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Simulation and fabrication of in vitro microfluidic microelectrode array chip for patterned culture and electrophysiological detection of neurons

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Nanotechnology and Precision Engineering 2024年第2期7卷 1-10页

作者： Yan Yang Shihong Xu Yu Deng Yaoyao Liu Kui Zhang shiya lv Longze Sha Qi Xu Xinxia Cai Jinping Luo State Key Laboratory of Transducer Technology Aerospace Information Research InstituteChinese Academy of SciencesBeijing 100190China School of Electronic Electrical and Communication EngineeringUniversity of Chinese Academy of SciencesBeijing 100049China State Key Laboratory of Medical Molecular Biology Institute of Basic Medical SciencesChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100005China

To enable the detection and modulation of modularized neural networks in vitro,this study proposes a microfluidic microelectrode array chip for the cultivation,compartmentalization,and control of neural *** chip was designed based on the specific structure of neurons and the requirements for detection and ***-element analysis of the chip’s flow field was conducted using the COMSOL Multiphysics software,and the simulation results show that the liquid within the chip can flow smoothly,ensuring stable flow fields that facilitate the uniform growth of neurons within the microfluidic *** employing MEMS technology in combination with nanomaterial modification techniques,the microfluidic microelectrode array chip was fabricated *** hippocampal neurons were cultured on the chip,forming a well-defined neural *** electrical activity of the detected neurons was recorded,exhibiting a 23.7%increase in amplitude compared to neuronal discharges detected on an open-field microelectrode *** study provides a platform for the precise detection and modulation of patterned neuronal growth in vitro,potentially serving as a novel tool in neuroscience research.

关键词： Microfluidic Microelectrode array In vitro Neural chip

Effect of Shengmai Yin on the DNA methylation status of nasopharyngeal carcinoma cell and its radioresistant strains

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Journal of Pharmaceutical Analysis 2021年第6期11卷 783-790页

作者： shiya Liu Zhiyuan Wang Daoqi Zhu Jiabin Yang Dandan Lou Ruijiao Gao Zetai Wang Aiwu Li Ying lv Qin Fan School of Traditional Chinese Medicine Southern Medical UniversityGuangzhou510515China Nan Fang Hospital Guangzhou510515China

Shengmai Yin(SMY)is a Chinese herbal decoction that effectively alleviates the side effects of radiotherapy in various cancers and helps achieve radiotherapy’s clinical *** this study,we explored the interaction mechanism among SMY,DNA methylation,and nasopharyngeal carcinoma(NPC).We identified differences in DNA methylation levels in NPC CNE-2 cells and its radioresistant cells(CNE-2R)using the methylated DNA immunoprecipitation array and found that CNE-2R cells showed genomewide changes in methylation status towards a state of *** may restore its original DNA methylation status,and thus,enhance ***,we confirmed that the differential gene Tenascin-C(TNC)was overexpressed in CNE-2R cells and that SMY downregulated TNC *** downregulation of TNC inhibited NPC cell radiation resistance,migration,and ***,we found that TNC was hypomethylated in CNE-2R cells and partially restored to a hypermethylated state after SMY *** methyltransferases 3 a may be the key protein in DNA methylation of TNC.

关键词： Nasopharyngeal carcinoma DNA methylation Radiosensitization Shengmai Yin

生脉注射液对人鼻咽癌CNE-2细胞抗拒株的放射增敏作用

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世界科学技术-中医药现代化 2020年第3期22卷 804-809页

作者：刘诗雅朱道琦杨家彬娄丹丹高瑞娇吕英范钦南方医科大学中医药学院分子生物实验室广州510515 南方医科大学南方医院古中医科广州510515

目的探讨生脉注射液对人鼻咽癌CNE-2细胞抗拒株及其裸鼠移植瘤的放射增敏作用以及对STAT3、p-STAT3蛋白表达的影响。方法通过细胞毒性实验筛选生脉注射液的最佳浓度,克隆形成实验检测用药前后放射敏感性;建立人鼻咽癌裸鼠移植瘤模型,荷... 详细信息

