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Defective Kernel 39 encodes a PPR protein required for seed development in maize

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Journal of Integrative Plant Biology 2018年第1期60卷 45-64页

作者： Xiaojie Li Wei Gu silong sun Zongliang Chen Jing Chen Weibin Song Haiming Zhao Jinsheng Lai State Key Laboratory of Agrobiotechnology and National Maize Improvement Center Department of Plant Genetics and Breeding China Agricultural University

RNA editing is a posttranscriptional process that is important in mitochondria and plastids of higher plants. All RNA editing-specific trans-factors reported so far belong to PLS-class of pentatricopeptide repeat(PPR)proteins. Here, we report the map-based cloning and molecular characterization of a defective kernel mutant dek39 in maize. Loss of Dek39 function leads to delayed embryogenesis and endosperm development, reduced kernel size, and seedling lethality. Dek39 encodes an E subclass PPR protein that targets to both mitochondria and chloroplasts, and is involved in RNA editing in mitochondrial NADH dehydrogenase3(nad3) at nad3-247 and nad3-275. C-to-U editing of nad3-275 is not conserved and even lost in Arabidopsis, consistent with the idea that no close DEK39 homologs are present in Arabidopsis. However, the amino acids generated by editing nad3-247 and nad3-275 are highly conserved in many other plant species, and the reductions of editing at these two sites decrease the activity of mitochondria NADH dehydrogenase complex I,indicating that the alteration of amino acid sequence is necessary for Nad3 function. Our results indicate that Dek39 encodes an E sub-class PPR protein that is involved in RNA editing of multiple sites and is necessary for seed development of maize.

关键词： PPR Defective Kernel 39 encodes a PPR protein required for seed development in maize

Experimental design of paraffin/methylated melamine-formaldehyde microencapsulated composite phase change material and the application in battery thermal management system

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Journal of Materials Science & Technology 2024年第2期169卷 124-136页

作者： Que Huang silong Wang Jichun He Dengji Xu Safaa NAbdou Mohamed MIbrahim Shiqi sun Yanjun Chen Handong Li Ben Bin Xu Changcheng Liu Zeinhom M.El-Bahy Zhanhu Guo School of Environment and Safety Engineering North University of ChinaTaiyuan 030051China Institute of Advanced Energy Materials and Systems North University of ChinaTaiyuan 030051China School of Resources and Safety Engineering Central South UniversityChangsha 410010China School of Medical and BioInformation Engineering Northeastern UniversityShenyang 110819China Department of Chemistry Khurmah University CollegeTaif UniversityP.O.Box 11099Taif 21944Saudi Arabia Department of Chemistry College of ScienceTaif UniversityP.O.Box 11099Taif 21944Saudi Arabia Mechanical and Construction Engineering Faculty of Engineering and EnvironmentNorthumbria UniversityNewcastle Upon Tyne NE18STUK College of Materials Science and Engineering Taiyuan University of Science and TechnologyTaiyuan 030024China Department of Chemistry Faculty of ScienceAl-Azhar UniversityNasr City 11884CairoEgypt

In order to maintain the optimal operating temperature of the battery surface and meet the demand for thermal storage technology,battery thermal management system based on phase change materials has attracted increasing *** this work,a kind of core-shell structured microcapsule was synthesized by an in-situ polymerization,where paraffin was used as the core,while methanol was applied to mod-ify the melamine-formaldehyde shell to reduce toxicity and improve thermal ***,three different types of heat conductive fillers with the same content of 10 wt.%,i.e.,nano-Al_(2)O_(3),nano-ZnO and carbon nanotubes were added,generating *** microcapsules were uniform,and were not affected by the thermal fillers,which were evenly dispersed *** composite sample with carbon nanotubes(10 wt.%)showed the highest thermal conductivity of 0.50 W/(m K)and latent heat of 139.64 J/***,according to the leakage testing and battery charge/discharge experiments,compared with Al_(2)O_(3)and ZnO,the addition of carbon nanotubes remarkably enhances the heat storage ability as latent heat from 126.98 J/g for the prepared sample with Al_(2)O_(3)and 125.86 J/g for the one with ZnO,then to 139.64 J/g,as well as dissipation performance as a cooling effect by decreasing the sur-face temperature of battery from 2%to 12%of microcapsule,composite sample with carbon nanotubes presents a broad application prospect in battery thermal management system and energy storage field.

