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Cd Se/Zn S quantum dots induce photodynamic effects and cytotoxicity in pancreatic cancer cells

World Journal of Gastroenterology 2016年第21期22卷 5012-5022页

作者： si-jia he jia Cao Yong-Sheng Li jia-Chun Yang Min Zhou Chun-Ying Qu Yi Zhang Feng Shen Ying Chen Ming-Ming Li Lei-Ming Xu Department of Gastroenterology Xinhua Hospital School of Medicine Shanghai Jiaotong University the Comprehensive Cancer Center and Shanghai Key Laboratory for Pancreatic Diseases Shanghai General Hospital Shanghai Jiao Tong University School of Medicine Department of General Surgery Xinhua Hospital School of Medicine Shanghai Jiaotong University

AIM: To investigate the photodynamic effect of Cd Se/Zn S quantum dots(QDs) on pancreatic cancer cells and elucidate the probable ***: The pancreatic cancer cell line SW1990 was treated with different concentrations of Cd Se/Zn S QDs(0, 0.5, 1.0, 1.5, 2.0, 2.5 μmol/L), with or without illumination. The viability of SW1990 cells was tested using the Cell Counting Kit-8(CCK-8) assay. The ultrastructural changes of SW1990 cells were observed by transmission electron microscopy. Apoptosis was detected by nuclear staining and flow cytometry(FCM). Reactive oxygen species(ROS) were measured by dichlorofluorescein diacetate via fluorescence microscopy. Expression of Bax, Bcl-2 and caspase-3 was measured by real-time polymerase chain reaction(PCR) and protein immunoblotting 24 h after SW1990 cells were treated with Cd Se/Zn S QDs and ***: The CCK-8 assay results showed that both Cd Se/Zn S QDs with and without illumination suppressed SW1990 cell proliferation. Cell viability was significantly lower when illuminated or with a longer incubation time and a higher light dose. Cd Se/Zn S QDs with illumination caused ultrastructural changes in SW1990 cells, such as organelle degeneration and chromatin condensation and aggregation at the periphery of the nucleus. Fluorescence microscopy and FCM showed that Cd Se/Zn S QDs(1.5 μmol/L) with illumination increased SW1990 cell apoptosis(53.2%) and ROS generation compared with no illumination. Real-time PCR showed that expression of Bax and caspase-3 was upregulated and Bcl-2 was downregulated. Immunoblotting results were consistent with real-time PCR results. Inhibition of ROS and apoptosis both attenuated QD-photodynamictherapy-induced cell ***: Cd Se/Zn S QDs can be used as a photosensitizer to inhibit SW1990 cell proliferation through ROS generation and apoptotic protein expression regulation.

关键词： Quantum dots Pancreatic cancer Apoptosis Photodynamic therapy Reactive oxygen species

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single-Entity Collisional Electrochemistry at the Micro-and/or Nano-Interface Between Two Immiscible Electrolyte Solutions

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电化学（中英文） 2024年第11期30卷 1-16,I0001页

作者： Li-Fang Yang Jun-Jie Chen Ling-Yu Chen si-Qi Jin Tao-Xiong Fang si-jia he Liang-Jun Shen Xin-jian Huang Xiao-Hang Sun Hai-Qiang Deng School of Chemical Engineering and Technology Sun Yat-sen UniversityZhuhai 519082China Institute of Intelligent Technology Midea Corporate Research CenterFoshan 528311China

single-entity collisional electrochemistry(SECE)is a branch of single-entity *** can directly characterize entities/particles with single particle resolution through random collisions between particles and electrodes in a solution,and obtain rich physicochemical information,thus becoming one of the frontiers of electroanalytical chemistry in the past two ***,the(micro/nanoscale)sensing electrodes have evolved from a polarizable liquid/liquid(mercury/liquid)interface to a solid/liquid interface and then to a liquid/liquid interface(i.e.,an interface between twoimmiscible electrolyte solutions,ITIES),as if they have completed a cycle(but in fact they have not).ITIES has become the latest sensing electrode in the booming SECE due to its polarizability(up to 1.1 V at the water/a,a,a-trifluorotoluene interface)and high *** four measurement modes(direct electrolysis,mediated electrolysis,current blockade,and charge displacement)developed in the realm of SECE at solid/liquid interfaces have also been fully realized at the miniature *** article will discuss these four modes at the ITIES from the perspectives of basic concepts,operating mechanisms,and latest developments(e.g.,discovery of ionosomes,blockade effect of Faradaic ion transfer,etc.),and look forward to the future development and direction of this emerging field.

