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Salinispora Arenicola CNP193新颖NRPS基因簇在Streptomycetes Coelicolor M512中的异源表达

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科学技术与工程 2017年第2期17卷 161-164页

作者：刘姝房耀维王淑军焦豫良胡晟源潘建梅江苏省海洋资源开发研究院连云港222000 淮海工学院海洋生命与水产学院连云港222005

异源表达海洋专性放线菌salinispora arenicola CNP193新颖NRPS基因簇。PCR扩增基因簇两端各约1 000 bp的片段,融合PCR将两片段融合,同质粒pC AP01双酶切后连接,构建基因簇捕获载体pC AP01-preP KS/NRPS,通过TAR(transformation-associated recombination)克隆技术构建基因簇捕获质粒pC AP01-PKS/NRPS,运用三亲接合转移法将pC AP01-PKS/NRPS转化streptomycetes coelicolor M512。HPLC检测对照菌株和重组菌发酵液乙酸乙酯提取物初步表明基因簇有表达产物产生。为新颖salinispora arenicola CNP193新颖NRPS基因簇的鉴定奠定基础。

关键词：海洋专性放线菌基因簇异源表达

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维特根斯坦之梯:“可说”与“不可说”之论

齐齐哈尔大学学报（哲学社会科学版） 2019年第2期 30-32页

作者：方淑瑶赵妙法安徽大学哲学系安徽合肥230039

"可说"与"不可说"是维特根斯坦《逻辑哲学论》中的两个重要概念。"可说"是指可以说清楚的、有意义的命题,主要是自然科学命题。"不可说"主要包括"伦理"、"美学"、"形而上学"等不表达关于世界的事实的东西。维特根斯坦认为,凡是不可说的... 详细信息

"可说"与"不可说"是维特根斯坦《逻辑哲学论》中的两个重要概念。"可说"是指可以说清楚的、有意义的命题,主要是自然科学命题。"不可说"主要包括"伦理"、"美学"、"形而上学"等不表达关于世界的事实的东西。维特根斯坦认为,凡是不可说的东西必须保持静默。维特根斯坦关于不可说东西的言说具有类似于梯子的作用,是在更高层次上进行的。人们借助于梯子便可以清楚地看见究竟什么是可说的,什么是不可说的。维特根斯坦认为,只有进入这种境界的人,才能真正领悟"不可说"的真谛——即抛开这架梯子。本文主要探讨维特根斯坦的"可说"与"不可说"思想的内涵,并且对它们的界限进行了划分,最终达到一种超越的境界。

关键词：《逻辑哲学论》可说不可说梯子

Mediating role of health literacy in the relationship between social isolation and psychological distress among pre-frail older adults

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World Journal of Psychiatry 2025年第1期15卷 137-144页

作者： Juan fang Li-Hui Li Ming-Qian He Yue Ji Dong-Yan Lu Li-Bing Zhang Jin-Lan yao School of Medicine Huzhou UniversityHuzhou 313000Zhejiang ProvinceChina

BACKGROUND Frailty has become a significant public health *** recent increase in the number of frail older adults has led to increased attention being paid to psycho-logical care services in *** social isolation of pre-frail older adults can impact their psychological *** To explore the mediating effect of health literacy between social isolation and psychological distress among communitydwelling older adults with *** This descriptive cross-sectional study was conducted with 254 pre-frail older individuals aged 60 years and *** isolation,health literacy,and psycholo-gical distress were comprehensively measured using the Lubben Social Network Scale-6,12-item Short-Form Health Literacy Questionnaire,and the Kessler Psy-chological Distress *** were evaluated using the SPSS 27.0 package program and the PROCESS macro *** statistical analyses,correlation analyses,and bootstrap mediation tests were used to assess associations between the *** The results showed that social isolation had an effect on health literacy among pre-frail older adults(β=0.240,P<0.001),social isolation impact on psycho-logical distress pre-frail older adults(β=-0.415,P<0.001);health literacy was identified effect on psychological distress among pre-frail older persons(β=-0.307,P<0.001).Health literacy partially mediated the relationship between social isolation and psychological distress among community-dwelling older adults with pre-frailty,with a mediation effect of-0.074,accounting for 17.83%of the total effect.

关键词： Frail Ageing Psychological distress Health literacy Social isolation

A cellulose-based lithium-ion battery separator with regulated ionic transport and high thermal stability for extreme environments

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Nano Research 2025年第1期18卷 315-322页

作者： Kun-Peng Yang Song Xie Zi-Meng Han Hao-Cheng Liu Chong-Han Yin Wen-Bin Sun Zhang-Chi Ling Huai-Bin Yang De-Han Li Qing-fang Guan shu-Hong Yu Department of Chemistry New Cornerstone Science LaboratoryInstitute of Biomimetic Materials&ChemistryAnhui Engineering Laboratory of Biomimetic MaterialsDivision of Nanomaterials&ChemistryHefei National Research Center for Physical Sciences at the MicroscaleUniversity of Science and Technology of ChinaHefei 230026China Institute of Innovative Materials Department of Materials Science and EngineeringDepartment of ChemistrySouthern University of Science and TechnologyShenzhen 518055China

Separators play a critical role in lithium-ion ***,the restrictions of thermal stability and inferior electrical performance in commercial polyolefin separators significantly limit their applications under harsh ***,we report a cellulose-assisted self-assembly strategy to construct a cellulose-based separator massively and *** an ultrahigh ionic conductivity in electrolytes of 3.7 mS·cm^(-1) and the ability to regulate ion transport,the obtained separator is a promising alternative for high-performance lithium-ion *** addition,integrated with high thermal stability,the cellulose-based separator endows batteries with high safety at high temperatures,greatly expanding the application scenarios of energy storage devices in extreme environments.

