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Anti-inflammatory mechanism of the non-volatile ingredients performance liquid chromatography-heated electron spray ionization-high resolution mass spectroscope and cell metabolomics

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Journal of Traditional Chinese Medicine 2024年第2期44卷 260-267页

作者： JING Wenguang LIN Xiaoyu LI Chu ZHAO Xiaoliang CHENG Xianlong WANG Penglong WEI Feng ma shuangcheng Institute of Control of Chinese Traditional Medicine and Ethnic Medicine National Institutes for Food and Drug ControlBeijing 102629China School of Traditional Chinese Medicine Beijing University of Chinese MedicineBeijing 102488China Beijing Key Laboratory of Traditional Chinese Medicine Basic Research on Prevention and Treatment of major Diseases Experimental Research CenterChina Academy of Chinese Medical SciencesBeijing 100700China

OBJECTIVE:To explore the anti-inflammatory components and mechanism of the non-volatile ingredients of patchouli.METHODS:High performance liquid chromatographyheated electron spray ionization-high resolution mass spectroscope(HPLC-HESI-HRMS)was used to analyze the chemical constituents of the non-volatile ingredients of patchouli.The anti-inflammatory activity of ingredients was evaluated using lipopolysaccharide(LPS)induced RAW264.7 cell inflammation model,and the antiinflammatory mechanism was investigated using multivariate statistical analysis of cell metabolomics.RESULTS:The non-volatile ingredients of patchouli were characterized by HPLC-HESI-HRMS,and 36 flavonoids and 18 other components were identified.These ingredients of patchouli not only had a good protective effect on the LPS-induced inflammation model of RAW264.7 cells,but also regulated the expression levels of arginine,L-leucine,cholesterol,fructose and sorbitol by down-regulating arginine metabolism,aminoacyl-tRNA biosynthesis,polyol/sorbitol pathway,so as to reduce inflammation and reduce cell damage.CONCLUSION:The non-volatile ingredients of patchouli had good anti-inflammatory effect and exerted its curative effect by regulating endogenous metabolic pathway to reduce inflammatory response.

关键词： Pogostemon non-volatile ingredients anti-inflammatory cell metabolomics

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Blockade of CD300A enhances the ability of human NK cells to lyse hematologic malignancies

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Cancer Biology & Medicine 2024年第4期21卷 331-346页

作者： shuangcheng Li Tianci Wang Xinghui Xiao Xiaodong Zheng Haoyu Sun Rui Sun Hongdi ma Zhigang Tian Xiaohu Zheng Hefei National Research Center for Physical Sciences at Microscale The CAS Key Laboratory of Innate Immunity and Chronic DiseaseSchool of Basic Medical SciencesCenter for Advanced Interdisciplinary Science and Biomedicine of IHMDivision of Life Sciences and MedicineUniversity of Science and Technology of ChinaHefei 230027China Institute of Immunology University of Science and Technology of ChinaHefei 230027China Hefei TG ImmunoPharma Corporation Limited Hefei 230601China Research Unit of NK Cell Study Chinese Academy of Medical SciencesBeijing 100864China Key Laboratory of Quantitative Synthetic Biology Shenzhen Institute of Synthetic BiologyShenzhen Institute of Advanced TechnologyChinese Academy of SciencesShenzhen 518055China

Objective: The human cluster of differentiation(CD)300A, a type-I transmembrane protein with immunoreceptor tyrosine-based inhibitory motifs, was investigated as a potential immune checkpoint for human natural killer(NK) cells targeting hematologic malignancies(HMs).Methods: We implemented a stimulation system involving the CD300A ligand, phosphatidylserine(PS), exposed to the outer surface of malignant cells. Additionally, we utilized CD300A overexpression, a CD300A blocking system, and a xenotransplantation model to evaluate the impact of CD300A on NK cell efficacy against HMs in in vitro and in vivo settings. Furthermore, we explored the association between CD300A and HM progression in patients.Results: Our findings indicated that PS hampers the function of NK cells. Increased CD300A expression inhibited HM lysis by NK cells. CD300A overexpression shortened the survival of HM-xenografted mice by impairing transplanted NK cells. Blocking PS–CD300A signals with antibodies significantly amplified the expression of lysis function-related proteins and effector cytokines in NK cells, thereby augmenting the ability to lyse HMs. Clinically, heightened CD300A expression correlated with shorter survival and an “exhausted” phenotype of intratumoral NK cells in patients with HMs or solid tumors.Conclusions: These results propose CD300A as a potential target for invigorating NK cell-based treatments against HMs.

