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Acute Oral Toxicity Studies of a Chinese Herbal Preparation Shenrong Bunao Capsule

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Agricultural Biotechnology 2017年第3期6卷 62-63页

作者： Ping LI Xiangling QU sibu ma Ming LIU The Second Affiliated Hospital of Guiyang College of Traditional Chinese Medicine Guiyang College of Traditional Chinese Medicine

[ Objectives] This study was conducted to evaluate the acute oral toxicity of Shenrong Bunao Capsule, and to provide a theoretical basis for drug devel- opmcn! and clinical trims. [ Methods] Kunming mice were randomly assigned into two groups： an experimental group and a control group. In experimental group, Shenrong Bunao Capsule solution at maximum concentration of 45% was administered orally by gavage once at a dose of 30 ml/kg body weight （ BW）, while an equal volume of saline solution was given in the control group. Then, the appearance, behavior, mental state, diet, feces, urine, coat, skin color, respiration, nose, eyes and oral secretions and other daily activities of the mice were observed for 14 d after drug administration. At the end of the experiment, the mice were dissected, and their main organs were examined histopathologically. [Results] The appearance, behavior, respiration, body posture, response to stimuli and weight gain of the mice in control group were all normal. All the mice given 13. 500 g/kg BW Shenrong Bunao Capsule were alive 14 d after drug administration, and their appearance, behavior, respiration, body posture, response to stimuli and weight gains did not show any abnormality. In addition, the body weights of male and female mice had no obvious difference between the experimental group and the control group at the binging, 7 and 14 d after drug administration （P 〈0.05）. [ Conclusions] A maximum dose 13. 500 g/kg BW Shenrong Bunao Capsule, which was equivalent to 141 times of the recommended dosage for adult men was given to the mice, and did not result in any apparent toxic effects, suggesting that the Chines herbal preparation Shenrong Bunao Capsule has very little acute toxicity, and the commonly recommended dosage in clinical trials is safe.

关键词： Shenrong Bunao Capsule Acute toxicity test Median lethal dose maximum dose

Determination of 13 Trace Elements in Anshun Thorn Pear (Rosa roxburghii) and Golden Thorn Pear (Rosa sterilis ***) by ICP-MS

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Agricultural Biotechnology 2019年第2期8卷 157-159,231页

作者： Wei CHEN Kaibin LI Qiuyue KONG sibu ma Wen YANG Anshun Institute for Food and drug control Guiyang College of Traditional Chinese Medicine Minzu University of China

[Objectives] This study was conducted to establish an analytical method for the establishment of 13 trace elements in Anshun thorn pear(Rosa roxburghii) and golden thorn pear(Rosa sterilis S. D. Shi). [Methods] Nitric acid was used as the digestion system for microwave digestion. Inductively coupled plasma mass spectrometry(ICP-MS) was used to determine the trace elements in thorn pear and golden thorn pear. [Results] Be, Ti, V, Cr, Mn, Co, Ni, As, Sr, Mo, Cd, Sb and Hg were determined by ICP-MS method in thorn pear and golden thorn pear. The correlation coefficients of the regression equations of the elements were higher than 0.996; the precision RSD was lower than 2.87%; and the recovery ranged from 94.3% to 109.8%. [Conclusions] The method has the advantages of high sensitivity, good accuracy and simultaneous detection of multiple elements, and provides a reference method and theoretical basis for determining trace elements in thorn pear and golden thorn pear and other fruits.

关键词： Inductively coupled plasma mass spectrometry Microwave digestion method Rosa roxburghii Rosa sterilis S. D. Shi Element analysis

Study on the Mechanism of Pseudostellariae Radix in Regulating Angiogenesis Based on Network Pharmacology and a Dual-screening System

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Agricultural Biotechnology 2023年第5期12卷 98-103,113页

作者： Na LI Yihui CHAI Yuqi YANG Xiang PU Guo FENG sibu ma Lailai LI Guizhou University of Traditional Chinese Medicine Guiyang 550025China

