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Inhibition of EGFR attenuates EGF-induced activation of retinal pigment epithelium cell via EGFR/AKT signaling pathway

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International Journal of Ophthalmology(English edition) 2024年第6期17卷 1018-1027页

作者： Yu-Sheng Zhu si-rui zhou Hui-Hui Zhang Tong Wang Xiao-Dong Chen Faculty of Life Sciences and Medicine Northwest UniversityXi’an 710069Shaanxi ProvinceChina First Affiliated Hospital of Northwest University Northwest UniversityXi’an 710069Shaanxi ProvinceChina Department of Ophthalmology Xi’an No.1 HospitalXi’an 710002Shaanxi ProvinceChina Shaanxi Institute of Ophthalmology Shaanxi Provincial Key Lab of OphthalmologyClinical Research Center for Ophthalmology Diseases of Shaanxi ProvinceXi’an 710002Shaanxi ProvinceChina

AIM:To explore the effect of epidermal growth factor receptor(EGFR)inhibition by erlotinib and EGFR siRNA on epidermal growth factor(EGF)-induced activation of retinal pigment epithelium(RPE)***:Human RPE cell line(ARPE-19 cells)was activated by 100 ng/mL *** and EGFR siRNA were used to intervene EGF *** viability,proliferation,and migration were detected by methyl thiazolyl tetrazolium(MTT)assay,bromodeoxyuridine(BrdU)staining assay and wound healing assay,***/protein kinase B(AKT)pathway proteins and N-cadherin,α-smooth muscle actin(α-SMA),and vimentin were tested by Western blot *** was also determined by immunofluorescence ***:EGF treatment for 24h induced a significant increase of ARPE-19 cells’viability,proliferation and migration,phosphorylation of EGFR/AKT proteins,and decreased total EGFR *** suppressed ARPE-19 cells’viability,proliferation and migration through down regulating total EGFR and AKT protein *** also inhibited EGF-induced an increase of proliferative and migrative ability in ARPE-19 cells and clearly suppressed EGF-induced EGFR/AKT proteins phosphorylation and decreased expression of N-cadherin,α-SMA,and vimentin ***,EGFR inhibition by EGFR siRNA significantly affected EGF-induced an increase of cell proliferation,viability,and migration,phosphorylation of EGFR/AKT proteins,and up-regulation of N-cadherin,α-SMA,and vimentin ***:Erlotinib and EGFR-knockdown suppress EGF-induced cell viability,proliferation,and migration via EGFR/AKT pathway in RPE *** inhibition may be a possible therapeutic approach for proliferative vitreoretinopathy(PVR).

关键词： erlotinib epidermal growth factor receptor protein kinase B epithelial-mesenchymal transition retinal pigment epithelium cell

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Potassium voltage-gated channel subfamily H member 2(KCNH2)is a promising target for incretin secretagogue therapies

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signal Transduction and Targeted Therapy 2024年第9期9卷 4079-4090页

作者： Ying-Chao Yuan Hao Wang Ze-Ju Jiang Chang Liu Qi Li si-rui zhou Jin-Kui Yang Beijing Key Laboratory of Diabetes Research and Care Department of Endocrinology and MetabolismBeijing Diabetes InstituteBeijing Tongren HospitalCapital Medical UniversityBeijing 100730China Laboratory for Clinical Medicine Capital Medical UniversityBeijing 100069China Subcenter of State Key Laboratory of Kidney Disease Beijing Tongren HospitalCapital Medical UniversityBeijing 100730China

