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Arbuscular mycorrhizal fungi differ in affecting the flowering of a host plant under two soil phosphorus conditions

Journal of Plant Ecology 2018年第4期11卷 623-631页

作者： Shijun Liu Hanling Guo Jing Xu Zeyuan song shurui song Jianjun Tang Xin Chen Department of Ecology College of Life SciencesZhejiang UniversityNo.866 Yuhangtang RoadHangzhou 310058China

Aims Studies have showed that arbuscular mycorrhizal fungi(AMF)can greatly promote the growth of host plants,but how AMF affect flow-ering phenology of host plants is not well ***,we con-ducted a pot experiment to test whether life cycle and flowering phenology traits of host plant Medicago truncatula Gaertn can be altered by AMF under low and high soil phosphorus(P)*** the experiment was conducted in a greenhouse at Zhejiang University in China(120°19′E,30°26′N)and had a completely ran-domized design with two factors:AMF treatments and soil P *** AMF species(Acaulospora scrobiculata,As;Gigaspora marga-rita,Gma;Funneliformis geosporum,Fg;Rhizophagus intraradices,Ri;Funneliformis mosseae,Fmo and Glomus tortuosum,Gt.)were used,and two soil P levels(24.0 and 5.7 mg kg−1 Olsen-soluble P)were *** six AMF species were separately inoculated or in a mixture(Mix),and a non-AMF control(NAMF)was *** plants began to flower,the number of flowers in each pot was recorded *** fruit ripening,the number of mature fruits was also recorded ***~4 months,the biomass,bio-mass P content and AMF colonization of host plant were *** between root colonization and first flowering time,or P content and first flowering time was *** Findings Under the low P level,first flowering time negatively correlated with root colonization and biomass *** host plants with AMF spe-cies As,Fg,Ri,or Mix were able to complete their life cycle within 112 days after *** treatment with AMF species Fg,Gt,or As resulted in two periods of rapid flower production while other fungi treatments resulted in only one within 112 days after *** cumulative number of flowers produced and biomass P content were highest with species *** biomass allocation significantly differed depending on the species of *** both soil P levels,the host plant tended to allocate more biomass to fruits in the Mix treatment than in

关键词： biomass allocation flowering phenology life cycle Medicago truncatula Gaertn Mycorrhizae

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The LpHsfA2-molecular module confers thermotolerance via fine tuning of its transcription in perennial ryegrass(Lolium perenne L.)

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Journal of Integrative Plant Biology 2024年第11期66卷 2346-2361页

作者： Guangjing Ma Zhihao Liu shurui song Jing Gao Shujie Liao Shilong Cao Yan Xie Liwen Cao Longxing Hu Haichun Jing Liang Chen CAS Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture Wuhan Botanical GardenChinese Academy of SciencesWuhan 430074China University of Chinese Academy of Sciences Beijing 100049China Key Laboratory of Edible Wild Plants Conservation and Utilization Hubei Normal UniversityHuangshi 435002China Department of Pratacultural Sciences College of AgronomyHunan Agricultural UniversityChangsha 410128China Key Laboratory of Plant Resources Institute of BotanyChinese Academy of SciencesBeijing 100093China Academician Workstation of Agricultural High-tech Industrial Area of the Yellow River Delta National Center of Technology Innovation for Comprehensive Utilization of Saline-Alkali LandDongying 257300China

Temperature sensitivity and tolerance play a key role in plant survival and *** ryegrass(Lolium perenne L.),widely cultivated in cool-season for forage supply and turfgrass,is extremely susceptible to high temperatures,therefore serving as an excellent grass for dissecting the genomic and genetic basis of high-temperature *** this study,expression analysis revealed that LpHsfA2,an important gene associated with high-temperature tolerance in perennial ryegrass,is rapidly and substantially induced under heat ***,heat-tolerant varieties consistently display elevated expression levels of LpHsfA2 compared with heat-sensitive *** haplotype analysis of the LpHsfA2 promoter indicated an uneven distribution of two haplotypes(HsfA2^(Hap1) and HsfA2^(Hap2)) across varieties with differing heat ***,the HsfA2^(Hap1) allele is predominantly present in heat-tolerant varieties,while the HsfA2^(Hap2) allele exhibits the opposite *** of LpHsfA2 confers enhanced thermotolerance,whereas silencing of LpHsfA2 compromises heat ***,LpHsfA2 orchestrates its protective effects by directly binding to the promoters of LpHSP18.2 and LpAPX1 to activate their expression,preventing the non-specific misfolding of intracellular protein and the accumulation of reactive oxygen species in ***,LpHsf A4 and LpHsf A5 were shown to engage directly with the promoter of LpHsfA2,upregulating its expression as well as the expression of LpHSP18.2 and LpAPX1,thus contributing to enhanced heat ***,LpHsfA2 possesses autoregulatory ability by directly binding to its own promoter to modulate the *** on these findings,we propose a model for modulating the thermotolerance of perennial ryegrass by precisely regulating the expression of ***,these findings provide a scientific basis for the development of thermotolerant perennial ryegrass culti

