检索结果-南通市图书馆

A herbal pair of Scutellaria barbata D. Don and Scleromitrion diffusum(Willd.) R.J. Wang induced ferroptosis in ovarian cancer A2780 cells via inducing heme catabolism and ferritinophagy

引用

Journal of Integrative Medicine 2024年第6期22卷 665-682页

作者： Zhen Wang Min liu Guang-xing li liu Zhang Kai-yue Ding si-qi li Bing-qing Gao Peng Chen Hyok-Chol Choe Lun-yue Xia Yu-tong Yang Yi liu Xue Sui Jun-nan Ma lin Zhang Institute (College) of Integrative Medicine Dalian Medical University Department of General Surgery the First Affiliated Hospital of Dalian Medical University Advanced Institute of Medical Sciences Dalian Medical University Department of Clinical Medicine Sinuiju Medical University

Objective: Despite the combination of Scutellaria barbata D. Don and Scleromitrion diffusum(Willd.) ***(SB-SD) being a recognized Chinese medicinal herbal pair that is commonly used in the treatment of ovarian cancer, there is a poor understanding of their pharmacological mechanisms. This study examines the antitumor properties and potential mechanisms of SB-SD on human ovarian cancer A2780 cells through a multi-omics approach, establishing a pharmacological basis for clinical ***: A range of mass ratios and reagents were used in the hot reflux extraction of SB-SD. The inhibitory effect of the SB-SD extracts on A2780 cell proliferation was assessed using the cell-counting kit 8assay. A zebrafish tumor implantation model was used to evaluate the effects of SB-SD extracts on tumor growth and metastasis in vivo. Transcriptomics and proteomics were used to investigate alterations in biological pathways in A2780 cells after treatment with different concentrations of SB-SD extract. Cell cycle,cell apoptosis, intracellular free iron concentration, intracellular reactive oxygen species(ROS) concentration, malondialdehyde(MDA), and mitochondrial membrane potential were measured. Real-time quantitative reverse transcription polymerase chain reaction and Western blotting were utilized to investigate the effects of heme catabolism and ferritinophagy on ferroptosis induced by SB-SD extract in A2780 ***: The 70% ethanol extract of SB-SD(a mass ratio of 4:1) inhibited A2780 cell proliferation significantly with a half maximal inhibitory concentration of 660 μg/m L in a concentration-and timedependent manner. Moreover, it effectively suppressed tumor growth and metastasis in a zebrafish tumor implantation model. SB-SD extract induced the accumulation of free iron, ROS, MDA, and mitochondrial damage in A2780 cells. The mechanisms might involve the upregulated expression of ferritinophagyrelated genes microtubule-associated protein 1 light chain 3, auto

关键词： Ferritinophagy Ferroptosis Heme catabolism Ovarian cancer Proteomic Scleromitrion diffusum (Willd.) R.J. Wang Scutellaria barbata D. Don Transcriptomic

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Etiology and prevalence of abnormal serum alanine aminotransferase levels in a general population in Northeast China

引用

Chinese Medical Journal 2011年第17期124卷 2661-2668页

作者： ZHANG Hong JIANG Yan-fang HE Shu-mei SUN Jie GU qing FENG Xiang-wei DU Bing WANG Wei SHI Xiao-dong WANG Chun-yan ZHANG si-qi li Wan-yu NIU Jun-qi Department of Hepatology First Hospital Jilin University Changchun Jilin 130021 China