Derived from enteroendocrine cells(EECs),glucagon-like peptide-1(GLP-1)and glucose-dependent insulinotropic peptide(GIP)are pivotal incretin hormones crucial for blood glucose *** of GLP-1 analogs and GLP-1 receptor activators are extensively used in the treatment of type 2 diabetes(T2D)and ***,there are currently no agents to stimulate endogenous incretin ***,we find the pivotal role of KCNH2 potassium channels in the regulation of incretin ***-localization of KCNH2 with incretin-secreting EECs in the intestinal epithelium of rodents highlights its *** epithelial cell-specific KCNH2 knockout in mice improves glucose tolerance and increases oral glucose-triggered GLP-1 and GIP secretion,particularly ***,KCNH2-deficient primary intestinal epithelial cells exhibit heightened incretin,especially GIP secretion upon nutrient ***,KCNH2 knockdown in EECs leads to reduced K+currents,prolonged action potential duration,and elevated intracellular calcium ***,we found that dofetilide,a KCNH2-specific inhibitor,could promote incretin secretion in enteroendocrine STC-1 cells in vitro and in hyperglycemic mice in *** findings elucidate,for the first time,the mechanism and application of KCNH2 in regulating incretin secretion by *** the therapeutic promise of GLP-1 and GIP in diabetes and obesity management,this study advances our understanding of incretin regulation,paving the way for potential incretin secretagogue therapies in the treatment of diabetes and obesity.

关键词： KCNH2 Potassium elevated

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Hepatocyte growth factor promotes retinal pigment epithelium cell activity through MET/AKT signaling pathway

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International Journal of Ophthalmology(English edition) 2024年第5期17卷 806-814页

作者： si-rui zhou Yu-Sheng Zhu Wen-Ting Yuan Xiao-Yan Pan Tong Wang Xiao-Dong Chen Faculty of Life Sciences and Medicine Northwest UniversityXi’an 710069Shaanxi ProvinceChina First Affiliated Hospital of Northwest University Northwest UniversityXi’an 710069Shaanxi ProvinceChina Department of Ophthalmology Xi’an No.1 HospitalXi’an 710002Shaanxi ProvinceChina Shaanxi Institute of Ophthalmology Shaanxi Provincial Key Lab of OphthalmologyClinical Research Center for Ophthalmology Diseases of Shaanxi ProvinceXi’an 710002Shaanxi ProvinceChina

AIM:To explore the effects of hepatocyte growth factor(HGF)on retinal pigment epithelium(RPE)cell ***:The human adult retinal pigment epithelial cell line-19(ARPE-19)were treated by HGF or mesenchymalepithelial transition factor(MET)inhibitor SU11274 in *** viability was detected by a Cell Counting Kit-8 *** proliferation and motility was detected by a bromodeoxyuridine incorporation assay and a wound healing assay,*** expression levels of MET,phosphorylated MET,protein kinase B(AKT),and phosphorylated AKT proteins were determined by Western blot *** MET and phosphorylated MET proteins were also determined by immunofluorescence ***:HGF increased ARPE-19 cells’viability,proliferation and migration,and induced an increase of phosphorylated MET and phosphorylated AKT ***11274 significantly reduced cell viability,proliferation,and migration and decreased the expression of MET and AKT ***11274 suppressed HGF-induced increase of viability,proliferation,and migration in ARPE-19 ***,SU11274 also blocked HGF-induced phosphorylation of MET and AKT ***:HGF enhances cellular viability,proliferation,and migration in RPE cells through the MET/AKT signaling pathway,whereas this enhancement is suppressed by the MET inhibitor ***-induced MET/AKT signaling might be a vital contributor of RPE cells survival.

关键词： hepatocyte growth factor mesenchymal epithelial transition factor SU11274 retinal pigment epithelial cells

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羊膜移植在眼部疾病治疗中的应用研究进展

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国际眼科杂志 2023年第7期23卷 1149-1152页

作者：周思蕊陈晓冬潘小燕王彤西北大学附属第一医院、西安市第一医院中国陕西省西安市710069 西北大学中国陕西省西安市710069

羊膜是人胎盘中最内层的膜,支持上皮化,具有抗纤维化、抗炎、抗血管生成等特性,在眼科手术和其他外科手术中的应用逐渐广泛。最近几年羊膜在眼科临床的使用逐渐频繁,并且取得较好的疗效。本文就近年来羊膜移植在眼部烧伤、结膜病变、角... 详细信息