关键词： NK cell CD300A phosphatidylserine immune checkpoint hematologic malignancy

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Antagonistic maDS-box transcription factors SEEDSTICK and SEPALLATA3 form a transcriptional regulatory network that regulates seed oil accumulation

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Journal of Integrative Plant Biology 2024年第1期66卷 121-142页

作者： shuangcheng He Yuanchang Min Zijin Liu Fang Zhi Rong ma Ankang Ge Shixiang Wang Yu Zhao Danshuai Peng Da Zhang Minshan Jin Bo Song Jianjun Wang Yuan Guo Mingxun Chen State Key Laboratory for Crop Stress Resistance and High‐Efficiency Production National Yangling Agricultural Biotechnology&Breeding CenterShaanxi Key Laboratory of Crop HeterosisCollege of AgronomyNorthwest A&F UniversityYangling 712100China State Key Laboratory for Crop Stress Resistance and High‐Efficiency Production Shaanxi Key Laboratory of AppleCollege of HorticultureNorthwest A&F UniversityYangling 712100China

Transcriptional regulation is essential for balancing multiple metabolic pathways that influence oil accumulation in seeds.Thus far,the transcriptional regulatory mechanisms that govern seed oil accumulation remain largely unknown.Here,we identified the transcriptional regulatory network composed of maDS-box transcription factors SEEDSTICK(STK)and SEPALLATA3(SEP3),which bridges several key genes to regulate oil accumulation in seeds.We found that STK,highly expressed in the developing embryo,positively regulates seed oil accumulation in Arabidopsis(Arabidopsis thaliana).Furthermore,we discovered that SEP3 physically interacts with STK in vivo and in vitro.Seed oil content is increased by the SEP3 mutation,while it is decreased by SEP3 overexpression.The chromatin immunoprecipitation,electrophoretic mobility shift assay,and transient dual-luciferase reporter assays showed that STK positively regulates seed oil accumulation by directly repressing the expression of MYB5,SEP3,and SEED FATTY ACID REDUCER 4(SFAR4).Moreover,genetic and molecular analyses demonstrated that STK and SEP3 antagonistically regulate seed oil production and that SEP3 weakens the binding ability of STK to MYB5,SEP3,and SFAR4.Additionally,we demonstrated that TRANSPARENT TESTA 8(TT8)and ACYL-ACYL CARRIER PROTEIN DESATURASE 3(AAD3)are direct targets of MYB5 during seed oil accumulation in Arabidopsis.Together,our findings provide the transcriptional regulatory network antagonistically orchestrated by STK and SEP3,which fine tunes oil accumulation in seeds.

关键词： seed oil accumulation SEP3 STK transcription factor transcriptional regulation

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Remote sensing and its applications using GNSS reflected signals:advances and prospects

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Satellite Navigation 2024年第1期5卷 10-51页

作者： Shuanggen Jin Adriano Camps Yan Jia Feng Wang manuel martin-Neira Feixiong Huang Qingyun Yan shuangcheng Zhang Zhongyu Li Komi Edokossi Dongkai Yang Zhiyu Xiao Zhongmin ma Weihua Bai Shanghai Astronomical Observatory Chinese Academy of SciencesShanghai200030China School of Surveying and Land Information Engineering Henan Polytechnic UniversityJiaozuo454003China Department of Signal Theory and Communications Technical University of CataloniaBarcelona08034Spain Department of Surveying and Geoinformatics Nanjing University of Posts and TelecommunicationsNanjing210023China School of Electronics and Information Engineering Beihang UniversityBeijing100191China European Space Research and Technology Centre(ESTEC) European Space Agency2200-AGNoordwijkThe Netherlands National Space Science Center Chinese Academy of SciencesBeijing100190China School of Remote Sensing and Geomatics Engineering Nanjing University of Information Science and TechnologyNanjing210044China College of Geological Engineering and Geomatics Chang’an UniversityXi’an710054China University of Electronic Science and Technology of China Chengdu611731China

