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Enhanced hydrolytic removal of tylosin in wastewater using polymer-based solid acid catalysts converted from polystyrene

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Journal of Environmental Sciences 2023年第4期126卷 287-296页

作者： Shiling Li shuhan sun Fei Qi Xiaomin Dou Beijing Key Lab for Source Control Technology of Water Pollution College of Environmental Science and EngineeringBeijing Forestry UniversityBeijing 100083China Engineering Research Center for Water Pollution Source Control and Eco-remediation College of Environmental Science and EngineeringBeijing Forestry UniversityBeijing 100083China

Antibiotic production wastewater usually contains high concentrations of antibiotic residues,which can cause instability and deterioration of biological wastewater treatment units and also domestication and proliferation of antibiotic-resistance *** effective pretreatment on antibiotics production wastewater is expected to selectively reduce the concentration of antibiotics and decrease the toxicity,rather than mitigate organic and other contaminants before further *** this work,two polymer-based solid acids,PS-S and CPS-S bearing high concentrations of-SOH_(3)groups (up to 4.57 mmol/g),were prepared and successfully used for hydrolytic mitigation of 100 mg/L tylosin within 20 *** co-existence of high concentrations of COD and humic substances did not affect the mitigation of tylosin obviously,while more than 500 mg/L of nitrogenous compounds suppressed the hydrolytic *** and reuse experiments showed that the solid acids performed well in five cycles after *** transformation products (P1,P2 and P3)were identified using UPLC-QTOF-MS/*** moieties including mycarse,mycaminose,and mycinose detached and released simultaneously or in order from the 16-member lactone ring through desugarization,which led to a dramatic decrease in antibacterial activity as revealed by cytotoxicity evaluations using *** estimation indicated the acute toxicities of the hydrolyzed products to model species (e.g.,fish,daphnid and green algae) were classified as“not harmful”.This work suggested an effective and selective method to pretreat tylosin-contained production wastewater by using polymer-based solid *** results will shed light on effective elimination of antibiotics pollution from pharmaceutical industries through strengthening the pretreatments.

关键词： Polymer Sulfonation Solid acid catalysts Tylosin hydrolysis Antibiotic

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综合医院耐碳青霉烯类肠杆菌的分布特点及耐药性分析

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中国现代医生 2024年第20期62卷 108-112页

作者：田昕吴悦孙姝晗王中新安徽医科大学第一附属医院检验科安徽合肥230022

目的通过分析耐碳青霉烯类肠杆菌科细菌(carbapenem-resistant enterobacteriaceae,CRE)感染的流行模式和耐药性,旨在为大型综合医院的感染防控和临床管理提供依据和建议。方法采用回顾性方法选取笔者医院2019年至2022年住院患者分离出... 详细信息

目的通过分析耐碳青霉烯类肠杆菌科细菌(carbapenem-resistant enterobacteriaceae,CRE)感染的流行模式和耐药性,旨在为大型综合医院的感染防控和临床管理提供依据和建议。方法采用回顾性方法选取笔者医院2019年至2022年住院患者分离出的609株CRE菌株,分析其标本来源、病原菌分布、科室分布及耐药性等。结果4年共检出肠杆菌6656株,CRE为609株,其中以肺炎克雷伯为主(354株,58.13%),其次为阴沟肠杆菌(82株,13.46%)和大肠埃希菌(77株,12.65%),检出CRE最多的科室为重症监护室(44.50%),其次为烧伤修复科(13.79%)、心脏大血管外科(8.87%)和肿瘤科(6.4%),标本来源依次为痰液(46.96%)、分泌物(15.60%)、尿液(13.30%)、血液(8.7%)。药敏结果显示,CRE菌株除对替加环素、多黏菌素和米诺环素等敏感(1.31%、0.56%和7.22%),对阿米卡星、磷霉素以及复方新诺明等耐药率较低(23.65%、35.14%、35.96%),对其余临床常用抗菌药物耐药率均>50%。结论2019至2022年CRE检出率总体上呈现上升趋势,对临床常用抗生素大多表现为多重耐药,应注意加强对抗菌药物的合理使用,加大对细菌耐药监管力度,遏制CRE的广泛传播。

关键词：耐碳青霉烯类肠杆菌科细菌药敏试验抗菌药物耐药性

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Influence and related mechanism of Retn gene expression on glucose uptake in 3T3-L1 cells

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Frontiers of Medicine 2007年第3期1卷 269-273页

作者： LI Yahui LI Huaixing DONG Shiyuan YU Chao JIANG Yu sun shuhan Institute for Nutritional Science Shanghai Institute for Biological SciencesChinese Academy of ScienceShanghai 200031China Center for Healthy Food Evaluation State Food and Drug AdministrationBeijing 100061China Department of Medical Genetics College of Basic Medical ScienceSecond Military Medical UniversityShanghai 200433China