[Objectives]This study was conducted to clarify the action mechanism of Pseudostellariae Radix in regulating angiogenesis by using network pharmacology and a dual-screening system,and to provide a basis for its clinical treatment of cardiovascular diseases.[Methods]The TCMSP database was used for preliminary screening to obtain the active compounds of Pseudostellariae Radix and the protein targets of its *** and OMIM databases were used to search for targets related to *** 3.9.1 was used to construct a drug-target network and protein interaction network of Pseudostellariae Radix in *** GO enrichment analysis and KEGG pathway analysis of the targets of Pseudostellariae Radix in angiogenesis were carried out on Metascape *** effects of the screened active compounds were verified using a dual-screening system.[Results]Six active components of Pseudostellariae Radix,luteolin,acetin,beta-sitosterol,linarin,schottenol and 1-monolinolein,were screened by TCMSP database;and the six active components were predicted with 78 common target proteins related to angiogenesis,of which 19 were core *** Radix mainly intervened in angiogenesis through domain specific binding,ubiquitin-like protein ligase binding,kinase binding and other molecular functions to regulate biological processes such as membrane microdomain,plasma membrane raft and *** results of KEGG enrichment indicated that pathways in cancer,lipid and atherosclerosis,hepatitis B,apoptosis,toxoplasmosis and other key pathways might be the mechanism for the intervention of *** results of the dual-screening system showed that luteolin,acacetin,beta-sitosterol and linarin protected HUVECs and promoted zebrafish angiogenesis.[Conclusions]This study preliminarily demonstrated that luteolin,acacetin,beta-sitosterol and linarin could intervene in angiogenesis through multiple targets and multiple pathways,providing ideas and a scienti

关键词： Pseudostellariae Radix Angiogenesis Network pharmacology Dual-screening system

Determination of 2 Flavonoids in Psidium guajava Linn. from Different Habitats in Guizhou

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Agricultural Biotechnology 2017年第3期6卷 71-74页

作者： Kaibin LI sibu ma Yonghua HE Zonghua ZHENG Wen YANG Chen YAN Anshun Institute for Food and Drug Control Guiyang College of Traditional Chinese Medicine Anshun Hospital of Traditional Chinese Medicine Anshun City People's Hospital

[ Objective] This study was conducted to determinate the contents of hyperoside and isoquercitrin in the leaves of Psidium guajava Linn. from different areas of Guizhou Province, which would provide data support for production and quality control of P. guajava leaf material. [ Method] The contents of hypereside and isoquercitrin were measured by HPLC under following conditions： Agilent XDB-C18,4.6 mm x 250 ram, 5 ttm, CH3CN-0.1% HCOOH （13： 87） as mobile phase, column temporature at 25 ℃, detection wavelength of 257 mn and flow rate at 1.0 ml/min. [ Result ] The results showed that leaves of P. guajava from dif- ferent producing areas contained variable hypereaide and isoquercitrin contents in the same period, and the differences in quality were obvious between different are- as. [ Conclusion] The method for detecting the contents of hypereside and isoquercitrin in P. guajava leaf materials is simple with reliable accurate results. The P. guajava leaf material produced in Gouting Township, Luedian County has better quality.

关键词： Leaves of Psidium guajava Linn. Hyperoside Isoquercitrin

Study on Mechanism of Dendrobium officinale Kimura et Migo in Preventing and Treating Exercise-induced Muscle Damage(EIMD)in Rats

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Agricultural Biotechnology 2023年第6期12卷 82-87页

作者： Peihong CAI Jiangyan XIONG Zelin ZHAO sibu ma Yuanyu LONG Danguo WEN Litao XIA Qinmei ZHU Guizhou University of Traditional Chinese Medicine Guiyang 550025China

