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A bHLH transcription factor,CsSPT,regulates high-temperature resistance in cucumber

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Horticultural Plant Journal 2024年第2期10卷 503-514页

作者： Yonggui liang chenyu Yang Fangyan Ming Bingwei Yu Zhihua cheng Yixi Wang Zhengkun Qiu Xiaolan Zhang Bihao Cao shuangshuang Yan Key Laboratory of Biology and Genetic Improvement of Horticultural Crops(South china) Ministry of Agriculture and Rural AffairsGuangdong Vegetable Engineering and Technology Research CenterCollege of HorticultureSouth China Agricultural UniversityGuangzhouGuangdong 510642China State Key Laboratories of Agrobiotechnology Beijing Key Laboratory of Growth and Developmental Regulation for Protected Vegetable CropsMOE Joint Laboratory for International Cooperation in Crop Molecular BreedingChina Agricultural UniversityBeijing 100193China

High-temperature stress threatens the growth and yield of crops. Basic helix-loop-helix(bHLH) transcription factors(TFs) have been shown to play important roles in regulating high-temperature resistance in plants. However, the bHLH TFs responsible for high-temperature tolerance in cucumbers have not been identified. We used transcriptome profiling to screen the high temperature-responsive candidate bHLH TFs in cucumber. Here, we found that the expression of 75 CsbHLH genes was altered under high-temperature stress. The expression of the CsSPT gene was induced by high temperatures in TT(Thermotolerant) cucumber plants. However, the Csspt mutant plants obtained by the CRISPR-Cas9 system showed severe thermosensitive symptoms, including wilted leaves with brown margins and reduced root density and cell *** Csspt mutant plants also exhibited elevated H_(2)O_(2) levels and down-regulated photosystem-related genes under normal ***, there were high relative electrolytic leakage(REC), malondialdehyde(MDA), glutathione(GSH), and superoxide radical(O_(2)^(·-)) levels in the Csspt mutant plants, with decreased Proline content after the high-temperature treatment. Transcriptome analysis showed that the photosystem and chloroplast activities in Csspt mutant plants were extremely disrupted by the high-temperature stress compared with wildtype(WT) plants. Moreover, the plant hormone signal transduction, as well as MAPK and calcium signaling pathways were activated in Csspt mutant plants under high-temperature stress. The HSF and HSP family genes shared the same upregulated expression patterns in Csspt and WT plants under high-temperature conditions. However, most bHLH, NAC, and bZIP family genes were significantly down-regulated by heat in Csspt mutant plants. Thus, these results demonstrated that CsSPT regulated the high-temperature response by recruiting photosynthesis components, signaling pathway molecules, and transcription factors. Our results pro

关键词： Cucumber bHLH CsSPT Photosynthesis High temperature

Construction and initial analysis of five Fosmid libraries of mitochondrial genomes of cotton(Gossypium)

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Chinese Science Bulletin 2013年第36期58卷 4608-4615页

作者： li shuangshuang liU GuoZheng chen zhiwen WANG YuMei li PengBo HUA JinPing Department of Plant Genetics and Breeding College of Agronomy and Biotechnology/Key Laboratory of Crop Heterosis and Utilization of Ministry of Education/Beijing Key Laboratory of Crop Genetic Improvement China Agricultural University Institute of Cash Crops Hubei Academy of Agricultural Sciences Cotton Research Institute Shanxi Academy of Agricultural Sciences

Cotton(Gossypium)is an important crop providing textile fiber and edible *** gain the insights into mechanism of the cytoplasmic male sterility(CMS)inheritance,we constructed five fosmid libraries of mitochondrial genomes from mitotype of *** Brandegee.(one CMS line and its restorer),mitotype of *** L.(one CMS line and its maintainer),and *** *** numbers of the clones in these libraries ranged from 1152 to 2016 with an average insert size of 36.2 to 38.4 kb,equivalent to 70–119.3 *** libraries were screened with 28 markers derived from the conservative sequences and yielded 22,19,26,21,and 23 positive clones,*** positive clones were used to construct the physical map of *** *** line and *** *** that shared six syntenis regions.A total of 30 genes in nine clusters showed conservative and had high similarity with those in the mitochondrial genomes of cotton,Carica papaya,Cucurbita pepo and Nicotiana *** investigation indicated that gene rrn26 had two copies in all five cotton mitogenomes,while genes atp1,rrn5 and rrn18 had two copies only in *** *** positive clones and physical map are considered being useful resources in cotton genomics research.