关键词： Microcapsule Phase change material Battery Carbon nanotube Safety

Population genomic analysis and Genome wide association studies through the re-sequencing of 778 maize inbred lines

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Population genomic analysis and Genome wide association stud...

第一届全国玉米生物学学术研讨会

作者：史俊鹏 Zhipeng Liu Baobao Wang Xin Wang Andrew Hauck Shaojun Xie Hainan Zhao Mei Zhang silong sun Haiming Zhao Weibin Song Jinsheng Lai State Key Laboratory of Agrobiotechnology and National Maize Improvement Center China Agricultural University

Modern maize breeding process have generated enormous amount of genetic variants which may contribute to the phenotype *** top of re-sequencing data of 278 maize inbred lines,we expanded the population to 778 lines with.5x depth on average and identified 56 million *** 30%of the SNPs are novel and enriched in rare *** non-syn/syn ratio is 1.09 which is comparable with

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燃料电池氢气供应系统多功能测试平台设计

汽车工程 2024年第7期46卷 1147-1156,1196页

作者：张思龙梁满志孙珩凯陈继成张辉北京航空航天大学交通科学与工程学院北京100084 常熟理工学院汽车工程学院常熟225500

近年来,随着燃料电池功率的不断提升,氢气供给系统朝向带有氢循环的盲端阳极拓扑构型发展。然而,针对氢气供给和循环的测试系统研究明显滞后,尤其在氢气循环泵和引射器等核心部件的性能测试方面。本文研发了多功能燃料电池氢气供应系统... 详细信息

近年来,随着燃料电池功率的不断提升,氢气供给系统朝向带有氢循环的盲端阳极拓扑构型发展。然而,针对氢气供给和循环的测试系统研究明显滞后,尤其在氢气循环泵和引射器等核心部件的性能测试方面。本文研发了多功能燃料电池氢气供应系统测试平台,实现了对不同构型氢气循环方案的氢气供应系统的零部件测试和特性数据采集、离线标定等功能。平台通过模拟真实电堆的压降、氢气消耗及产水产热,消除了测试对真实电堆性能和寿命产生损耗而造成的额外成本。最后,基于该平台对150 kW燃料电池的氢气供应系统进行了阳极压力控制和阳极吹扫控制测试,验证了所开发测试平台能够针对不同负载满足相应测试需求。

关键词：燃料电池氢气循环测试平台

Wheat genomic study for genetic improvement of traits in China

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Science China(Life Sciences) 2022年第9期65卷 1718-1775页

