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two-dimensional gel electrophoresis in bacterial proteomics

Protein & Cell 2012年第5期3卷 346-363页

作者： shirly o.t.curreem.rory m.watt Susanna K.P.Lau Patrick C.Y.Woo Department of microbiology The University of Hong KongQueen Mary HospitalHong KongChina oral Biosciences Faculty of DentistryThe University of Hong KongQueen Mary HospitalHong KongChina State Key Laboratory of Emerging Infectious Diseases Department of MicrobiologyThe University of Hong KongQueen Mary HospitalHong KongChina Research Centre of Infection and Immunology The University of Hong KongQueen Mary HospitalHong KongChina Carol Yu Centre of Infection The University of Hong KongHong KongChina

two-dimensional gel electrophoresis(2-DE)is a gel-based technique widely used for analyzing the pro-tein composition of biological *** is capable of resolving complex mixtures containing more than a thousand protein components into individual protein spots through the coupling of two orthogonal bio-physical separation techniques:isoelectric focusing(first dimension)and polyacrylamide gel electrophore-sis(second dimension).2-DE is ideally suited for ana-lyzing the entire expressed protein complement of a bacterial cell:its *** relative simplicity and good reproducibility have led to 2-DE being widely used for exploring proteomics within a wide range of envi-ronmental and medically-relevant *** we give a broad overview of the basic principles and his-torical development of gel-based proteomics,and how this powerful approach can be applied for studying bacterial biology and *** highlight specific 2-DE applications that can be used to analyze when,where and how much proteins are *** links between proteomics,genomics and mass spectrometry are *** explore how proteomics involving tandem mass spectrometry can be used to analyze(post-translational)protein modifications or to identify proteins of unknown origin by de novo peptide *** use of proteome fractionation tech-niques and non-gel-based proteomic approaches are also *** highlight how the analysis of pro-teins secreted by bacterial cells(secretomes or exo-proteomes)can be used to study infection processes or the immune *** review is aimed at non-specialists who wish to gain a concise,compre-hensive and contemporary overview of the nature and applications of bacterial proteomics.

关键词： two-dimensional gel electrophoresis bacte-ria proteomics

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A history of high-power laser research and development in the United Kingdom

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High Power Laser Science and Engineering 2021年第2期9卷 41-126页

作者： Colin N.Danson malcolm White John R.m.Barr thomas Bett Peter Blyth David Bowley Ceri Brenner Robert J.Collins Neal Croxford A.E.Bucker Dangor Laurence Devereux Peter E.Dyer Anthony Dymoke-Bradshaw Christopher B.Edwards Paul Ewart Allister I.Ferguson John m.Girkin Denis R.Hall David C.Hanna Wayne Harris David I.Hillier Christopher J.Hooker Simon m.Hooker Nicholas Hopps Janet Hull David Hunt Dino A.Jaroszynski mark Kempenaars Helmut Kessler Sir Peter L.Knight Steve Knight Adrian Knowles Ciaran L.S.Lewis Ken S.Lipton Abby Littlechild John Littlechild Peter maggs Graeme P.A.malcolm oBE Stuart P.D.mangles William martin Paul mcKenna Richard o.moore Clive morrison Zulfikar Najmudin David Neely Geoff H.C.New michael J.Norman ted Paine Anthony W.Parker rory R.Penman Geoff J.Pert Chris Pietraszewski Andrew Randewich Nadeem H.Rizvi Nigel Seddon mBE Zheng-ming Sheng David Slater Roland A.Smith Christopher Spindloe Roy taylor Gary thomas John W.G.tisch Justin S.Wark Colin Webb S.mark Wiggins Dave Willford trevor Winstone AWE Aldermaston ReadingUK oxford Centre for High Energy Density Science Department of PhysicsClarendon LaboratoryUniversity of OxfordOxfordUK Centre for Intertial Fusion Studies Blackett LaboratoryImperial College LondonLondonUK Formerly of Electrox LetchworthUK Formerly of Central Laser Facility Rutherford LaboratoryDidcotUK Formerly of Ferranti Defence Systems LincolnUK Leonardo EdinburghUK Retired AWEReadingUK Formerly of AWE ReadingUK Formerly of Laser Lines Ltd BanburyUK Formerly of optilas Ltd Milton KeynesUK Retired Pro-Lite TechnologyCranfieldUK Specialised Imaging Ltd PitstoneUK Central Laser Facility STFC Rutherford Appleton LaboratoryDidcotUK magna-Power Electronics Ltd ReadingUK DeBe Lasers Needham Laser TechnologiesWhitchurchUK Blackett Laboratory Imperial College LondonLondonUK SPIE Europe CardiffUK Professor Emeritus(Physics) University of HullKingston upon HullUK Kentech Instruments Ltd WallingfordUK Department of Physics Clarendon LaboratoryUniversity of OxfordOxfordUK Professor Emeritus Department of PhysicsUniversity of StrathclydeGlasgowUK Department of Physics Durham UniversityDurhamUK Professor Emeritus(Photonics) Heriot-Watt UniversityEdinburghUK Professor Emeritus University of SouthamptonSouthamptonUK optical Surfaces Ltd KenleyUK JEH Lasers Ltd RugbyUK Scottish Universities Physics Alliance Department of PhysicsUniversity of StrathclydeGlasgowUK ItER organization Saint-Paul-lez-DuranceFrance manx Precision optics Ltd BallasallaUK Laser Lines Ltd BanburyUK Elliot Scientific Ltd MetroTest Scientific GroupHarpendenUK Centre for Plasma Physics Queen’s University BelfastBelfastUK Retired Founderand former MDRofin-Sinar UK LtdHullUK Heraeus Noblelight Ltd CambridgeUK mirli Books ChelmsfordUK m Squared Lasers Ltd GlasgowUK University of Hertfordshire HatfieldUK E&Eo UK Ltd Barton-upon-HumberUK Department of Physics University of YorkYorkUK IC optical Systems Ltd BeckenhamUK Laser micromachining Ltd St AsaphUK mBDA UK Ltd Brist

the first demonstration of laser action in ruby was made in 1960 by *** of Hughes Research Laboratories,*** laboratories worldwide began the search for lasers using different materials,operating at different *** the UK,academia,industry and the central laboratories took up the challenge from the earliest days to develop these systems for a broad range of *** historical review looks at the contribution the UK has made to the advancement of the technology,the development of systems and components and their exploitation over the last 60 years.

关键词： high-power lasers history United Kingdom

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