The aim of this article was to investigate the influence and the related mechanism of the Retn gene on glucose uptake and insulin resistance in 3T3-L1 *** was used to determine glucose uptake in 3T3-L1 cells with different Retn gene expression levels,whether cells were stimulated by insulin or ***-PCR and real-time RT-PCR analysis were used to determine the mRNA levels of several glucose transport proteins in 3T3-L1 cells with different Retn gene expression levels,including insulin receptor substrate-1(IRS-1),phosphatidylinositol 3-kinase(PI-3K),AKT-2,glucose transporter-4(GLUT-4),p38 mitogen-activated protein kinase(p38MAPK)and glycogen synthase kinase-3b(GSK-3b).The glucose uptake decreased with the increase in Retn gene expression in 3T3-L1 cells,which was independent of whether the cells were stimulated by insulin or *** mRNA expression of two signal proteins PI-3K and AKT-2 decreased and the other two signal proteins,GSK-3b and p38MAPK,increased with Retn overexpression in 3T3-L1 *** could induce insulin resistance in adipocytes,which might be related to the changes of some proteins in PI-3K and Ras pathways.

关键词： Resistin Retn gene insulin glucose uptake 3T3-L1 cells

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p53 exerts anticancer effects by regulating enhancer formation and activity

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Journal of Biomedical Research 2024年第4期38卷 334-347页

作者： shuhan Chen Xuchun Wang Nan Yang Yuechi Song He Cheng Yujie sun Key Laboratory of Human Functional Genomics of Jiangsu Province School of Basic Medical SciencesNanjing Medical UniversityNanjingJiangsu 211166China Department of Cell Biology School of Basic Medical SciencesNanjing Medical UniversityNanjingJiangsu 211166China Jiangsu Key Lab of Cancer Biomarkers Prevention and TreatmentCollaborative Innovation Center for Personalized Cancer MedicineNanjing Medical UniversityNanjingJiangsu 211166China

The abnormality of the p53 tumor suppressor is crucial in lung cancer development,because p53 regulates target gene promoters to combat *** studies have shown extensive p53 binding to enhancer ***,whether p53 exerts a tumor suppressor role by shaping the enhancer landscape remains poorly *** the current study,we employed several functional genomics approaches to assess the enhancer activity at p53 binding sites throughout the genome based on our established TP53 knockout(KO)human bronchial epithelial cells(BEAS-2B).A total of 943 active regular enhancers and 370 super-enhancers(SEs)disappeared upon the deletion of p53,indicating that p53 modulates the activity of hundreds of enhancer *** found that one p53-dependent SE,located on chromosome 9 and designated as KLF4-SE,regulated the expression of the Krüppel-like factor 4(KLF4)***,the deletion of p53 significantly decreased the KLF4-SE enhancer activity and the KLF4 expression,but increased colony formation ability in the nitrosamines 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone-induced cell transformation ***,in TP53 KO cells,the overexpression of KLF4 partially reversed the increased clonogenic capacity caused by p53 ***,KLF4 expression also decreased in lung cancer tissues and cell *** appeared that overexpression of KLF4 significantly suppressed the proliferation and migration of lung cancer ***,our results suggest that the regulation of enhancer formation and activity by p53 is an integral component of the p53 tumor suppressor ***,our findings offer some novel insights into the regulation mechanism of p53 in lung oncogenesis and introduce a new strategy for screening therapeutic targets.

关键词： p53 enhancer tumor malignant transformation

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Hepatitis C Virus-induced upregulation of miR-155 promotes hepatocarcinogenesis by activating Wnt signaling

Hepatitis C Virus-induced upregulation of miR-155 promotes h...

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第十二次全国医学遗传学学术会议

作者： Yiliang Zhang Wei Wei Na Cheng shuhan sun Department of Medical Genetics Secondary military medicine university

Background & Aims:Hepatitis C virus(HCV) infection usually induces chronic hepatic inflammation,which favors the initiation and progression of hepatocellular carcinoma. Moreover,microRNA-155(miR-155) plays an important role in regulating both inflammation and ***,little is known about whether and how miR-155 provides the link between inflammation and *** & Results:In this study,we found that miR-155 levels were markedly increased in patients infected with ***-155 transcription was regulated by NF-κB,and p300 increased NF-κB-dependent miR-155 *** overexpression of miR-155 significantly inhibited hepatocyte apoptosis and promoted cell proliferation,whereas miR-155 inhibition induced G0/G1 *** miR-155 resulted in nuclear accumulation ofβ-catenin and a concomitant increase in cyclin D1,c-myc and survivin. Gain-of-function and loss-of-function studies demonstrated that miR-155 promoted hepatocyte proliferation and tumorigenesis by increasing Wnt signaling in vitro and in vivo,and DKK1(Wnt pathway inhibitor) overexpression inhibited the biological role of miR-155 in ***, adenomatous polyposis coli(APC),which negatively regulates Wnt signaling,was identified as the direct and functional target of ***:HCV-induced miR-155 expression promotes hepatocyte proliferation and tumorigenesis by activating Wnt *** present study provides a better understanding of the relationship between inflammation and tumorigenesis, and thus may be helpful in the development of effective diagnosis and treatment strategies against HCV-HCC.

