Study on the Mechanism of Pseudostellariae Radix in Regulating Angiogenesis Based on Network Pharmacology and a Dual-screening System
作者机构：Guizhou University of Traditional Chinese MedicineGuiyang 550025China
出 版 物：《Agricultural Biotechnology》 (农业生物技术（英文版）)
年 卷 期：2023年第12卷第5期
页 面：98-103,113页
学科分类：1008[医学-中药学(可授医学、理学学位)] 1006[医学-中西医结合] 100602[医学-中西医结合临床] 10[医学]
基 金：Supported by Project of Science and Technology Department of Guizhou Province(ZK-546) Project of Science and Technology Department of Guizhou Province(1401) Guizhou Administration of Traditional Chinese Medicine(QZYY-2021-03) Guizhou Provincial Health Commission(gzwkj2021-464)
主 题：Pseudostellariae Radix Angiogenesis Network pharmacology Dual-screening system
摘 要：[Objectives]This study was conducted to clarify the action mechanism of Pseudostellariae Radix in regulating angiogenesis by using network pharmacology and a dual-screening system,and to provide a basis for its clinical treatment of cardiovascular diseases.[Methods]The TCMSP database was used for preliminary screening to obtain the active compounds of Pseudostellariae Radix and the protein targets of its *** and OMIM databases were used to search for targets related to *** 3.9.1 was used to construct a drug-target network and protein interaction network of Pseudostellariae Radix in *** GO enrichment analysis and KEGG pathway analysis of the targets of Pseudostellariae Radix in angiogenesis were carried out on Metascape *** effects of the screened active compounds were verified using a dual-screening system.[Results]Six active components of Pseudostellariae Radix,luteolin,acetin,beta-sitosterol,linarin,schottenol and 1-monolinolein,were screened by TCMSP database;and the six active components were predicted with 78 common target proteins related to angiogenesis,of which 19 were core *** Radix mainly intervened in angiogenesis through domain specific binding,ubiquitin-like protein ligase binding,kinase binding and other molecular functions to regulate biological processes such as membrane microdomain,plasma membrane raft and *** results of KEGG enrichment indicated that pathways in cancer,lipid and atherosclerosis,hepatitis B,apoptosis,toxoplasmosis and other key pathways might be the mechanism for the intervention of *** results of the dual-screening system showed that luteolin,acacetin,beta-sitosterol and linarin protected HUVECs and promoted zebrafish angiogenesis.[Conclusions]This study preliminarily demonstrated that luteolin,acacetin,beta-sitosterol and linarin could intervene in angiogenesis through multiple targets and multiple pathways,providing ideas and a scienti
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