关键词：线粒体基因组粘粒文库棉花细胞质雄性不育系阳性克隆 CMS系保守序列物理图谱

Daxx is reciprocally regulated by Mdm2 and Hausp

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Daxx is reciprocally regulated by Mdm2 and Hausp

第三届细胞结构与功能的信号基础研讨会

作者： Jun Tang china agricultural university,2 Yuanmingyuan W.Rd,haidian District,beijing 100193,china

Daxx is a multifunctional protein,regulating a wide range of important functions including apoptosis and ***,the way Daxx is regulated is poorly *** our previous studies,we have found that Daxx forms a complex with the E3 ubiquitin ligase Mdm2 and the deubiquitinase *** the present work,we show that Daxx is ubiquitinated by Mdm2 in both in vitro and in vivo systems and Mdm2 reduces Daxx expression upon *** further demonstrate that Hausp critically controls the cellular level of Daxx most likely by inducing Daxx *** results reveal Mdm2 and Hausp as important regulators for Daxx functions by controlling Daxx ubiquitination and stability.

关键词： Daxx Mdm2 Hausp Ubiquitination Deubiquitination

Sequencing and Analysis of the Mitochondrial Genome of Gossypium harknessii Cytoplasmic Male Sterile line

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Sequencing and Analysis of the Mitochondrial Genome of Gossy...

第十二届全国植物基因组学大会

作者： shuangshuang li,Binbin Lei,Guozheng liu,Jinping Hua~* College of Agricultrue and Biotechnology,china agricultural university,beijing 100193,china

Plant mitochondrial genomes are known for their *** evidences demonstrated that the organelle was important for plant sexual *** male sterility(CMS) was caused by incompatibility between the nucleus and mitochondria,and was closely associated with mitochondrial DNA (mtDNA) *** it will be helpful to understand the mechanism of CMS to obtain the full sequence of the genome. A Gossypium harknessii CMS line(2074A) was used in the ***-quality cotton mtDNA was ex-

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Transcriptional gene silencing independent on siRNA-directed DNA methylation in Arabidopsis:characterization of ros1 suppressors and identification of more ROS genes

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Transcriptional gene silencing independent on siRNA-directed...

第十一届全国植物基因组学大会

作者： Zhizhong Gong~* State Key Laboratory of Plant Physiology and Biochemistry,College of Biological Sciences,china agricultural university,No.2 Yuanmingyuan Xilu,haidian District,beijing,100193,P.R.china

ROS1 encodes a DNA demethylation protein which actively removes the RD29A promoter DNA methylation caused by siRNA-directed DNA methylation in a T-DNA locus,and also keeps the transcription of 35S-NPTII in the same T-...

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The Gene,tRNA and Intron Variation Revealed by Comparative and Phylogenetic Analysis of Plant Mitochondrial Genomes

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The Gene,tRNA and Intron Variation Revealed by Comparative a...

第十二届全国植物基因组学大会

作者： Binbin Lei,shuangshuang li,Guozheng liu,Jinping Hua~* Key Laboratory of Crop Genetic Improvement and Genome of Ministry of Agriculture,College of Agronomy and Biotechnology, china agricultural university,beijing 100193,china

Frequent mitochondrial DNA recombination and rearrangement,RNA editing and chimeric Open Reading Frame(ORF) etc are likely the main reasons of Cytoplasmic Male Sterility(CMS).Studying the plant mitochondrial genomes helped us analyze the evolution dynamics of species and genetic mechanism of CMS. This research chose 2074A for material,which is a Gossypium harknessii cytoplasm sterile *** the mitochondrial genome of 2074A,annotated functional genes and amplified full length of all these genes. 9 conserved genes which contained nad3,nad4L,nad6,nad9,cob,cox1,cox3,atp1 and atp6,were chosen

关键词： Gossypium harknessii Phylogenetic tree Mitochondrial DNA Cytoplasmic Male sterility

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肉鸡胫骨软骨症发病趋势及组织学研究

畜牧兽医学报 1994年第1期25卷 5-12页

作者：罗兰高齐瑜中国农业科学院畜牧研究所北京农业大学兽医学院

本试验采用４８０只１日龄ＡｒｂｒｏＡｃｒｅｓ商品代仔公鸡，研究胫骨软骨症（ＴｉｂｉａｌＤｙｓｃｈ－ｏｎｄｒｏｐｌａｓｉａ，ＴＤ）的发生特征，胫骨软骨症随肉鸡生长的动态发展趋势以及与日粮氯和镁水平之间的关系。试验为２×... 详细信息

