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Combining Metabolic Alkyne Labeling and Click Chemistry for Secretome Analysis of Serum-Containing Conditioned Medium

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Chinese Journal of Chemistry 2021年第7期39卷 1843-1848页

作者： Jiangnan Zheng Yuan Mao shun feng Ruijun Tian Department of Chemistry School of ScienceSouthern University of Science and TechnologyShenzhenGuangdong518055 China School of Life Science and Engineering Southwest Jiaotong UniversityChengduSichuan610031 China Guangdong Provincial Key Laboratory of Cell Microenvironment and Disease Research Southern University of Science and Technology1088 Xueyuan RoadShenzhenGuangdong518055 China

Main observation and conclusion Bioorthogonal click chemistry has emerged as a powerful tool for the specific modification of proteins in complex *** labeling of proteins with azide followed by the copper-catalyzed azide−alkyne cycloaddition(CuAAC)with alkyne-based affinity probes/beads is widely applied to study protein turnover and post-translational modifications(PTMs).

关键词： Click chemistry Mass spectrometry Proteomics Secretome Metabolic labeling

Optimal design of a novel cylindrical sandwich panel with double arrow auxetic core under air blast loading

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Defence Technology（防务技术） 2020年第3期16卷 617-626页

作者： Xu-ke Lan Qi Huang Tong Zhou shun-shan feng State Key Laboratory of Explosion Science and Technology Beijing Institute of TechnologyBeijing 100081China

The increasing threat of explosions on the battle field and the terrorist action requires the development of more effective blast resistance materials and *** structure can support the external loads effectively by virtue of their spatial *** review of the excellent energy absorption property of auxetic structure,employing auxetic structure as core material in curved sandwich shows the potential to improve the protection *** this study,a novel cylindrical sandwich panel with double arrow auxetic(DAA) core was designed and the numerical model was built by *** to the complexity of the structure,systematic parameter study and optimal design are *** cases of optimal design were considered,case1 focuses on reducing the deflection and mass of the structure,while case2 focuses on reducing the deflection and increasing the energy absorption per unit *** study and optimal design were conducted based on Latin Hypercube Sampling(LHD)method,artificial neural networks(ANN) metamodel and the nondominated sorting genetic algorithm(NSGA-Ⅱ).The Pareto front was obtained and the cylindrical DAA structure performed much better than its equal solid panel in both blast resistance and energy absorption *** results can be used as a reference for different applications.

关键词： Auxetic structure Blast response Finite element analysis(FEA) Optimal design

Properties and photodetector applications of two-dimensional black arsenic phosphorus and black phosphorus

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Science China(Information Sciences) 2021年第4期64卷 120-133页

作者： Ruyue HAN shun feng Dong-Ming SUN Hui-Ming CHENG Shenyang National Laboratory for Materials Science Institute of Metal Research Chinese Academy of Sciences School of Materials Science and Engineering University of Science and Technology of China School of Physical Science and Technology ShanghaiTech University Shenzhen Geim Graphene Center Shenzhen International Graduate School Tsinghua University

Two-dimensional(2D) black arsenic phosphorus(b-AsP), as an alloy of black phosphorus(bP) with arsenic, has attracted great attention because of its outstanding electronic and optical properties,including high carrier mobility, tunable bandgap and in-plane anisotropy. B-AsP has a smaller bandgap(0.15–0.3 eV) than the b-P bandgap(0.3–2.0 eV), and thus can be used for mid-infrared *** addition, both of them can form various van der Waals(vdW) heterojunctions with other 2D materials to realize novel functional optoelectronic devices. Here, we compare the basic characteristics of b-AsP and b-P, including crystal structure, optical properties, band structure, electrical properties and stability, and we summarize the update progress of b-AsP in photo detection, including representatives of phototransistor and photodiode devices. In the last part, the future research directions are discussed.

