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T4-like coliphage ΦKAZ14 virulent to pathogenic and extended spectrum β-lactamase-producing Escherichia coli of poultry origin

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Virologica Sinica 2015年第1期30卷 73-75页

作者： Kaikabo Adamu Ahmad Abdulkarim Sabo Mohanmmed Faridah Abas sieo chin chin Department of Food Science Faculty of Food Science and Technology Universitiy Putra Malaysia Bacterial Research Department National Veterinary Research Institute Department of Microbiology Faculty of Biotechnology and Biomolecular Sciences Universitiy Putra Malaysia

Dear Editor,Bacteriophages(otherwise called phages)are a type of virus that infect *** viral type has found useful applications in the control of bacterial pathogens in foods and food processing *** addition,phages ma... 详细信息

关键词： producing pathogenic lactamase ESBL otherwise environments phage chicken poultry avian

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Cloning of a novel phytase from an anaerobic rumen bacterium, Mitsuokella jalaludinii, and its expression in Escherichia coli

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Journal of Integrative Agriculture 2015年第9期14卷 1816-1826页

作者： TAN Wan-qin Phang Chiun Yee sieo chin chin Yiap Beow chin Clemente Michael Wong Vui Ling Norhani Abdullah Son Radu Ho Yin Wan Laboratory of Vaccines and Immunotherapeutics Institute of BioscienceUniversiti Putra Malaysia Department of Microbiology Faculty of Biotechnology and Biomolecular SciencesUniversiti Putra Malaysia Department of Life Sciences School of Pharmacy and HealthFaculty of Medicine and HealthInternational Medical University Biotechnology Research Institute Universiti Malaysia Sabah Institute of Tropical Agriculture Universiti Putra Malaysia Department of Biochemistry Faculty of Biotechnology and Biomolecular SciencesUniversiti Putra Malaysia Department of Food Science Faculty of Food Science and TechnologyUniversiti Putra Malaysia

The ful length phytase gene of Mitsuokel a jalaludini was successful y cloned and was found to be 1 047 bp in length, with 348 amino acids, and was designated as PHY7 phytase gene. A comparison of the sequence of PHY7 phytase gene of M. jalaludini with various microbial phytase gene sequences showed that it was not similar to those from other bacteria except Selenomonas ruminatium, thus suggesting that they may both express a new class of phytase. The PHY7 phytase gene was subsequently subcloned into bacterial expression vector, pET32a, for expression in Escherichia coli strain Ro-setta-gami. Expression of the recombinant phytase gene was optimised and characterised. The recombinant phytase was estimated to be approximately 55 kDa by SDS-PAGE analysis. The recombinant phytase exhibited optimum activity at 55°C, pH 4.5 and showed good pH stability from pH 3.5 to 5.5 (>78%relative activity). Metal ions such as Ca2+, Mg2+, and K+were found to exert signiifcant stimulatory effect on the recombinant phytase activity while Cu2+, Fe3+, and Zn2+greatly inhibited the enzyme activity. The recombinant phytase showed moderate resistance to trypsin proteolysis, but susceptible to pepsin proteolysis. The results of the study showed that several characteristics of recombinant phytase were slightly different from the native enzyme. Unfavourable characteristics such as reduced pH stability and metal ion effects should be taken into consideration during feed enzyme formulation.

关键词： phytase cloning recombinant Escherichia coli Mitsuokella jalaludinii

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Potentials of <i>Melastoma malabathricum</i>Linn. Flower and Fruit Extracts as Antimicrobial Infusions

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American Journal of Plant Sciences 2012年第8期3卷 1127-1134页

作者： Siti Nurhadis Che Omar Janna Ong Abdullah Khairul Anuar Khairoji sieo chin chin Muhajir Hamid Department of Microbiology Faculty of Biotechnology and Biomolecular Sciences Universiti Putra Malaysia Serdang Malaysia Institute of Bioscience Universiti Putra Malaysia Serdang Malaysia

Melastoma malabathricum Linn. is a shrub that belongs to the family Melastomataceae and a common herbal plant used in folk medicines to treat inflamed wounds. This study was carried out with the aim to evaluate the inhibitory activities of different concentrations of the M. malabathricum Linn. flower and fruit crude extracts against a variety of microorganisms. The inhibitory effects of both extracts were tested against the microorganisms using the disc diffusion method. The lowest concentrations of the extracts producing inhibition zones against the test microorganisms were used to determine their Minimum Inhibitory Concentrations (MICs) and Minimum Microbicidal Concentrations (MMCs). Both crude extracts showed strong inhibitory activities against Gram-positive bacteria. The range of MIC values for the crude flower and fruit extracts on all the bacteria tested were 12.5 to 100.0 mg/ml. Overall, Gram-positive bacteria were more susceptible to the crude extracts compared to Gram-negative species, potentiating a possible use of the extracts to inhibit or kill potential pathogens.

关键词： Melastoma malabathricum Linn. Flower Fruit Disc Diffusion Method Minimal Inhibitory Concentration Minimal Microbicidal Concentration

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