Cd Se/Zn S quantum dots induce photodynamic effects and cytotoxicity in pancreatic cancer cells

作     者：Si-Jia He Jia Cao Yong-Sheng Li Jia-Chun Yang Min Zhou Chun-Ying Qu Yi Zhang Feng Shen Ying Chen Ming-Ming Li Lei-Ming Xu 

作者机构：Department of Gastroenterology Xinhua Hospital School of Medicine Shanghai Jiaotong University the Comprehensive Cancer Center and Shanghai Key Laboratory for Pancreatic Diseases Shanghai General Hospital Shanghai Jiao Tong University School of Medicine Department of General Surgery Xinhua Hospital School of Medicine Shanghai Jiaotong University 

出 版 物：《World Journal of Gastroenterology》 (世界胃肠病学杂志（英文版）)

年 卷 期：2016年第22卷第21期

页      面：5012-5022页

核心收录：

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

基　　金：Supported by the National Natural Science Foundation of China,No.81472844 and No.81301826 Shanghai Municipal Education Commission,No.14ZZ114 

主　　题：Quantum dots Pancreatic cancer Apoptosis Photodynamic therapy Reactive oxygen species 

摘      要：AIM: To investigate the photodynamic effect of Cd Se/Zn S quantum dots(QDs) on pancreatic cancer cells and elucidate the probable ***: The pancreatic cancer cell line SW1990 was treated with different concentrations of Cd Se/Zn S QDs(0, 0.5, 1.0, 1.5, 2.0, 2.5 μmol/L), with or without illumination. The viability of SW1990 cells was tested using the Cell Counting Kit-8(CCK-8) assay. The ultrastructural changes of SW1990 cells were observed by transmission electron microscopy. Apoptosis was detected by nuclear staining and flow cytometry(FCM). Reactive oxygen species(ROS) were measured by dichlorofluorescein diacetate via fluorescence microscopy. Expression of Bax, Bcl-2 and caspase-3 was measured by real-time polymerase chain reaction(PCR) and protein immunoblotting 24 h after SW1990 cells were treated with Cd Se/Zn S QDs and ***: The CCK-8 assay results showed that both Cd Se/Zn S QDs with and without illumination suppressed SW1990 cell proliferation. Cell viability was significantly lower when illuminated or with a longer incubation time and a higher light dose. Cd Se/Zn S QDs with illumination caused ultrastructural changes in SW1990 cells, such as organelle degeneration and chromatin condensation and aggregation at the periphery of the nucleus. Fluorescence microscopy and FCM showed that Cd Se/Zn S QDs(1.5 μmol/L) with illumination increased SW1990 cell apoptosis(53.2%) and ROS generation compared with no illumination. Real-time PCR showed that expression of Bax and caspase-3 was upregulated and Bcl-2 was downregulated. Immunoblotting results were consistent with real-time PCR results. Inhibition of ROS and apoptosis both attenuated QD-photodynamictherapy-induced cell ***: Cd Se/Zn S QDs can be used as a photosensitizer to inhibit SW1990 cell proliferation through ROS generation and apoptotic protein expression regulation.