关键词： lithium-ion batteries bio-inspired materials battery separators cellulose nanofiber ultrahigh ionic conductivity high safety

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干细胞的趋电性迁移及机制

中国组织工程研究 2025年第23期29卷 4984-4992页

作者：韩芳舒晴贾绍辉田峻武汉体育学院运动医学院湖北省武汉市430079 武汉大学中南医院骨科康复科湖北省武汉市430071

背景:随着对干细胞技术研究的深入,如何使其准确归巢成为临床应用中的一大难题。除药物和趋化因子等信号的诱导外,电场也被广泛应用于指导干细胞的定向迁移,并可增强其迁移速度和定向性。目的:旨在分析总结电场对干细胞迁移特性的影响,... 详细信息

背景:随着对干细胞技术研究的深入,如何使其准确归巢成为临床应用中的一大难题。除药物和趋化因子等信号的诱导外,电场也被广泛应用于指导干细胞的定向迁移,并可增强其迁移速度和定向性。目的:旨在分析总结电场对干细胞迁移特性的影响,同时综述可能的作用机制。方法:通过检索Pub Med和中国知网数据库,收集截至2024年3月的相关文献,英文检索词:“stem cells,direct current electric field,pulsed electric field,migration,electric field device,mechanism”;中文检索词:“干细胞,直流电场,脉冲电场,迁移,电场装置,机制”。排除不能获取全文和与主题无关的文献。结果与结论:根据筛选要求共纳入58篇文献,包括中文文献15篇及英文文献43篇。文献以脂肪间充质干细胞、骨髓间充质干细胞、神经干细胞、表皮干细胞、人胚胎干细胞和人诱导性多功能干细胞为研究对象,在迁移装置中研究电场的不同参数对上述干细胞迁移的影响及其机制。(1)电场作为一种简单、无创且稳定的干预方式在指导干细胞定向迁移的过程中起着积极作用;(2)不同类型的干细胞发生趋电性迁移的方向不同,同时大部分干细胞的迁移速度和定向性是随着电场强度增加而增加的;(3)不同的电场装置在观测干细胞迁移时的侧重点不同,可根据实验目的而择优选择相关装置;(4)不同干细胞趋电性迁移的机制不完全相同,多数干细胞迁移过程中有MAPK通路、ROCK活化以及PI3K功能的参与,同时还有其他蛋白质复合物与信号通路参与调控该过程;(5)除电场参数不同之外,细胞本身的衰老情况和培养环境也会对趋电性迁移的结果产生影响。总而言之,电场作为一种影响干细胞迁移特性的重要信号,与其他新兴材料结合在组织工程应用中展现出了一定的潜力,有望在指导干细胞归巢方面发挥更加重要的作用,促进骨组织再生和修复以及神经系统、自身免疫系统以及肿瘤等疾病的研究取得更大的突破。

关键词：干细胞电场趋电性迁移机制归巢效应物理因子电场装置细胞衰老培养环境

不同热处理蛋黄脂质对小鼠学习认知能力下降的缓解能力

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食品工业科技 2025年第1期46卷 367-376页

作者：吴瑶刘铭坤方冰张明合肥工业大学食品与生物工程学院安徽合肥230061 中国农业大学营养与健康系北京100083 北京工商大学食品与健康学院北京100048

为探讨不同温度处理下蛋黄脂质对小鼠学习认知能力下降的缓解能力的差异。本研究利用小鼠认知障碍模型,比较95℃(水浴30 min)和121℃(灭菌锅加热30 min)热处理蛋黄脂质组分改善小鼠学习认知能力的差异。研究结果表明,相较于121℃热处理... 详细信息

为探讨不同温度处理下蛋黄脂质对小鼠学习认知能力下降的缓解能力的差异。本研究利用小鼠认知障碍模型,比较95℃(水浴30 min)和121℃(灭菌锅加热30 min)热处理蛋黄脂质组分改善小鼠学习认知能力的差异。研究结果表明,相较于121℃热处理的蛋黄脂质,95℃热处理的蛋黄脂质更有效地降低了Tau蛋白的磷酸化和Aβ1-40、GSK-3β表达,下调了促炎因子的表达,其提升小鼠的学习记忆能力的效果更好。95℃热处理蛋黄脂质中的多不饱和脂肪酸以及DHA较121℃热处理的蛋黄脂质显著增加(P<0.05)。因此,过度的加热条件,会破坏蛋品中DHA等多不饱和脂肪酸,削弱蛋黄脂质的改善认知的生物活性。本研究为蛋制品的热加工工艺的优化提供参考依据。