The Global Navigation Satellite Systems(GNSS),including the US’s GPS,China’s BDS,the European Union’s Galileo,and Russia’s GLONASS,offer real-time,all-weather,any-time,anywhere and high precision observations by transmitting L band signals continuously,which have been widely used for positioning,navigation and timing.With the development of GNSS technology,it has been found that GNSS-reflected signals can be used to detect Earth’s surface characteristics together with other signals of opportunity.In this paper,the current status and latest advances are presented on Global Navigation Satellite System-Reflectometry(GNSS-R)in theory,methods,techniques and observations.New developments and progresses in GNSS-R instruments,theoretical modeling,and signal processing,ground and space-/air-borne experiments,parameters retrieval(e.***.wind speed,sea surface height,soil moisture,ice thickness),sea surface altimetry and applications in the atmosphere,oceans,land,vegetation,and cryosphere are given and reviewed in details.Meanwhile,the challenges in the GNSS-R development of each field are also given.Finally,the future applications and prospects of GNSS-R are discussed,including multi-GNSS reflectometry,new GNSS-R receivers,GNSS-R missions,and emerging applications,such as mesoscale ocean eddies,ocean phytoplankton blooms,microplastics detection,target recognition,river flow,desert studies,natural hazards and landslides monitoring.

关键词： GNSS Reflectometry Remote sensing Environmental change

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Evaluation of the chemical profile from four germplasms sources of Pruni Semen using UHPLC-LTQ-Orbitrap-MS and multivariate analyses

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Journal of Pharmaceutical Analysis 2022年第5期12卷 733-742页

作者： Zihan Zhao Yue Liu Yushi Zhang Zeyu Geng Rina Su Lipeng Zhou Chao Han Zhanjun Wang shuangcheng ma Weidong Li School of Chinese materia Medica Beijing University of Chinese MedicineBeijing102488China Institute of Forestry and Grassland Ecology Ningxia Academy of Agricultural and Forestry SciencesYinchuan750002China Institute for Control of Chinese Traditional Medicine and Ethnic Medicine National Institutes for Food and Drug ControlBeijing100050China

Pruni Semen,the seed of several unique Prunus plants,is a traditional purgative herbal material.To determine the authentic sources of Pruni Semen,46 samples from four species were collected and analyzed.Ten compounds including multiflorin A(Mul A),a notable purative compound,were isolated and identified by chemical separation and nuclear magnetic resonance spectroscopy.Seventy-six communal components were identified by ultra-high performance liquid chromatography with linear ion trap-quadrupole Orbitrap mass spectrometry,and acetyl flavonoid glycosides were recognized as characteristic constituents.The flavonoids were distributed in the seed coat and cyanogenic glycosides in the kernel.Based on this,methods for identifying Pruni Semen from different sources were established using chemical fingerprinting,quantitative analysis of the eight principal compounds,hierarchical cluster analysis,principal component analysis,and orthogonal partial least squares discriminant analysis.The results showed that the samples were divided into two categories:one is the small seeds from Prunus humilis(Ph)and Prunus japonica(Pj),and the other is the big seeds from Prunus pedunculata(Pp)and Prunus triloba(Pt).The average content of Mul A was 3.02,6.93,0.40,and 0.29 mg/g,while the average content of amygdalin was 18.5,17.7,31.5,and 30.9 mg/g in Ph,Pj,Pp,and Pt,respectively.All the above information suggests that small seeds might be superior sources of Pruni Semen.This is the first comprehensive report on the identification of chemical components in Pruni Semen from different species.