关键词： single-entity collisional electrochemistry Interface between two immiscible electrolyte solutions Charge transfer

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Effects of infrasound exposure on micronucleus of bone marrow polychromatic erythroblasts in mice

Effects of infrasound exposure on micronucleus of bone marro...

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中国细胞生物学学会第八届会员代表大会暨学术大会

作者： ZHANG si-he, jia Ke-Yang, SHU Qing, ZHANG Qing, LIU Su-Ying, CheN Jing-Zao Department of Cell Biology , Department of Physiotherapy and Rehabilitation , Xi jing Hospital, Department of Clinical Laboratory, Tangdu Hospital, Fourth Military Medical University, Xi’an 710033, China

AIM To study the damage of chromosome in bone marrow cell of mice exposed to infrasound. METHODS BALB/C mice were exposed to infrasound of 16 Hz, 120 dB> 2 hours per day. Then femora were obtained after1, 3, 7, 10 days respectively. The rates of micronucleus (MN) in polychromatophilic erythroblast (PCE) were detected by PCE-MNT and the chromosomes in division phase were observed. RESULTS The mean rate of MN in infrasound group increased markedly compared with the control group (Phe infrasound exposure day prolonging, the mean rates of MN in infrasound group increased significantly (KO. 05). No significant difference of the mean rates of MN were detected between the male mice and the female mice (P>0. 05) . Visible structural aberration was observed distinctly in division phase of bone marrow cells. CONCLUsiON Genetic damage to PCE of mice by the infrasound exposure was detected significantly. Accumulative effect existed in infrasound exposure days, whereas no gender difference on sensitivity to infrasound exposure existed.

关键词： Infrasound Micronucleus Polychromatophilic erythroblast

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Viscosity and structure relationship with equimolar substitution of CaO with MgO in the CaO–MgO–Al_(2)O_(3)–siO_(2)slag melts

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International Journal of Minerals,Metallurgy and Materials 2025年第1期32卷 70-79页

作者： Yong Hou Shuo Zhang Jie Dang jia Guo Hanghang Zhou Xuewei Lü College of Materials Science and Engineering Chongqing UniversityChongqing 400044China Chongqing Key Laboratory of Vanadium-Titanium Metallurgy and New Materials Chongqing UniversityChongqing 400044China

Currently,the Al_(2)O_(3)content in the high-alumina slag systems within blast furnaces is generally limited to 16wt%–18.5wt%,making it challenging to overcome this *** most studies that concentrated on managing the MgO/Al_(2)O_(3)ratio or basicity,this paper explored the effect of equimolar substitution of MgO for CaO on the viscosity and structure of a high-alumina CaO-MgO-Al_(2)O_(3)-siO_(2)slag system,providing theoretical guidance and data to facilitate the application of high-alumina *** results revealed that the viscosity first decreased and then increased with higher MgO substitution,reaching a minimum at 15mol%MgO *** transform infrared spectroscopy(FTIR)results found that the depths of the troughs representing[siO_(4)]tetrahedra,[AlO_(4)]tetrahedra,and si-O-Al bending became progressively deeper with increased MgO *** of the Raman spectra showed that the average number of bridging oxygens per si atom and the X_(Q^(3))/X_(Q^(2))(X_(Q^(i))is the molar fraction of Q^(i) unit,and i is the number of bridging oxygens in a[siO_(4)]tetrahedral unit)ratio increased from 2.30 and 1.02 to 2.52 and 2.14,respectively,indicating a progressive polymerization of the silicate structure.X-ray photoelectron spectroscopy(XPS)results highlighted that non-bridging oxygen content decreased from 77.97mol% to 63.41mol% with increasing MgO concentration,whereas bridging oxygen and free oxygen contents *** analysis demonstrated a gradual increase in the polymerization degree of the tetrahedral structure with the increase in MgO ***,bond strength is another important factor affecting the slag *** occurrence of a viscosity minimum can be attributed to the complex evolution of bond strengths of non-bridging oxygens generated during depolymerization of the[siO_(4)]and[AlO_(4)]tetrahedral structures by CaO and MgO.