羊膜是人胎盘中最内层的膜,支持上皮化,具有抗纤维化、抗炎、抗血管生成等特性,在眼科手术和其他外科手术中的应用逐渐广泛。最近几年羊膜在眼科临床的使用逐渐频繁,并且取得较好的疗效。本文就近年来羊膜移植在眼部烧伤、结膜病变、角膜病变、翼状胬肉、青光眼以及黄斑裂孔等眼部疾病中的应用进展进行综述,期待羊膜移植为眼部疾病治疗带来新的思路。

关键词：羊膜羊膜移植眼部疾病研究进展

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70℃干热处理德兰臭草种子内生真菌的研究

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草地学报 2016年第6期24卷 1318-1322页

作者：施宠鲁益黄长福张蕊思安沙舟新疆农业大学草业与环境科学学院新疆草地资源与生态重点实验室新疆乌鲁木齐830052

为了更好地研究内生真菌与德兰臭草(Melica transsilvanica)的互作关系,需获得内生真菌种群已失活的德兰臭草种子。本试验采用70℃干热的方法对德兰臭草带菌种子分别处理5d,10d,15d,20d,25d和30d,以不作处理的种子为对照,通过测定各处... 详细信息

为了更好地研究内生真菌与德兰臭草(Melica transsilvanica)的互作关系,需获得内生真菌种群已失活的德兰臭草种子。本试验采用70℃干热的方法对德兰臭草带菌种子分别处理5d,10d,15d,20d,25d和30d,以不作处理的种子为对照,通过测定各处理后种子的萌发指标、生长指标和植株内生真菌数量等综合评价内生真菌的灭活效果。结果表明,70℃干热处理能有效杀灭德兰臭草种子中的内生真菌;70℃处理5d后,与对照相比,宿主染菌率下降为50%;处理25d时,种子和植株中均未分离出内生真菌。同时,70℃处理25d时,种子发芽率、发芽势、发芽指数、植株鲜重和胚根、胚芽长度以及植株株高、叶片数、分蘖数、叶宽和茎粗与对照相比均没有显著影响。值得一提的是,70℃干热处理种子可促进植株须根数的增多和根长的增加。综合比较得出,70°C干热处理25d能使德兰臭草种子中全部内生真菌失活,是一种简单、方便、可行的方法。

关键词：德兰臭草内生真菌高温处理效果评价

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光相干断层扫描血管成像观察新生血管性ARMD的临床研究

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国际眼科杂志 2018年第12期18卷 2248-2252页

作者：闵晓雪周思睿牟佳刘依琳张明四川大学华西医院眼科中国四川省成都市610041

目的:利用光相干断层扫描血管成像(optical coherence tomography angiography,OCTA)观察新生血管性年龄相关性黄斑变性(neovascular age-related macular degeneration,n ARMD)患者的脉络膜新生血管(choroidal neovascularization,CNV)及接受抗血管内皮生长因子(vascular endothelial growth factor,VEGF)治疗前后的变化。方法:本研究为病例系列分析研究。纳入2017-05/12就诊于我院眼科的n ARMD患者29例37眼。所有患者玻璃体腔注射抗VEGF治疗前和治疗后1d,1wk,1mo及每月随访时均行OCTA检查,共随访3～6mo,观察抗VEGF治疗前后CNV病灶形态和大小、中心凹旁浅层视网膜血管密度和血流灌注的变化。结果:n ARMD患者CNV病灶的组织结构中不成熟的结构、小分支血管和毛细血管对抗VEGF的治疗应答反应较好;术前基线病灶面积为1. 27±1. 88mm2,术后第1d病灶面积为1. 13±1. 79mm2,CNV病灶在抗VEGF治疗后1d即可缩小,最终病灶大小稳定在1mo时的病灶面积水平,与治疗前比较差异有统计学意义(P=0. 001);抗VEGF治疗后3mo,中心凹旁浅层视网膜血管密度和血流灌注明显降低,差异有统计学意义(P=0. 003、0. 015)。结论:OCTA能够无创、清晰地显示n ARMD患者CNV病灶的细微结构变化和定量分析CNV病灶面积的变化。OCTA还能够对视网膜血管进行分层显示,定量分析视网膜微循环的变化,在nARMD患者的病情监测和指导治疗方面有重要的临床应用价值。

