Fosmid library screening of cotton restorer lines E5903 mitochondrial genome

作     者：Shuangshuang Li,Zhiwen Chen~* China Agricultural University,Beijing 100193,Haidian 

会议名称：《第十三届全国植物基因组学大会》

会议日期：2012年

学科分类：09[农学] 0901[农学-作物学] 

摘      要：In this paper,a Fosmid library of restorer lines E5903 mitochondrial genome was *** mitochondrial DNA was isolated by sucrose density gradient centrifugation *** DNA was randomly sheared and cloned into pCC1FOS vector,recombinant DNA was packaged with the Lambda Packaging Extracts,then transfected into *** strain *** best sheared parameter employed in the