关键词： black arsenic phosphorus crystal structure optical property electrical property photodetector

Experimental study on ceramic balls impact composite armor

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Defence Technology（防务技术） 2020年第2期16卷 408-416页

作者： Wei-zhan Wang Zhi-gang Chen shun-shan feng Tai-yong Zhao Underground Target Damage Technology National Defense Key Discipline Laboratory North University of ChinaTaiyuan 030051ShanxiChina State Key Laboratory of Explosion Science and Technology Beijing Institute of Technology100081BeijingChina

Ceramic balls represent a new type of damaging element,and studies on their damaging power of composite armor are required for a comprehensive evaluation of the effectiveness of various types of *** goal of this study was to determine the impact ofφ7 mm toughened Al2 O3 ceramic balls on a composite ceramic/metal *** influences of the ceramic panel and the thickness of the metal backing material on the destroying power of the ceramic balls were first *** on the agreement between numerical simulation,experimental results,and calculation models of the target plate resistance,the response mechanism of the ceramic balls was further *** results indicate that for a back plate of Q235 steel,with an increasing thickness of the ceramic panel,the piercing speed limit of the ceramic balls gradually increases and the diameter of the out-going hole on the metal back *** conditions were tested to assess the effects on the piercing speed,the diameter of the out-going hole,the micro-element stress,and the integrity of the recovered ceramic bowl.

关键词： Impact Penetration Infinitesimal Interface collapse

Blast response of continuous-density graded cellular material based on the 3D Voronoi model

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Defence Technology（防务技术） 2018年第5期14卷 433-440页

作者： Xu-ke Lan shun-shan feng Qi Huang Tong Zhou State Key Laboratory of Explosion Science and Technology Beijing Institute of Technology

One-dimensional blast response of continuous-density graded cellular rods was investigated theoretically and numerically. Analytical model based on the rigid-plastic hardening(R-PH) model was used to predict the blast response of density-graded cellular rods. Finite element(FE) analysis was performed using a new model based on the 3 D Voronoi technique. The FE results have a good agreement with the analytical predictions. The blast response and energy absorption of cellular rods with the same mass but different density distributions were examined under different blast loading. As a blast resistance structure, cellular materials with high energy absorption and low impulse transmit is attractive. However, high energy absorption and low impulse transmit cannot be achieved at the same time by changing the density distribution. The energy absorption capacity increases with the initial blast pressure and characteristic time of the exponentially decaying blast loading. By contract, when the blast loading exceeds the resistance capacity of cellular material, the transmitted stress will be enhanced which is detrimental to the structure being protected.

关键词： Gradient Blast response Cellular material 3D Voronoi model

Phagocytic Uptake of Nosema bombycis (Microsporidia) Spores by Insect Cell Lines

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Journal of Integrative Agriculture 2012年第8期11卷 1321-1326页

作者： CAI shun-feng LU Xing-meng QIU Hai-hong LI Ming-qian feng Zhen-zhen Laboratory of Invertebrate Pathology Zhejiang UniversityHangzhou 310058P.R.China

Microsporidia are highly specialized obligate intracellular parasites that can infect a wide variety of animals ranging from protists to mammals. The classical concept of the parasite invasion into a host cell involves its polar tube acting as a needle-syringe system. However, recent studies show microsporidian spores can also gain access to host cells by phagocytosis. The present study investigated the phagocytic uptake process of causative agent of the pebrine disease, Nosema bombycis, in several insect cell lines. We observed KOH-treated spores and cold-storaged spores can be easily uptaken by all the studied cell types 4 h post inoculation. In contrast, large numbers of freshly recovered spores remained in the culture medium. To further investigate the intracellular fates of KOH-treated spores and cold-storaged spores, electron and fluorescence microscopy were performed. No intracellular germination or subsequent parasite development were observed. Intracellular spores can be detected in host cells by polyclonal antibody 7 d post inoculation, suggesting phagocytized N. bombycis could not be digested by these non-professional phagocytes. Our results suggest that, phagocytic uptake of N. bombycis spores might represent a defense mechanism of the host cells and the intact spore wall barrier enable freshly recovered spores to keep resistance to this mechanism.