关键词： haplotype heat shock transcription factors heat tolerance perennial ryegrass

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Genome-wide identification of the CONSTANS-LIKE(COL)family and mechanism of fruit senescence regulation by PpCOL8 in sand pear(Pyrus pyrifolia)

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Journal of Integrative Agriculture 2024年第4期23卷 1222-1237页

作者： Yue Xu shurui song Huiying Wang Xilong Cao Xinran Zhao Wenli Wang Liyue Huo Yawei Li Misganaw Wassie Bin Lu Liang Chen Haiyan Shi College of Horticulture Hebei Agricultural UniversityBaoding 071001China Wuhan Botanical Garden Chinese Academy of SciencesWuhan 430074China

Pyrus pyrifolia Nakai‘Whangkeumbae'is a sand pear fruit with excellent nutritional quality and ***,the industrial development of pear fruit is significantly limited by its short shelf *** acid(SA),a well-known phytohormone,can delay fruit senescence and improve shelf ***,the mechanism by which SA regulates CONSTANS-LIKE genes(COLs)during fruit senescence and the role of COL genes in mediating fruit senescence in sand pear are poorly *** this study,22 COL genes were identified in sand pear,including four COLs(Pp COL8,Pp COL9a,Pp COL9b,and Pp COL14)identified via transcriptome analysis and 18 COLs through genome-wide *** COL genes were divided into three subgroups according to the structural domains of the COL *** COL8,with two B-box motifs and one CCT domain,belonged to the first *** contrast,the other three Pp COLs,Pp COL9a,Pp COL9b,and Pp COL14,with similar conserved protein domains and gene structures,were assigned to the third *** four COLs showed different expression patterns in pear tissues and were preferentially expressed at the early stage of fruit ***,the expression of Pp COL8 was inhibited by exogenous SA treatment,while SA up-regulated the expression of Pp COL9a and Pp ***,Pp COL8 interacts with Pp MADS,a MADS-box protein preferentially expressed in fruit,and SA up-regulated its *** the production of ethylene and the content of malondialdehyde(MDA)were increased in Pp COL8-overexpression sand pear fruit,the antioxidant enzyme(POD and SOD)activity and the expression of Pp POD1 and Pp SOD1 in the sand pear fruits were down-regulated,which showed that Pp COL8 promoted sand pear fruit *** contrast,the corresponding changes were the opposite in Pp MADS-overexpression sand pear fruits,suggesting that Pp MADS delayed sand pear fruit *** co-transformation of Pp COL8 and Pp MADS also delayed sand pear fruit *** re

关键词： Pyrus pyrifolia CONSTANS-LIKE gene salicylic acid fruit senescence MADS

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浅谈馆藏档案开放鉴定工作三步骤

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档案管理 2021年第5期 78-79页

作者：宋淑睿河南省档案馆

本文以档案鉴定工作为研究对象,从工作实际出发阐述了鉴定前、鉴定中和鉴定后三个阶段的工作内容和工作特点和难点,总结经验,指导实际工作。

关键词：档案开放档案鉴定终审档案数据统计利用

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不同钾肥对渍水胁迫下紫花苜蓿叶绿素荧光特性的影响

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草业科学 2021年第4期38卷 693-702页

作者：宋述锐于华黄椰张卫红中国科学院植物种质创新与特色农业重点实验室中国科学院武汉植物园湖北武汉430074 中国科学院大学北京100049

渍水胁迫是我国易涝地区限制紫花苜蓿(Medicago sativa)生产的一个重要非生物胁迫因素,如何有效提高紫花苜蓿的耐渍水能力是当前亟需解决的重要问题。本研究采用盆栽试验法,通过模拟渍水试验比较了渍水胁迫下施用不同钾肥对紫花苜蓿叶... 详细信息

渍水胁迫是我国易涝地区限制紫花苜蓿(Medicago sativa)生产的一个重要非生物胁迫因素,如何有效提高紫花苜蓿的耐渍水能力是当前亟需解决的重要问题。本研究采用盆栽试验法,通过模拟渍水试验比较了渍水胁迫下施用不同钾肥对紫花苜蓿叶绿素含量、叶绿素a荧光和慢速荧光的影响。研究结果表明,渍水胁迫下,施用5 mmol·L^(−1) KCl和5 mmol·L^(−1) KNO_(3)能够显著提高紫花苜蓿的叶绿素含量(P<0.05),减少光合元件损伤,显著提高了叶绿素a荧光最大光合效率和实际光合效率,从而有效促进了紫花苜蓿的生长和生物量的积累,且KNO_(3)对紫花苜蓿耐渍水能力的提高作用优于KCl。因此,施用钾肥能够提高紫花苜蓿的耐渍水能力。

