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Acta Pharmaceutica Sinica B 2022年第3期12卷 1254-1270页

作者： shuhua shan Jinping Niu Ruopeng Yin Jiangying Shi Lizhen Zhang Caihong Wu Hanqing Li Zhuoyu Li Institute of Biotechnology Key Laboratory of Chemical Biology and Molecular Engineering of National Ministry of EducationShanxi UniversityTaiyuan 030006China School of Life Science Shanxi UniversityTaiyuan 030006China

Molecular targeted therapy has become an emerging promising strategy in cancer treatment,and screening the agents targeting at cancer cell specific targets is very desirable for cancer *** previous study firstly found that a secretory peroxidase of class III derived from foxtail millet bran(FMBP)exhibited excellent targeting anti-colorectal cancer(CRC)activity in vivo and in vitro,whereas its underlying target remains *** highlight of present study focuses on the finding that cell surface glucose-regulated protein 78(cs GRP78)abnormally located on CRC is positively correlated with the anti-CRC effects of FMBP,indicating it serves as a potential target of FMBP against ***,we demonstrated that the combination of FMBP with the nucleotide binding domain(NBD)of cs GRP78interfered with the downstream activation of signal transducer and activator of transcription 3(STAT3)in CRC cells,thus promoting the intracellular accumulation of reactive oxygen species(ROS)and cell grown *** phenomena were further confirmed in nude mice tumor ***,our study highlights cs GRP78 acts as an underlying target of FMBP against CRC,uncovering the clinical potential of FMBP as a targeted agent for CRC in the future.

关键词： Foxtail millet bran FMBP csGRP78 STAT3 ROS Colorectal cancer

Gut microbiota remodeling drived by dietary millet protein prevents the metabolic syndrome

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Food Science and Human Wellness 2024年第4期13卷 1987-2001页

作者： shuhua shan Ruopeng Yin Jiangying Shi Lizhen Zhang Jiaqi Zhou Qinqin Qiao Xiushan Dong Wenjing Zhao Zhuoyu Li Institute of Biotechnology Key Laboratory of Chemical Biology and Molecular Engineering of National Ministry of EducationShanxi UniversityTaiyuan 030006China School of Life Science Shanxi UniversityTaiyuan 030006China shanxi Bethune Hospital Shanxi Academy of Medical SciencesTongji Shanxi HospitalThird Hospital of Shanxi Medical UniversityTaiyuan 030032China Department of Biology Biological Science and Technology CollegeTaiyuan Normal UniversityJinzhong 030619China

Metabolic syndrome(Met S)is a chronic disease associated with the disturbance of gut microbiota *** derived from gut microbes play essential roles in Met S prevention and ***,we focused on the inhibitory effect of the extract of millet bran protein(EMBP)on a high-fat diet(HFD)-induced Met S,aiming to identify gut microbiota and their metabolites that involve in the anti-Met S activity of *** obesity,chronic inflammation,insulin resistance in Met S mouse models were abolished after EMBP *** protective mechanism of EMBP against HFD-induced Met S may depend on improved gut barrier *** microbiome analysis,we found that EMBP supplementation improved gut microbiome dysbiosis in Met S mice,specifically upregulating Bacteroides *** fecal microbiota transplantation(FMT)also demonstrated this *** addition,metabolomic analysis showed that EMBP mediates metabolic profiling reprogramming in Met S ***,a microbiota-derived metabolite,gamma-aminobutyric acid(GABA),is enriched by *** addition,exogenous GABA treatment produced a similar protective effect to EMBP by improving NRF2-dependent gut barrier function to protect HFDinduced Met *** results suggest that EMBP suppress host Met S by remodeling of gut microbiota as an effective candidate for next-generation medicine food dual purpose dietary supplement to intervene in MetS.

关键词： Metabolic syndrome Gut microbiota Extract of millet bran protein Gamma-aminobutyric acid Gut barrier function

Polyphenol from foxtail millet bran alleviates experimental colitis in mice by remodulating intestinal fungal community

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Food Science and Human Wellness 2024年第6期13卷 3339-3350页

作者： Shuiling He Ruipeng Yang Jiangying Shi Ning An shuhua shan Zhuoyu Li Xiushan Dong Institute of Biotechnology Key Laboratory of Chemical Biology and Molecular Engineering of National Ministry of EducationShanxi UniversityTaiyuan 030006China General Surgery Department Shanxi Bethune HospitalShanxi Academy of Medical SciencesTongji Shanxi HospitalThird Hospitalof Shanxi Medical UniversityTaiyuan 030032China