作者： Jun Xiao Bao Liu Yingyin Yao Zifeng Guo Haiyan Jia Lingrang Kong Aimin Zhang Wujun Ma Zhongfu Ni Shengbao Xu Fei Lu Yuannian Jiao Wuyun Yang Xuelei Lin silong sun Zefu Lu Lifeng Gao Guangyao Zhao Shuanghe Cao Qian Chen Kunpu Zhang Mengcheng Wang Meng Wang Zhaorong Hu Weilong Guo Guoqiang Li Xin Ma Junming Li Fangpu Han Xiangdong Fu Zhengqiang Ma Daowen Wang Xueyong Zhang Hong-Qing Ling Guangmin Xia Yiping Tong Zhiyong Liu Zhonghu He Jizeng Jia Kang Chong Key Laboratory of Plant Molecular Physiology Institute of BotanyChinese Academy of SciencesBeijing 100093China Institute of Crop Sciences Chinese Academy of Agricultural SciencesBeijing 100081China The State Key Laboratory of Plant Cell and Chromosome Engineering Institute of Genetics and Developmental BiologyChinese Academy of SciencesBeijing 100101China State Key Laboratory for Agrobiotechnology and Key Laboratory of Crop Heterosis and Utilization(MOE)and Beijing Key Laboratory of Crop Genetic Improvement China Agricultural UniversityBeijing 100193China The Key Laboratory of Plant Development and Environment Adaptation Biology Ministry of EducationSchool of Life ScienceShandong UniversityQingdao 266237China State Key Laboratory of Crop Biology College of AgronomyShandong Agricultural UniversityTai’an 271018China Crop Genomics and Bioinformatics Center and National Key Laboratory of Crop Genetics and Germplasm Enhancement Nanjing Agricultural UniversityNanjing210095China Key Laboratory of Molecular Epigenetics Northeast Normal UniversityChangchun 130024China College of Agronomy State Key Laboratory of Wheat and Maize Crop Scienceand Center for Crop Genome EngineeringHenan Agricultural UniversityZhengzhou 450002China State Key Laboratory of Crop Stress Biology for Arid Areas College of AgronomyNorthwest A&F UniversityYangling 712100China College of Agronomy Qingdao Agricultural UniversityQingdao 266109China Ministry of Education Key Laboratory of Molecular and Cellular Biology Hebei Collaboration Innovation Center for Cell SignalingHebei Key Laboratory of Molecular and Cellular BiologyCollege of Life SciencesHebei Normal UniversityShijiazhuang 050024China Institute of Crop Research Sichuan Academy of Agricultural SciencesChengdu 610066China State Key Laboratory of Systematic and Evolutionary Botany Institute of BotanyChinese Academy of SciencesBeijing 100093China State Key Laboratory of Soil and Sustainable Agriculture Institute of Soil ScienceChinese Academy of Sciences

Bread wheat(Triticum aestivum L.)is a major crop that feeds 40%of the world’s *** the past several decades,advances in genomics have led to tremendous achievements in understanding the origin and domestication of wheat,and the genetic basis of agronomically important traits,which promote the breeding of elite *** this review,we focus on progress that has been made in genomic research and genetic improvement of traits such as grain yield,end-use traits,flowering regulation,nutrient use efficiency,and biotic and abiotic stress responses,and various breeding strategies that contributed mainly by Chinese *** genomic research in wheat is entering a new era with the availability of multiple reference wheat genome assemblies and the development of cutting-edge technologies such as precise genome editing tools,highthroughput phenotyping platforms,sequencing-based cloning strategies,high-efficiency genetic transformation systems,and speed-breeding *** insights will further extend our understanding of the molecular mechanisms and regulatory networks underlying agronomic traits and facilitate the breeding process,ultimately contributing to more sustainable agriculture in China and throughout the world.

关键词： wheat genomics genetic improvement China

基于ARIMA的GDP预测模型的构建及应用

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辽宁科技大学学报 2014年第4期37卷 337-342,349页

作者：孙泗龙李少博范辰刘洪辽宁科技大学理学院辽宁鞍山114051

为了准确预测山东省＂十二五＂期间GDP增长趋势,本文通过分析山东省1978-2012年GDP数据,构建了基于ARIMA模型的GDP预测模型。结果表明,ARIMA（2,2,2）模型能够较好地拟合山东省1978-2012年间GDP变化,在此基础上对山东省2013-2015年GDP... 详细信息

为了准确预测山东省＂十二五＂期间GDP增长趋势,本文通过分析山东省1978-2012年GDP数据,构建了基于ARIMA模型的GDP预测模型。结果表明,ARIMA（2,2,2）模型能够较好地拟合山东省1978-2012年间GDP变化,在此基础上对山东省2013-2015年GDP进行了预测,结果依次为59 613.02,69 204.16,80 328.56亿元。