关键词：蛋黄脂质热处理学习和记忆

Increased international normalized ratio level in hepatocellular carcinoma patients with diabetes mellitus

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World Journal of Gastroenterology 2013年第15期19卷 2395-2403页

作者： Hui Zhang Chun Gao Long fang shu-Kun yao Department of Gastroenterology China-Japan Friendship HospitalMinistry of Health

AIM: To determine the association of diabetes mellitus (DM) and international normalized ratio (INR) level in hepatocellular carcinoma (HCC) patients. METHODS: Our present study included 375 HCC patients who were treated at the China-Japan Friendship Hospital, Ministry of Health (Beijing, China), in the period from January 2003 to April 2012, and with a hospital discharge diagnosis of HCC. The demographic, clinical, laboratory, metabolic and instrumental features were analyzed. χ2 test, Student's t test and Mann-Whitney U test were used to compare the differences between HCC patients with and without DM. Unconditional multivariable logistic regression analysis was used to determine the association of DM and INR level in HCC patients. A sub-group analysis was performed to assess the effect of liver cirrhosis or hepatitis B virus (HBV) infection on the results. The Pearson correlation test was used to determine the relationship between INR level and fasting glucose. In addition, association between diabetes duration, and diabetes treatment and INR level was determined considering the potentially different effects. RESULTS: Of the total, 63 (16.8%) patients were diabetic (diabetic group) and 312 (83.2%) patients were diagnosed without diabetes (non-diabetic group). Their mean age was 56.4 ± 11.0 years and 312 (83.2%) patients were male. Compared with patients without DM, the HCC patients with diabetes were older (59.5 ± 10.3 vs 55.8 ± 11.1, P=0.015), had a lower incidence of HBV infection (79.4% vs 89.1%, P=0.033), had increased levels of systolic blood pressure (SBP) (133 ± 17 vs 129 ± 16 mmHg, P=0.048) and INR (1.31 ± 0.44 vs 1.18 ± 0.21, P=0.001), had lower values of hemoglobin (124.4 ± 23.9 vs 134.2 ± 23.4, P=0.003) and had a platelet count (median/interquartile-range: 113/64-157 vs 139/89-192, P=0.020). There was no statistically significant difference in the percentages of males, overweight or obesity, drinking, smoking, cirrhosis and Child classification. After

关键词： International normalized ratio Coagulation function Diabetes mellitus Hepatocellular carcinoma Chinese patients

Degradation of atrazine and changes in soil biological indices throughout dendroremediation using poplars

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Journal of Forestry Research 2019年第6期30卷 2331-2339页

作者： Bin yao Xuejing Gu Ying-ge shu fang Bao Yonghua Li Institute of Desertification Studies Chinese Academy of ForestryBeijing 100091People’s Republic of China Beijing Municipal Research Institute of Environmental Protection Beijing 100037People’s Republic of China Ecology and Environment Department of Guizhou University Guiyang 550025People’s Republic of China

Dendroremediation,a novel strategy for cleaning up contaminants from the environment by using special woody plant species and microbes,is a popular phytoremediation *** is a cost-effective and environmentally friendly method that is being increasingly *** species(Populus L.)have been suggested for use in remediation because of their characteristics of high biomass production,fast growth,and potential for removing *** environment is often contaminated by toxic substances produced by human activities,and remediation of contamination is therefore a global *** is one of the most widely used herbicides in *** the present study,‘I-69/55’poplar(*** cv.‘I-69/55’)and hybrid poplar(Populus deltoides×nigra,DN34)grown in pots were assayed for their ability to remediate atrazine-contaminated *** degradation dynamics of atrazine were assessed with a high-performance liquid chromatography system using materials from a greenhouse with different rhizosphere *** studied the dynamic variation in microbes and microbial biomass carbon to elucidate the rhizosphere effects and mechanism of remediation of atrazine-contaminated soil by *** four treatments included a control with uncontaminated soil without a tree,atrazine pollution without a tree,atrazine pollution with‘I-69/55’poplar,and atrazine pollution with hybrid *** poplar showed potential for remediation of atrazine-contaminated soil,and the degradation of atrazine in the rhizosphere was faster than that in non-rhizosphere *** significantly inhibited bacteria growth in nonrhizosphere *** high concentration of bacteria in the hybrid poplar rhizosphere might be key to atrazine *** of change among fungi and actinomyces did not correspond to trends in atrazine degradation throughout the trial *** research is needed to predict the effects of atrazine on cultivable microorganisms in various *** had s

关键词： Atrazine Dendroremediation Poplar Biodegradation Microbes

A learning theory of meta learning

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National Science Review 2024年第8期11卷 9-10页

作者： fang yao School of Mathematical Sciences Peking University

A key feature of human intelligence is the ability to transfer knowledge learned from multiple learning tasks to other similar but different ones. Namely, humans attempt to learn how to generalize, and thus they can tackle a continuous stream of learning tasks. Correspondingly, in real-world applications, it is always crucial to improve learning in a new task by leveraging knowledge transferred from related tasks that have already been learned.

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