Inflammatory bowel disease(IBD)is a chronic relapsing-remitting systemic disease of the gastrointestinal tract,characterized by an inflammatory *** mycobiota community dysbiosis has been reported that is closely related to the development of *** previous findings indicated that polyphenol of the inner shell(BPIS)from foxtail millet bran could restore the gut microbiome and inhibit the progress of colorectal cancer(CRC).In the present study,we studied the anti-inflammatory potential of BPIS in the dextran sodium sulfate(DSS)-induced mouse colitis *** suggested that BPIS alleviated experimental colitis by restoring body weight,colonic length and protecting the epithelial architecture from damage by ***,we found that BPIS strengthened the gut barrier function and inhibited the activation of Wnt1/β-catenin *** sequence analysis indicated that BPIS remodeled the overall structure of the gut mycobiota from colitis mice toward that of the normal counterparts,including 1 phylum and 9 ***,BPIS significantly increased the abundance of Aspergillus *** further verified that BPIS significantly promoted the growth of *** in ***,BPIS has great potential to develop into an effective against IBD drug.

关键词： Foxtail millet bran Inflammatory bowel disease Gut barrier Wnt1/β-catenin Fungal community Aspergillus ruber

Bound polyphenol from foxtail millet bran suppresses the LPS-induced proinflammatory cytokines by ROS/mi R-149 signal pathway

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Bound polyphenol from foxtail millet bran suppresses the LPS...

中国生物化学与分子生物学会2016年全国学术会议

作者： Jiangying Shi shuhua shan Zhuoyu Li Institute of Biotechnology Key Laboratory of Chemical Biology and Molecular Engineering of National Ministry of Education Shanxi University

The maladjustment of pro-inflammatory and anti-inflammatory had been acknowledged as one of the chief causations of inflammatory diseases and even *** studies showed that plant-derived bound polyphenols exhibits significantly anti-inflammatory functions,and this activity plays a beneficial role in various health *** present study found

关键词： Foxtail millet bran BPIS ROS mi R-149 Anti-inflammation

来源： cnki会议评论

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藜麦活性蛋白的制备及抗结肠癌效应评价

中国食品学报 2022年第11期22卷 57-64页

作者：加英张雪琛史江颖单树花李卓玉山西大学生命科学学院太原030006 山西大学生物技术研究所化学生物学与分子工程教育部重点实验室太原030006

本研究以藜麦全谷为原料,采用丙酮-硫酸铵沉淀法经Q柱进一步分离纯化,结合MTT法确定了具有抗结肠癌活性的藜麦蛋白(WQP),并在细胞水平上探究WQP的抗结肠癌活性。采用MTT试验表明WQP能够显著抑制结肠癌DLD1和HCT-8细胞的增殖,并具有质量... 详细信息

本研究以藜麦全谷为原料,采用丙酮-硫酸铵沉淀法经Q柱进一步分离纯化,结合MTT法确定了具有抗结肠癌活性的藜麦蛋白(WQP),并在细胞水平上探究WQP的抗结肠癌活性。采用MTT试验表明WQP能够显著抑制结肠癌DLD1和HCT-8细胞的增殖,并具有质量浓度梯度依赖性,而对人正常结肠上皮细胞FHC无影响;细胞克隆形成试验及细胞形态学试验进一步证明了WQP具有显著的抗结肠癌作用;流式细胞技术检测结果表明WQP能够诱导结肠癌细胞凋亡。此外,细胞划痕试验表明WQP通过抑制DLD1、HCT-8细胞的增殖,阻滞了结肠癌细胞迁移。本研究为将WQP进一步开发成为天然抗结肠癌特医功能食品及保健品提供了理论依据,同时为藜麦蛋白的深度利用奠定了基础。

关键词：藜麦活性蛋白抗结肠癌凋亡抗迁移

麦麸结合态多酚对肝癌HepG-2细胞增殖及凋亡效应研究

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中国粮油学报 2021年第9期36卷 22-29页

作者：牛晋平单树花赵惠玲李卓玉化学生物学与分子工程教育部重点实验室山西大学生物技术研究所太原030006 太原师范学院生物系太原030031

以小麦麸皮为原材料,采用丙酮-碱消化法,获得麦麸中的多酚物质,结合态多酚(WBBP)和自由态多酚(WBFP),研究WBBP对肝癌HepG-2细胞增殖和凋亡的影响。结果表明,麦麸结合态多酚的得率和含量均高于自由态多酚,WBBP处理对肝癌HepG-2细胞增殖... 详细信息