Background Chronic liver diseases are a major burden in China. Alanine aminotransferase （ALT） can be used as an indicator of hepatocyte damage. In this study, we determined the prevalence and etiologies of elevated ALT in an adult population in Jilin, China. Methods A total of 4072 individuals aged between 18 and 79 years were first interviewed, and then underwent ultrasonography and blood tests. Results The prevalence of elevated ALT was 17.53%. The most noticeable risk factor for ALT elevation was non-alcoholic fatty liver disease （NAFLD）（accounting for 10.79%）, metabolic syndrome （16.25%）, or both （20.31%）. The development of NAFLD occured mostly in female peasants and small businessmen with increased income, age, fasting plasma glucose, body mass index, triglyceridemia, and low-density lipoprotein and decreased education level, high-density lipoprotein. Elevated ALT frequently occured in low education level, male peasants and small businessmen with increased income, body mass index and＇ triglyceride who had NAFLD and/or metabolic syndrome. However, elevated ALT with infection of hepatitis B or C virus was not associated with metabolic disorders, but rather with gender, occupation and increased age. Conclusion The results from the current study demonstrate that elevated ALT is fairly high in the Northeast population （17.53%） and that the cause of its elevation is mostly due to NAFLD and metabolic syndrome.

关键词： alanine aminotransferase hepatitis B virus hepatitis C virus non-alcoholic fatty liver disease

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Dynamic imaging of RNA in living cells by CRISPR-Cas13 systems

Dynamic imaging of RNA in living cells by CRISPR-Cas13 syste...

引用

中国生物化学与分子生物学会2019年全国学术会议暨学会成立四十周年

作者： liang-Zhong Yang Yang Wang si-qi li Run-Wen Yao Huang Wu Gordon G.Carmichael ling-ling Chen State Key Laboratory of Molecular Biology Shanghai Key Laboratory of Molecular Andrology CAS Center for Excellence in Molecular Cell Science Shanghai Institute of Biochemistry and Cell Biology University of Chinese Academy of Sciences Chinese Academy of Sciences Department of Genetics and Developmental Biology University of Connecticut Stem Cell Institute University of Connecticut Health Center School of life Science and Technology ShanghaiTech University

Visualizing the location and dynamics of RNAs, particularly long noncoding RNAs(lncRNAs), in live cells is key to understanding function, but currently available tools are limited. Here, we identify two endonuclease-deficient, singlecomponent programmable RNA guided RNA-targeting Cas13 RNases(dCas13) that allow efficient lncRNA labeling in live cells, even using single guide RNAs with spacers of 20-27 nt in length. Compared to traditional aptamer-based strategies(such as the MS2-MCP system), the optimized dCas13 RNA labeling system achieves a higher labeling efficiency and does not require genetic manipulation. Combination of two different CRISPR-dCas13 systems or CRISPR-dCas13 and MS2-MCP allows dual-color RNA tracking in single live cells and the study of the dynamics of the lncRNA NEAT1-associated paraspeckle nuclear bodies. Collectively, these results demonstrate that the CRISPR-dCas13 RNA labeling system is a userfriendly and efficient tool to visualize RNAs in live cells.

关键词： CRISPR-Cas13 NEAT1 nuclear stress bodies paraspeckle dynamics RNA microscopy RNA dynamics SatⅢ

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Multilevel analysis of the central-peripheral-target organ pathway:contributing to recovery after peripheral nerve injury

引用

Neural Regeneration Research 2025年第10期20卷 2807-2822页

作者： Xizi Song Ruixin li Xiaolei Chu qi li Ruihua li qingwen li Kai-Yu Tong Xiaosong Gu Dong Ming Academy of Medical Engineering and Translational Medicine Tianjin UniversityTianjinChina Haihe Laboratory of Brain-Machine Interface and Human-Machine Fusion TianjinChina Department of Rehabilitation Tianjin University Tianjin HospitalTianjinChina Department of Hand Microsurgery Tianjin University Tianjin HospitalTianjinChina School of Exercise and Health Tianjin University of SportTianjinChina Department of Biomedical Engineering the Chinese University of Hong KongHong Kong Special Administrative RegionChina