关键词： aluminosilicate viscosity structure spectroscopy

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不同管径二氧化钛纳米管对骨髓间充质干细胞生物学行为的影响

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口腔医学研究 2017年第7期33卷 737-741页

作者：许志强黄斯佳贺于奇邹耿森邱文陈江莆田学院附属医院口腔科福建莆田351100 泉州医学高等专科学校福建泉州362000 福建医科大学附属口腔医院种植科福建福州350004

目的:研究不同管径二氧化钛纳米管(TNTs)对骨髓间充质干细胞(BMSCs)生物学行为的影响。方法:阳极氧化法分别制备管径30、100和200nm的TNTs,MTT法检测细胞在材料表面粘附及增殖情况,碱性磷酸酶活性、胶原分泌量和细胞外基质矿化水平检测... 详细信息

目的:研究不同管径二氧化钛纳米管(TNTs)对骨髓间充质干细胞(BMSCs)生物学行为的影响。方法:阳极氧化法分别制备管径30、100和200nm的TNTs,MTT法检测细胞在材料表面粘附及增殖情况,碱性磷酸酶活性、胶原分泌量和细胞外基质矿化水平检测来评估细胞的成骨分化能力。结果:30nm的TNTs促进BMSCs的早期粘附和增殖,但是其后期细胞增殖和成骨分化能力同对照组相比没有明显的统计学差异;200nm的TNTs明显促进了BMSCs的成骨分化,但是其细胞粘附和增殖明显受到了抑制;100nm的TNTs上BMSCs的早期粘附和增殖受到了轻微的抑制,但是随着时间的推移,其细胞增殖迎头赶上,而且其成骨分化能力显著大于对照组。结论:最适BMSCs生物学活性的应该是100nm的TNTs。

关键词：二氧化钛纳米管骨髓间充质干细胞细胞粘附细胞增殖成骨分化

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基于BP神经网络的电动扳手扭矩校准研究

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制造业自动化 2024年第5期46卷 48-53页

作者：钱龙方笑晗斯嘉禾吴欢乐范学伟安徽大学电气工程与自动化学院安徽合肥230601

针对定扭矩电动扳手输出扭矩值与设定扭矩值不一致产生的非线性误差问题,提出使用BP神经网络建立扭矩参数校准模型。实验结果表明,与分段线性插值法和最小二乘法相比,BP神经网络对非线性曲线拟合程度更好。同时设计在线校准程序,将扭矩... 详细信息

针对定扭矩电动扳手输出扭矩值与设定扭矩值不一致产生的非线性误差问题,提出使用BP神经网络建立扭矩参数校准模型。实验结果表明,与分段线性插值法和最小二乘法相比,BP神经网络对非线性曲线拟合程度更好。同时设计在线校准程序,将扭矩校准模型应用在扳手实际工作过程中,经试验测量,该校准模型使得扳手扭矩输出精度控制在±3%以内,具有一定的适应性和实用性。

关键词：电动扳手扭矩校准非线性 BP神经网络

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Role of mountains and rivers in the formation of logistics enterprises'spatial pattern in the central urban areas of Chongqing

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Journal of Mountain Science 2022年第7期19卷 2060-2074页

作者： LIU si-jing ZHU Cheng-jia he Nan-nan LI Guo-qi School of Transportation and Logistics Southwest Jiaotong UniversityChengdu611756China National Engineering Laboratory of Integrated Transportation Big Data Application Technology Chengdu611756China National United Engineering Laboratory of Integrated and Intelligent Transportation Chengdu611756China

The complex landforms of a Shan-shui City(Shan-shui refers to mountains and rivers)significantly impact the selection of locations for logistics *** paper takes Chongqing,one of the most typical Shan-shui Cities in China,as the research object,and adopts spatial analysis methods and a mediating effect model,to explore the role of mountains and rivers in the formation of logistics enterprises’spatial pattern on the street *** study results reveal that 90% of the logistics enterprises in the central urban areas of Chongqing are located in the low-altitude area below 353m above sea level,and distributed in a north-south direction along the mountains,as a result of blockage by mountain ranges,such as those of Zhongliang Mountain and Tongluo *** than 70% of the logistics enterprises are located less than 5 km from either the Yangtze River or jialing River,spreading along the *** addition,more than half of the logistics enterprises in commercial and financial,and residential land are located within the urban core area,while 80.83% of the logistics enterprises located in warehousing land and industrial land are concentrated in the urban expansion *** areas with high land prices,the negative effect of altitude on logistics enterprise agglomeration is weakened,while the promotion effect of river proximity on logistics enterprise agglomeration is *** the urban core area with the advantage of low altitude and proximity to the jialing and Yangtze Rivers,the role of mountains and rivers on logistics enterprises is not apparent;in contrast,in the urban expansion area with more complex landforms,land price can be an effective means for the government to macro-manage the spatial pattern of logistics enterprises in a Shan-shui City.

