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Cloning and Characterization of Putative Hd6 Ortholog Associated with Zea mays L. Photoperiod Sensitivity

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Agricultural Sciences in China 2011年第1期10卷 18-27页

作者： KU Li-xia LI si-yuan CHEN Xiao wu Lian-cheng WANG Xin-tao CHEN Yan-hui College of Agronomy Henan Agricultural University Zhengzhou 450002 P.R.China

The photoperiod response of lowering is critical to the survival and successful reproduction in crops. The Hd6 gene, encoding a protein similar to the Arabidopsis α-subunit of CASEIN KINASE2α （CK2α） has been reported to affect flowering time through the photoperiodic pathway in rice. In this study, the maize ortholog, ZmHd6, has been cloned and its role in the photoperiodic pathway was investigated. ZmHd6 encodes 332 amino acids with six distinct domains： two CK2 active domains, a nuclear localization signal sequence, a N terminal domain, an ATP binding site, and an active site of serine/threonine protein kinase. ZmHd6 was constitutively expressed in the leaves and shoot apical meristem （SAM） with the highest expression in SAMs of plants with seven leaves under long and short day conditions. Delayed flowering time under long day （LD） conditions were observed in ZmHd6 overexpression transgenic Arabidopsis plants compared to the wild type. In transgenic Arabidopsis plants, ZmHd6 was expressed in a circadian oscillatory pattern, TOC1 peak expression was delayed, new GI, CO, and FT expressions reached to peak after 3 h while entering illumination period and oscillatory expression patterns of CO and FT were created during the dark phase. These results suggested that ZmHd6 might negatively regulate flowering time under LD conditions by delaying TOC1 peak expression and disrupting CO and FT expression in transgenic Arabidopsis plants.

关键词： maize (Zea mays L.) ZmHd6 photoperiod response expression

Comprehensive risk assessment for early neurologic complications after liver transplantation

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World Journal of Gastroenterology 2016年第24期22卷 5548-5557页

作者： si-yuan wu Teng-Wei Chen An-Chieh Feng Hsiu-Lung Fan Chung-Bao Hsieh Kuo-Piao Chung Division of Organ Transplantation Surgery Department of Surgery Tri-Service General Hospital National Defense Medical Center Institute of Health Policy and Management College of Public Health National Taiwan University

AIM: To determine risk factors for early neurologic complications(NCs) after liver transplantation from perspective of recipient, donor, and surgeon. METHODS: In all, 295 adult recipients were enrolled consecutively between August 2001 and February 2014 from a single medical center in Taiwan. Any NC in the first 30 d post-liver transplantation, and perioperative variables from multiple perspectives were collected and analyzed. The main outcome was a 30-d NC. Generalized additive models were used to detect the non-linear effect of continuous variables on outcome, and to determine cut-off values for categorizing risk. Risk factors were identified using multiple logistic regression analysis. RESULTS: In all, 288 recipients were included, of whom 142(49.3%) experienced at least one NC, with encephalopathy being the most common 106(73%). NCs prolonged hospital stay(35.15 ± 43.80 d vs 20.88 ± 13.58 d, P 27.6 kg/m^2, Child-Pugh class C, history of preoperative hepatoencephalopathy or mental disorders, day 7 tacrolimus level > 8.9 ng/m L, and postoperative intraabdominal infection were more likely associated with NCs. Novel risk factors for NCs were donor age siON: NCs post- liver transplantation occurs because of factors related to recipient, donor, and surgeon. Our results provide a basis of risk stratification for surgeon to minimize neurotoxic factors during transplantation.

关键词： Risk Liver transplantation Neurotoxicity syndromes Donor Learning curve

Evaluating modified effects of family factors on intervention to protect primary school students from the impact of heatwave

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Advances in Climate Change Research 2023年第6期14卷 866-872页

作者： Rui ZHANG Bo SUN si-yuan wu Shui-Ling QU Yan WANG Yu-Jie MENG Song-Wang WANG Xiao-yuan YAO Shilu TONG Yong-Hong LI Yi-Bin CHENG Chinese Center for Disease Control and Prevention Bejing 102206China National Institute of Environmental Health Chinese Center for Disease Control and PreventionBeijing 100021China China CDC Key Laboratory of Environment and Population Health National Institute of Environmental HealthChinese Center for Disease Control and PreventionBeijing 100021China Sprott School of Businss Carleton UniversityOttawa Ontario KIS 5B6Canada School of Public Health and Social Work Queensland University of Technology BrisbaneBrisbane 4006Australia