目的探讨生脉注射液对人鼻咽癌CNE-2细胞抗拒株及其裸鼠移植瘤的放射增敏作用以及对STAT3、p-STAT3蛋白表达的影响。方法通过细胞毒性实验筛选生脉注射液的最佳浓度,克隆形成实验检测用药前后放射敏感性;建立人鼻咽癌裸鼠移植瘤模型,荷瘤鼠随机分为空白组,生脉注射液低、高剂量组,生脉注射液低、高剂量联合照射组,照射组和姜黄素联合照射对照组。计算肿瘤体积抑制率和抑瘤率。蛋白免疫印迹法检测基因表达。结果生脉注射液给药后,CNE-2R细胞放射敏感性增加;与单纯照射组比较,高剂量联合照射组肿瘤体积抑制率及抑瘤率明显升高,其STAT3、p-STAT3表达亦显著下调。结论生脉注射液对人鼻咽癌CNE-2细胞抗拒株CNE-2R及其裸鼠移植瘤具有放射增敏作用,其增敏作用可能与抑制STAT3表达与活化相关。

关键词：鼻咽癌裸鼠移植瘤生脉注射液放射增敏

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光照强度对狐臭柴叶片形态与结构的影响

贵州农业科学 2015年第6期43卷 175-178页

作者：刘诗雅张明生高晓峰刘贵贤韦红边吕享贵州大学生命科学学院贵州贵阳550025

为狐臭柴人工种植技术的构建与开发利用提供依据,采用人工设施栽培方法,研究不同光照条件下狐臭柴叶片的形态和解剖结构。结果表明:在一定光照强度范围内,随着光照强度的增加,叶面积逐渐减小,叶片颜色变浅,叶片上表皮细胞密度和叶脉直... 详细信息

为狐臭柴人工种植技术的构建与开发利用提供依据,采用人工设施栽培方法,研究不同光照条件下狐臭柴叶片的形态和解剖结构。结果表明:在一定光照强度范围内,随着光照强度的增加,叶面积逐渐减小,叶片颜色变浅,叶片上表皮细胞密度和叶脉直径均显著增大;遮光处理下叶片的叶形、叶缘、叶尖、叶质、上表皮细胞形态等指标变化与对照差异明显;遮光处理下叶片总厚度、上表皮厚度、栅栏组织厚度和海绵组织厚度较对照存在极显著差异。叶片下表皮厚度、下表皮细胞形态和下表皮细胞密度受光照强度的影响不大。

关键词：狐臭柴光照强度叶片形态叶片结构

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新型即食肉糜菲律宾蛤仔的研制

农产品加工（下） 2018年第3期 5-9页

作者：吕学姣李诗雅韩青祁艳霞吴艳秋李智博大连海洋大学食品科学与工程学院辽宁省水产品加工及综合利用重点实验室辽宁大连116023 辽宁职业学院辽宁铁岭112001

以菲律宾蛤仔为原料,研制新型肉糜即食产品。采用木瓜蛋白酶嫩化蛤肉,酶解温度50℃,酶解时间60 min,加酶量0.10%,p H值7.5。经过嫩化后的蛤肉与其他辅料进行复配,最后确定的产品生产工艺为蛤肉∶鱼肉∶猪肉质量比为8∶8∶4(以100 g混合... 详细信息

以菲律宾蛤仔为原料,研制新型肉糜即食产品。采用木瓜蛋白酶嫩化蛤肉,酶解温度50℃,酶解时间60 min,加酶量0.10%,p H值7.5。经过嫩化后的蛤肉与其他辅料进行复配,最后确定的产品生产工艺为蛤肉∶鱼肉∶猪肉质量比为8∶8∶4(以100 g混合肉糜为单位),加入蛋清15 g,大豆分离蛋白5 g,小麦面粉3 g,马铃薯淀粉3 g,香菇碎丁3 g,食盐1 g,将混合肉糜灌入清洗过后大小适宜且均一的菲律宾蛤仔外壳中,蒸制30 min后进行真空包装,最后在115℃条件下进行高温蒸汽灭菌20 min。该菲律宾蛤仔肉糜即食产品的形式新颖、营养丰富、组织结构、口感俱佳,适合进一步推广应用。

关键词：菲律宾蛤仔嫩化工艺即食产品