Peripheral nerve injury is a common neurological condition that often leads to severe functional limitations and *** on the pathogenesis of peripheral nerve injury has focused on pathological changes at individual injury sites,neglecting multilevel pathological analysis of the overall nervous system and target *** has led to restrictions on current therapeutic *** this paper,we first summarize the potential mechanisms of peripheral nerve injury from a holistic perspective,covering the central nervous system,peripheral nervous system,and target *** peripheral nerve injury,the cortical plasticity of the brain is altered due to damage to and regeneration of peripheral nerves;changes such as neuronal apoptosis and axonal demyelination occur in the spinal *** nerve will undergo axonal regeneration,activation of Schwann cells,inflammatory response,and vascular system regeneration at the injury *** damage to target organs can occur,including skeletal muscle atrophy and sensory receptor *** then provide a brief review of the research advances in therapeutic approaches to peripheral nerve *** main current treatments are conducted passively and include physical factor rehabilitation,pharmacological treatments,cell-based therapies,and physical ***,most treatments only partially address the problem and cannot complete the systematic recovery of the entire central nervous system-peripheral nervous system-target organ ***,we should further explore multilevel treatment options that produce effective,long-lasting results,perhaps requiring a combination of passive(traditional)and active(novel)treatment methods to stimulate rehabilitation at the central-peripheral-target organ levels to achieve better functional recovery.

关键词： central nervous system central peripheral target organ multilevel pathological analysis nerve regeneration peripheral nerve injury peripheral nervous system target organs therapeutic approach

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

参莲提取物对LPS诱导的巨噬细胞炎症反应的影响

引用

中国实验方剂学杂志 2017年第10期23卷 85-91页

作者：刘思思李琦孙立东李玉洁杨庆朱晓新首都医科大学中医药学院北京100069 中国中医科学院中药研究所北京100700 安徽中医药大学合肥230031

目的:探讨参莲(SL)提取物在炎症调节中发挥的治疗性作用及其机制。方法:分别以小鼠巨噬细胞Raw264.7和小鼠腹腔巨噬细胞为实验对象,利用脂多糖(LPS)诱导炎症模型,SL提取物低、中、高剂量(5,10,20 mg·L^(-1))药物处理24 h,另设空白组,... 详细信息

目的:探讨参莲(SL)提取物在炎症调节中发挥的治疗性作用及其机制。方法:分别以小鼠巨噬细胞Raw264.7和小鼠腹腔巨噬细胞为实验对象,利用脂多糖(LPS)诱导炎症模型,SL提取物低、中、高剂量(5,10,20 mg·L^(-1))药物处理24 h,另设空白组,使用酶联免疫吸附测定(EliSA)法及实时荧光定量PCR(Real-time PCR)检测小鼠Raw264.7及腹腔巨噬细胞肿瘤坏死因子-α(TNF-α),白细胞介素-1β(IL-1β)含量及mRNA表达;使用蛋白质免疫印迹法(Western blot)对小鼠Raw264.7核转录因子-κB(NF-κB)信号通路中的p65及磷酸化p65(p-p65)蛋白表达情况进行检测,采用免疫荧光法对小鼠Raw264.7及腹腔巨噬细胞NF-κB p65分子进行亚细胞定位观察SL提取物是否能够影响其入核。结果:与空白组比较,模型组小鼠Raw264.7及腹腔巨噬细胞TNF-α,IL-1β含量及mRNA表达均显著升高,小鼠Raw264.7 p-p65/p65蛋白表达显著升高(P<0.01),免疫荧光观察显示模型组Raw264.7及腹腔巨噬细胞均可见明显入核行为;与模型组比较,SL提取物低、中、高剂量组明显降低小鼠Raw264.7及腹腔巨噬细胞TNF-α,IL-1β含量及mRNA表达(P<0.05,P<0.01),显著降低小鼠Raw264.7及p-p65蛋白(P<0.01),SL提取物中剂量组可明显减弱Raw264.7及腹腔巨噬细胞入核行为。结论:SL提取物能够抑制巨噬细胞的炎症反应,其机制可能与影响巨噬细胞炎性因子的分泌及NF-κB信号通路有关。