关键词： Logistics enterprise Spatial pattern Influencing factors Driving mechanism Chongqing

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Rogue wave solutions and rogue-breather solutions to the focusing nonlinear Schr?dinger equation

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Communications in Theoretical Physics 2024年第3期76卷 33-41页

作者： si-jia Chen Xing Lü College of Science Tianjin University of TechnologyTianjin 300384People's Republic of China Department of Mathematics Beijing Jiaotong UniversityBeijing 100044People's Republic of China Beijing Laboratory of National Economic Security Early-warning Engineering Beijing Jiaotong UniversityBeijing 100044People's Republic of China

Based on the long wave limit method,the general form of the second-order and third-order rogue wave solutions to the focusing nonlinear Schr?dinger equation are given by introducing some arbitrary *** interaction solutions between the first-order rogue wave and one-breather wave are constructed by taking a long wave limit on the two-breather *** applying the same method to the three-breather solutions,two types of interaction solutions are obtained,namely the first-order rogue wave and two breather waves,the second-order rogue wave and one-breather wave,*** influence of the parameters related to the phase on the interaction phenomena is graphically *** occur among the rogue waves and breather *** the collisions,the shape of them remains *** abundant interaction phenomena in this paper will contribute to a better understanding of the propagation and control of nonlinear waves.

关键词： nonlinear Schrodinger equation rogue wave solutions interaction solutions

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Road map for, and technical challenges of, carbon-nanotube integrated circuit technology

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National Science Review 2024年第3期11卷 14-16页

作者： jia si Panpan Zhang Zhiyong Zhang Key Laboratory for the Physics and Chemistry of Nanodevices and Center for Carbon-based Electronics School of Electronics Peking University State Key Laboratory of Information Photonics and Optical Communications Beijing University of Posts and Telecommunications

The past decade has witnessed an explosion of research on carbon-nanotube(CNT) electronics due to their outstanding properties, including an ultrathin body, a high carrier mobility and a high saturation velocity.

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Cloning, bioinformatics and transcriptional analysis of caffeoyl-coenzyme A 3-O-methyltransferase in switchgrass under abiotic stress

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Journal of Integrative Agriculture 2016年第3期15卷 636-649页

作者： LIU si-jia HUANG Yan-hua he Chang-jiu FANG Cheng ZHANG Yun-wei Department of Grassland Science College of Animal Science and Technology China Agricultural University/National Energy R&D Center for Biomass (NECB) Key Laboratory of Animal Genetics and Breeding Ministry of Agriculture/National Engineering Laboratory for Animal Breeding College of Animal Science and Technology China Agricultural University

Genes encoding enzymes involved in the lignin biosynthesis through phenylpropanoid pathway were not only associated with the lignin content, but also related to the abiotic stress resistances. As far as the production of liquid biofuels and cultivation within the marginal land are concerned, switchgrass could be the better candidate to determine the relationship between lower lignin content and physiological function under stress. Caffeoyl-coenzyme A 3-O-methyltransferase（CCoAOMT） is a key enzyme for the methylation reaction of lignin biosynthesis. For this purpose, we cloned a CCoAOMT gene from switchgrass and identified its expression patterns under abiotic stresses. The full-length CCoAOMT gene, designated PvCCoAOMT（Gen Bank accession no. KF041775）, was 1 005-bp in length, has an opening reading frame of 777 nucleotides encoding a 258-amino acid protein. The deduced amino acid sequence of PvCCoAOMT shared a high degree of similarity（up to 98%） with CCoAOMTs from Panicum virgatum allele（BAO20881）, Sorghum bicolor（XP002436550） and Zea mays（NP001131288）. Using quantitative real-time PCR（qRT-PCR）, the significant upregulation of PvCCoAOMT was observed in stem tissues at a later stage（24 h） after drought treatment, with the transcript level increasing 33-fold compared that of the controls. Moderate and insignificant inductions of PvCCoAOMT were also observed in both stems and leaves during the later stages after cold（48 h in stems, 12 h in leaves） and mechanical wounding（48 h in stems, 12 h in leaves） treatments, respectively. Our results showed the different expression patterns of PvCCoAOMT in drought, cold and mechanical wounding stresses. PvCCoAOMT can be highly induced by drought and cold stresses, which indicates that it may play a role in plant abiotic stress resistance, particularly in the regulation of drought and cold resistance.

关键词： Panicum virgatum phenylpropanoid drought resistance biofuel production real-time PCR

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