关键词： Microsporidia Nosema bombycis phagocytosis cell invasion

Ribavirin and IFN-α combination therapy induces CD4+ T-cell proliferation and Th1 cytokine secretion in patients with chronic hepatitis B

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World Journal of Gastroenterology 2007年第41期13卷 5440-5445页

作者： Fen-Yu Ren Hai Jin Xi-Xu Piao feng-shun Piao Department of Gastroenterology and Hepatology Yanbian University Hospital Yanji 133000 Jilin Province China

AIM: To investigate the anti-viral mechanism of combination therapy of interferon (IFN)-α and ribavirin in patients with chronic hepatitis B. METHODS: Twenty patients were assigned to receive either IFN-α plus ribavirin (group A,n = 14) or no treatment as a control (group B,n = 6). Patients were analyzed for T-cell proliferative responses specific for hepatitis B virus (HBV)-antigen and cytokine production by peripheral blood mononuclear cells (PBMCs). RESULTS: Combination therapy induced HBV-antigen specific CD4+ T-cell proliferative responses in four patients (28.6%). Production of high levels of HBV-specific IFN-γ,tumor necrosis factor (TNF)-α,interleukin (IL)-12 by PBMCs was found in five patients (35.7%),who showed significantly lower HBV DNA levels in serum at 12 mo after treatment ended (P = 0.038) and at 24 mo of follow-up (P = 0.004) than those without high levels of cytokine production. CONCLUSION: HBV-antigen specific CD4+ T cells may directly control HBV replication and secretion of anti-viral T helper 1 (Th1) cytokines by PBMCs during combination therapy of chronic hepatitis B with ribavirin and IFN-α.

关键词： Hepatitis B Interferon-alpha Ribavirin CD4+ T cells Th1

Isatin hybrids and their anti-tuberculosis activity

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Chinese Chemical Letters 2017年第2期28卷 159-167页

作者： Zhi Xu Shu Zhang Chuan Gao Jing Fan feng Zhao Zao-Sheng Lv Lian-shun feng Wuhan University of Science and Technology 430081 China Pony Testing International Group Wuhan 430034 China WuXi AppTec Wuhan 430075 China Hengshui University Hengshui 053000 China Synthetic and Functional Biomolecules Center Peking University Beijing 100871 China

Tuberculosis（TB） is one of the most common and even fatal infectious diseases known to *** of new cases are reported every year over the world,and one-third of the world＇s population is potentially infected with mycobacteria tuberculosis（MTB）.Research to develop novel anti-TB drugs led to the identification of several isatin-based antimycobacterial agents,among which a number of potential candidates displayed excellent antimycobacterial activity and were found to be free of *** review outlines the advances in the application of isatin hybrids as antimycobacterial agents and the critical aspects of design and structure-activity relationship of these derivatives.

关键词： Isatin hybrids Mycobacterium tuberculosis Quinolone Structure-activity relationship Review

RNA m6A dynamic modification mediated by nucleuslocalized FTO is involved in follicular reserve

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Zoological Research 2024年第2期45卷 415-428页

作者： Jiao-Na Zhang Rui-Ting Wang Francesca-Gioia Klinger shun-feng Cheng Wei Shen Xiao-feng Sun College of Life Sciences Key Laboratory of Animal Reproduction and Germplasm Enhancement in Universities of ShandongQingdao Agricultural UniversityQingdaoShandong 266109China Department of Biomedicine and Prevention University of Rome Tor VergataRome 00133Italy

In eukaryotic organisms,the most common internal modification of messenger RNA(m RNA)is N6-methyladenosine(m6A).This modification can be dynamically and reversibly controlled by specific enzymes known as m6A writers and *** fat-mass and obesity-associated protein(FTO)catalyzes RNA demethylation and plays a critical role in various physiological and pathological *** research identified dynamic alterations in both m6A and FTO during the assembly of primordial follicles,with an inverse relationship observed for m6A levels and nuclear-localized FTO *** of Fto small interfering RNA(si RNA)altered the expression of genes related to cell proliferation,hormone regulation,and cell chemotaxis,and affected RNA alternative *** of the full-length Fto gene led to changes in m6A levels,alternative splicing of Cdk5,cell proliferation,cell cycle progression,and proportion of primordial ***,overexpression of Fto lacking a nuclear localization signal(NLS)did not significantly alter m6A levels or primordial follicle *** findings suggest that FTO,localized in the nucleus but not in the cytoplasm,regulates RNA m6A demethylation and plays a role in cell proliferation,cell cycle progression,and primordial follicle *** results highlight the potential of m6A and its eraser FTO as possible biomarkers and therapeutic targets.

关键词： m6A FTO Ovary Primordial follicle assembly Alternative splicing