关键词：参莲提取物核转录因子-κB(NF-κB) 炎症巨噬细胞

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

黑龙江省黑河地区森林地上生物量和NPP估测及时空格局

引用

应用生态学报 2016年第10期27卷 3070-3080页

作者：赵颖慧李思琪甄贞李凤日魏庆彬东北林业大学林学院哈尔滨150040 黑龙江省环境监测中心站哈尔滨150056

根据黑龙江省黑河地区2005和2010年1390块固定样地数据和东北地区树种生物量异速模型,估算各样地单位面积森林地上生物量(AGB)和净初级生产力(NPP).结合该地区2005和2010年两期ETM+遥感图像,运用地统计克里格与协同克里格法对AGB和NPP... 详细信息

根据黑龙江省黑河地区2005和2010年1390块固定样地数据和东北地区树种生物量异速模型,估算各样地单位面积森林地上生物量(AGB)和净初级生产力(NPP).结合该地区2005和2010年两期ETM+遥感图像,运用地统计克里格与协同克里格法对AGB和NPP进行插值,比较多种方差函数的拟合效果,并用最佳插值方法得出该地区AGB和NPP的分布图;通过两期分布图的对比,分析该区域AGB和NPP随时间和空间的动态变化趋势,及其与地形因子(坡向、坡度、海拔)和不同林分类型的时空变化规律.结果表明:2005—2010年,黑河地区AGB呈现增加趋势,高于40 t·hm^(-2)的生物量面积明显增加;NPP有所下降,出现高NPP地区向低NPP地区转变的现象.AGB和NPP与各地形因子均有一定的相关性,其中,与海拔的相关性明显,说明AGB和NPP的分布受海拔影响显著.研究期间,AGB在各个坡向均有所提高,NPP则降低;AGB和NPP随着坡度增大、海拔增高有增加趋势;常绿针叶林AGB和NPP增长最多,针阔混交林AGB增长最少,落叶阔叶林NPP增长最少.

关键词：地上生物量净初级生产力空间格局

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Metal nanoparticles decorated CoFe-(oxy)hydroxysulfides nanosheets fabricated by a general strategy for electrocatalytic water splitting

引用

Journal of Energy Chemistry 2025年第1期100卷 26-38页

作者： Xiaodong Yang Haochen Shen Xiaoming Xiao Zhichao li qi Zhou Wei Yang Bin Jiang Yongli Sun Luhong Zhang Zhenhua Yan School of Chemical Engineering and Technology Tianjin University Key Laboratory of Advanced Energy Materials Chemistry (Ministry of Education) Nankai University

This study presents a novel method to fabricate metal-decorated,sulfur-doped layered double hydroxides （M/SLDH） through spontaneous redox and sulfurization *** developed Ag/SLDH and Pt/SLDH catalysts with abundant heterogeneous interfaces and hierarchical nanostructures demonstrated outstanding oxygen evolution reaction （OER） and hydrogen evolution reaction （HER） performance,achieving low overpotentials of 212 and 35 mV at 10 mA cm-2in 1 M KOH,*** both anode and cathode in water splitting,they required only 1.47 V to reach 10 mA cm-2and exhibited high structural robustness,maintaining stability at 1000 mA cm-2for 300 ***-situ Raman analysis revealed that the synergistic effects of metal nanoparticles and S doping significantly promote the transformation into the S-Co1-xFexOOH layer,which serves as the active phase for water ***,ultraviolet photoelectron spectroscopy （UPS） and density functional theory （DFT） analyses indicated that incorporating metal nanoparticles and S doping increase electron density near the Fermi level and reduce reaction energy barriers,thus enhancing intrinsic OER and HER *** study provides a scalable strategy for synthesizing high-performance electrocatalysts for water splitting,with promising potential for broader applications.