关键词： HCV HCC miR-155 Wnt Apc.

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Early Warning System of Risk in Dairy Cows with Inactive Ovaries

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Journal of Biosciences and Medicines 2018年第11期6卷 74-88页

作者： Chang Zhao Shi Shu Jiang Zhang Yunlong Bai shuhan sun Yuxi Song Cheng Xia College of Animal Science and Veterinary Medicine Heilongjiang Bayi Agricultural University Daqing China

The incidence of Inactive ovaries of dairy cows in China is relatively high. There is no complete early warning system for the occurrence of ovarian quiescence in clinical cows. This test provides early warning indicators for clinical prediction of ovary cessation in dairy cows. This experiment selected blood samples of dairy cows from 60 to 90 days postpartum in the inactive ovaries group and control group. Differential proteins were selected on the basis of proteomics, three energy indexes: AST, Glu, NEFA. Four reproductive hormones: E2, P4, FSH, LH, and four differentially expressed proteins: IGFBP-2, AHSG, APO-A4, and RBP-4. Key enzyme activities: ALDOB, LDHB, ITIH3, GPX3, SPAM1, PKM2. The ELISA test kit was used to detect the content and activity of the above markers in the test bovine serum. Through correlation analysis, binary logistic regression modeling and ROC analysis, a single indicator early warning technique for APOA4 and ITIH3 was established. The early warning values were APOA4 > 28.825 μg/L and ITIH3 > 195.07 ng/L. A multi-index early warning system based on potential biomarkers of APOA4 + ITIH3 and APOA4 + ITIH3 + E2 was established. The former had an early warning value of: APOA4 > 19.55 μg/I;ITIH3 > 191.14 ng/L;the latter has an early warning value: APOA4 > 47.56 μg/L, ITIH4 > 187.80 ng/L, E2 < 69.63 ng/L.

关键词： Inactive Ovaries of Dairy Cows APOA4 ITIH3 E2 ROC

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THE INFLUENCE OF REPLENISHING KIDNEY HERBS ON CALBINDIND9k GENE AND ITS EXPRESSION IN RATS MODEL WITH OSTEOPOROSIS

THE INFLUENCE OF REPLENISHING KIDNEY HERBS ON CALBINDIND9k G...

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第二届国际骨质疏松研讨会

作者： Liu HeDi Li En sun shuhan Wang Zuen Xia Department of Biochemistry HeBei Medical College

＜正＞INTRODUCTION Primary osteoporosis is a metablic disease of general bone loss because of endocrine disorder with reducing bone strenth,bone fuzzy and mechanical incompetence（fracture during normal mechanical usage）,the mechanism of the osteporosis remains unclear in *** is hypothesis that it related to the imbalance of many hormones influence on calcium and phosphate *** is all know that the CaBP-D9K is found to concentrate in the proximal intestine,play a role in intestinal calcium

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DNA Methylomes in CpG Island Methylator Phenotype(CIMP) Colon Cancer:new screening marks proposal

DNA Methylomes in CpG Island Methylator Phenotype(CIMP) Colo...

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第十二次全国医学遗传学学术会议

作者： Hongli Yan Guan’nan Tang Yiliang Zhang Yuzhao Wang shuhan sun Department of medical genetics Second Military Medical University

Background & Aims:DNA methylation is essential for normal development and is frequently altered in *** Island Methylator Phenotype(CIMP) is a subset of colorectal cancers was postulated to have a higher propensity for DNA ***,the validity of CIMP,its molecular basis,and its prognostic values are highly *** aims of this study were to(1) compare and characterize the unique features of DNA methylomes of CIMP with Non-CIMP colonrectal cancers;(2) propose a new panel of valuable markers for CIMP screening. Methods & Results:Here we utilized MBD-isolated Genome Sequencing(MiGS) to map the DNA methylomes of three sets of normal colon,non-CIMP colon cancers,and CIMP colon *** regions only robust hypermethylated in CIMP tumors were evaluated in 120 colorectal tumors by Bisulfite genomic sequencing or Methylation Specific PCR(MSP).We found 4,800 sites were hypermethylated in both non-CIMP and CIMP colon cancers when compared with normal *** those sites,318 sites were robust hypermethylated in CIMP tumors only. Methylation of these CpG islands regulates 122 key genes in tumorgenesis and progress.A panel of 5 new markers was proposed for CIMP screening in 120 consecutive colon samples,13.3% (16/120) were methylated in all 5 markers and 87.5%(14/16) were *** the markers described by Weisenberger et al.,22.5%(27/120) were methylated in all 5 markers and only 51.8%(14/27) were MSI ***:CIMP are a subset of colorectal cancer has unique features in DNA *** genes only hypermethylated in CIMP tumors shed lights on exploration the mechanism of CIMP tumorgenesis and provide new targets for cancer therapies. The new panel of markers facilitates CIMP screening in sporadic colorectal cancers.

