检索结果-南通市图书馆

Streamlined human antibody generation and optimization by exploiting designed immunoglobulin loci in a B cell line

Cellular & Molecular Immunology 2021年第6期18卷 1545-1561页

作者： Hidetaka Seo Hitomi Masuda Kenjiro Asagoshi Tomoaki Uchiki Shigehisa Kawata Goh Sasaki Takashi Yabuki shunsuke miyai Naoki Takahashi Shu-ichi Hashimoto Atsushi Sawada Aki Takaiwa Chika Koyama Kanako Tamai Kohei Kurosawa Ke-Yi Lin Kunihiro Ohta Yukoh Nakazaki Department of Life Sciences Graduate School of Arts and SciencesThe University of TokyoTokyoJapan Research Laboratories Chiome Bioscience IncTokyoJapan

Monoclonal antibodies(mAbs)are widely utilized as therapeutic drugs for various diseases,such as cancer,autoimmune diseases,and infectious diseases.Using the avian-derived B cell line DT40,we previously developed an antibody display technology,namely,the ADLib system,which rapidly generates antigen-specific mAbs.Here,we report the development of a human version of the ADLib system and showcase the streamlined generation and optimization of functional human mAbs.Tailored libraries were first constructed by replacing endogenous immunoglobulin genes with designed human counterparts.From these libraries,clones producing full-length human IgGs against distinct antigens can be isolated,as exemplified by the selection of antagonistic mAbs.Taking advantage of avian biology,effective affinity maturation was achieved in a straightforward manner by seamless diversification of the parental clones into secondary libraries followed by single-cell sorting,quickly affording mAbs with improved affinities and functionalities.Collectively,we demonstrate that the human ADLib system could serve as an integrative platform with unique diversity for rapid de novo generation and optimization of therapeutic or diagnostic antibody leads.Furthermore,our results suggest that libraries can be constructed by introducing exogenous genes into DT40 cells,indicating that the ADLib system has the potential to be applied for the rapid and effective directed evolution and optimization of proteins in various fields beyond biomedicine.

关键词： Immunoglobulin rearrangements homologous recombination antibody therapeutics antibody engineering drug discovery

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Twisted fiber microfluidics:a cutting-edge approach to 3D spiral devices

引用

Microsystems & Nanoengineering 2024年第1期10卷 193-202页

作者： shunsuke Kato Daniel W.Carlson Amy Q.Shen Yuanyuan Guo Department of Electrical Information and Physics EngineeringSchool of EngineeringTohoku UniversityAoba-kuSendai 980-8579 MiyagiJapan Micro/Bio/Nanofluidics Unit Okinawa Institute of Science and TechnologyOnnaKunigami-gun 904-0495 OkinawaJapan Frontier Research Institute for Interdisciplinary Sciences(FRIS) Tohoku UniversityAoba-kuSendai 980-0845 MiyagiJapan Graduate School of Biomedical Engineering Tohoku UniversityAoba-kuSendai 980-8579 MiyagiJapan Department of Physiology Graduate School of MedicineTohoku UniversityAoba-kuSendai 980-8575 MiyagiJapan

The development of 3D spiral microfluidics has opened new avenues for leveraging inertial focusing to analyze small fluid volumes,thereby advancing research across chemical,physical,and biological disciplines.While traditional straight microchannels rely solely on inertial lift forces,the novel spiral geometry generates Dean drag forces,eliminating the necessity for external fields in fluid manipulation.Nevertheless,fabricating 3D spiral microfluidics remains a labor-intensive and costly endeavor,hindering its widespread adoption.Moreover,conventional lithographic methods primarily yield 2D planar devices,thereby limiting the selection of materials and geometrical configurations.To address these challenges,this work introduces a streamlined fabrication method for 3D spiral microfluidic devices,employing rotational force within a miniaturized thermal drawing process,termed as mini-rTDP.This innovation allows for rapid prototyping of twisted fiber-based microfluidics featuring versatility in material selection and heightened geometric intricacy.To validate the performance of these devices,we combined computational modeling with microtomographic particle image velocimetry(uTPiM)to comprehensively characterize the 3D flow dynamics.Our results corroborate the presence of a steady secondary flow,underscoring the effectiveness of our approach.Our 3D spiral microfluidics platform paves the way for exploring intricate microflow dynamics,with promising applications in areas such as drug delivery,diagnostics,and lab-on-a-chip systems.