以小麦麸皮为原材料,采用丙酮-碱消化法,获得麦麸中的多酚物质,结合态多酚(WBBP)和自由态多酚(WBFP),研究WBBP对肝癌HepG-2细胞增殖和凋亡的影响。结果表明,麦麸结合态多酚的得率和含量均高于自由态多酚,WBBP处理对肝癌HepG-2细胞增殖的抑制效应具有浓度依赖性,而且不同浓度的WBBP通过诱导HepG-2肝癌细胞周期在S期阻滞,显著抑制了细胞增殖,凋亡以及线粒体膜电位的结果显示,随着WBBP处理浓度的增加,HepG-2肝癌细胞的凋亡、线粒体膜电位下降的细胞比例显著(P<0.01)增加,Caspase 3、Caspase 8、Caspase 9的酶活性也显著(P<0.01)增加,结果显示,WBBP通过Caspase依赖的线粒体凋亡路径诱导HepG-2肝癌细胞凋亡,显著抑制其增殖发挥抗肝癌效应。

关键词：麦麸结合态多酚 HepG-2肝癌细胞抑制增殖诱导凋亡

溴甲烷熏蒸对土壤反硝化作用及nosZ型反硝化微生物群落结构的影响

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微生物学报 2015年第1期55卷 73-79页

作者：燕平梅乔宏萍赵文婧陈燕飞单树花曹坳程太原师范学院生物系山西太原030012 中国农业科学院植物保护研究所北京100193

【目的】探究溴甲烷熏蒸对土壤反硝化作用的影响及机制。【方法】本研究采用nos Z-PCR-RFLP(Restriction fragment length polymorphism)方法、nos Z-MPN-PCR(Most-Probable-Number-PCR)计数法和土壤硝酸根的消灭率方法研究溴甲烷熏蒸对土壤nos Z型反硝化细菌群落结构、数量及反硝化活性的影响。【结果】实验结果说明溴甲烷熏蒸剂熏蒸土壤100 d土壤的反硝化作用与对照无显著差异(P>0.05)。溴甲烷熏蒸土壤和对照土样nos Z型反硝化细菌群落的Margalef指数,shannon-Wiener和Evenness指数无显著差异(P>0.05)。溴甲烷熏蒸土壤和对照土壤中存在Uncultured bacterium partial rhodopsendomonas、pseudomonas fluorescens、Herbacspirillum、Mesorhizobium和Bradyrhizobium,并且此6种微生物均是试验土样和对照土样的优势种群;对照土壤中检测到Azospirillum、Rhizobium melibei和Nitrosospira multiformis,在溴甲烷熏蒸土壤中未检测到;而溴甲烷熏蒸土壤中检测到Uncultured Azospirillum,Mesorhizobium在对照土壤中未检测到。通过nos Z-MPN-PCR计数法得出反硝化细菌数量比对照低1.4倍。【结论】说明溴甲烷熏蒸100d土壤的nos Z型反硝化微生物群落组成和反硝化细菌数量发生变化,而土壤的反硝化作用与对照无显著差异。

关键词：溴甲烷熏蒸反硝化细菌 nosZ基因群落结构

酸枣自由态和结合态多酚对人主动脉平滑肌细胞表型转化的影响

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食品科学 2019年第13期40卷 116-122页

作者：李汉卿刘丰铭单树花鲁洋张晓莉李卓玉山西大学生命科学学院山西太原030006 山西大学生物技术研究所化学生物学与分子工程教育部重点实验室山西太原030006

为研究酸枣肉、酸枣仁、酸枣叶中自由态和结合态多酚对人主动脉平滑肌细胞(human aortic smooth muscle cell,HASMC)表型的转化作用,将酸枣肉、酸枣仁、酸枣叶粉碎后,采用乙醇-酸碱消化法提取多酚;并用福林-酚比色法测定多酚质量浓度,... 详细信息

为研究酸枣肉、酸枣仁、酸枣叶中自由态和结合态多酚对人主动脉平滑肌细胞(human aortic smooth muscle cell,HASMC)表型的转化作用,将酸枣肉、酸枣仁、酸枣叶粉碎后,采用乙醇-酸碱消化法提取多酚;并用福林-酚比色法测定多酚质量浓度,对酸枣肉、酸枣仁、酸枣叶中自由态和结合态多酚质量浓度进行比较评价;倒置显微镜和荧光染色法观察酸枣多酚干预对HASMC表型转化的影响。结果表明:3 种自由态多酚中,酸枣叶自由态多酚(Ziziphus jujuba leaves free polyphenol-ethyl alcohol,ZSLFP-E)得率最高,结合态多酚中酸消化法提取的酸枣叶结合态多酚(Ziziphus jujuba leaves bound polyphenol-2-ethyl alcohol,ZSLBP-2-E)得率最高;ZSLFP-E和酸消化法提取的酸枣仁结合态多酚(Ziziphus jujuba seeds bound polyphenol-2-ethyl alcohol,ZSSBP-2-E)可以明显促进HASMC的形态由合成型(峰谷状)向收缩型(梭形)转变,且呈现时间和剂量依赖性。因此,ZSLFP-E和ZSSBP-2-E可以有效促进HASMC由合成型向收缩型转变,在动脉粥样硬化的预防和治疗中具有一定的应用前景。

