal University of ParáBelémPABrazil Joao de Barros Barreto University Hospital Federal University of ParáBelémPABrazil Molecular Genetics Laboratory Department of Pathology Medical SchoolFederal University of CearáFortalezaCEBrazil

AIM: To investigate chromosome 8 numerical aberra- tions, C-MYC oncogene alterations and its expression in gastric cancer and to correlate these findings with histo- pathological characteristics of gastric tumors. METHODS: Specimens were collected surgically from seven patients with gastric adenocarcinomas. Immu- nostaining for C-MYC and dual-color fluorescence in situ hybridization (FISH) for C-MYC gene and chromosome 8 centromere were performed. RESULTS: All the cases showed chromosome 8 aneu- ploidy and C-MYC amplification, in both the diffuse and intestinal histopathological types of Lauren. No significant difference (P < 0.05) was observed between the level ofchromosome 8 ploidy and the site, stage or histological type of the adenocarcinomas. C-MYC high amplification, like homogeneously stained regions (HSRs) and double minutes (DMs), was observed only in the intestinal-type. Structural rearrangement of C-MYC, like translocation, was observed only in the diffuse type. Regarding C-MYC gene, a significant difference (P < 0.05) was observed between the two histological types. The C-MYC protein was expressed in all the studied cases. In the intestinal- type the C-MYC immunoreactivity was localized only in the nucleus and in the diffuse type in the nucleus and cytoplasm. CONCLUSION: Distinct patterns of alterations between intestinal and diffuse types of gastric tumors support the hypothesis that these types follow different genetic path- ways.

关键词： Chromosome 8 aneuploidy C-MYC amplification Immunostaining Gastric adenocarcinoma

In vitro and in vivo trypanocidal action of aescin and aescin liposomes against Trypanosoma evansi in experimental mice

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Asian Pacific Journal of Tropical Biomedicine 2014年第12期4卷 947-951页

作者： Matheus Dellamea Baldissera Nathieli Bianchin Bottari Thirssa helena Grando Roberto Christ Vianna Santos Ana Julia Figueiro Dalcin Patrfcia Gomes Renata Platcheck Raffin Carine Eloise Prestes Zimmerman Janio Morais Santurio silvia Gonzalez Monteiro Aleksandro Schafer Da Silva Department of Microbiology and Parasitology Universidade Federal de Santa Maria(UFSM) Laboratory of Microbiology Centro Universitdrio Franciscano Laboratory of Nanotechnology Centro Universitdrio Franciscano Department of Animal Science Universidade do Estado de Santa Catarina(UDESC)

Objective:To verify the trypanocidal effectiveness of aescin and aescin liposomes against Trypanosoma evansi in vitro and in ***:Aescin and aescin liposomes were used in vitro on trypomastigotes at different concentrations(0.5%,1.0%and 2.0%) and exposure times(0,1,3,6 and 9 h).In vivo tests were performed using mice as the experimental *** evansi infected mice were treated with aescin and aescin liposomes with doses of 60 and 100 mg/kg during 4 ***:The three concentrations tested in free form and nanoencapsulated showed trypanocidal activity in vitro,completely eliminating the parasites in small concentration after6 h of *** treated with aescin(100 mg/kg) and aescin liposomes(100 mg/kg)showed increase in longevity,however without curative ***:Active compounds present in natural products,such as aescin,may potentiate the treatment of trypanosomosis when used in association with other trypanocidal drugs.

关键词： Surra Nanotechnology Aesculus hippocastanum Trypanosome