关键词： thereby straight twisted

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

From text to image:challenges in integrating vision into ChatGPT for medical image interpretation

引用

Neural Regeneration Research 2025年第2期20卷 487-488页

作者： shunsuke Koga Wei Du Department of Pathology and Laboratory Medicine Hospital of the University of PennsylvaniaPhiladelphiaPAUSA

Large language models(LLMs),such as ChatGPT developed by OpenAI,represent a significant advancement in artificial intelligence(AI),designed to understand,generate,and interpret human language by analyzing extensive text data.Their potential integration into clinical settings offers a promising avenue that could transform clinical diagnosis and decision-making processes in the future(Thirunavukarasu et al.,2023).This article aims to provide an in-depth analysis of LLMs’current and potential impact on clinical practices.Their ability to generate differential diagnosis lists underscores their potential as invaluable tools in medical practice and education(Hirosawa et al.,2023;Koga et al.,2023).

关键词： image diagnosis text

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Pulmonary artery diameter ratio as a prognostic indicator of congenital diaphragmatic hernia

引用

World Journal of Pediatric Surgery 2024年第1期7卷 20-25页

作者： Yuichiro Miyake Hiroyuki Koga Shuko Nojiri shunsuke Yamada Takanori Ochi Go Miyano Geoffrey J Lane Atsuyuki Yamataka Tadaharu Okazaki Department of Pediatric General and Urogenital Surgery Juntendo University School of MedicineBunkyo-kuTokyoJapan Department of Medical Technology Innovation Center Juntendo UniversityBunkyo-ku TokyoJapan Department of Pediatric Surgery Juntendo University Urayasu HospitalUrayasuChibaJapan

Background Following on from an earlier study published in 2008 about left pulmonary artery(LPA)flow measured on serial echocardiography being strongly prognostic in left-sided congenital diaphragmatic hernia(CDH)and the ratio of LPA to right pulmonary artery(RPA)diameters being a simple and reliable indicator for commencing nitric oxide(NO)therapy,the ratio of LPA:RPA diameters(PA ratio orPAR)was hypothesized to possibly reflect cardiopulmonary stresses accompanying CDH better.Methods Subjects with isolated left-sided CDH treated between 2007 and 2020 at a single pediatric surgical center were recruited and classified according to survival.Data obtained retrospectively for subject demographics,clinical course,LPA/RPA diameters,and PAR were compared between survivors and non-survivors.The value of PAR for optimizing the prognostic value of PA diameter data in CDH were analyzed with receiver operating characteristic(ROC)curve analysis.Results Of 65 subjects,there were 54 survivors(82.3%)and 11 non-survivors(17.7%);7 of 11 non-survivors died before surgical repair could be performed.Mean PAR for survivors(0.851±0.152)was significantly higher than for non-survivors(0.672±0.108)(p=0.0003).Mean PAR for non-survivors was not affected by surgical repair.Characteristics of survivors were:LPA≥2 mm(n=52 of 54;mean PAR=0.866±0.146)and RPA≥3mm(n=46 of 54;mean PAR=0.857±0.152).Non-survivors with similar LPA and RPA diameters to survivors had significantly lower mean PAR.ROC curve cut-off for PAR was 0.762.Subjects with high PAR(≥0.762)required high-frequency oscillatory ventilation/NO less than subjects with low PAR(<0.762)(p=0.0244 and p=0.0485,respectively)and subjects with high PAR stabilized significantly earlier than subjects with low PAR(1.71±0.68 days vs 3.20±0.87 days)(p<0.0001).Conclusions PAR would appear to be strongly correlated with clinical outcome in CDH and be useful for planning management of cardiopulmonary instability in CDH.

关键词： hernia congenital diameters

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

In situ observation of the phase transformation kinetics of bismuth during shock release

引用

Chinese Physics B 2024年第4期33卷 586-592页

作者：李江涛王倩男徐亮柳雷张航 Sota Takagi Kouhei Ichiyanagi Ryo Fukaya shunsuke Nozawa 胡建波 Laboratory for Shock Wave and Detonation Physics Research Institute of Fluid PhysicsChina Academy of Engineering PhysicsMianyang 621900China State Key Laboratory for Environment-Friendly Energy Materials Southwest University of Science and TechnologyMianyang 621010China Japan Society for the Promotion of Science Tokyo102-0083Japan Institute of Materials Structure Science High Energy Accelerator Research OrganizationIbaraki305-8572Japan