关键词：酸枣多酚提取表型转化

基于PCR-DGGE方法分析榨菜中乳酸菌群落结构

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食品科学 2016年第13期37卷 136-139页

作者：燕平梅乔宏萍赵文婧单树花王琪柴政陈燕飞太原师范学院生物系山西太原030619 山西大学生命科学学院山西太原030031

为了深入了解榨菜中的乳酸菌多样性以及影响因素,以市场销售含有辣椒和不含辣椒两种袋装榨菜为研究对象,测定榨菜中的食盐浓度以及亚硝酸盐含量;通过变性梯度凝胶电泳（denaturing gradient gel electrophoresis,DGGE）法分离榨菜总微... 详细信息

为了深入了解榨菜中的乳酸菌多样性以及影响因素,以市场销售含有辣椒和不含辣椒两种袋装榨菜为研究对象,测定榨菜中的食盐浓度以及亚硝酸盐含量;通过变性梯度凝胶电泳（denaturing gradient gel electrophoresis,DGGE）法分离榨菜总微生物混合16S r DNA基因V7~V8片段,采用Quantity One软件分析乳酸菌物种丰富度、均匀度及物种多样性指数。结果表明：含辣椒的榨菜食盐浓度和亚硝酸盐含量均略低于不含有辣椒的榨菜;含有辣椒和不含辣椒的榨菜两者间物种多样性指数、丰富度和均匀度无显著差异（P〉0.05）。通过回收DGGE电泳带,经克隆后测定碱基序列、与Gen Bank库序列对比鉴定,不含有辣椒的榨菜5条回收聚合酶链式反应（polymerase chain reaction,PCR）-DGGE泳带a、b、c、d、e经鉴定分别与Pediococcus argentinicus CRL 776、Uncultured Lactobacillus *** DGGE gel band lx12、Uncultured bacterium clone 11.02-12、Uncultured bacterium clone 11.02-9、Uncultured Lactobacillus *** Px SC03相似度为98%、96%、97%、97%、97%。含有辣椒的榨菜4条回收PCR-DGGE泳带1、2、3、4经鉴定分别与Uncultured Lactobacillus sp.、Lactobacillus sakei A156、Lactobacillus sakei YY1、Lactobacillus sakei WJ1相似度为96%、97%、98%、97%。研究结果表明辣椒对榨菜中微生物群落结构无显著影响。

关键词：榨菜亚硝酸盐乳酸菌聚合酶链式反应-变性梯度凝胶电泳

谷子源可溶性膳食纤维对肝癌细胞HepG2的抑制作用

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山西大学学报(自然科学版) 2022年第4期45卷 1099-1106页

作者：李汉卿王瑾史江颖张晓莉单树花李卓玉山西大学生物技术研究所化学生物学与分子工程教育部重点实验室山西大学生命科学学院

以谷子、小米和谷糠为原料利用酶法结合化学法获得三种谷子源可溶性膳食纤维（SDF）。通过MTT法检测其对人肝癌HepG2细胞存活率的影响，筛选出最具抗肝癌活性的谷子源可溶性膳食纤维；采用克隆形成实验、细胞划痕实验、流式细胞仪评估... 详细信息

以谷子、小米和谷糠为原料利用酶法结合化学法获得三种谷子源可溶性膳食纤维（SDF）。通过MTT法检测其对人肝癌HepG2细胞存活率的影响，筛选出最具抗肝癌活性的谷子源可溶性膳食纤维；采用克隆形成实验、细胞划痕实验、流式细胞仪评估抗肝癌效果最显著的谷子源可溶性膳食纤维对HepG2单细胞克隆形成、迁移及凋亡的影响。结果表明：三种谷子源可溶性膳食纤维均能够抑制人肝癌HepG2细胞的增殖，揭示谷糠可溶性膳食纤维的抗肝癌效果尤为显著，并具有剂量依赖性（P0.05）。此外，克隆形成实验证明谷糠可溶性膳食纤维可显著减少HepG2细胞的克隆存活（P<0.05）；细胞划痕结果揭示谷糠源膳食纤维能够有效抑制HepG2细胞的迁移（P<0.05）；并且流式细胞术检测结果表明谷糠可溶性膳食纤维能够显著诱导HepG2细胞凋亡（P<0.05）。

关键词：谷糠制备可溶性膳食纤维抗肝癌