关键词：紫花苜蓿耐涝性氯化钾硝酸钾生长特性光合效率叶绿素

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Special RCA based sensitive point-of-care detection of HPV mRNA for cervical cancer screening

Aggregate

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Aggregate 2024年第4期5卷 399-415页

作者： Yi Long shurui Tao Dongni Shi Xingyu Jiang Tian Yu Yingxi Long Libing song Guozhen Liu Integrated Devices and Intelligent Diagnosis(ID2)Laboratory CUHK(SZ)-Boyalife Joint Laboratory for Regenerative Medicine EngineeringSchool of MedicineThe Chinese University of Hong KongShenzhenChina Ciechanover Institute of Precision and Regenerative Medicine School of MedicineThe Chinese University of Hong KongShenzhenChina Department of Experimental Research Sun Yat-sen University Cancer CenterState Key Laboratory of Oncology in South ChinaCollaborative Innovation Center for Cancer MedicineGuangzhouChina

Developing the sensitive point-of-care testing(POCT)of oncogenic nucleic acids from human papillomavirus(HPV)infection is essential in preventing cervical can-cer,especially in resource-limited *** circle ampliﬁcation(RCA)is attractive in achieving POCT via nucleic acid-based aggregation under isothermal ***,the inﬂuence of RCA product structure on the aggregation remains unexplored resulting in limited ***,a minimum secondary structured RCA technique(MSS-RCA)is developed by designing a unique circu-lar template,demonstrating signiﬁcantly enhanced detection sensitivity with only one ampliﬁcation step and one primer under isothermal *** ampliﬁ-cation efﬁciency of MSS-RCA could be kinetically manipulated by controlling the secondary structure of the circular *** the invertase probe to MSS-RCA,HPV16 E6/E7 nucleic acid target was detected with a personal glucose meter(PGM)with a sensitivity of 5 fM(50 zmol in 10µL).This integrated MSS-RCA-PGM detection system was successfully applied to detect HPV16 E6/E7 mRNA extracted from 54 cervical swab samples reaching a positive predictive value of 100.00%and negative predictive values of 96.00%(77.77%to 99.40%,95%CI).MSS-RCA-PGM provides a sensitive POCT platform for the detection of nucleic acid biomarkers for screening of cervical cancer or other diseases.

关键词： cervical cancer screening HPV mRNA nucleic acid-based aggregation point-of-care detection rolling circle ampliﬁcation sensitivity

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Radiation-hardened property of single-walled carbon nanotube film-based field-effect transistors under low-energy proton irradiation

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Journal of Semiconductors 2021年第11期42卷 18-25页

作者： Xiaorui Zhang Huiping Zhu song’ang Peng Guodong Xiong Chaoyi Zhu Xinnan Huang shurui Cao Junjun Zhang Yunpeng Yan Yao Yao Dayong Zhang Jingyuan Shi Lei Wang Bo Li Zhi Jin High-Frequency High-Voltage Device and Integrated Circuits R&D Center Institute of MicroelectronicsChinese Academy of SciencesBeijing 100029China Key Laboratory of Science and Technology on Silicon Devices Institute of MicroelectronicsChinese Academy of SciencesBeijing 100029China University of Chinese Academy of Sciences Beijing 100049China Department of Chemistry City University of Hong KongHong Kong 999077China

Strong C-C bonds,nanoscale cross-section and low atomic number make single-walled carbon nanotubes(SWCNTs)a potential candidate material for integrated circuits(ICs)applied in outer ***,very little work combines the simulation calculations with the electrical measurements of SWCNT field-effect transistors(FETs),which limits further understanding on the mechanisms of radiation ***,SWCNT film-based FETs were fabricated to explore the total ionizing dose(TID)and displacement damage effect on the electrical performance under low-energy proton irradiation with different fluences up to 1×1015 p/*** negative shift of the threshold voltage and obvious decrease of the on-state current verified the TID effect caused in the oxide *** stability of the subthreshold swing and the off-state current reveals that the displacement damage caused in the CNT layer is not serious,which proves that the CNT film is ***,according to the simulation,we found the displacement damage caused by protons is different in the source/drain contact area and channel area,leading to varying degrees of change for the contact resistance and sheet *** analyzed the simulation results and electrical measurements,we explained the low-energy proton irradiation mechanism of the CNT FETs,which is essential for the construction of radiation-hardened CNT film-based ICs for aircrafts.