Identifying the factors that may impact intervention effectiveness of primary students is crucial for developing targeted intervention strategies on heatwave *** purpose of the study was to assess how the family factors could affect the intervention effects of primary school students to adapt heatwave,using the data from a previously conducted primary school-based intervention *** study recruited 648 students(9-13 years old)and their parents from two primary schools in Dongtai of Jiangsu province,*** May to September 2017,a 5-mon intervention for enhancing knowledge,attitude and practice(KAP)was implemented at the intervention *** students'KAP scores and the family-related information were measured by designed self-administered questionnaires at intervention school and control school separately before and after *** effects of the intervention were explored through the propensity score matching and difference-in-difference method(PSM-DID),and stratifed analysis were conducted by family *** overall KAP score increased by 2.391(95%CI:1.881,2.902)after intervention,which shows that the intervention was *** students whose parents with higher school educational attainment,parents with employment,and household number≤3 achieved significantly higher increment of KAP scores than their *** students whose parents in marriage achieved higher score increasing than parents not in marriage,the difference was *** findings indicated that parental education status,working status and the household size were the influence factors of heatwave adaption interventions for primary school *** interventions should take parental education status,working status and household size into account to improve the effectiveness of heatwave adaption intervention for primary school students.

关键词： Heatwave Intervention Family factors Primary students Parents

Clinical application of hepatic venous occlusion for hepatectomy

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Chinese Medical Journal 2008年第9期121卷 806-810页

作者： PAN Ze-ya YANG yuan ZHOU Wei-ping LI Ai-jun FU si-yuan wu Meng-chao The Third Department of Hepatic Surgery Eastern Hepatobiliary Surgery Hospital Second Military Medical University Shanghai 200438 China

Background Most liver resections require clamping of the hepatic pedicle （Pringle maneuver） to avoid excessive blood loss. But Pringle maneuver can not control backflow bleeding of hepatic vein. Resection of liver tumors involving hepatic veins may cause massive hemorrhage or air embolism from the injuries of the hepatic veins. Although total hepatic vascular exclusion can prevent bleeding of the hepatic veins effectively, it also may result in systemic hemodynamic disturbance because of the inferior vena cava being clamped. Hepatic venous occlusion, a new technique, can control the inflow and outflow of the liver without clamping the vena cava. Methods A total of 71 cases of liver tumors underwent resection with occlusion of more than one of the main hepatic veins. All tumors involved the second porta hepatis and at least one main hepatic vein. Ligation or occlusion with serreflnes, tourniquets and auricular clamps were used in hepatic venous occlusion. Results Of the 71 patients, ligation of the hepatic veins was used in 28 cases, occlusion with a tourniquet in 26, and occlusion with a serrefine in 17. Right hepatic veins were occluded in 38 cases, both right and middle hepatic veins in 2, the common trunk of the left and middle hepatic veins in 24, branches of the left and middle hepatic veins in 2, and all three hepatic veins in 5. Thirty-five cases underwent hemihepatic vascular occlusion, 4 alternate hemihepatic vascular occlusion, 23 portal triad clamping plus selective hepatic vein occlusion, and 9 portal triad clamping plus total hepatic vein occlusion. The third porta hepatis was isolated in 26 cases. The amount of intraoperative blood loss averaged （540±2.83）（range 100 to 1000） ml in the group of total hemihepatic vascular occlusion and in the group of alternate hemihepatic vascular occlusion, （620±317）（range 200-6000） ml in the group of portal triad clamping plus selective or total hepatic vein occlusion. All tumors were completely removed. Conclusions

关键词： liver neoplasms hepatic veins hepatectomy hemorrhage

电纺压电聚偏二氟乙烯有序纳米纤维及其在压力传感器中的应用

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光学精密工程 2016年第10期24卷 2498-2504页

作者：王凌云马思远吴德志厦门大学机电工程系福建厦门361000

针对传统静电纺丝法制备聚偏二氟乙烯（PVDF）压电纤维工艺复杂和效率低等问题,提出了采用滚筒收集方式制备PVDF有序纳米纤维的方法。通过改变滚筒转速收集纤维,得到了有序程度不同的PVDF纤维膜。用傅立叶变换红外光谱（FTIR）分析了有... 详细信息