关键词： LDH Spontaneous reaction Metal nanoparticles Water splitting

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

粒子群算法在GMR-13G系统EVM求解中的应用研究

引用

通信技术 2017年第1期50卷 13-17页

作者：吕宗琦司伟立重庆邮电大学通信与信息工程学院重庆400065 北京中科晶上科技有限公司北京100190

针对GMR-1 3G中EVM(Error Vector Magnitude)算法在π/4-CQPSK调制后产生了误差参数关联性高且求解准确性低的问题,提出了应用粒子群的EVM算法。该算法通过粒子群算法同时估计多个参数,利用误差估计结果修正接收信号,从而得到参考信号,... 详细信息

针对GMR-1 3G中EVM(Error Vector Magnitude)算法在π/4-CQPSK调制后产生了误差参数关联性高且求解准确性低的问题,提出了应用粒子群的EVM算法。该算法通过粒子群算法同时估计多个参数,利用误差估计结果修正接收信号,从而得到参考信号,进而计算EVM结果。仿真结果表明,参考信号近似理想信号,该算法与R&S频谱分析仪FQS26相比,EVM差值为0.31%,EVM不确定度为0.022%。可见,该EVM算法可以应用于GMR-1 3G系统,并能够精确测量EVM。

关键词：粒子群 GMR-13G EVM 调制精度

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

一种基于飞秒激光白内障手术的碎核轨迹设计

引用

激光与红外 2017年第6期47卷 680-685页

作者：张来峰刘思奇范立成盛小明苏州大学机电工程学院江苏苏州215006

针对在飞秒激光辅助晶状体碎核中,需要根据白内障患者的晶状体浑浊等级选择不同的扫描方案,且其每一层的扫描轨迹不连续的问题,提出了一种参数化的螺旋形激光扫描碎核轨迹。对螺旋形扫描轨迹进行了整体参数化定义,解出了扫描轨迹的数学... 详细信息

针对在飞秒激光辅助晶状体碎核中,需要根据白内障患者的晶状体浑浊等级选择不同的扫描方案,且其每一层的扫描轨迹不连续的问题,提出了一种参数化的螺旋形激光扫描碎核轨迹。对螺旋形扫描轨迹进行了整体参数化定义,解出了扫描轨迹的数学模型,并求解了轨迹中关键数据点的坐标值。最后对扫描轨迹数学模型建立了仿真,其仿真结果验证了所求解扫描轨迹数学模型的正确性。此种碎核方案的每一层轨迹连续,可通过改变相应参数实现不同的切割效果,为飞秒激光辅助白内障手术提供了一种新型的碎核方案,具有一定的实用价值。

关键词：飞秒激光激光碎核螺旋轨迹数学模型

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

探析“主客浑受”的理论意义与临床启发

引用

中医药学报 2017年第4期45卷 6-8页

作者：刘思琦李廷利黑龙江中医药大学黑龙江哈尔滨150040

薛氏"主客浑受"理论的形成主要来源于吴氏"主客交"理论的启发。"主客浑受"的"主"指人体营血,"客"指湿热之邪,"主客浑受"乃指湿热郁留于血脉,交浑不解,从而形成一种脉络凝瘀、气血呆滞的病理状态。治疗方法当行气活血,化瘀通络。治疗方... 详细信息

薛氏"主客浑受"理论的形成主要来源于吴氏"主客交"理论的启发。"主客浑受"的"主"指人体营血,"客"指湿热之邪,"主客浑受"乃指湿热郁留于血脉,交浑不解,从而形成一种脉络凝瘀、气血呆滞的病理状态。治疗方法当行气活血,化瘀通络。治疗方药为三甲散加减。将"主客浑受"的理论应用于肝纤维化、慢性萎缩性胃炎以及特发性膜性肾病等疾病的临床治疗,取得了较满意的疗效,提供了新的临床治疗思路。

关键词：主客浑受主客交三甲散加减

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：