本试验采用４８０只１日龄ＡｒｂｒｏＡｃｒｅｓ商品代仔公鸡，研究胫骨软骨症（ＴｉｂｉａｌＤｙｓｃｈ－ｏｎｄｒｏｐｌａｓｉａ，ＴＤ）的发生特征，胫骨软骨症随肉鸡生长的动态发展趋势以及与日粮氯和镁水平之间的关系。试验为２×２×４因子设计，日粮氯、镁分别采用２个水平，氯为０．１５％和０．３５％，镁为０．１５％和０．４０％。时间因素为第三个因子，１０天为一个阶段，共４个阶段。试验期为４０天。实验结果表明，肉鸡胫骨软骨症的发生与发展具有明显的时间性并与肉鸡的生长速度有着紧密的联系。生长较快的鸡，其ＴＤ的发病率多集中在３０日龄之前。日粮氯和镁水平与时间因素共同影响肉鸡的生长速度和ＴＤ发病率，日粮氯元素诱发ＴＤ的作用在肉鸡３０日龄之前呈逐渐增加趋势，３０日龄后作用减弱。病理组织学的研究结果显示，生长快的肉鸡ＴＤ发病率高的机制是因为胫骨软骨繁殖区非成熟的软骨细胞极度增生而形成软骨栓。软骨栓内没有血管生成，不能及时供给软骨细胞发育成熟钙化所需的营养，从而进一步加剧了软骨栓的形成，最终导致胫骨软骨症。日粮氯和镁元素以某种方式影响胫骨软骨繁殖区内血管的生成和发育，以及软骨细胞发育所需的营养供给。

关键词：胫骨软骨症鸡病肉用鸡组织学

Technique and Policy Issues on Irrigation Agriculture Development and Countermeasures in china

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Technique and Policy Issues on Irrigation Agriculture Develo...

国际农业工程大会

作者： Fahu li, Dr.College of Hydraulic and Civil Engineering, china agricultural university, POB 295, No. 17, Qinghua Donglu, haidian District, beijing 100083, china

＜正＞ Agriculture development is comprehensively affected by natural resource, society and economic development level. Agriculture is an industry with low economic profit but it is special important for society stability and country *** agriculture plays an important role on grain supply, but water resource shortage constrains its development. china is a developing country with vast population;Issues that

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乡村振兴视域下产业重构的理论解析与模式研究——以广西为例

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地理科学进展 2024年第3期43卷 434-445页

作者：徐雨利龙花楼屠爽爽姜棪峰广西大学公共管理学院南宁530004 中国科学院地理科学与资源研究所北京100101 南宁师范大学北部湾环境演变与资源利用教育部重点实验室南宁530001

快速城镇化进程中乡村地域人地关系日益复杂,乡村面临整合有限资源实现产业转型发展的现实难题。论文从乡村地域系统“要素—结构—功能”转变的视角出发,对乡村产业重构的概念内涵与驱动力进行阐释,并以广西为例,根据主导产业的发展方... 详细信息

快速城镇化进程中乡村地域人地关系日益复杂,乡村面临整合有限资源实现产业转型发展的现实难题。论文从乡村地域系统“要素—结构—功能”转变的视角出发,对乡村产业重构的概念内涵与驱动力进行阐释,并以广西为例,根据主导产业的发展方向对乡村产业重构的典型模式进行划分,基于此构建乡村产业重构的保障机制。结论如下:(1)乡村产业重构可理解为基于当地发展条件与外部市场环境,整合劳动力、土地、资本等基本生产要素,通过产业培育、调整、升级与转型,优化空间布局,延伸产业链条,完善产业体系,以实现乡村产业内部良性均衡发展与对外产生输出价值的过程,具有主体多元化与业态多样化的特征。(2)在产业重构的具体实践中,乡村地域系统的外源驱动力与内生发展力相互作用,共同推动产业发展相关的各要素耦合协调。(3)乡村产业重构包括农业种养专业化模式、传统文化产业化模式、乡村旅游驱动型模式、工业制造业集聚型模式与商贸物流服务型模式等5种典型模式。(4)政策方面应重点破除体制机制壁垒,引导关键生产要素向乡村地区集中;优化产业结构,完善产业布局,构建现代乡村产业体系;完善利益联结机制,坚持农民主体地位不动摇。通过综合施策,以期为乡村产业重构实践提供机制保障,助力乡村产业振兴。

关键词：乡村振兴乡村地域系统产业重构典型模式广西