关键词：光相干断层扫描血管成像新生血管性年龄相关性黄斑变性血管内皮生长因子脉络膜新生血管

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Postnatal development of rat retina:a continuous observation and comparison between the organotypic retinal explant model and in vivo development

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Neural Regeneration Research 2025年第3期20卷 900-912页

作者： Baoqi Hu rui Wang Hanyue Zhang Xiou Wang sijia zhou Bo Ma Yan Luan Xin Wang Xinlin Chen Zhichao Zhang Qianyan Kang Department of Ophthalmology The First Affiliated Hospital of Xi’an Jiaotong UniversityXi’anShaanxi ProvinceChina Department of Ophthalmology The First Affiliated Hospital of Northwest UniversityXi’anShaanxi ProvinceChina Institute of Neurobiology Xi’an Jiaotong University Health Science CenterXi’anShaanxi ProvinceChina

The organotypic retinal explant culture has been established for more than a decade and offers a range of unique advantages compared with in vivo experiments and cell ***,the lack of systematic and continuous comparison between in vivo retinal development and the organotypic retinal explant culture makes this model controversial in postnatal retinal development ***,we aimed to verify the feasibility of using this model for postnatal retinal development studies by comparing it with the in vivo *** this study,we showed that postnatal retinal explants undergo normal development,and exhibit a consistent structure and timeline with retinas in ***,we used SOX2 and PAX6 immunostaining to identify retinal progenitor *** then examined cell proliferation and migration by immunostaining with Ki-67 and doublecortin,***-67-and doublecortin-positive cells decreased in both in vivo and explants during postnatal retinogenesis,and exhibited a high degree of similarity in abundance and distribution between ***,we used Ceh-10 homeodomain-containing homolog,glutamate-ammonia ligase(glutamine synthetase),neuronal nuclei,and ionized calcium-binding adapter molecule 1 immunostaining to examine the emergence of bipolar cells,Müller glia,mature neurons,and microglia,*** timing and spatial patterns of the emergence of these cell types were remarkably consistent between in vivo and explant *** study showed that the organotypic retinal explant culture model had a high degree of consistency with the progression of in vivo early postnatal retina *** findings confirm the accuracy and credibility of this model and support its use for long-term,systematic,and continuous observation.

关键词： bipolar cells differentiation in vivo microglia Müller glia organotypic retinal explant culture postnatal retina development proliferation retinal progenitor cells

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Interfacial Zn2+-solvation regulator towards reversible and stable Zn anode

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Journal of Energy Chemistry 2025年第1期100卷 684-692页

作者： Miao zhou Xiongbin Luo Hang Li Shan Guo Zhuang Tong Xiaotao zhou Xu Li Zhaohui Hou Shuquan Liang Guozhao Fang College of Mechanical Engineering Key Laboratory of Hunan Province for Advanced Carbon-based Functional Materials Hunan Institute of Science and Technology School of Materials Science and Engineering Key Laboratory of Electronic Packaging and Advanced Functional Materials of Hunan Province Central South University College of Materials and Metallurgy Guizhou University National Energy Metal Resources and New Materials Key Laboratory Central South University