[Objectives]This study was conducted to observe the mechanism of Dendrobium officinale Kimura et Migo on gastrocnemius muscle in rats with exercise-induced muscle damage(EIMD).[Methods]The micro-injury model of skeletal muscle was established by treadmill *** two SD rats were randomly divided into a control group,1,12 and 24 h exercise groups,*** 2 ml+1 h exercise group,*** 2 ml+12 h exercise group,and *** 2 ml+24 h exercise group,with 6 rats in each *** *** groups were given the drug once in the morning and once in the evening at a dose of 2 ml/time,a week in *** for the quiet group,the samples were collected from the 1,12 and 24 h exercise groups after anesthesia following 1,12 and 24 h of exercise for the last time,respectively,and the *** 2 ml+1 h exercise group,*** 2 ml+12 h exercise group and *** 2 ml+24 h exercise group were also sampled after anesthesia following 1,12 and 24 h of exercise for the last time,*** contents of ATP,CK-MM and CK in rat serum were determined by enzyme-linked immunosorbent assay(ELISA).The histopathological changes of gastrocnemius muscle were observed by HE *** and Western-blot detection were carried out to analyze the effects of *** on IGF-1 mRNA and protein expression in gastrocnemius muscle.[Results]Compared with the quiet group,the ATP contents in the serum of rats in the 1,12 and 24 h exercise groups significantly decreased(P<0.01),while the CK and CK-MM contents significantly increased(P<0.01).The expression of IGF-1 mRNA and protein in the gastrocnemius muscle tissue significantly increased(P<0.01).Compared with the 1 h exercise group,the ATP content and IGF-1 protein expression in the gastrocnemius muscle tissue of the *** liquid+1 h exercise group significantly increased(P<0.05),while the CK and CK-MM contents significantly decreased(P<0.01).Compared with the 12 h exercise group,the ***

关键词： Dendrobium officinale Kimura et Migo Exercise-induced muscle damage Gastrocnemius muscle IGF-1 mRNA

Therapeutic Effect and Mechanism of Jipei Dilong Ointment on Acute Soft Tissue Injury in Rats

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Agricultural Biotechnology 2023年第5期12卷 35-42页

作者： Yihui CHAI Haotian WANG Shiyun YE Lailai LI Baoying HUA Jinghua RUAN Xiang PU Liyan ZHANG sibu ma Guizhou University of Traditional Chinese Medicine Guiyang 550025China First Affiliated Hospital of Guizhou University of Traditional Chinese Medicine Guiyang 550001China

[Objectives]This study was conducted to observe the therapeutic effect of Jipei Dilong Ointment on rats with acute soft tissue injury caused by heavy objects and to explore its action mechanism.[Methods]Thirty six rats were randomly divided into six groups(control group,model group,high-dose Jipei Dilong Ointment group(JP-H),medium-dose Jipei Dilong Ointment group(JP-M),low-dose Jipei Dilong Ointment group(JP-L)and diclofenac group).Except for the Control group,other groups were subjected to modeling of acute soft tissue injury by the weight impact *** administration was performed once a day for nine consecutive *** local appearance score and activity disorder score were determined after soft tissue injury in *** staining was used to detect the pathological changes of injured soft tissues in ***-PCR was used to detect the relative mRNA expressions of Bax,Bcl-2,MMP-9 and TIMP-1 in injured soft tissues of *** Blot was used to detect the protein expressions of MMP-9,TIMP-1,TLR4,MyD88 and NF-κB p65 in injured soft tissues of *** were statistically analyzed.[Results]Compared with the model group,Jipei Dilong Ointment could significantly improve the appearance symptoms such as swelling and ecchymosis in the injured area and the movement function of the affected limb(Pmatory cells and widening of the intermuscular space caused by *** them,the JP-H group and the diclofenac group had more significant curative *** 9 d of administration,each administration group could significantly up-regulate the ratio of Bcl-2/Bax mRNA expression level(P0.05).The expression level of NF-κB p65 protein in each administration group was significantly decreased(P<0.01).The protein expression levels of TLR4 and MyD88 and the ratio of MMP-9/TIMP-1 protein expression level in each administration

关键词： Jipei Dilong Ointment Acute soft tissue injury NF-κB p65 TLR4 MyD88

Effects of Astragalus membranaceus on Energy Metabolism and Expression of CNTF Protein in Skeletal Muscle of Exercise-induced Fatigue Rats

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Agricultural Biotechnology 2023年第6期12卷 19-24,29页

作者： Yueqi ZHOU Xue ZHANG Zelin ZHAO Yuanxia SHEN Li YANG Song WANG Junying TIAN sibu ma Shiyan HUANG Guizhou University of Traditional Chinese Medicine Guiyang 550025China