关键词： SWCNT FETs low-energy proton irradiation radiation effects electrical performance TID effect displacement damage effect simulation

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管理者短视影响审计收费吗?——来自上市公司的经验证据

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审计研究 2024年第4期 89-101页

作者：许宁宁王书睿宋佳莹天津理工大学管理学院 300384

在可持续发展战略导向下,管理者短视在对企业决策产生不利影响的同时,如何影响审计费用值得关注。研究发现,管理者短视程度越高,审计费用越高,经营风险、控制风险以及盈余管理在管理者短视对审计费用的影响中发挥了中介作用。进一步研... 详细信息

在可持续发展战略导向下,管理者短视在对企业决策产生不利影响的同时,如何影响审计费用值得关注。研究发现,管理者短视程度越高,审计费用越高,经营风险、控制风险以及盈余管理在管理者短视对审计费用的影响中发挥了中介作用。进一步研究发现,董事长与总经理间权力距离较大时,管理者短视对审计费用的影响更显著,而较高的监督型机构投资者持股比例可以抑制管理者短视与审计费用间的正向关系。拓展分析发现,管理者短视程度越高,审计报告中关键审计事项披露数量越多,且高声誉会计师事务所出具非标审计意见的概率越高。研究将管理者短视产生的影响拓展至审计领域,对企业高管聘任和事务所优化风险评估程序均有启示意义。

关键词：管理者短视审计风险审计费用

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基于文本数据的过滤式与嵌入式样本选择算法

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数据分析与知识发现 2020年第2期4卷 223-230页

作者：刘书瑞田继东陈普春赖立宋国杰西南石油大学理学院成都610500

【目的】减少文本数据的训练数据量,缩短模型训练时间。【方法】基于协方差估计,提出一种新的过滤式样本选择算法,并将数据的遗忘性研究成果应用到嵌入式样本选择算法中。【结果】在中文阅读理解模型训练中,本文提出的算法至少可以减少... 详细信息

【目的】减少文本数据的训练数据量,缩短模型训练时间。【方法】基于协方差估计,提出一种新的过滤式样本选择算法,并将数据的遗忘性研究成果应用到嵌入式样本选择算法中。【结果】在中文阅读理解模型训练中,本文提出的算法至少可以减少模型训练时间50%。与经典的词频-逆文档频次算法相比,本文小批量协方差估计算法与遗忘算法在召回率、F评价指标上分别提升0.018、0.012与0.017、0.029。【局限】训练数据减少,对模型的准确率评价指标有一定影响。【结论】本文算法能减少模型的训练时间,提高评价指标,由于计算只与批次有关,故适用于大规模数据集的并行运算。

关键词：样本选择协方差估计遗忘算法

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联合面部线索与眼动特征的在线学习专注度识别

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中国电化教育 2022年第11期 37-44页

作者：武法提赖松高姝睿李鲁越任伟祎北京师范大学教育学部教育技术学院北京100875 北京师范大学数字学习与教育公共服务教育部工程研究中心北京100875

专注是产生有效学习的先决条件,是取得良好学习成效的重要保证,在以自主学习为主的在线学习场景中具有更为重要的作用,但在线学习时空分离的特性难以保证学习者的专注度得到及时监控,故而探究精准识别在线学习专注度的可行方法至关重要... 详细信息

专注是产生有效学习的先决条件,是取得良好学习成效的重要保证,在以自主学习为主的在线学习场景中具有更为重要的作用,但在线学习时空分离的特性难以保证学习者的专注度得到及时监控,故而探究精准识别在线学习专注度的可行方法至关重要。该研究主要关注在线学习中学习者的面部线索与眼动特征,基于从视频数据中提取的眼部视线、头部姿态、面部动作单元等面部线索特征以及从眼动数据中提取的注视停留时间、注视点、眼跳等眼动特征,分别通过两类单模态特征以及联合二者的多模态特征进行学习专注度识别,采用常用的六种机器学习方法构建相应的评估模型,对六种分类器的专注度预测性能进行了比较,并判断了专注度与学习成效的关系。实验结果表明,相较于面部线索,眼动特征具有更好的识别潜力,其体现的信息加工机制更能反映心理资源的投入程度;与单一模态相比,模态融合能显著提高学习专注度识别效果,揭示了面部线索特征和眼动特征对学习专注度识别的互补性;而学习专注度与学习成效显著相关,故而可将专注度作为优化在线学习的主要抓手,从学习材料设计者、教师与学习者等不同主体出发改善在线学习过程,提升在线学习效果。

关键词：学习专注度眼动面部线索学习分析多模态数据

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