A time-resolved x-ray diffraction technique is employed to monitor the structural transformation of laser-shocked bismuth.Results reveal a retarded transformation from the shock-induced Bi-Ⅴphase to a metastable Bi-Ⅳphase during the shock release,instead of the thermodynamically stable Bi-Ⅲphase.The emergence of the metastable Bi-Ⅳphase is understood by the competitive interplay between two transformation pathways towards the Bi-Ⅳand Bi-Ⅲ,respectively.The former is more rapid than the latter because the Bi-Ⅴto B-Ⅳtransformation is driven by interaction between the closest atoms while the Bi-Ⅴto B-Ⅲtransformation requires interaction between the second-closest atoms.The nucleation time for the Bi-Ⅴto Bi-Ⅳtransformation is determined to be 5.1±0.9 ns according to a classical nucleation model.This observation demonstrates the importance of the formation of the transient metastable phases,which can change the phase transformation pathway in a dynamic process.

关键词： phase transformation time-resolved x-ray diffraction(XRD) bismuth metastable phase nonequilibrium phase diagram

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

上古汉语否定词“不”“弗”演变补论”——兼论“不”分勿切的读音来源

引用

语言学论丛 2024年第2期 96-106页

作者：户内俊介日本大学日本1568550东京

本文主要讨论上古汉语的否定词“不”与“弗”,重在利用战国秦汉时期的出土文献和传世文献,揭示上古各个时期的“不”与“弗”的分合演变:在先秦时期,“弗”是“不”加前置宾语代词“之”的合音词,然而在两汉时期,代词宾语前置现象减少... 详细信息

本文主要讨论上古汉语的否定词“不”与“弗”,重在利用战国秦汉时期的出土文献和传世文献,揭示上古各个时期的“不”与“弗”的分合演变:在先秦时期,“弗”是“不”加前置宾语代词“之”的合音词,然而在两汉时期,代词宾语前置现象减少,代词宾语“之”也逐渐式微,同时“不”“弗”的功能接近,导致“弗”字的语源渐渐被忘却,“弗”不再被视为“不+之”的合音,而是成为一个带有强调语气的单纯否定词。本文还对“不”获得分勿切一读的原因提出了自己的看法。

关键词：上古汉语否定词不弗入声叶氏周期

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Preparation and characterization of tea tree oil-β-cyclodextrin microcapsules with super-high encapsulation efficiency

引用

生物质资源与工程(英文) 2023年第3期8卷 224-234页

作者： Peifu Kong Junichi Peter Abe shunsuke Masuo Toshiharu Enomae Degree Programs in Life and Earth Sciences University of TsukubaIbaraki 305-8572Japan Faculty of Life and Environmental Sciences University of TsukubaIbaraki305-8572Japan

This study aimed to prepare tea tree oil-β-cyclodextrin microcapsules using an optimized co-precipitated method.The impact of the volume fraction of ethanol in the solvent system for microencapsulation on encapsulation efficiency was investigated and analyzed sophisticatedly.Super-high encapsulation efficiency was achieved when a 40%volume fraction of ethanol was used for the microencapsulation procedure,where the recovery yield of microcapsules and the embedding fraction of tea tree oil in microcapsules were as high as 88.3%and 94.3%,respec-tively.Additionally,considering the operation cost,including time and energy consumption,an economical preparation was validated so that it would be viable for large-scale production.Based on the results of morphological and X-ray diffraction analysis,the crystal structure appeared to differ before and after microencapsulation.The results of gas chromatography-mass spectrometry and Fourier transform infrared spectroscopy confirmed the successful formation of microcapsules.Furthermore,the antibacterial activity of the fabricated microcapsules was assessed by a simple growth inhibition test using Bacillus subtilis as the study object,and the hydrophilic property was proved by a water contact angle measurement.

关键词： β-cyclodextrin Co-precipitation Encapsulation efficiency Microcapsule Tea tree oil

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Development of Bio-cultured Artificial Muscles with High Design Flexibility

引用

Journal of Bionic Engineering 2023年第4期20卷 1635-1645页

作者： Hirono Ohashi shunsuke Shigaki Ryo Teramae Masahiro Shimizu Koh Hosoda Department of Systems Innovation Osaka University1-2MachikaneyamaToyonakaOsaka560-0043Japan

Recent advances in bionics have made it possible to create various tissue and organs.Using this cell culture technology,engineers have developed a robot driven by three-dimensional cultured muscle cells(bioactuator)—a muscle cell robot.For more applications,researchers have been developed various tissues and organs with bio3D printer.However,three-dimensional cultured muscle cells printed by bio3D printer have been not used for muscle cell robot yet.The aim of our study is to develop easy fabrication method of bioactuator having high design flexibility like as bio3D printer.We fabricated three-dimensional cultured muscle cells using mold and dish having pin which can contribute to shape and cell alignment.In this study,we observed that our method maintained the shape of three-dimensional cultured muscle cells and caused cell alignment which is important for bioactuator development.We named three-dimensional cultured muscle cells developed in this study“bio-cultured artificial muscle(BiCAM)”.Finally,we observed that BiCAM contracted in response to electrical stimulus.From these data,we concluded our proposed method is easy fabrication method of bioactuator having high design flexibility.