针对传统静电纺丝法制备聚偏二氟乙烯（PVDF）压电纤维工艺复杂和效率低等问题,提出了采用滚筒收集方式制备PVDF有序纳米纤维的方法。通过改变滚筒转速收集纤维,得到了有序程度不同的PVDF纤维膜。用傅立叶变换红外光谱（FTIR）分析了有序纤维膜β晶相的含量,并利用NI数据采集卡在相同条件下测试了纤维膜的压电特性。结果表明：随着滚筒转速提高,纤维有序度增强,β相的含量提高,压电电压输出也明显增大,由此得知滚筒转速变化对β晶相的含量及压电输出电压的影响规律基本一致。基于得到的结果,设计制作了利用该有序PVDF纤维膜的压力传感器,并在不同气压下进行了动态响应测试。结果显示：所制备的PVDF有序纤维压力传感器在0.145-0.165 MPa下的电压输出随气压的增加呈线性增大,表现出了良好的线性度,其灵敏度达179mV/kPa。采用该种方法制备PVDF纤维对研制动态压力传感器极有意义。

关键词：静电纺丝滚筒收集聚偏二氟乙烯有序纳米纤维压电特性压力传感器

Unlocking the potential of spatial transcriptomics with custom microfluidic chips

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Science China Life Sciences 2025年

作者： Bingqi wu yuan Gao China National Center for Bioinformation Beijing Institute of Genomics Chinese Academy of Sciences University of Chinese Academy of Sciences

Spatial transcriptomics technique detects RNA sequences (St?hl et al.,2016) and quantifies their expression in the positional context (Ke et al.,2013),which can provide important information such as cell heterogeneity,cell developmental trajectory,differential gene expression between tissue regions or *** date,it is becoming a powerful tool for exploring tumorigenesis (Ren et al.,2023),cerebral neurodevelopment (Shi et al.,2023),embryonic development(Arutyunyan et al.,2023),and other biological ***-dimensional(3D) spatial transcriptome typically requires stacking multiple tissue slices to obtain a 3D atlas (Schott et al.,2024);therefore it prefers a method that meets high throughput,large sample sizes,and affordable cost to cover large regions of the ***,the lack of a single method that meets all of these requirements limits large-scale application of 3D spatial transcriptomics.

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Dynamic Regulation of Hydrogen Bonding Networks and Solvation Structures for Synergistic Solar‑Thermal Desalination of Seawater and Catalytic Degradation of Organic Pollutants

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Nano-Micro Letters 2025年第2期17卷 548-565页

作者： Ming‑yuan Yu Jing wu Guang Yin Fan‑Zhen Jiao Zhong‑Zhen Yu Jin Qu Beijing Key Laboratory of Advanced Functional Polymer Composites Beijing University of Chemical TechnologyBeijing 100029People’s Republic of China State Key Laboratory of Organic‑Inorganic Composites College of Materials Science and EngineeringBeijing University of Chemical TechnologyBeijing 100029People’s Republic of China

Although solar steam generation strategy is efficient in desalinating seawater,it is still challenging to achieve continuous solar-thermal desalination of seawater and catalytic degradation of organic ***,dynamic regulations of hydrogen bonding networks and solvation structures are realized by designing an asymmetric bilayer membrane consisting of a bacterial cellulose/carbon nanotube/Co_(2)(OH)_(2)CO_(3)nanorod top layer and a bacterial cellulose/Co_(2)(OH)_(2)CO_(3)nanorod(BCH)bottom ***,the hydrogen bonding networks inside the membrane can be tuned by the rich surface–OH groups of the bacterial cellulose and Co_(2)(OH)_(2)CO_(3)as well as the ions and radicals in situ generated during the catalysis ***,both SO_(4)^(2−)and HSO_(5)−can regulate the solvation structure of Na^(+)and be adsorbed more preferentially on the evaporation surface than Cl^(−),thus hindering the de-solvation of the solvated Na^(+)and subsequent nucleation/growth of ***,the heat generated by the solar-thermal energy conversion can accelerate the reaction kinetics and enhance the catalytic degradation *** work provides a flow-bed water purification system with an asymmetric solar-thermal and catalytic membrane for synergistic solar thermal desalination of seawater/brine and catalytic degradation of organic pollutants.