关键词： Pruni semen Acetyl flavonoids Amygdalin Multivariate analyses Authentic sources

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A holistic strategy for quality and safety control of traditional Chinese medicines by the “iVarious” standard system

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Journal of Pharmaceutical Analysis 2017年第5期7卷 271-279页

作者： Anzhen Chen Lei Sun Hang Yuan Aiying Wu Jingguang Lu shuangcheng ma Qingdao Institute for Food and Drug Control National Institutes for Food and Drug Control Xinjiang Institute for Food and Drug Control

An effective quality control system is the key to ensuring the quality, safety and efficacy of traditional Chinese medicines(TCMs). However, the current quality standard research lacks top-level design and systematic design,mostly based on specific technologies or evaluation methods. To resolve the challenges and questions of quality control of TCMs, a brand-new quality standard system, named "iVarious", was proposed. The system comprises eight elements in a modular format. Meaning of every element was specifically illustrated via corresponding research instances. Furthermore, frankincense study was taken as an example for demonstrating standards and research process, based on the "i Various" system. This system highlighted a holistic strategy for effectiveness,security, integrity and systematization of quality and safety control standards of TCMs. The establishment of"i Various" integrates multi-disciplinary technologies and progressive methods, basis elements and key points of standard construction. The system provides a novel idea and technological demonstration for regulation establishment of TCMs quality standards.

关键词： iVarious Quality standard system Traditional Chinese medicines (TCMs) Quality and safety control Holistic strategy

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Is it really the ＂dark side＂ of herbal medicine？

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Science China(Life Sciences) 2018年第9期61卷 1118-1119页

作者： Yang Luan shuangcheng ma Yimin mao Hongqiao International Institute of Medicine Shanghai Tong Ren Hospital and Faculty of Public Health Shanghai Jiao Tong University School of MedicineShanghai 200025 China National Institutes for Food and Drug Control Beijing 100050 China Division of Gastroenterology and Hepatology Key Laboratory of Gastroenterology and Hepatology Ministry of Health Renji HospitalShanghai Jiao Tong University School of MedicineShanghai 200127 China

Aristolochic acids (AAs), previously rarely mentioned compounds, have been brought into the public eye several times since the beginning of this century; and currently, they have drawn even greater attention due to their association with liver cancer, as was recently reported by Ng et al.(Ng et al., 2017). The authors sequenced the whole exomes of 98patients with hepatocellular carcinomas (HCC) from Taiwan and reported that 78%of the cases carried the AAs mutational signature, A:T>T:A transversion. Further examination of publicly available data from Chinese patients with

关键词：草药混合物癌症眼睛定序

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超高效液相-质谱法测定滴通鼻炎水中马兜铃酸Ⅰ、Ⅱ的含量

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中国药物警戒 2024年第4期21卷 386-390,396页

作者：郭日新肖萌刘静戴忠马双成中国食品药品检定研究院中药民族药检定所北京100050

目的测定滴通鼻炎水中马兜铃酸Ⅰ、马兜铃酸Ⅱ的含量,为其质量控制提供借鉴。方法采用超高效液相-质谱法(UPLC-MS/MS)同时测定滴通鼻炎水中马兜铃酸Ⅰ、马兜铃酸Ⅱ的含量。色谱柱采用Waters-ACQUI UPLC HSS T3 C_(18)(2.1 mm×100 mm,1.... 详细信息

目的测定滴通鼻炎水中马兜铃酸Ⅰ、马兜铃酸Ⅱ的含量,为其质量控制提供借鉴。方法采用超高效液相-质谱法(UPLC-MS/MS)同时测定滴通鼻炎水中马兜铃酸Ⅰ、马兜铃酸Ⅱ的含量。色谱柱采用Waters-ACQUI UPLC HSS T3 C_(18)(2.1 mm×100 mm,1.8μm),采用乙腈为流动相A,0.1%甲酸含1 mmol·L^(-1)乙酸铵溶液为流动相B,梯度洗脱,电喷雾离子源(ESI),正离子多反应监测模式,以标准曲线法计算含量。结果17批滴通鼻炎水中有9批样品未检出马兜铃酸Ⅰ(检出限0.13 pg),8批样品中检出马兜铃酸Ⅰ,含量在0.41~3.60 ng·mL^(-1)。所有样品中均未检出马兜铃酸Ⅱ(检出限0.41 pg)。结论建立了UPLC-MS/MS同时测定滴通鼻炎水中马兜铃酸Ⅰ、马兜铃酸Ⅱ含量的方法,该方法专属性强、灵敏度高、重复性好,可为滴通鼻炎水中马兜铃酸Ⅰ、马兜铃酸Ⅱ的质量控制提供参考。