关键词： Three-dimensional cultured muscle cells Bio-cultured artificial muscle Skeletal muscle cell Tissue engineering

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Pitfalls and promises of bile duct alternatives:A narrative review

引用

World Journal of Gastroenterology 2022年第39期28卷 5707-5722页

作者： Mitsuo Miyazawa Masayasu Aikawa Junpei Takashima Hirotoshi Kobayashi shunsuke Ohnishi Yoshito Ikada Department of Surgery Teikyo University Mizonokuch HospitalKanagawa 213-8507Japan Department of Surgery Saitama Medical University International Medical CenterSaitama 350-1298Japan Department of Gastroenterology and Hepatology Hokkaido University Faculty of Medicine and Graduate School of MedicineSapporo 060-8638Japan Department of Bioenvironmental Medicine Nara Medical UniversityNara 634-8521Japan

Biliodigestive anastomosis between the extrahepatic bile duct and the intestine for bile duct disease is a gastrointestinal reconstruction that abolishes duodenal papilla function and frequently causes retrograde cholangitis.This chronic inflammation can cause liver dysfunction,liver abscess,and even bile duct cancer.Although research has been conducted for over 100 years to directly repair bile duct defects with alternatives,no bile duct substitute(BDS)has been developed.This narrative review confirms our understanding of why bile duct alternatives have not been developed and explains the clinical applicability of BDSs in the near future.We searched the PubMed electronic database to identify studies conducted to develop BDSs until December 2021 and identified studies in English.Two independent reviewers reviewed studies on large animals with 8 or more cases.Four types of BDSs prevail:Autologous tissue,non-bioabsorbable material,bioabsorbable material,and others(decellularized tissue,3D-printed structures,etc.).In most studies,BDSs failed due to obstruction of the lumen or stenosis of the anastomosis with the native bile duct.BDS has not been developed primarily because control of bile duct wound healing and regeneration has not been elucidated.A BDS expected to be clinically applied in the near future incorporates a bioabsorbable material that allows for regeneration of the bile duct outside the BDS.

关键词： Bile duct alternative Bile duct substitute Biliary regeneration Bile duct reconstruction Peribiliary gland Bioabsorbable polymer

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Synchronous multiple lung cancers with hilar lymph node metastasis of small cell carcinoma:A case report

引用

世界临床病例杂志 2023年第25期11卷 5919-5925页

作者： Ryusei Yoshino Nana Yoshida shunsuke Yasuda Akane Ito Masaki Nakatsubo Sayaka Yuzawa Masahiro Kitada Thoracic Surgery and Breast Surgery Asahikawa Medical UniversityAsahikawa 078-8510Japan Diagnostic Pathology Asahikawa Medical University HospitalHokkaido 078-8510Japan

BACKGROUND Synchronous multiple lung cancers are rare and refer to the simultaneous presence of two or more primary lung tumors,which present significant challenges in terms of diagnosis and treatment.CASE SUMMARY We report a case of multiple synchronous lung cancers with hilar lymph node metastasis of small cell carcinoma of unknown origin in a 73-year-old man.Transbronchial lung biopsy revealed squamous cell carcinoma.Although enlargement of lymph node 12u was detected,no distant metastases were observed.The patient was preoperatively diagnosed with T1cN0M0 and underwent thoracoscopic right upper lobectomy with nodal dissection(ND2a).Based on histopathological findings,the primary lesion was squamous cell carcinoma.A microinvasive adenocarcinoma was also observed on the cranial side of the primary lesion.Tumors were detected in two resected lymph nodes(#12u and#11s).Both tumors were pathologically diagnosed as small cell carcinomas.The primary lesion of the small cell carcinoma could not be identified even by whole-body imaging;however,chemotherapy was initiated for hilar lymph node metastasis of the small cell carcinoma of unknown origin.CONCLUSION Multiple synchronous lung cancers can be accompanied by hilar lymph node metastasis of small cell carcinomas of unknown origin.

关键词： Small cell carcinoma Synchronous multiple lung cancers Squamous cell carcinoma Adenocarcinoma Chemotherapy Case report

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：