关键词： Colorectal cancer CpG Island Methylator Phenotype MBD-isolated Genome Sequencing DNA methylome

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Silencing of Grainyhead-like-2(GRHL2) at the tumor edge by DNA methylation facilitates Epithelial-Mesenchymal Transition through targeting Rab25 and E-cadherin

Silencing of Grainyhead-like-2(GRHL2) at the tumor edge by D...

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第十二次全国医学遗传学学术会议

作者： Guan’nan Tang Hongli Yan Yiliang Zhang YuZhao Wang shuhan sun Department of medical genetics Second Military Medical University

Background & Aims:Epithelial-Mesenchymal Transition(EMT) plays a pivotal role in tumor invasion and *** it is well documented that EMT occurred predominantly at the tumor edge where it is induced by cytokines,the extracellular matrix environment,or hypoxia,the mechanism(s) underlying the tumor microenvironment-induced Epithelial-Mesenchymal Transition remains ***-like-2(GRHL2) is a new transcription factor involved in embryo development and epithelial *** of GRHL2 has been noted in several tumor types,including breast cancer,colon *** addition,it has been reported that GRHL2 regulated the epithelial apical junctional complex by targeting the tight junction gene claudin 4(Cldn4).These findings imply that GRHL2 might play a key role in Epithelial-Mesenchymal *** & Results:We systemically analyzed the expression of GRHL2 in colon and liver cancer *** control by querying oncomine database and real time PCR analysis,the resulted showed that GRHL2 was respectively down-regulated in 40%and 76%of colon cancer and liver *** investing the expression of GRHL2 in colon cancer by immunohistochemistry analysis indicated that GRHL2 was loss of expression at the tumor edge and surround fibroblast *** further found that promoter of GRHL2 was embedded in a large CpG island and methylated in tumor cells with a low level of *** by 5-aza-dc treatment significantly enhanced GRHL2 expression in SW480 and SW620 cells,revealed that GRHL2 is epigenetically silenced by DNA methylation. Overexprssion of GRHL2 in SW480 and SW620 promotes Epithelial-Mesenchymal Transition (MET).Real time PCR,Western blot and Immunoprecipitation(ChIP) assay indicated that over-expression of GRHL2 enhances E-cadherin and Rab25 expression by binding to the promoter of these two *** addition,we also found that overexpression of GRHL2 suppresses CD24 ***:These data indicated that

关键词： Colorectal cancer Liver cancer Grainyhead-like-2(GRHL2) DNA methylation Epithelial-Mesenchymal Transition(EMT)

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Long noncoding RNA H19 inhibits the growth and invasion of trophoblasts by inactivating Wnt/β-catenin signaling via downregulation of DDX3X

Long noncoding RNA H19 inhibits the growth and invasion of t...

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第9届中国中西医结合学会妇产科专业委员会第二次学术会议

作者： Yan Ma Ningxia sun Fei Sheng Yixuan Ji Haixia Ding Qing Zhang shuhan sun Wen Li

In our study, we highlights the importance of lnc RNA-H19 in the restriction of early placenta overgrowth and the transfer of genetic and epigenetic information from the entire genome. We examined the effects of H19 on the proliferation and invasion of human trophoblasts. To further explore the mechanism, RNA pulldown and mass spectrometry were used to identify lnc RNA-H19-binding proteins in human trophoblasts. In addition, changes in methylation of the H19 promoter region were determined using pyrophosphate sequencing *** results indicate that lnc RNA-H19 was upregulated in the spontaneous abortion samples, consistent with the hypomethylation of the differential methylation region(DMR) of lnc RNA-H19. Overexpression of lnc RNA-H19 inhibited trophoblast growth and invasion. Furthermore, our studies demonstrated that H19 inhibited Wnt-β-catenin pathway activation in trophoblasts by affecting DDX3 X function, thus clarifying the mechanism of action of H19.

关键词： Long noncoding RNA H19 early unexplained miscarriage trophoblast DDX3X Wnt-β-catenin signalling

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