关键词：滴通鼻炎水马兜铃酸Ⅰ 马兜铃酸Ⅱ 超高效液相-质谱法含量测定

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星载GNSS-R反演土壤湿度研究进展与思考

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武汉大学学报（信息科学版） 2024年第1期49卷 15-26页

作者：张双成郭沁雨马中民刘奇胡胜伟周昕赵贺斌长安大学地质工程与测绘学院陕西西安710054 西部矿产资源与地质工程教育部重点实验室陕西西安710054 地理信息工程国家重点实验室陕西西安710054

土壤湿度作为陆地水循环中最为活跃的部分,在陆面水分的形成、转化和消耗过程中扮演重要角色,同时它也是影响水文过程、植被状态及气候条件的关键参数。传统的直接测量法耗时耗力,无法实现大范围监测需求,而光学遥感法易受云层、植被遮... 详细信息

土壤湿度作为陆地水循环中最为活跃的部分,在陆面水分的形成、转化和消耗过程中扮演重要角色,同时它也是影响水文过程、植被状态及气候条件的关键参数。传统的直接测量法耗时耗力,无法实现大范围监测需求,而光学遥感法易受云层、植被遮蔽,微波遥感法则无法兼顾时空分辨率。首先,介绍利用全球导航卫星系统反射测量(global navigation satellite system-reflectometry,GNSS-R)技术反演土壤湿度的基本理论,并分析反演过程中出现的误差源以及现有的误差校正方法及其局限性。然后,从3个方面分析了近年来星载GNSS-R反演土壤湿度的研究进展:(1)星载GNSS-R星座发展现状;(2)星载GNSS-R反演土壤湿度的算法综述,包括经验模型、半经验模型和机器学习方法;(3)融合星载GNSS-R与其他数据反演土壤湿度的进展及方向。最后,讨论了目前利用星载GNSS-R技术反演土壤湿度所面临的技术难点,并对未来研究重点进行展望。

关键词：星载GNSS-R 土壤湿度机器学习旋风全球导航卫星系统

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抑制混排提升高镍三元材料电化学性能

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北京化工大学学报（自然科学版） 2024年第2期51卷 66-74页

作者：张绘敏赵扬黄双成河南应用技术职业学院机电工程学院郑州450042 河南应用技术职业学院化学工程学院郑州450042

层状高镍三元材料LiNi_(0.85)Co_(0.11)Mn_(0.04)O_(2)(NCM85)是一种很有前途的高性能锂离子电池正极材料。然而,正极材料在高温煅烧过程中极有可能出现Li损失和阳离子混排现象,导致正极颗粒表面形成无序的岩盐相。提出了一种用H3PO4处... 详细信息

层状高镍三元材料LiNi_(0.85)Co_(0.11)Mn_(0.04)O_(2)(NCM85)是一种很有前途的高性能锂离子电池正极材料。然而,正极材料在高温煅烧过程中极有可能出现Li损失和阳离子混排现象,导致正极颗粒表面形成无序的岩盐相。提出了一种用H3PO4处理制备等离子体修饰NCM85的方法,在NCM85表面原位形成一层均匀的Li_(3)PO_(4)包覆层,有效地提高了NCM85的电化学性能,稳定了正极-电解质界面。与本体NCM85电极相比,适量Li_(3)PO_(4)包覆层修饰的正极(Li_(3)PO_(4)@NCM85)展现出优异的电化学性能,在2.75~4.3 V电压范围内0.5 C(200 ma/g)倍率循环200圈后,放电比容量为169.2(ma·h/g),容量保持率高达84%,Li_(3)PO_(4)包覆层生成的过程中消耗了材料表面的残锂,减少了Li^(+)/Ni^(2+)的混排,增强了结构的稳定性。此外,Li_(3)PO_(4)包覆层可以提高离子电导率,加快Li+扩散速率,抑制相变、阳离子混合和体积收缩。研究了LPO表面修饰对材料界面机制的影响,对下一代高能锂离子电池正极材料的开发具有一定借鉴意义。

关键词：高镍三元材料 Li^(+)/Ni^(2+)混排 Li_(3)PO_(4)包覆层离子电导率

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