Aqueous zinc-ion batteries （AZIBs） are fundamentally challenged by the instability of the electrode/electrolyte interface,predominantly due to irreversible zinc （Zn） deposition and hydrogen ***,the intricate mechanisms behind the electrochemical discrepancies induced by interfacial Zn2+-solvation and deposition behavior demand comprehensive *** molecules endowed with special functional groups （such as hydroxyl,carboxyl,etc.） have the potential to significantly optimize the solvation structure of Zn2+and regulate the interfacial electric double layer （EDL）.By increasing nucleation overpotential and decreasing interfacial free energy,these functional groups facilitate a lower critical nucleation radius,thereby forming an asymptotic nucleation model to promote uniform Zn ***,this study presents a pioneering approach by introducing trace amounts of n-butanol as solvation regulators to engineer the homogenized Zn （H-Zn） anode with a uniform and dense *** interfacial reaction and structure evolution are explored by in/ex-situ experimental techniques,indicating that the H-Zn anode exhibits dendrite-free growth,no by-products,and weak hydrogen evolution,in sharp contrast to the bare ***,the H-Zn anode achieves a remarkable Zn utilization rate of approximately 20% and simultaneously sustains a prolonged cycle life exceeding 500 ***,the H-Zn//NH4V4O10（NVO） full battery showcases exceptional cycle stability,retaining 95.04%capacity retention after 400 cycles at a large current density of 5 A *** study enlightens solvation-regulated additives to develop Zn anode with superior utilization efficiency and extended operational lifespan.

关键词： Aqueous zinc-ion batteries Interfacial reaction Asymptotic nucleation model Reversible and stable Zn anode

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A low redox potential and long life organic anode material for sodium-ion batteries

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Journal of Energy Chemistry 2025年第1期100卷 557-564页

作者： Zhi Li Yang Wei Kang zhou Xin Huang Xing zhou Jie Xu Taoyi Kong Junwei Lucas Bao Xiaoli Dong Yonggang Wang Department of Chemistry and Shanghai Key Laboratory of Molecular Catalysis and Innovative Materials Institute of New Energy Collaborative Innovation Center of Chemistry for Energy Materials (iChEM) Fudan University Department of Chemistry Boston College School of Materials Science and Engineering Anhui University of Technology

Sodium-ion batteries （siBs） with organic electrodes are an emerging research direction due to the sustainability of organic materials based on elements like C,H,O,and sodium ***,organic electrode materials for siBs are mainly used as cathodes because of their relatively high redox potentials（>1 V）.Organic electrodes with low redox potential that can be used as anode are ***,a novel organic anode material （tetrasodium 1,4,5,8-naphthalenetetracarboxylate,Na4TDC） has been developed with low redox potential （situ/ex-situ spectroscopy and theoretical calculations,showing a high capacity of 208 mAh/g and an average decay rate of merely 0.022%per ***,the Na4TDC-hard carbon composite can further acquire improved capacity and cycling stability for 1200 cycles even with a high mass loading of up to 20 mg *** pairing with a thick Na3V2（PO4）3cathode (20.6 mg cm-2),the as-fabricated full cell exhibited high operating voltage （2.8 V）,excellent rate performance and cycling stability with a high capacity retention of 88.7% after 200 cycles,well highlighting the Na4TDC anode material for siBs.

关键词： Organic anode material Low redox potential Composite anode Sodium-ion batteries

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Fine-tuning the spatial allocation of phytohormones for smart canopy, smart breeding, and smart agriculture

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Science China Life Sciences 2025年第1期 309-310页

作者： Tao zhou Lin Li National Key Laboratory of Crop Genetic Improvement Huazhong Agricultural University HuBei HongShan Laboratory

The dual challenges of global climate change and population growth have increased demand for higher crop yields per unit area. The innovative technology of smart agriculture aims to synergize breeding, cultivation, and production through the application of biological and informatics technologies. Historically, the “Green Revolution” introduced semidwarf varieties of certain key crops by utilizing mutations affecting gibberellin pathways, which significantly increased planting densities and yields.

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