关键词： Solar steam generation Seawater desalination Catalytic degradation Bacterial cellulose Cobalt hydroxycarbonate nanorods

“Zero‑Strain” NiNb_(2)O_(6) Fibers for All‑Climate Lithium Storage

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Nano-Micro Letters 2025年第1期17卷 348-360页

作者： Yan Zhao Qiang yuan Liting Yang Guisheng Liang Yifeng Cheng Limin wu Chunfu Lin Renchao Che College of Physics Donghua UniversityShanghai 201620People’s Republic of China School of Materials Science and Engineering Institute of Materials for Energy and EnvironmentQingdao UniversityQingdao 266071People’s Republic of China Laboratory of Advanced Materials Shanghai Key Laboratory of Molecular Catalysis and Innovative MaterialsAcademy for Engineering&TechnologyFudan UniversityShanghai 200438People’s Republic of China Zhejiang Laboratory Hangzhou 311100People’s Republic of China Inner Mongolia University Hohhot 010021People’s Republic of China

Niobates are promising all-climate Li^(+)-storage anode material due to their fast charge transport,large specific capacities,and resistance to electrolyte ***,their moderate unit-cellvolume expansion(generally 5%–10%)during Li^(+)storage causes unsatisfactory long-term ***,“zero-strain”NiNb_(2)O_(6) fibers are explored as a new anode material with comprehensively good electrochemical *** Li^(+)storage,the expansion of electrochemical inactive NiO_(6) octahedra almost fully offsets the shrinkage of active NbO_(6) octahedra through reversible O *** superior volume-accommodation capability of the NiO_(6) layers guarantees the“zero-strain”behavior of NiNb_(2)O_(6) in a broad temperature range(0.53%//0.51%//0.74%at 25//−10//60℃),leading to the excellent cyclability of the NiNb_(2)O_(6) fibers(92.8%//99.2%//91.1%capacity retention after 1000//2000//1000 cycles at 10C and 25//−10//60℃).This NiNb_(2)O_(6) material further exhibits a large reversible capacity(300//184//318 mAh g−1 at 0.1C and 25//−10//60℃)and outstanding rate performance(10 to 0.5C capacity percentage of 64.3%//50.0%//65.4%at 25//−10//60℃).Therefore,the NiNb_(2)O_(6) fibers are especially suitable for large-capacity,fast-charging,long-life,and all-climate lithium-ion batteries.

关键词： NiNb_(2)O_(6)porous fiber “Zero-strain”mechanism Electrochemical property Harsh-temperature operation Operando characterization

Study on the hydrogen absorption properties of a YGdTbDyHo rare-earth high-entropy alloy

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International Journal of Minerals,Metallurgy and Materials 2025年第1期32卷 127-135页

作者： Tongyue Li Ziliang Xie Wenjiao Zhou Huan Tong Dawen Yang Anjia Zhang yuan wu Xiping Song State Key Laboratory for Advanced Metals and Materials University of Science and Technology BeijingBeijing 100083China Whole Win(Beijing)Materials Sci.&Tech.Co. Ltd.Beijing 100083China

This study investigated the microstructure and hydrogen absorption properties of a rare-earth high-entropy alloy(HEA),*** indicated that the YGdTbDyHo alloy had a microstructure of equiaxed grains,with the alloy elements distributed *** hydrogen absorption,the phase structure of the HEA changed from a solid solution with an hexagonal-close-packed(HCP)structure to a high-entropy hydride with an faced-centered-cubic(FCC)structure without any secondary phase *** alloy demonstrated a maximum hydrogen storage capacity of 2.33 H/M(hydrogen atom/metal atom)at 723 K,with an enthalpy change(ΔH)of-141.09 kJ·mol^(-1)and an entropy change(ΔS)of-119.14 J·mol^(-1)·K^(-1).The kinetic mechanism of hydrogen absorption was hydride nucleation and growth,with an apparent activation energy(E_(a))of 20.90 kJ·mol^(-1).Without any activation,the YGdTbDyHo alloy could absorb hydrogen quickly(180 s at 923 K)with nearly no incubation period *** reason for the obtained value of 2.33 H/M was that the hydrogen atoms occupied both tetrahedral and octahedral *** results demonstrate the potential application of HEAs as a high-capacity hydrogen storage material with a large H/M ratio,which can be used in the deuterium storage field.

关键词： rare-earth high-entropy alloy hydrogen absorption capacity pressure–composition–temperature curves kinetics