关键词：差分自回归移动平均模型预测国内生产总值

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天然“转基因”使小麦获得赤霉病抗性

自然杂志 2020年第4期42卷 340-346页

作者：葛文扬孙思龙王宏伟孔令让山东农业大学农学院作物生物学国家重点实验室山东泰安271018

小麦赤霉病被称作小麦"癌症",不仅严重危害小麦的产量和品质,而且会导致脱氧雪腐镰刀菌烯醇(DON)等真菌毒素的严重污染,而挖掘应用抗赤霉病基因培育抗病小麦品种是抵御病原菌侵害最有效的方法之一。本研究基于组装的二倍体长穗偃麦草参... 详细信息

小麦赤霉病被称作小麦"癌症",不仅严重危害小麦的产量和品质,而且会导致脱氧雪腐镰刀菌烯醇(DON)等真菌毒素的严重污染,而挖掘应用抗赤霉病基因培育抗病小麦品种是抵御病原菌侵害最有效的方法之一。本研究基于组装的二倍体长穗偃麦草参考基因组、BAC文库等,利用图位克隆技术克隆了一个主效抗小麦赤霉病基因Fhb7。该基因编码一个具有广谱催化作用的谷胱甘肽S-转移酶,通过去环氧化机制对单端孢霉烯族毒素起到解毒作用。令人惊奇的是,在植物界没有发现Fhb7的同源基因,但多个证据表明二倍体长穗偃麦草早期可能与Epichloe属的内生真菌形成共生体,通过水平基因转移将Epichloe Fhb7的DNA序列整合到长穗偃麦草基因组中,从而进化出抗镰刀菌属病原菌侵染的功能。Fhb7基因导入小麦后,在不同的遗传背景下,不会造成产量的明显损失,同时对小麦赤霉病和茎基腐病具有广谱抗性,因此在小麦抗病育种中具有广阔的应用前景。

关键词：小麦赤霉病 Fhb7 长穗偃麦草水平基因转移

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太阳辐照度测量中孔径光阑衍射效应

光学精密工程 2024年第15期32卷 2363-2373页

作者：余杰玲董航衣小龙林雨辰王思龙苗庆祥孙瑞高展鹏方伟叶新中国科学院长春光学精密机械与物理研究吉林长春130033 中国科学院大学北京100049 探月与航天工程中心北京100048 长春理工大学光电信息科学与工程(理) 吉林长春130013 吉林工程技术师范学院电气与信息工程学院吉林长春130062 哈尔滨工程大学材料科学与化学工程学院黑龙江哈尔滨150006

针对当前在辐照度测量中对孔径光阑衍射效应的评估仍停留在理论计算层面,无法评估衍射修正结果不确定度的现状,研究了光阑衍射效应实验检测方法,以校正孔径光阑的衍射效应并提升太阳总辐照度的测量精度。基于暗场成像技术和夫琅和费远... 详细信息

针对当前在辐照度测量中对孔径光阑衍射效应的评估仍停留在理论计算层面,无法评估衍射修正结果不确定度的现状,研究了光阑衍射效应实验检测方法,以校正孔径光阑的衍射效应并提升太阳总辐照度的测量精度。基于暗场成像技术和夫琅和费远场衍射理论,设计孔径光阑的衍射效应检测方法,开展检测实验,通过研究探测器线性度标定、背景信号校正、衍射图像数据分析等,建立数据修正处理流程,获取孔径光阑在前结构的太阳绝对辐射计衍射效应值,并对实验结果进行不确定度分析。测试结果显示:衍射效应测量值为8.81×10^(-4),合成不确定度为6.49%。研究成果将为我国下一代风云气象卫星太阳辐射监测仪衍射效应的精确校正及实现高精度太阳总辐照度的测量奠定关键技术基础。

关键词：衍射效应孔径光阑衍射角图像校正

Horizontal gene transfer of Fhb7 from a fungus underlies Fusarium head blight resistance in wheat

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Horizontal gene transfer of Fhb7 from a fungus underlies Fus...