[Objectives]This study was conducted to investigate the effects of Astragalus membranaceus in different groups on energy metabolism and CNTF protein expression in skeletal muscle of exercise-induced fatigue rats.[Methods]Thirty-five clean male SD rats were randomly divided into a normal group,and low-,meddle-and high-dose groups of *** aqueous solution,with 7 rats in each *** low-dose,medium-dose and high-dose groups were given by gavage at 0.65,1.3 and 2.6 g/kg,respectively,while the normal group and the model group were given normal food and *** weight of rats was *** contents of serum urea,lactate,muscle glycogen,liver glycogen and CNTF expression were detected.[Results]After modeling,compared with the normal group,the serum lactate and urea contents of rats in the model group significantly increased(P<0.01),while the muscle glycogen content(P<0.01)and liver glycogen content(P<0.05)of the skeletal muscle significantly *** with the model group,the low-,meddle-and high-dose groups of *** significantly reduced the levels of lactate and urea in serum(P<0.01),while the levels of muscle glycogen and liver glycogen in the skeletal muscle significantly increased(P<0.01,P<0.05).[Conclusions]This study provides a good research foundation for the treatment of exercise-induced fatigue using traditional Chinese herb *** in modern clinical practice.

关键词： Astragalus membranaceus Exercise fatigue Energy metabolism Skeletal muscle Expression of CNTF protein

Protective Effect and Molecular Mechanism of Shui People's Classic Prescription Jipei Dilong Ointment on Osteoarthritis in Rats

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Medicinal Plant 2023年第5期14卷 57-64页

作者： Lailai LI Baoying HUA Shiyun YE Yihui CHAI Haotian WANG Jinghua RUAN Xiang PU Liyan ZHANG sibu ma Guizhou University of Traditional Chinese Medicine Guiyang 550025China The First Affiliated Hospital of Guizhou University of Traditional Chinese Medicine Guiyang 550001China

[Objectives]To explore the protective effect and molecular mechanism of Shui People s Classic Prescription Jipei Dilong Ointment on knee osteoarthritis in rats.[Methods]72 SPF male SD rats were divided into control group,model group,Jipei Dilong Ointment high,medium and low dose groups,and positive drug Diclofenac group,with 12 rats in each *** the control group,all other groups were injected with 0.2 mL of 2%papain and 0.3%L-cysteine mixture into knee joint cavity to establish osteoarthritis model,while the control group was injected with the same amount of normal *** Lequesne MG score was used to determine the success of the *** successful modeling,external administration was given for 4 *** histopathological changes in articular cartilage and synovium were observed by HE staining;the levels of TNF-α,IL-1βand COX-2 in serum were detected by ELISA,and the relative expression of MMP-9 and TIMP-1 mRNA in cartilage was detected by qRT-PCR;the relative expression of MMP-9,TIMP-1,TLR4,MyD88 and NF-κB in rat cartilage was detected by Western-blot.[Results]Compared with the model group,Jipei Dilong Ointment could significantly reduce the Lequesne MG score and mankin s score of arthritic rats(Pma,necrosis,inflammatory cell infiltration and fibrous tissue proliferation;reduce the expression of MMP-9 mRNA(Pmay be related to the regulation of TLR4/MyD88/NF-κB signaling pathway and the ratio of MMP-9/TIMP-1.

关键词： Jipei Dilong Ointment Osteoarthritis(OA) Traditional Chinese medicine application

Photoshop软件在清风藤类药材鉴定中的应用研究

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中国现代应用药学 2021年第12期38卷 1426-1430页

作者：马四补温迪黄园孙庆文贵州中医药大学贵阳550025

目的应用Photoshop CS3软件处理清风藤属药用植物及其混伪品茎横切面的图像信息,结合统计学分析的方法鉴别该类药材,以此探究应用此方法鉴定茎木类药材的可行性及其鉴定学意义。方法观察分析6种清风藤属药用植物及其混伪品茎横切面特征... 详细信息

目的应用Photoshop CS3软件处理清风藤属药用植物及其混伪品茎横切面的图像信息,结合统计学分析的方法鉴别该类药材,以此探究应用此方法鉴定茎木类药材的可行性及其鉴定学意义。方法观察分析6种清风藤属药用植物及其混伪品茎横切面特征,应用数码摄影,Photoshop CS3软件和SPSS 22.0软件对茎横切面特征进行拍摄、测量和统计分析。结果所观察的6种植物茎横切面中髓/木部比值、髓射线数目等数值存在一定差异。结论采用Photoshop软件处理图像与统计学相结合的方法,可作为清风藤属植物分类及其药材性状鉴别新的参考依据。

关键词：清风藤属 Photoshop 性状鉴定